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O54753

- H17B6_RAT

UniProt

O54753 - H17B6_RAT

Protein

17-beta-hydroxysteroid dehydrogenase type 6

Gene

Hsd17b6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    NAD-dependent oxidoreductase with broad substrate specificity that shows both oxidative and reductive activity (in vitro). Has retinol dehydrogenase activity towards all-trans-retinol (in vitro) By similarity. Has 17-beta-hydroxysteroid dehydrogenase activity towards various steroids (in vitro). Converts 5-alpha-androstan-3-alpha,17-beta-diol to androsterone and estradiol to estrone (in vitro). Has 3-alpha-hydroxysteroid dehydrogenase activity towards androsterone (in vitro).By similarity1 Publication

    Catalytic activityi

    17-beta-estradiol + NAD(P)+ = estrone + NAD(P)H.
    Testosterone + NAD+ = androstenedione + NADH.
    All-trans-retinol-[cellular-retinol-binding-protein] + NAD+ = all-trans-retinal-[cellular-retinol-binding-protein] + NADH.

    Enzyme regulationi

    Competitively inhibited by 9-cis-retinoic ancid and 13-cis-retinoic acid.1 Publication

    Kineticsi

    Vmax was measured using transfected cell lysates.

    1. KM=0.1 µM for 5-alpha-androstan-3-alpha,17-beta-diol1 Publication
    2. KM=0.2 µM for androsterone1 Publication
    3. KM=0.5 µM for dihydrotestosterone1 Publication
    4. KM=1.1 µM for testosterone1 Publication
    5. KM=0.8 µM for estradiol1 Publication
    6. KM=3 µM for NAD1 Publication
    7. KM=1 mM for NADP1 Publication

    Vmax=2.5 nmol/min/mg enzyme for 5-alpha-androstan-3-alpha,17-beta-diol1 Publication

    Vmax=0.1 nmol/min/mg enzyme for androsterone1 Publication

    Vmax=0.5 nmol/min/mg enzyme for dihydrotestosterone1 Publication

    Vmax=1.1 nmol/min/mg enzyme for testosterone1 Publication

    Vmax=2.1 nmol/min/mg enzyme for estradiol1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei164 – 1641SubstrateSequence Analysis
    Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 5725NADBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. estradiol 17-beta-dehydrogenase activity Source: UniProtKB-EC
    2. retinol dehydrogenase activity Source: UniProtKB-EC
    3. testosterone dehydrogenase (NAD+) activity Source: RGD

    GO - Biological processi

    1. androgen metabolic process Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid metabolism, Steroid metabolism

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    17-beta-hydroxysteroid dehydrogenase type 6 (EC:1.1.1.105, EC:1.1.1.239, EC:1.1.1.62)
    Short name:
    17-beta-HSD 6
    Short name:
    17-beta-HSD6
    Alternative name(s):
    3-alpha->beta-hydroxysteroid epimerase
    Short name:
    3-alpha->beta-HSE
    Oxidative 3-alpha hydroxysteroid dehydrogenase
    Gene namesi
    Name:Hsd17b6
    Synonyms:Hsd17b9
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi708343. Hsd17b6.

    Subcellular locationi

    Microsome membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity. Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 31730017-beta-hydroxysteroid dehydrogenase type 6PRO_0000303213Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO54753.
    PRIDEiO54753.

    Expressioni

    Tissue specificityi

    Detected in prostate, liver and kidney.1 Publication

    Inductioni

    Up-regulated by testosterone. Levels are very low in castrated male rats.1 Publication

    Gene expression databases

    GenevestigatoriO54753.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000031599.

    Structurei

    3D structure databases

    ProteinModelPortaliO54753.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1028.
    HOVERGENiHBG005482.
    InParanoidiO54753.
    KOiK13369.
    PhylomeDBiO54753.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O54753-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWFYLVTLVG LYYLLRWYRE RQVVSHLHDK YVFITGCDSG FGNLLARQLD    50
    RRGMRVLAAC LTEKGAEELK SKTSDRLETV ILDVTNTDSI SAATQWVKEH 100
    VGDKGLWGLV NNAGVFQAFA YIEWCRPEDC MSIFQVNLIG LAQVTLSMLF 150
    LVKKARGRIV NVSSVLGRVA LFGGFYSCSK YGVEAFSDVL RREIRDFGVK 200
    VSIIEPGSFK TRMTDAELII EKTKKTWEAT PEHIRESYGQ QFFDDFCNTT 250
    RRELKKCSTN LSLVTDCMEH ALTSKYPRTR YSAGWDARLF FIPLSYLPTS 300
    LVDCLLAISR RKPAQAV 317
    Length:317
    Mass (Da):36,145
    Last modified:September 11, 2007 - v2
    Checksum:i4AB3F3160C6675A9
    GO

    Sequence cautioni

    The sequence AAB88253.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH88104.1 differs from that shown. Reason: Erroneous initiation.
    The sequence AAH91114.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2191I → S in AAB88253. (PubMed:9188497)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89280 mRNA. Translation: AAB88253.1. Different initiation.
    BC088104 mRNA. Translation: AAH88104.1. Different initiation.
    BC091114 mRNA. Translation: AAH91114.1. Different initiation.
    RefSeqiNP_775427.1. NM_173305.1.
    UniGeneiRn.10857.

    Genome annotation databases

    GeneIDi286964.
    KEGGirno:286964.
    UCSCiRGD:708343. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U89280 mRNA. Translation: AAB88253.1 . Different initiation.
    BC088104 mRNA. Translation: AAH88104.1 . Different initiation.
    BC091114 mRNA. Translation: AAH91114.1 . Different initiation.
    RefSeqi NP_775427.1. NM_173305.1.
    UniGenei Rn.10857.

    3D structure databases

    ProteinModelPortali O54753.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000031599.

    Proteomic databases

    PaxDbi O54753.
    PRIDEi O54753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 286964.
    KEGGi rno:286964.
    UCSCi RGD:708343. rat.

    Organism-specific databases

    CTDi 8630.
    RGDi 708343. Hsd17b6.

    Phylogenomic databases

    eggNOGi COG1028.
    HOVERGENi HBG005482.
    InParanoidi O54753.
    KOi K13369.
    PhylomeDBi O54753.

    Miscellaneous databases

    NextBioi 625175.

    Gene expression databases

    Genevestigatori O54753.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenases from rat and human prostate."
      Biswas M.G., Russell D.W.
      J. Biol. Chem. 272:15959-15966(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, INDUCTION, TISSUE SPECIFICITY.
      Tissue: Prostate.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiH17B6_RAT
    AccessioniPrimary (citable) accession number: O54753
    Secondary accession number(s): Q5M8C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3