AP-3 complex subunit delta-1 - Mus musculus (Mouse)

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O54774 (AP3D1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
AP-3 complex subunit delta-1

Alternative name(s):
AP-3 complex subunit delta
Adapter-related protein complex 3 subunit delta-1
Delta-adaptin
Short name=mBLVR1

Gene names

Name:	Ap3d1
Synonyms:	Ap3d

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1199 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes By similarity. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. Ref.10
Subunit structure	Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2) By similarity. AP-3 associates with the BLOC-1 complex.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the adaptor complexes large subunit family. Contains 9 HEAT repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Domain	Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anterograde axon cargo transport Inferred from mutant phenotype Ref.10. Source: UniProtKB anterograde synaptic vesicle transport Inferred from mutant phenotype Ref.10. Source: UniProtKB antigen processing and presentation, exogenous lipid antigen via MHC class Ib Inferred from mutant phenotype PubMed 14557411. Source: MGI intracellular protein transport Inferred from mutant phenotype PubMed 14557411. Source: MGI positive regulation of NK T cell differentiation Inferred from mutant phenotype PubMed 14557411. Source: MGI protein localization to organelle Inferred from mutant phenotype PubMed 15860731. Source: MGI regulation of sequestering of zinc ion Traceable author statement PubMed 17349999. Source: BHF-UCL synaptic vesicle membrane organization Inferred from mutant phenotype PubMed 15860731. Source: MGI zinc ion transport Traceable author statement PubMed 17349999. Source: BHF-UCL
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endosome membrane Inferred from direct assay PubMed 16162817. Source: UniProtKB membrane coat Inferred from electronic annotation. Source: InterPro terminal bouton Inferred from direct assay PubMed 20089890. Source: MGI trans-Golgi network Traceable author statement PubMed 9714600. Source: MGI
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1199

1199

AP-3 complex subunit delta-1

PRO_0000193767

Regions

Repeat

34 – 71

HEAT 1

Repeat

142 – 179

HEAT 2

Repeat

180 – 216

HEAT 3

Repeat

218 – 254

HEAT 4

Repeat

257 – 296

HEAT 5

Repeat

298 – 336

HEAT 6

Repeat

337 – 373

HEAT 7

Repeat

375 – 409

HEAT 8

Repeat

521 – 558

HEAT 9

Coiled coil

659 – 679

Potential

Coiled coil

722 – 750

Potential

Coiled coil

843 – 863

Potential

Coiled coil

911 – 934

Potential

Compositional bias

836 – 944

109

Lys-rich

Compositional bias

956 – 960

Poly-Glu

Amino acid modifications

Modified residue

632

Phosphoserine Ref.8

Modified residue

634

Phosphoserine Ref.8

Modified residue

636

Phosphoserine Ref.8

Modified residue

688

Phosphoserine By similarity

Modified residue

754

Phosphoserine Ref.7

Modified residue

755

Phosphoserine Ref.5

Modified residue

758

Phosphothreonine Ref.5 Ref.7 Ref.9

Modified residue

760

Phosphoserine Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9

Modified residue

784

Phosphoserine Ref.4 Ref.5 Ref.7 Ref.8

Modified residue

825

Phosphoserine Ref.3

Sequences

Sequence

Length

Mass (Da)

Tools

O54774 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 29D2D036B36C3B05
Show »

FASTA

1,199

135,081

        10         20         30         40         50         60 
MALKMVKGSI DRMFDKNLQD LVRGIRNHKE DEAKYISQCI DEIKQELKQD NIAVKANAVC 

        70         80         90        100        110        120 
KLTYLQMLGY DISWAAFNII EVMSASKFTF KRVGYLAASQ CFHEGTDVIM LTTNQIRKDL 

       130        140        150        160        170        180 
SSPSQYDTGV ALTGLSCFVT PDLARDLAND IMTLMSHTKP YIRKKAVLIM YKVFLKYPES 

       190        200        210        220        230        240 
LRPAFPRLKE KLEDPDPGVQ SAAVNVICEL ARRNPKNYLS LAPLFFKLMT SSTNNWVLIK 

       250        260        270        280        290        300 
IIKLFGALTP LEPRLGKKLI EPLTNLIHST SAMSLLYECV NTVIAVLISL SSGMPNHSAS 

       310        320        330        340        350        360 
IQLCVQKLRI LIEDSDQNLK YLGLLAMSKI LKTHPKSVQS HKDLILQCLD DKDESIRLRA 

       370        380        390        400        410        420 
LDLLYGMVSK KNLMEIVKKL MTHVDKAEGT TYRDELLTKI IDICSQSNYQ HITNFEWYIS 

       430        440        450        460        470        480 
ILVELTRLEG TRHGHLIAAQ MLDVAIRVKA IRKFAVSQMS SLLDSAHLVA SSTQRNGICE 

       490        500        510        520        530        540 
VLYAAAWICG EFSEHLQGPQ QTLEAMLRPK VTTLPGHIQA VYVQNVVKLY ASILQQKEQA 

