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Protein

AP-3 complex subunit delta-1

Gene

Ap3d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes (By similarity). In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.By similarity1 Publication

GO - Biological processi

  1. anterograde axon cargo transport Source: UniProtKB
  2. anterograde synaptic vesicle transport Source: UniProtKB
  3. antigen processing and presentation Source: MGI
  4. antigen processing and presentation, exogenous lipid antigen via MHC class Ib Source: MGI
  5. endosome to melanosome transport Source: MGI
  6. intracellular protein transport Source: MGI
  7. positive regulation of NK T cell differentiation Source: MGI
  8. protein localization to membrane Source: MGI
  9. protein localization to organelle Source: MGI
  10. regulation of sequestering of zinc ion Source: BHF-UCL
  11. synaptic vesicle membrane organization Source: MGI
  12. vesicle-mediated transport Source: MGI
  13. zinc II ion transport Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-3 complex subunit delta-1
Alternative name(s):
AP-3 complex subunit delta
Adaptor-related protein complex 3 subunit delta-1
Delta-adaptin
Short name:
mBLVR1
Gene namesi
Name:Ap3d1
Synonyms:Ap3d
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:107734. Ap3d1.

Subcellular locationi

  1. Cytoplasm By similarity
  2. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  1. axon Source: MGI
  2. endosome membrane Source: UniProtKB
  3. Golgi membrane Source: UniProtKB-SubCell
  4. lysosomal membrane Source: MGI
  5. membrane Source: MGI
  6. membrane coat Source: InterPro
  7. nucleoplasm Source: MGI
  8. terminal bouton Source: MGI
  9. trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 11991198AP-3 complex subunit delta-1PRO_0000193767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei632 – 6321Phosphoserine2 Publications
Modified residuei634 – 6341Phosphoserine2 Publications
Modified residuei636 – 6361Phosphoserine2 Publications
Modified residuei688 – 6881PhosphoserineBy similarity
Modified residuei754 – 7541PhosphoserineBy similarity
Modified residuei755 – 7551Phosphoserine2 Publications
Modified residuei758 – 7581Phosphothreonine3 Publications
Modified residuei760 – 7601Phosphoserine4 Publications
Modified residuei784 – 7841Phosphoserine2 Publications
Modified residuei825 – 8251Phosphoserine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO54774.
PaxDbiO54774.
PRIDEiO54774.

PTM databases

PhosphoSiteiO54774.

Expressioni

Gene expression databases

BgeeiO54774.
ExpressionAtlasiO54774. baseline and differential.
GenevestigatoriO54774.

Interactioni

Subunit structurei

Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2) (By similarity). AP-3 associates with the BLOC-1 complex.By similarity

Protein-protein interaction databases

BioGridi198134. 6 interactions.
DIPiDIP-32163N.
IntActiO54774. 4 interactions.
MINTiMINT-4104952.

Structurei

3D structure databases

ProteinModelPortaliO54774.
SMRiO54774. Positions 18-595, 697-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati34 – 7138HEAT 1Add
BLAST
Repeati142 – 17938HEAT 2Add
BLAST
Repeati180 – 21637HEAT 3Add
BLAST
Repeati218 – 25437HEAT 4Add
BLAST
Repeati257 – 29640HEAT 5Add
BLAST
Repeati298 – 33639HEAT 6Add
BLAST
Repeati337 – 37337HEAT 7Add
BLAST
Repeati375 – 40935HEAT 8Add
BLAST
Repeati521 – 55838HEAT 9Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili659 – 67921Sequence AnalysisAdd
BLAST
Coiled coili722 – 75029Sequence AnalysisAdd
BLAST
Coiled coili843 – 86321Sequence AnalysisAdd
BLAST
Coiled coili911 – 93424Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi836 – 944109Lys-richAdd
BLAST
Compositional biasi956 – 9605Poly-Glu

