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Protein

Cone-rod homeobox protein

Gene

Crx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, such as NRL, RORB and RAX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi39 – 9860HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. leucine zipper domain binding Source: MGI
  3. nuclear hormone receptor binding Source: UniProtKB
  4. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: MGI
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
  6. RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  7. sequence-specific DNA binding transcription factor activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. nervous system development Source: UniProtKB-KW
  3. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  4. regulation of transcription, DNA-templated Source: UniProtKB
  5. response to stimulus Source: UniProtKB-KW
  6. retina development in camera-type eye Source: MGI
  7. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis, Sensory transduction, Transcription, Transcription regulation, Vision

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cone-rod homeobox protein
Gene namesi
Name:Crx
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1194883. Crx.

Subcellular locationi

Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Cone-rod homeobox proteinPRO_0000048863Add
BLAST

Proteomic databases

PRIDEiO54751.

PTM databases

PhosphoSiteiO54751.

Expressioni

Tissue specificityi

Retina.1 Publication

Developmental stagei

At 17.5 dpc, expressed in a narrow zone in the peripheral outer nuclear layer of the retina. At P12, expressed over the entire photoreceptor layer.1 Publication

Gene expression databases

BgeeiO54751.
CleanExiMM_CRX.
ExpressionAtlasiO54751. baseline and differential.
GenevestigatoriO54751.

Interactioni

Subunit structurei

Interacts (via the homeobox) with NRL (via the leucine-zipper domain). Interacts with PDC, RAX2, RORB and SCA7.1 Publication

Protein-protein interaction databases

BioGridi198905. 2 interactions.
STRINGi10090.ENSMUSP00000043436.

Structurei

3D structure databases

ProteinModelPortaliO54751.
SMRiO54751. Positions 34-95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the paired homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG324074.
HOGENOMiHOG000082677.
HOVERGENiHBG004028.
InParanoidiO54751.
KOiK09337.
OrthoDBiEOG74J98P.
PhylomeDBiO54751.
TreeFamiTF351179.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR013851. Otx_TF_C.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03529. TF_Otx. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54751-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAYMNPGPH YSVNALALSG PNVDLMHQAV PYSSAPRKQR RERTTFTRSQ
60 70 80 90 100
LEELEALFAK TQYPDVYARE EVALKINLPE SRVQVWFKNR RAKCRQQRQQ
110 120 130 140 150
QKQQQQPPGA QTKARPAKRK AGTSPRPSTD VCTDPLGISD SYSPSLPGPS
160 170 180 190 200
GSPTTAVATV SIWSPASEAP LPEAQRAGLV ASGPSLTSAP YAMTYAPASA
210 220 230 240 250
FCSSPSAYAS PSSYFSGLDP YLSPMVPQLG GPALSPLSGP SVGPSLAQSP
260 270 280 290
TSLSGQSYST YSPVDSLEFK DPTGTWKFTY NPMDPLDYKD QSAWKFQIL
Length:299
Mass (Da):32,374
Last modified:June 1, 1998 - v1
Checksum:i4FE2C27D8070B524
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77615 mRNA. Translation: AAB88585.1.
BC016502 mRNA. Translation: AAH16502.1.
CCDSiCCDS20840.1.
RefSeqiNP_001106801.1. NM_001113330.1.
NP_031796.1. NM_007770.4.
UniGeneiMm.441911.

Genome annotation databases

EnsembliENSMUST00000044434; ENSMUSP00000043436; ENSMUSG00000041578.
GeneIDi12951.
KEGGimmu:12951.
UCSCiuc009fgm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77615 mRNA. Translation: AAB88585.1.
BC016502 mRNA. Translation: AAH16502.1.
CCDSiCCDS20840.1.
RefSeqiNP_001106801.1. NM_001113330.1.
NP_031796.1. NM_007770.4.
UniGeneiMm.441911.

3D structure databases

ProteinModelPortaliO54751.
SMRiO54751. Positions 34-95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198905. 2 interactions.
STRINGi10090.ENSMUSP00000043436.

PTM databases

PhosphoSiteiO54751.

Proteomic databases

PRIDEiO54751.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044434; ENSMUSP00000043436; ENSMUSG00000041578.
GeneIDi12951.
KEGGimmu:12951.
UCSCiuc009fgm.2. mouse.

Organism-specific databases

CTDi1406.
MGIiMGI:1194883. Crx.

Phylogenomic databases

eggNOGiNOG324074.
HOGENOMiHOG000082677.
HOVERGENiHBG004028.
InParanoidiO54751.
KOiK09337.
OrthoDBiEOG74J98P.
PhylomeDBiO54751.
TreeFamiTF351179.

Miscellaneous databases

NextBioi282658.
PROiO54751.
SOURCEiSearch...

Gene expression databases

BgeeiO54751.
CleanExiMM_CRX.
ExpressionAtlasiO54751. baseline and differential.
GenevestigatoriO54751.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR013851. Otx_TF_C.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03529. TF_Otx. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Crx, a novel otx-like homeobox gene, shows photoreceptor-specific expression and regulates photoreceptor differentiation."
    Furukawa T., Morrow E.M., Cepko C.L.
    Cell 91:531-541(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Activation of the blue opsin gene in cone photoreceptor development by retinoid-related orphan receptor beta."
    Srinivas M., Ng L., Liu H., Jia L., Forrest D.
    Mol. Endocrinol. 20:1728-1741(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS TRANSCRIPTION ACTIVATOR, SUBCELLULAR LOCATION, DNA-BINDING, INTERACTION WITH NRL AND RORB, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: C57BL/6.
    Tissue: Embryonic eye.

Entry informationi

Entry nameiCRX_MOUSE
AccessioniPrimary (citable) accession number: O54751
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 1998
Last modified: March 4, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.