ID   CRX_MOUSE               Reviewed;         299 AA.
AC   O54751;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   27-MAR-2024, entry version 178.
DE   RecName: Full=Cone-rod homeobox protein;
GN   Name=Crx;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Retina;
RX   PubMed=9390562; DOI=10.1016/s0092-8674(00)80439-0;
RA   Furukawa T., Morrow E.M., Cepko C.L.;
RT   "Crx, a novel otx-like homeobox gene, shows photoreceptor-specific
RT   expression and regulates photoreceptor differentiation.";
RL   Cell 91:531-541(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   FUNCTION AS TRANSCRIPTION ACTIVATOR, SUBCELLULAR LOCATION, DNA-BINDING,
RP   INTERACTION WITH NRL AND RORB, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC   STRAIN=C57BL/6J; TISSUE=Embryonic eye;
RX   PubMed=16574740; DOI=10.1210/me.2005-0505;
RA   Srinivas M., Ng L., Liu H., Jia L., Forrest D.;
RT   "Activation of the blue opsin gene in cone photoreceptor development by
RT   retinoid-related orphan receptor beta.";
RL   Mol. Endocrinol. 20:1728-1741(2006).
CC   -!- FUNCTION: Transcription factor that binds and transactivates the
CC       sequence 5'-TAATC[CA]-3' which is found upstream of several
CC       photoreceptor-specific genes, including the opsin genes. Acts
CC       synergistically with other transcription factors, such as NRL, RORB and
CC       RAX, to regulate photoreceptor cell-specific gene transcription.
CC       Essential for the maintenance of mammalian photoreceptors.
CC       {ECO:0000269|PubMed:16574740}.
CC   -!- SUBUNIT: Interacts (via the homeobox) with NRL (via the leucine-zipper
CC       domain). Interacts with PDC, RAX2, RORB and SCA7.
CC       {ECO:0000269|PubMed:16574740}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000269|PubMed:16574740}.
CC   -!- TISSUE SPECIFICITY: Retina. {ECO:0000269|PubMed:16574740}.
CC   -!- DEVELOPMENTAL STAGE: At 17.5 dpc, expressed in a narrow zone in the
CC       peripheral outer nuclear layer of the retina. At P12, expressed over
CC       the entire photoreceptor layer. {ECO:0000269|PubMed:16574740}.
CC   -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
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DR   EMBL; U77615; AAB88585.1; -; mRNA.
DR   EMBL; BC016502; AAH16502.1; -; mRNA.
DR   CCDS; CCDS20840.1; -.
DR   RefSeq; NP_001106801.1; NM_001113330.1.
DR   RefSeq; NP_031796.1; NM_007770.4.
DR   AlphaFoldDB; O54751; -.
DR   SMR; O54751; -.
DR   BioGRID; 198905; 3.
DR   STRING; 10090.ENSMUSP00000134400; -.
DR   PhosphoSitePlus; O54751; -.
DR   PaxDb; 10090-ENSMUSP00000134400; -.
DR   ProteomicsDB; 285336; -.
DR   Antibodypedia; 31635; 302 antibodies from 33 providers.
DR   DNASU; 12951; -.
DR   Ensembl; ENSMUST00000044434.13; ENSMUSP00000043436.7; ENSMUSG00000041578.16.
DR   GeneID; 12951; -.
DR   KEGG; mmu:12951; -.
DR   UCSC; uc009fgm.2; mouse.
DR   AGR; MGI:1194883; -.
DR   CTD; 1406; -.
DR   MGI; MGI:1194883; Crx.
DR   VEuPathDB; HostDB:ENSMUSG00000041578; -.
DR   eggNOG; KOG2251; Eukaryota.
DR   GeneTree; ENSGT00940000161634; -.
DR   HOGENOM; CLU_064370_0_0_1; -.
DR   InParanoid; O54751; -.
DR   OMA; TWKFAYN; -.
DR   OrthoDB; 2913621at2759; -.
DR   PhylomeDB; O54751; -.
DR   TreeFam; TF351179; -.
DR   BioGRID-ORCS; 12951; 3 hits in 83 CRISPR screens.
DR   ChiTaRS; Crx; mouse.
DR   PRO; PR:O54751; -.
DR   Proteomes; UP000000589; Chromosome 7.
DR   RNAct; O54751; Protein.
DR   Bgee; ENSMUSG00000041578; Expressed in retinal neural layer and 25 other cell types or tissues.
DR   ExpressionAtlas; O54751; baseline and differential.
DR   GO; GO:0000785; C:chromatin; ISS:ARUK-UCL.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISS:ARUK-UCL.
DR   GO; GO:0005667; C:transcription regulator complex; IDA:MGI.
DR   GO; GO:0003682; F:chromatin binding; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; ISO:MGI.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; ISS:ARUK-UCL.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0043522; F:leucine zipper domain binding; ISO:MGI.
DR   GO; GO:0016922; F:nuclear receptor binding; IPI:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:MGI.
DR   GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISS:ARUK-UCL.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IDA:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR   GO; GO:0060041; P:retina development in camera-type eye; IMP:MGI.
DR   GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR013851; Otx_TF_C.
DR   PANTHER; PTHR45793:SF22; CONE-ROD HOMEOBOX PROTEIN; 1.
DR   PANTHER; PTHR45793; HOMEOBOX PROTEIN; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF03529; TF_Otx; 1.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; O54751; MM.
PE   1: Evidence at protein level;
KW   Activator; Developmental protein; Differentiation; DNA-binding; Homeobox;
KW   Neurogenesis; Nucleus; Reference proteome; Sensory transduction;
KW   Transcription; Transcription regulation; Vision.
FT   CHAIN           1..299
FT                   /note="Cone-rod homeobox protein"
FT                   /id="PRO_0000048863"
FT   DNA_BIND        39..98
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          94..155
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          236..258
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        126..155
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        241..258
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   299 AA;  32374 MW;  4FE2C27D8070B524 CRC64;
     MMAYMNPGPH YSVNALALSG PNVDLMHQAV PYSSAPRKQR RERTTFTRSQ LEELEALFAK
     TQYPDVYARE EVALKINLPE SRVQVWFKNR RAKCRQQRQQ QKQQQQPPGA QTKARPAKRK
     AGTSPRPSTD VCTDPLGISD SYSPSLPGPS GSPTTAVATV SIWSPASEAP LPEAQRAGLV
     ASGPSLTSAP YAMTYAPASA FCSSPSAYAS PSSYFSGLDP YLSPMVPQLG GPALSPLSGP
     SVGPSLAQSP TSLSGQSYST YSPVDSLEFK DPTGTWKFTY NPMDPLDYKD QSAWKFQIL
//