Sequence similaritiesi

Contains 9 HEAT repeats.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiCOG5096.
GeneTreeiENSGT00550000075067.
HOGENOMiHOG000000981.
HOVERGENiHBG050520.
InParanoidiO54774.
KOiK12396.
OMAiEDLDFWL.
OrthoDBiEOG79SDWG.
PhylomeDBiO54774.
TreeFamiTF105666.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR010474. BLV_receptor.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF06375. BLVR. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKMVKGSI DRMFDKNLQD LVRGIRNHKE DEAKYISQCI DEIKQELKQD
60 70 80 90 100
NIAVKANAVC KLTYLQMLGY DISWAAFNII EVMSASKFTF KRVGYLAASQ
110 120 130 140 150
CFHEGTDVIM LTTNQIRKDL SSPSQYDTGV ALTGLSCFVT PDLARDLAND
160 170 180 190 200
IMTLMSHTKP YIRKKAVLIM YKVFLKYPES LRPAFPRLKE KLEDPDPGVQ
210 220 230 240 250
SAAVNVICEL ARRNPKNYLS LAPLFFKLMT SSTNNWVLIK IIKLFGALTP
260 270 280 290 300
LEPRLGKKLI EPLTNLIHST SAMSLLYECV NTVIAVLISL SSGMPNHSAS
310 320 330 340 350
IQLCVQKLRI LIEDSDQNLK YLGLLAMSKI LKTHPKSVQS HKDLILQCLD
360 370 380 390 400
DKDESIRLRA LDLLYGMVSK KNLMEIVKKL MTHVDKAEGT TYRDELLTKI
410 420 430 440 450
IDICSQSNYQ HITNFEWYIS ILVELTRLEG TRHGHLIAAQ MLDVAIRVKA
460 470 480 490 500
IRKFAVSQMS SLLDSAHLVA SSTQRNGICE VLYAAAWICG EFSEHLQGPQ
510 520 530 540 550
QTLEAMLRPK VTTLPGHIQA VYVQNVVKLY ASILQQKEQA ADTEAAQEVT
560 570 580 590 600
QLLVERLPQF VQSADLEVQE RASCILQLVK HVQKLQAKGV PVAEEVSALF
610 620 630 640 650
AGELNPVAPK AQKKVPVPEG LDLDAWINEP PSDSESEDEK PKAIFHEEEP
660 670 680 690 700
RHTRRRQPEE DEEELARRRE ARKQEQANNP FYIKSSPSPQ KRYQDAPGVE
710 720 730 740 750
HIPVVQIDLS VPLKVPGMPM SDQYVKLEEQ RRHRQRLEKD KKRKKKEKGK
760 770 780 790 800
RRHSSLPTES DEDIAPAQRV DIITEEMPEN ALPSDEDDKD PNDPYRALDI
810 820 830 840 850
DLDKPLADSE KLPVQKHRNA EAVKSPEKEG VLGVEKKSKK PKKKEKKTKE
860 870 880 890 900
REREKKDKKG EDLDFWLSTT PPPAAAPIPA PSTEELAAST ITSPKDECEV
910 920 930 940 950
LKGEEEDHVD HDQERKSSRH KKKKHRKEKE KEERPRDKKK AKKKQVAPLE
960 970 980 990 1000
NGAAAEEEEE PIPPMSSYCL LAESPYIKVT YDIQASLQKD SQVTVSIILE
1010 1020 1030 1040 1050
NQSSSFLKNM ELNVLDSLNT KMTRPEGSSV HDGVPVPFQL PPGVSNEAQF
1060 1070 1080 1090 1100
VFTIQSIVMA QKLKGTLSFI AKDDEGATHE KLDFRLHFSC SSYLITTPCY
1110 1120 1130 1140 1150
SDAFAKLLES GDLSMNSIKV DGISMSFQNL LAKICFYHHF SVVERVDSCA
1160 1170 1180 1190
SMYSRSIQGH HVCLLVKKGE SSVSVDGKCS DATLLSSLLE EMKTTLAQC
Length:1,199
Mass (Da):135,081
Last modified:June 1, 1998 - v1
Checksum:i29D2D036B36C3B05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004305 mRNA. Translation: BAA24578.1.
BC048786 mRNA. Translation: AAH48786.1.
BC053066 mRNA. Translation: AAH53066.1.
CCDSiCCDS35984.1.
PIRiT13946.
RefSeqiNP_031486.1. NM_007460.1.
UniGeneiMm.209294.

Genome annotation databases

EnsembliENSMUST00000020420; ENSMUSP00000020420; ENSMUSG00000020198.
GeneIDi11776.
KEGGimmu:11776.
UCSCiuc007gek.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004305 mRNA. Translation: BAA24578.1.
BC048786 mRNA. Translation: AAH48786.1.
BC053066 mRNA. Translation: AAH53066.1.
CCDSiCCDS35984.1.
PIRiT13946.
RefSeqiNP_031486.1. NM_007460.1.
UniGeneiMm.209294.

3D structure databases

ProteinModelPortaliO54774.
SMRiO54774. Positions 18-595, 697-729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198134. 6 interactions.
DIPiDIP-32163N.
IntActiO54774. 4 interactions.
MINTiMINT-4104952.

PTM databases

PhosphoSiteiO54774.

Proteomic databases

MaxQBiO54774.
PaxDbiO54774.
PRIDEiO54774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020420; ENSMUSP00000020420; ENSMUSG00000020198.
GeneIDi11776.
KEGGimmu:11776.
UCSCiuc007gek.1. mouse.

Organism-specific databases

CTDi8943.
MGIiMGI:107734. Ap3d1.

Phylogenomic databases

eggNOGiCOG5096.
GeneTreeiENSGT00550000075067.
HOGENOMiHOG000000981.
HOVERGENiHBG050520.
InParanoidiO54774.
KOiK12396.
OMAiEDLDFWL.
OrthoDBiEOG79SDWG.
PhylomeDBiO54774.
TreeFamiTF105666.

Miscellaneous databases

ChiTaRSiAp3d1. mouse.
NextBioi279571.
PROiO54774.
SOURCEiSearch...

Gene expression databases

BgeeiO54774.
ExpressionAtlasiO54774. baseline and differential.
GenevestigatoriO54774.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR010474. BLV_receptor.
IPR002553. Clathrin/coatomer_adapt-like_N.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF06375. BLVR. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse homolog of the bovine leukemia virus receptor is closely related to the delta subunit of adaptor-related protein complex AP-3, not associated with the cell surface."
    Suzuki T., Ikeda H.
    J. Virol. 72:593-599(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Eye.
  3. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632; SER-634; SER-636; SER-755; THR-758; SER-760; SER-784 AND SER-825, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632; SER-634; SER-636; SER-755; THR-758; SER-760 AND SER-784, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-758 AND SER-760, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. "The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse."
    Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.
    Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.

Entry informationi

Entry nameiAP3D1_MOUSE
AccessioniPrimary (citable) accession number: O54774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 1998
Last modified: April 29, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.