       550        560        570        580        590        600 
ADTEAAQEVT QLLVERLPQF VQSADLEVQE RASCILQLVK HVQKLQAKGV PVAEEVSALF 

       610        620        630        640        650        660 
AGELNPVAPK AQKKVPVPEG LDLDAWINEP PSDSESEDEK PKAIFHEEEP RHTRRRQPEE 

       670        680        690        700        710        720 
DEEELARRRE ARKQEQANNP FYIKSSPSPQ KRYQDAPGVE HIPVVQIDLS VPLKVPGMPM 

       730        740        750        760        770        780 
SDQYVKLEEQ RRHRQRLEKD KKRKKKEKGK RRHSSLPTES DEDIAPAQRV DIITEEMPEN 

       790        800        810        820        830        840 
ALPSDEDDKD PNDPYRALDI DLDKPLADSE KLPVQKHRNA EAVKSPEKEG VLGVEKKSKK 

       850        860        870        880        890        900 
PKKKEKKTKE REREKKDKKG EDLDFWLSTT PPPAAAPIPA PSTEELAAST ITSPKDECEV 

       910        920        930        940        950        960 
LKGEEEDHVD HDQERKSSRH KKKKHRKEKE KEERPRDKKK AKKKQVAPLE NGAAAEEEEE 

       970        980        990       1000       1010       1020 
PIPPMSSYCL LAESPYIKVT YDIQASLQKD SQVTVSIILE NQSSSFLKNM ELNVLDSLNT 

      1030       1040       1050       1060       1070       1080 
KMTRPEGSSV HDGVPVPFQL PPGVSNEAQF VFTIQSIVMA QKLKGTLSFI AKDDEGATHE 

      1090       1100       1110       1120       1130       1140 
KLDFRLHFSC SSYLITTPCY SDAFAKLLES GDLSMNSIKV DGISMSFQNL LAKICFYHHF 

      1150       1160       1170       1180       1190 
SVVERVDSCA SMYSRSIQGH HVCLLVKKGE SSVSVDGKCS DATLLSSLLE EMKTTLAQC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse homolog of the bovine leukemia virus receptor is closely related to the delta subunit of adaptor-related protein complex AP-3, not associated with the cell surface." Suzuki T., Ikeda H. J. Virol. 72:593-599(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain and Eye.
[3]	"Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760 AND SER-825, MASS SPECTROMETRY. Tissue: Brain.
[4]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760 AND SER-784, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-755; THR-758; SER-760 AND SER-784, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-760, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-754; THR-758; SER-760 AND SER-784, MASS SPECTROMETRY. Tissue: Melanoma.
[8]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-632; SER-634; SER-636; SER-760 AND SER-784, MASS SPECTROMETRY. Tissue: Macrophage.
[9]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-758 AND SER-760, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
[10]	"The schizophrenia susceptibility factor dysbindin and its associated complex sort cargoes from cell bodies to the synapse." Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B., Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V. Mol. Biol. Cell 22:4854-4867(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ASSOCIATION WITH THE BLOC-1 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB004305 mRNA. Translation: BAA24578.1. BC048786 mRNA. Translation: AAH48786.1. BC053066 mRNA. Translation: AAH53066.1.
IPI	IPI00117811.
PIR	T13946.
RefSeq	NP_031486.1. NM_007460.1.
UniGene	Mm.209294.
3D structure databases
ProteinModelPortal	O54774.
SMR	O54774. Positions 18-595, 697-729.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-32163N.
IntAct	O54774. 1 interaction.
PTM databases
PhosphoSite	O54774.
Proteomic databases
PaxDb	O54774.
PRIDE	O54774.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000020420; ENSMUSP00000020420; ENSMUSG00000020198.
GeneID	11776.
KEGG	mmu:11776.
UCSC	uc007gek.1. mouse.
Organism-specific databases
CTD	8943.
MGI	MGI:107734. Ap3d1.
Phylogenomic databases
eggNOG	COG5096.
GeneTree	ENSGT00550000075067.
HOGENOM	HOG000000981.
HOVERGEN	HBG050520.
InParanoid	O54774.
KO	K12396.
OMA	VCKLTYL.
OrthoDB	EOG45TCMD.
Gene expression databases
ArrayExpress	O54774.
Bgee	O54774.
Genevestigator	O54774.
GermOnline	ENSMUSG00000020198. Mus musculus.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR017105. AP3_complex_dsu. IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR010474. BLV_receptor. IPR002553. Clathrin/coatomer_adapt-like_N. [Graphical view]
Pfam	PF01602. Adaptin_N. 1 hit. PF06375. BLVR. 1 hit. [Graphical view]
PIRSF	PIRSF037092. AP3_complex_delta. 1 hit.
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50077. HEAT_REPEAT. False negative. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AP3D1. mouse.
NextBio	279571.
SOURCE	Search...

Entry information

Entry name

AP3D1_MOUSE

Accession

Primary (citable) accession number: O54774

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents