O54734 (OST48_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Gene names

Name:

Ddost

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	By similarity
	Chain	29 – 441	413	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000021958
Regions
	Topological domain	29 – 412	384	Lumenal Potential
	Transmembrane	413 – 432	20	Helical; Potential
	Topological domain	433 – 441	9	Cytoplasmic Potential
Experimental info
	Sequence conflict	321	1	I → V in BAA23671. Ref.1
	Sequence conflict	321	1	I → V in BAB82434. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O54734 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: C1B9A0B94D2A1CAC	FASTA	441	49,028
	10 20 30 40 50 60 MKMDPRLAVR AWPLCGLLLA VLGCVCASGP RTLVLLDNLN VRDTHSLFFR SLKDRGFELT 70 80 90 100 110 120 FKTADDPSLS LIKYGEFLYD NLIIFSPSVE DFGGNINVET ISAFIDGGGS VLVAASSDIG 130 140 150 160 170 180 DPLRELGSEC GIEFDEEKTA VIDHHNYDVS DLGQHTLIVA DTENLLKAPT IVGKSSLNPI 190 200 210 220 230 240 LFRGVGMVAD PDNPLVLDIL TGSSTSYSFF PDKPITQYPH AVGRNTLLIA GLQARNNARV 250 260 270 280 290 300 IFSGSLDFFS DAFFNSAVQK ATPGAQRYSQ TGNYELAVAL SRWVFKEEGV LRVGPVSHHR 310 320 330 340 350 360 VGEMAPPNAY TVTDLVEYSI IIEQLSNGKW VPFDGDDIQL EFVRIDPFVR TFLKRKGGKY 370 380 390 400 410 420 SVQFKLPDVY GVFQFKVDYN RLGYTHLYSS TQVSVRPLQH TQYERFIPSA YPYYASAFSM 430 440 MAGLFIFSIV FLHMKEKEKS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome organization of human 48-kDa oligosaccharyltransferase (DDOST)." Yamagata T., Tsuru T., Momoi M.Y., Suwa K., Nozaki Y., Mukasa T., Ohashi H., Fukushima Y., Momoi T. Genomics 45:535-540(1997) [PubMed: 9367678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse 48-kDa oligosaccharyltransferase (DDOST)." Tsuru T. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Head.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[6]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 32-42; 175-183; 225-235 AND 366-376, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D89063 mRNA. Translation: BAA23671.1. AB012717 Genomic DNA. Translation: BAB82434.1. AK081542 mRNA. Translation: BAC38250.1. AL807249 Genomic DNA. Translation: CAM18871.1. BC068132 mRNA. Translation: AAH68132.1.
IPI	IPI00117705.
RefSeq	NP_031864.2. NM_007838.2.
UniGene	Mm.7236.
3D structure databases
ProteinModelPortal	O54734.
ModBase	Search...
Protein-protein interaction databases
IntAct	O54734. 1 interaction.
STRING	O54734.
PTM databases
PhosphoSite	O54734.
Proteomic databases
PRIDE	O54734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000030538; ENSMUSP00000030538; ENSMUSG00000028757.
GeneID	13200.
KEGG	mmu:13200.
Organism-specific databases
CTD	1650.
MGI	MGI:1194508. Ddost.
Phylogenomic databases
eggNOG	roNOG09724.
HOGENOM	HBG317312.
HOVERGEN	HBG053378.
InParanoid	O54734.
OrthoDB	EOG45HRXC.
PhylomeDB	O54734.
Gene expression databases
ArrayExpress	O54734.
Bgee	O54734.
Genevestigator	O54734.
GermOnline	ENSMUSG00000028757. Mus musculus.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
KO	K12670.
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

OST48_MOUSE

Accession

Primary (citable) accession number: O54734
Secondary accession number(s): Q8C4P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program