Reviewed, UniProtKB/Swiss-Prot O54753 (H17B6_RAT)

Names and origin

Protein names

Recommended name:
    Hydroxysteroid 17-beta dehydrogenase 6
    EC=1.1.1.62
    EC=1.1.1.63
    EC=1.1.1.105
Alternative name(s):
    17-beta-HSD6
    Oxidative 3-alpha hydroxysteroid dehydrogenase
    3-alpha->beta-hydroxysteroid epimerase
      Short name=3-alpha->beta-HSE

Gene names

Name:	Hsd17b6
Synonyms:	Hsd17b9

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro) By similarity. Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro).
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H. Testosterone + NAD(+) = androst-4-ene-3,17-dione + NADH. Retinol + NAD(+) = retinal + NADH.
Enzyme regulation	Competitively inhibited by 9-cis-retinoic ancid and 13-cis-retinoic acid.
Subcellular location	Microsome membrane; Peripheral membrane protein; Lumenal sideBy similarity. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal sideBy similarity.
Tissue specificity	Detected in prostate, liver and kidney.
Induction	Up-regulated by testosterone. Levels are very low in castrated male rats.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Biophysicochemical properties	Kinetic parameters: Vmax was measured using transfected cell lysates. KM=0.1 µM for 5-alpha-androstan-3-alpha,17-beta-diol KM=0.2 µM for androsterone KM=0.5 µM for dihydrotestosterone KM=1.1 µM for testosterone KM=0.8 µM for estradiol KM=3 µM for NAD KM=1 mM for NADP Vmax=2.5 nmol/min/mg enzyme for 5-alpha-androstan-3-alpha,17-beta-diol Vmax=0.1 nmol/min/mg enzyme for androsterone Vmax=0.5 nmol/min/mg enzyme for dihydrotestosterone Vmax=1.1 nmol/min/mg enzyme for testosterone Vmax=2.1 nmol/min/mg enzyme for estradiol

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Signal
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC retinol dehydrogenase activity Inferred from electronic annotation. Source: EC testosterone 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 317	300	Hydroxysteroid 17-beta dehydrogenase 6		PRO_0000303213
Regions
	Nucleotide binding	33 – 57	25	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	164	1	Substrate Potential
Amino acid modifications
	Glycosylation	161	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	219	1	I → S in AAB88253. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O54753-1 [UniParc]. Last modified September 11, 2007. Version 2. Checksum: 4AB3F3160C6675A9	FASTA	317	36,145
	10 20 30 40 50 60 MWFYLVTLVG LYYLLRWYRE RQVVSHLHDK YVFITGCDSG FGNLLARQLD RRGMRVLAAC 70 80 90 100 110 120 LTEKGAEELK SKTSDRLETV ILDVTNTDSI SAATQWVKEH VGDKGLWGLV NNAGVFQAFA 130 140 150 160 170 180 YIEWCRPEDC MSIFQVNLIG LAQVTLSMLF LVKKARGRIV NVSSVLGRVA LFGGFYSCSK 190 200 210 220 230 240 YGVEAFSDVL RREIRDFGVK VSIIEPGSFK TRMTDAELII EKTKKTWEAT PEHIRESYGQ 250 260 270 280 290 300 QFFDDFCNTT RRELKKCSTN LSLVTDCMEH ALTSKYPRTR YSAGWDARLF FIPLSYLPTS 310 LVDCLLAISR RKPAQAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenases from rat and human prostate." Biswas M.G., Russell D.W. J. Biol. Chem. 272:15959-15966(1997) [PubMed: 9188497] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, INDUCTION, TISSUE SPECIFICITY. Tissue: Prostate.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U89280 mRNA. Translation: AAB88253.1. Different initiation. BC088104 mRNA. Translation: AAH88104.1. Different initiation. BC091114 mRNA. Translation: AAH91114.1. Different initiation.
RefSeq	NP_775427.1.
UniGene	Rn.10857
3D structure databases
HSSP	HSSP built from PDB template 1FDS based on UniProtKB P14061.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000007758. Rattus norvegicus. [Contig view]
GeneID	286964.
KEGG	rno:286964.
Organism-specific databases
RGD	708343. Hsd17b6.
Phylogenomic databases
HOVERGEN	O54753.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	625175.

Entry information

Entry name

H17B6_RAT

Accession

Primary (citable) accession number: O54753
Secondary accession number(s): Q5M8C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	November 25, 2008
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)