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O54732 (MMP15_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Matrix metalloproteinase-15

Short name=MMP-15
EC=3.4.24.-
Alternative name(s):
Membrane-type matrix metalloproteinase 2
Short name=MT-MMP 2
Short name=MTMMP2
Membrane-type-2 matrix metalloproteinase
Short name=MT2-MMP
Short name=MT2MMP
Gene names
Name:Mmp15
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Calcium By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein; Extracellular side Potential.

Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Post-translational modification

The precursor is cleaved by a furin endopeptidase By similarity.

Sequence similarities

Belongs to the peptidase M10A family.

Contains 4 hemopexin repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3636 Potential
Propeptide37 – 12791 By similarity
PRO_0000028810
Chain128 – 657530Matrix metalloproteinase-15
PRO_0000028811

Regions

Topological domain128 – 614487Extracellular Potential
Transmembrane615 – 63521Helical; Potential
Topological domain636 – 65722Cytoplasmic Potential
Repeat363 – 41149Hemopexin 1
Repeat412 – 45746Hemopexin 2
Repeat459 – 50749Hemopexin 3
Repeat508 – 55548Hemopexin 4
Motif105 – 1128Cysteine switch By similarity
Compositional bias122 – 1276Poly-Arg

Sites

Active site2561 By similarity
Metal binding1071Zinc; in inhibited form By similarity
Metal binding2551Zinc; catalytic By similarity
Metal binding2591Zinc; catalytic By similarity
Metal binding2651Zinc; catalytic By similarity

Amino acid modifications

Glycosylation1461N-linked (GlcNAc...) Potential
Glycosylation4141N-linked (GlcNAc...) Potential
Disulfide bond366 ↔ 555 By similarity

Sequences

Sequence LengthMass (Da)Tools
O54732 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 26803400A6836EF2

FASTA65774,666
        10         20         30         40         50         60 
MGSDRSALGR PGCTGSCLSS RASLLPLLLV LLDCLGHGTA SKDAEVYAAE NWLRLYGYLP 

        70         80         90        100        110        120 
QPSRHMSTMR SAQILASALA EMQSFYGIPV TGVLDEETKT WMKRPRCGVP DQFGVHVKAN 

       130        140        150        160        170        180 
LRRRRKRYTL TGKAWNNYHL TFSIQNYTEK LGWYNSMEAV RRAFQVWEQV TPLVFQEVSY 

       190        200        210        220        230        240 
DDIRLRRRAE ADIMVLFASG FHGDSSPFDG VGGFLAHAYF PGPGLGGDTH FDADEPWTFS 

       250        260        270        280        290        300 
STDLHGISLF LVAVHELGHA LGLEHSSNPS AIMAPFYQWM DTDNFQLPED DLRGIQQLYG 

       310        320        330        340        350        360 
SPDGKPQPTR PLPTVRPRRP GRPDHQPPRP PQPPHPGGKP ERPPKPGPPP QPRATERPDQ 

       370        380        390        400        410        420 
YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY PMPIGHFWRG LPGNISAAYE 

       430        440        450        460        470        480 
RQDGHFVFFK GNRYWLFREA NLEPGYPQPL SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD 

       490        500        510        520        530        540 
RYWRFNEETQ HGDPGYPKPI SVWQGIPTSP KGAFLSNDAA YTYFYKGTKY WKFNNERLRM 

       550        560        570        580        590        600 
EPGHPKSILR DFMGCQEHVE PRSRWPDVAR PPFNPNGGAE PEADGDSKEE NAGDKDEGSR 

       610        620        630        640        650 
VVVQMEEVVR TVNVVMVLVP LLLLLCILGL AFALVQMQRK GAPRMLLYCK RSLQEWV 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of a mouse MT2-MMP gene from a lung cDNA library and identification of its product."
Tanaka M., Sato H., Takino T., Iwata K., Inoue M., Seiki M.
FEBS Lett. 402:219-222(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Lung.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary gland and Olfactory epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86332 mRNA. Translation: BAA23667.1.
BC047278 mRNA. Translation: AAH47278.1.
BC057952 mRNA. Translation: AAH57952.1.
CCDSCCDS22561.1.
RefSeqNP_032635.1. NM_008609.3.
UniGeneMm.217116.

3D structure databases

ProteinModelPortalO54732.
SMRO54732. Positions 79-555.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000034243.

Protein family/group databases

MEROPSM10.015.

PTM databases

PhosphoSiteO54732.

Proteomic databases

PRIDEO54732.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034243; ENSMUSP00000034243; ENSMUSG00000031790.
GeneID17388.
KEGGmmu:17388.
UCSCuc009myh.1. mouse.

Organism-specific databases

CTD4324.
MGIMGI:109320. Mmp15.

Phylogenomic databases

eggNOGNOG295915.
GeneTreeENSGT00750000117332.
HOGENOMHOG000217928.
HOVERGENHBG052484.
InParanoidO54732.
KOK07995.
OMAVQMQRKG.
OrthoDBEOG7XPZ57.
PhylomeDBO54732.
TreeFamTF352396.

Gene expression databases

ArrayExpressO54732.
BgeeO54732.
CleanExMM_MMP15.
GenevestigatorO54732.

Family and domain databases

Gene3D2.110.10.10. 1 hit.
3.40.390.10. 1 hit.
InterProIPR000585. Hemopexin-like_dom.
IPR018487. Hemopexin-like_repeat.
IPR018486. Hemopexin_CS.
IPR024079. MetalloPept_cat_dom.
IPR028729. MMP15.
IPR001818. Pept_M10_metallopeptidase.
IPR021190. Pept_M10A.
IPR021805. Pept_M10A_metallopeptidase_C.
IPR016293. Pept_M10A_stromelysin-type.
IPR021158. Pept_M10A_Zn_BS.
IPR006026. Peptidase_Metallo.
IPR002477. Peptidoglycan-bd-like.
[Graphical view]
PANTHERPTHR10201:SF25. PTHR10201:SF25. 1 hit.
PfamPF11857. DUF3377. 1 hit.
PF00045. Hemopexin. 4 hits.
PF00413. Peptidase_M10. 1 hit.
PF01471. PG_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTSPR00138. MATRIXIN.
SMARTSM00120. HX. 4 hits.
SM00235. ZnMc. 1 hit.
[Graphical view]
SUPFAMSSF47090. SSF47090. 1 hit.
SSF50923. SSF50923. 1 hit.
PROSITEPS00546. CYSTEINE_SWITCH. 1 hit.
PS00024. HEMOPEXIN. 1 hit.
PS51642. HEMOPEXIN_2. 4 hits.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292004.
PROO54732.
SOURCESearch...

Entry information

Entry nameMMP15_MOUSE
AccessionPrimary (citable) accession number: O54732
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot