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O54774 (AP3D1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-3 complex subunit delta-1
Alternative name(s):
AP-3 complex subunit delta
Adapter-related protein complex 3 subunit delta-1
Delta-adaptin
Short name=mBLVR1
Gene names
Name:Ap3d1
Synonyms:Ap3d
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1199 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes By similarity. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. Ref.8

Subunit structure

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2) By similarity. AP-3 associates with the BLOC-1 complex.

Subcellular location

Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Contains 9 HEAT repeats.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasm
Golgi apparatus
Membrane
   DomainCoiled coil
Repeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanterograde axon cargo transport

Inferred from mutant phenotype Ref.8. Source: UniProtKB

anterograde synaptic vesicle transport

Inferred from mutant phenotype Ref.8. Source: UniProtKB

antigen processing and presentation

Inferred from mutant phenotype PubMed 14557411. Source: MGI

antigen processing and presentation, exogenous lipid antigen via MHC class Ib

Inferred from mutant phenotype PubMed 14557411. Source: MGI

intracellular protein transport

Inferred from mutant phenotype PubMed 14557411. Source: MGI

positive regulation of NK T cell differentiation

Inferred from mutant phenotype PubMed 14557411. Source: MGI

protein localization to organelle

Inferred from mutant phenotype PubMed 15860731. Source: MGI

regulation of sequestering of zinc ion

Traceable author statement PubMed 17349999. Source: BHF-UCL

synaptic vesicle membrane organization

Inferred from mutant phenotype PubMed 15860731. Source: MGI

vesicle-mediated transport

Traceable author statement PubMed 9714600. Source: MGI

zinc ion transport

Traceable author statement PubMed 17349999. Source: BHF-UCL

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

axon

Inferred from direct assay PubMed 20089890. Source: MGI

endosome membrane

Inferred from direct assay PubMed 16162817. Source: UniProtKB

membrane coat

Inferred from electronic annotation. Source: InterPro

terminal bouton

Inferred from direct assay PubMed 20089890. Source: MGI

trans-Golgi network

Traceable author statement PubMed 9714600. Source: MGI

   Molecular_functionprotein transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 11991198AP-3 complex subunit delta-1
PRO_0000193767

Regions

Repeat34 – 7138HEAT 1
Repeat142 – 17938HEAT 2
Repeat180 – 21637HEAT 3
Repeat218 – 25437HEAT 4
Repeat257 – 29640HEAT 5
Repeat298 – 33639HEAT 6
Repeat337 – 37337HEAT 7
Repeat375 – 40935HEAT 8
Repeat521 – 55838HEAT 9
Coiled coil659 – 67921 Potential
Coiled coil722 – 75029 Potential
Coiled coil843 – 86321 Potential
Coiled coil911 – 93424 Potential
Compositional bias836 – 944109Lys-rich
Compositional bias956 – 9605Poly-Glu

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue6321Phosphoserine Ref.4 Ref.6
Modified residue6341Phosphoserine Ref.4 Ref.6
Modified residue6361Phosphoserine Ref.4 Ref.6
Modified residue6881Phosphoserine By similarity
Modified residue7541Phosphoserine By similarity
Modified residue7551Phosphoserine Ref.4 Ref.6
Modified residue7581Phosphothreonine Ref.4 Ref.6 Ref.7
Modified residue7601Phosphoserine Ref.4 Ref.5 Ref.6 Ref.7
Modified residue7841Phosphoserine Ref.4 Ref.6
Modified residue8251Phosphoserine Ref.3 Ref.4

Sequences

Sequence LengthMass (Da)Tools
O54774 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 29D2D036B36C3B05

FASTA1,199135,081
        10         20         30         40         50         60 
MALKMVKGSI DRMFDKNLQD LVRGIRNHKE DEAKYISQCI DEIKQELKQD NIAVKANAVC 

        70         80         90        100        110        120 
KLTYLQMLGY DISWAAFNII EVMSASKFTF KRVGYLAASQ CFHEGTDVIM LTTNQIRKDL 

       130        140        150        160        170        180 
SSPSQYDTGV ALTGLSCFVT PDLARDLAND IMTLMSHTKP YIRKKAVLIM YKVFLKYPES 

       190        200        210        220        230        240 
LRPAFPRLKE KLEDPDPGVQ SAAVNVICEL ARRNPKNYLS LAPLFFKLMT SSTNNWVLIK 

       250        260        270        280        290        300 
IIKLFGALTP LEPRLGKKLI EPLTNLIHST SAMSLLYECV NTVIAVLISL SSGMPNHSAS 

       310        320        330        340        350        360 
IQLCVQKLRI LIEDSDQNLK YLGLLAMSKI LKTHPKSVQS HKDLILQCLD DKDESIRLRA 

       370        380        390        400        410        420 
LDLLYGMVSK KNLMEIVKKL MTHVDKAEGT TYRDELLTKI IDICSQSNYQ HITNFEWYIS 

       430        440        450        460        470        480 
ILVELTRLEG TRHGHLIAAQ MLDVAIRVKA IRKFAVSQMS SLLDSAHLVA SSTQRNGICE 

       490        500        510        520        530        540 
VLYAAAWICG EFSEHLQGPQ QTLEAMLRPK VTTLPGHIQA VYVQNVVKLY ASILQQKEQA 

       550        560        570        580        590        600 
ADTEAAQEVT QLLVERLPQF VQSADLEVQE RASCILQLVK HVQKLQAKGV PVAEEVSALF 

       610        620        630        640        650        660 
AGELNPVAPK AQKKVPVPEG LDLDAWINEP PSDSESEDEK PKAIFHEEEP RHTRRRQPEE 

       670        680        690        700        710        720 
DEEELARRRE ARKQEQANNP FYIKSSPSPQ KRYQDAPGVE HIPVVQIDLS VPLKVPGMPM 

       730        740        750        760        770        780 
SDQYVKLEEQ RRHRQRLEKD KKRKKKEKGK RRHSSLPTES DEDIAPAQRV DIITEEMPEN 

       790        800        810        820        830        840 
ALPSDEDDKD PNDPYRALDI DLDKPLADSE KLPVQKHRNA EAVKSPEKEG VLGVEKKSKK 

       850        860        870        880        890        900 
PKKKEKKTKE REREKKDKKG EDLDFWLSTT PPPAAAPIPA PSTEELAAST ITSPKDECEV 

       910        920        930        940        950        960 
LKGEEEDHVD HDQERKSSRH KKKKHRKEKE KEERPRDKKK AKKKQVAPLE NGAAAEEEEE 

       970        980        990       1000       1010       1020 
PIPPMSSYCL LAESPYIKVT YDIQASLQKD SQVTVSIILE NQSSSFLKNM ELNVLDSLNT 

      1030       1040       1050       1060       1070       1080 
KMTRPEGSSV HDGVPVPFQL PPGVSNEAQF VFTIQSIVMA QKLKGTLSFI AKDDEGATHE 

      1090       1100       1110       1120       1130       1140 
KLDFRLHFSC SSYLITTPCY SDAFAKLLES GDLSMNSIKV DGISMSFQNL LAKICFYHHF 

      1150       1160       1170       1180       1190 
SVVERVDSCA SMYSRSIQGH HVCLLVKKGE SSVSVDGKCS DATLLSSLLE EMKTTLAQC 

« Hide

References

« Hide 'large scale' references
[1]"The mouse homolog of the bovine leukemia virus receptor is closely related to the delta subunit of adaptor-related protein complex AP-3, not associated with the cell surface."
Suzuki T., Ikeda H.
J. Virol. 72:593-599(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain and Eye.
[3]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632; SER-634; SER-636; SER-755; THR-758; SER-760; SER-784 AND SER-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[6]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632; SER-634; SER-636; SER-755; THR-758; SER-760 AND SER-784, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-758 AND SER-760, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[8]"The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB004305 mRNA. Translation: BAA24578.1.
BC048786 mRNA. Translation: AAH48786.1.
BC053066 mRNA. Translation: AAH53066.1.
PIRT13946.
RefSeqNP_031486.1. NM_007460.1.
UniGeneMm.209294.

3D structure databases

ProteinModelPortalO54774.
SMRO54774. Positions 11-614, 697-729.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198134. 5 interactions.
DIPDIP-32163N.
IntActO54774. 2 interactions.
MINTMINT-4104952.

PTM databases

PhosphoSiteO54774.

Proteomic databases

PaxDbO54774.
PRIDEO54774.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020420; ENSMUSP00000020420; ENSMUSG00000020198.
GeneID11776.
KEGGmmu:11776.
UCSCuc007gek.1. mouse.

Organism-specific databases

CTD8943.
MGIMGI:107734. Ap3d1.

Phylogenomic databases

eggNOGCOG5096.
GeneTreeENSGT00550000075067.
HOGENOMHOG000000981.
HOVERGENHBG050520.
InParanoidO54774.
KOK12396.
OMAKANAVCK.
OrthoDBEOG79SDWG.
PhylomeDBO54774.
TreeFamTF105666.

Gene expression databases

BgeeO54774.
GenevestigatorO54774.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
InterProIPR017105. AP3_complex_dsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR010474. BLV_receptor.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamPF01602. Adaptin_N. 1 hit.
PF06375. BLVR. 1 hit.
[Graphical view]
PIRSFPIRSF037092. AP3_complex_delta. 1 hit.
SUPFAMSSF48371. SSF48371. 1 hit.
ProtoNetSearch...

Other

ChiTaRSAP3D1. mouse.
NextBio279571.
PROO54774.
SOURCESearch...

Entry information

Entry nameAP3D1_MOUSE
AccessionPrimary (citable) accession number: O54774
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot