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Protein

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

Gene

Ddost

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.

Catalytic activityi

Dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit (EC:2.4.99.18)
Short name:
DDOST 48 kDa subunit
Short name:
Oligosaccharyl transferase 48 kDa subunit
Gene namesi
Name:Ddost
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1194508. Ddost.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 412384LumenalSequence analysisAdd
BLAST
Transmembranei413 – 43220HelicalSequence analysisAdd
BLAST
Topological domaini433 – 4419CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828By similarityAdd
BLAST
Chaini29 – 441413Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunitPRO_0000021958Add
BLAST

Proteomic databases

EPDiO54734.
MaxQBiO54734.
PaxDbiO54734.
PeptideAtlasiO54734.
PRIDEiO54734.
TopDownProteomicsiO54734.

PTM databases

iPTMnetiO54734.
PhosphoSiteiO54734.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028757.
GenevisibleiO54734. MM.

Interactioni

Subunit structurei

Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome. Also identified as part of a complex which includes CANX, DERL1, DERL2, DDOST/OST48, RPN1, RPN2, SELK, VIMP, STT3A AND VCP. This contains known members of the OST complex and may be a form of this complex (By similarity).By similarity

Protein-protein interaction databases

BioGridi199080. 1 interaction.
IntActiO54734. 7 interactions.
MINTiMINT-4114343.
STRINGi10090.ENSMUSP00000030538.

Structurei

3D structure databases

ProteinModelPortaliO54734.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDOST 48 kDa subunit family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
HOVERGENiHBG053378.
InParanoidiO54734.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG091G07Z5.
TreeFamiTF314821.

Family and domain databases

InterProiIPR005013. DDOST_48_kDa_subunit.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMDPRLAVR AWPLCGLLLA VLGCVCASGP RTLVLLDNLN VRDTHSLFFR
60 70 80 90 100
SLKDRGFELT FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET
110 120 130 140 150
ISAFIDGGGS VLVAASSDIG DPLRELGSEC GIEFDEEKTA VIDHHNYDVS
160 170 180 190 200
DLGQHTLIVA DTENLLKAPT IVGKSSLNPI LFRGVGMVAD PDNPLVLDIL
210 220 230 240 250
TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV IFSGSLDFFS
260 270 280 290 300
DAFFNSAVQK ATPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR
310 320 330 340 350
VGEMAPPNAY TVTDLVEYSI IIEQLSNGKW VPFDGDDIQL EFVRIDPFVR
360 370 380 390 400
TFLKRKGGKY SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH
410 420 430 440
TQYERFIPSA YPYYASAFSM MAGLFIFSIV FLHMKEKEKS D
Length:441
Mass (Da):49,028
Last modified:July 27, 2011 - v2
Checksum:iC1B9A0B94D2A1CAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti321 – 3211I → V in BAA23671 (PubMed:9367678).Curated
Sequence conflicti321 – 3211I → V in BAB82434 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89063 mRNA. Translation: BAA23671.1.
AB012717 Genomic DNA. Translation: BAB82434.1.
AK081542 mRNA. Translation: BAC38250.1.
AL807249 Genomic DNA. Translation: CAM18871.1.
BC068132 mRNA. Translation: AAH68132.1.
CCDSiCCDS18824.1.
RefSeqiNP_031864.2. NM_007838.2.
UniGeneiMm.470295.
Mm.7236.

Genome annotation databases

EnsembliENSMUST00000030538; ENSMUSP00000030538; ENSMUSG00000028757.
GeneIDi13200.
KEGGimmu:13200.
UCSCiuc008vkt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D89063 mRNA. Translation: BAA23671.1.
AB012717 Genomic DNA. Translation: BAB82434.1.
AK081542 mRNA. Translation: BAC38250.1.
AL807249 Genomic DNA. Translation: CAM18871.1.
BC068132 mRNA. Translation: AAH68132.1.
CCDSiCCDS18824.1.
RefSeqiNP_031864.2. NM_007838.2.
UniGeneiMm.470295.
Mm.7236.

3D structure databases

ProteinModelPortaliO54734.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199080. 1 interaction.
IntActiO54734. 7 interactions.
MINTiMINT-4114343.
STRINGi10090.ENSMUSP00000030538.

PTM databases

iPTMnetiO54734.
PhosphoSiteiO54734.

Proteomic databases

EPDiO54734.
MaxQBiO54734.
PaxDbiO54734.
PeptideAtlasiO54734.
PRIDEiO54734.
TopDownProteomicsiO54734.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030538; ENSMUSP00000030538; ENSMUSG00000028757.
GeneIDi13200.
KEGGimmu:13200.
UCSCiuc008vkt.1. mouse.

Organism-specific databases

CTDi1650.
MGIiMGI:1194508. Ddost.

Phylogenomic databases

eggNOGiKOG2754. Eukaryota.
ENOG410XSF3. LUCA.
GeneTreeiENSGT00390000017294.
HOGENOMiHOG000173744.
HOVERGENiHBG053378.
InParanoidiO54734.
KOiK12670.
OMAiAFSMMIG.
OrthoDBiEOG091G07Z5.
TreeFamiTF314821.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

ChiTaRSiDdost. mouse.
PROiO54734.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028757.
GenevisibleiO54734. MM.

Family and domain databases

InterProiIPR005013. DDOST_48_kDa_subunit.
[Graphical view]
PANTHERiPTHR10830. PTHR10830. 1 hit.
PfamiPF03345. DDOST_48kD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOST48_MOUSE
AccessioniPrimary (citable) accession number: O54734
Secondary accession number(s): Q8C4P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.