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O54753

- H17B6_RAT

UniProt

O54753 - H17B6_RAT

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Protein

17-beta-hydroxysteroid dehydrogenase type 6

Gene

Hsd17b6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro) (By similarity). Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro).By similarity1 Publication

Catalytic activityi

17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
Testosterone + NAD+ = androstenedione + NADH.
All-trans-retinol-[cellular-retinol-binding-protein] + NAD+ = all-trans-retinal-[cellular-retinol-binding-protein] + NADH.

Enzyme regulationi

Competitively inhibited by 9-cis-retinoic ancid and 13-cis-retinoic acid.1 Publication

Kineticsi

Vmax was measured using transfected cell lysates.

  1. KM=0.1 µM for 5-alpha-androstan-3-alpha,17-beta-diol1 Publication
  2. KM=0.2 µM for androsterone1 Publication
  3. KM=0.5 µM for dihydrotestosterone1 Publication
  4. KM=1.1 µM for testosterone1 Publication
  5. KM=0.8 µM for estradiol1 Publication
  6. KM=3 µM for NAD1 Publication
  7. KM=1 mM for NADP1 Publication

Vmax=2.5 nmol/min/mg enzyme for 5-alpha-androstan-3-alpha,17-beta-diol1 Publication

Vmax=0.1 nmol/min/mg enzyme for androsterone1 Publication

Vmax=0.5 nmol/min/mg enzyme for dihydrotestosterone1 Publication

Vmax=1.1 nmol/min/mg enzyme for testosterone1 Publication

Vmax=2.1 nmol/min/mg enzyme for estradiol1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei164 – 1641SubstrateSequence Analysis
Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi33 – 5725NADBy similarityAdd
BLAST

GO - Molecular functioni

  1. estradiol 17-beta-dehydrogenase activity Source: UniProtKB-EC
  2. retinol dehydrogenase activity Source: UniProtKB-EC
  3. testosterone dehydrogenase (NAD+) activity Source: RGD

GO - Biological processi

  1. androgen metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
17-beta-hydroxysteroid dehydrogenase type 6 (EC:1.1.1.105, EC:1.1.1.239, EC:1.1.1.62)
Short name:
17-beta-HSD 6
Short name:
17-beta-HSD6
Alternative name(s):
3-alpha->beta-hydroxysteroid epimerase
Short name:
3-alpha->beta-HSE
Oxidative 3-alpha hydroxysteroid dehydrogenase
Gene namesi
Name:Hsd17b6
Synonyms:Hsd17b9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi708343. Hsd17b6.

Subcellular locationi

Microsome membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity. Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 31730017-beta-hydroxysteroid dehydrogenase type 6PRO_0000303213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54753.
PRIDEiO54753.

Expressioni

Tissue specificityi

Detected in prostate, liver and kidney.1 Publication

Inductioni

Up-regulated by testosterone. Levels are very low in castrated male rats.1 Publication

Gene expression databases

GenevestigatoriO54753.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031599.

Structurei

3D structure databases

ProteinModelPortaliO54753.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1028.
HOVERGENiHBG005482.
InParanoidiO54753.
KOiK13369.
PhylomeDBiO54753.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54753-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWFYLVTLVG LYYLLRWYRE RQVVSHLHDK YVFITGCDSG FGNLLARQLD
60 70 80 90 100
RRGMRVLAAC LTEKGAEELK SKTSDRLETV ILDVTNTDSI SAATQWVKEH
110 120 130 140 150
VGDKGLWGLV NNAGVFQAFA YIEWCRPEDC MSIFQVNLIG LAQVTLSMLF
160 170 180 190 200
LVKKARGRIV NVSSVLGRVA LFGGFYSCSK YGVEAFSDVL RREIRDFGVK
210 220 230 240 250
VSIIEPGSFK TRMTDAELII EKTKKTWEAT PEHIRESYGQ QFFDDFCNTT
260 270 280 290 300
RRELKKCSTN LSLVTDCMEH ALTSKYPRTR YSAGWDARLF FIPLSYLPTS
310
LVDCLLAISR RKPAQAV
Length:317
Mass (Da):36,145
Last modified:September 11, 2007 - v2
Checksum:i4AB3F3160C6675A9
GO

Sequence cautioni

The sequence AAB88253.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH88104.1 differs from that shown. Reason: Erroneous initiation.
The sequence AAH91114.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191I → S in AAB88253. (PubMed:9188497)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U89280 mRNA. Translation: AAB88253.1. Different initiation.
BC088104 mRNA. Translation: AAH88104.1. Different initiation.
BC091114 mRNA. Translation: AAH91114.1. Different initiation.
RefSeqiNP_775427.1. NM_173305.1.
UniGeneiRn.10857.

Genome annotation databases

GeneIDi286964.
KEGGirno:286964.
UCSCiRGD:708343. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U89280 mRNA. Translation: AAB88253.1 . Different initiation.
BC088104 mRNA. Translation: AAH88104.1 . Different initiation.
BC091114 mRNA. Translation: AAH91114.1 . Different initiation.
RefSeqi NP_775427.1. NM_173305.1.
UniGenei Rn.10857.

3D structure databases

ProteinModelPortali O54753.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000031599.

Proteomic databases

PaxDbi O54753.
PRIDEi O54753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 286964.
KEGGi rno:286964.
UCSCi RGD:708343. rat.

Organism-specific databases

CTDi 8630.
RGDi 708343. Hsd17b6.

Phylogenomic databases

eggNOGi COG1028.
HOVERGENi HBG005482.
InParanoidi O54753.
KOi K13369.
PhylomeDBi O54753.

Miscellaneous databases

NextBioi 625175.

Gene expression databases

Genevestigatori O54753.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenases from rat and human prostate."
    Biswas M.G., Russell D.W.
    J. Biol. Chem. 272:15959-15966(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, INDUCTION, TISSUE SPECIFICITY.
    Tissue: Prostate.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiH17B6_RAT
AccessioniPrimary (citable) accession number: O54753
Secondary accession number(s): Q5M8C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3