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O54735

- PDE5A_RAT

UniProt

O54735 - PDE5A_RAT

Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei571 – 5711Proton donorBy similarity
    Metal bindingi575 – 5751Divalent metal cation 1By similarity
    Metal bindingi611 – 6111Divalent metal cation 1By similarity
    Metal bindingi612 – 6121Divalent metal cation 1By similarity
    Metal bindingi612 – 6121Divalent metal cation 2By similarity
    Metal bindingi722 – 7221Divalent metal cation 1By similarity
    Binding sitei775 – 7751cGMPBy similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: RGD
    2. cGMP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cGMP catabolic process Source: UniProtKB-UniPathway
    2. nervous system development Source: RGD
    3. positive regulation of apoptotic process Source: RGD
    4. positive regulation of chronic inflammatory response Source: RGD
    5. positive regulation of vasoconstriction Source: RGD
    6. regulation of the force of heart contraction Source: RGD
    7. response to hypoxia Source: RGD
    8. response to lipopolysaccharide Source: RGD
    9. response to testosterone Source: RGD
    10. short-term memory Source: RGD
    11. signal transduction Source: InterPro
    12. vasodilation Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cGMP, cGMP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00763; UER00748.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesterase (EC:3.1.4.35)
    Alternative name(s):
    cGMP-binding cGMP-specific phosphodiesterase
    Short name:
    CGB-PDE
    Gene namesi
    Name:Pde5a
    Synonyms:Pde5
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi620995. Pde5a.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 833833cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000198825Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei60 – 601PhosphoserineSequence Analysis

    Post-translational modificationi

    Phosphorylation is regulated by binding of cGMP to the two allosteric sites. Phosphorylation by PRKG1 leads to its activation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO54735.
    PRIDEiO54735.

    PTM databases

    PhosphoSiteiO54735.

    Expressioni

    Gene expression databases

    GenevestigatoriO54735.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliO54735.
    SMRiO54735. Positions 122-269, 493-818.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini122 – 272151GAF 1Add
    BLAST
    Domaini304 – 461158GAF 2Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni546 – 811266CatalyticBy similarityAdd
    BLAST

    Domaini

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG270709.
    HOGENOMiHOG000007068.
    HOVERGENiHBG101207.
    InParanoidiO54735.
    KOiK13762.
    PhylomeDBiO54735.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 2 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform PDE5A2 (identifier: O54735-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MLPFGDKTRD MVNAWFSERV HNIPVCKEGI RAHTESCSCS LPQSPHADNT    50
    TPGAPARKIS ASEFDRPLRP IVVKDSEGTV SFLSDSGKKE QMPLTSPRFD 100
    SDEGDQCSRL LELVKDISSH LDVTALCHKI FLHIHGLISA DRYSLFLVCE 150
    DSSKDKFLVS RLFDVAEGST LEEASNNCIR LEWNKGIVGH VAAFGEPLNI 200
    KDAYEDPRFN AEVDQITGYK TQSILCMPIK NHREEVVGVA QAINKKSGNG 250
    GTFTEKDEKD FAAYLAFCGI VLHNAQLYET SLLENKRNQV LLDLASLIFE 300
    EQQSLEVILK KIAATIISFM QVQKCTIFIV DEDCPDSFSR VFQMEWEEVG 350
    KSSEPLTREH DANKINYMYA QYVKNTMEPL NIPDVTKDNR FPWTNENMGH 400
    INTHCIRSLL CTPIKNGKKN KVIGVCQLVN KMEEKTGKIK AFNQNDEQFL 450
    EAFVIFCGLG IQNTQMYEAV ERAMAKQMVT LEVLSYHASA AEEETRELQA 500
    LAAAVVPSAQ TLKITDFSFS DFELSDLETA LCTIRMFTDL NLVQNFQMKH 550
    EVLCRWILSV KKNYRKNVAY HNWRHAFNTA QCMFAALKAG KIQNKLTDLE 600
    TLALLIAALS HDLDHRGVNN SYIQRSEHPL AQLYCHSTME HHHFDQCLMV 650
    LNSPGNQILS GLSIEEYKTT LKIIKQAILA TDLALYIKRR GEFFELIRKN 700
    EFSFEDPLQK ELFLAMLMTA CDLSAITKPW PIQQRIAELV AAEFFDQGDR 750
    ERKELNMEPA DLMNREKKNK IPSMQVGFID AICLQLYEAL THVSEDCLPL 800
    LDGCRKNRQK WQALADQQEK TLLNGESGQA KRD 833
    Length:833
    Mass (Da):94,556
    Last modified:June 1, 1998 - v1
    Checksum:i712DC159C80CB09D
    GO
    Isoform PDE5A1 (identifier: O54735-2)

    Sequence is not available
    Length:
    Mass (Da):

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89093 mRNA. Translation: BAA23672.1.
    RefSeqiNP_598268.1. NM_133584.1. [O54735-1]
    UniGeneiRn.10861.
    Rn.133138.

    Genome annotation databases

    GeneIDi171115.
    KEGGirno:171115.
    UCSCiRGD:620995. rat. [O54735-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D89093 mRNA. Translation: BAA23672.1 .
    RefSeqi NP_598268.1. NM_133584.1. [O54735-1 ]
    UniGenei Rn.10861.
    Rn.133138.

    3D structure databases

    ProteinModelPortali O54735.
    SMRi O54735. Positions 122-269, 493-818.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi O54735.
    ChEMBLi CHEMBL4567.

    PTM databases

    PhosphoSitei O54735.

    Proteomic databases

    PaxDbi O54735.
    PRIDEi O54735.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 171115.
    KEGGi rno:171115.
    UCSCi RGD:620995. rat. [O54735-1 ]

    Organism-specific databases

    CTDi 8654.
    RGDi 620995. Pde5a.

    Phylogenomic databases

    eggNOGi NOG270709.
    HOGENOMi HOG000007068.
    HOVERGENi HBG101207.
    InParanoidi O54735.
    KOi K13762.
    PhylomeDBi O54735.

    Enzyme and pathway databases

    UniPathwayi UPA00763 ; UER00748 .

    Miscellaneous databases

    NextBioi 621836.
    PROi O54735.

    Gene expression databases

    Genevestigatori O54735.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 2 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 2 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of rat cGMP-binding cGMP-specific phosphodiesterase mRNA in Purkinje cell layers during postnatal neuronal development."
      Kotera J., Yanaka N., Fujishige K., Imai Y., Akatsuka H., Ishizuka T., Kawashima K., Omori K.
      Eur. J. Biochem. 249:434-442(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Lung.

    Entry informationi

    Entry nameiPDE5A_RAT
    AccessioniPrimary (citable) accession number: O54735
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3