Matrix metalloproteinase-15 precursor

Contribute

Send feedback

Read comments (?) or add your own

O54732 (MMP15_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Matrix metalloproteinase-15
Short name=MMP-15
EC=3.4.24.-

Alternative name(s):
Membrane-type matrix metalloproteinase 2
Short name=MT-MMP 2
Short name=MTMMP2
Membrane-type-2 matrix metalloproteinase
Short name=MT2-MMP
Short name=MT2MMP

Gene names

Name:

Mmp15

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Cofactor	Binds 1 zinc ion per subunit By similarity. Calcium By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein; Extracellular side Potential.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW response to estradiol stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular matrix Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro metalloendopeptidase activity Inferred from sequence or structural similarity PubMed 9048885. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 36

Potential

Propeptide

37 – 127

By similarity

PRO_0000028810

Chain

128 – 657

530

Matrix metalloproteinase-15

PRO_0000028811

Regions

Topological domain

128 – 614

487

Extracellular Potential

Transmembrane

615 – 635

Helical; Potential

Topological domain

636 – 657

Cytoplasmic Potential

Domain

370 – 413

Hemopexin-like 1

Domain

415 – 459

Hemopexin-like 2

Domain

462 – 508

Hemopexin-like 3

Domain

510 – 555

Hemopexin-like 4

Motif

105 – 112

Cysteine switch By similarity

Compositional bias

122 – 127

Poly-Arg

Sites

Active site

256

By similarity

Metal binding

107

Zinc; in inhibited form By similarity

Metal binding

255

Zinc; catalytic By similarity

Metal binding

259

Zinc; catalytic By similarity

Metal binding

265

Zinc; catalytic By similarity

Amino acid modifications

Glycosylation

146

N-linked (GlcNAc...) Potential

Glycosylation

414

N-linked (GlcNAc...) Potential

Disulfide bond

366 ↔ 555

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O54732 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 26803400A6836EF2
Show »

FASTA

657

74,666

        10         20         30         40         50         60 
MGSDRSALGR PGCTGSCLSS RASLLPLLLV LLDCLGHGTA SKDAEVYAAE NWLRLYGYLP 

        70         80         90        100        110        120 
QPSRHMSTMR SAQILASALA EMQSFYGIPV TGVLDEETKT WMKRPRCGVP DQFGVHVKAN 

       130        140        150        160        170        180 
LRRRRKRYTL TGKAWNNYHL TFSIQNYTEK LGWYNSMEAV RRAFQVWEQV TPLVFQEVSY 

       190        200        210        220        230        240 
DDIRLRRRAE ADIMVLFASG FHGDSSPFDG VGGFLAHAYF PGPGLGGDTH FDADEPWTFS 

       250        260        270        280        290        300 
STDLHGISLF LVAVHELGHA LGLEHSSNPS AIMAPFYQWM DTDNFQLPED DLRGIQQLYG 

       310        320        330        340        350        360 
SPDGKPQPTR PLPTVRPRRP GRPDHQPPRP PQPPHPGGKP ERPPKPGPPP QPRATERPDQ 

       370        380        390        400        410        420 
YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY PMPIGHFWRG LPGNISAAYE 

       430        440        450        460        470        480 
RQDGHFVFFK GNRYWLFREA NLEPGYPQPL SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD 

       490        500        510        520        530        540 
RYWRFNEETQ HGDPGYPKPI SVWQGIPTSP KGAFLSNDAA YTYFYKGTKY WKFNNERLRM 

       550        560        570        580        590        600 
EPGHPKSILR DFMGCQEHVE PRSRWPDVAR PPFNPNGGAE PEADGDSKEE NAGDKDEGSR 

       610        620        630        640        650 
VVVQMEEVVR TVNVVMVLVP LLLLLCILGL AFALVQMQRK GAPRMLLYCK RSLQEWV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a mouse MT2-MMP gene from a lung cDNA library and identification of its product." Tanaka M., Sato H., Takino T., Iwata K., Inoue M., Seiki M. FEBS Lett. 402:219-222(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NMRI. Tissue: Mammary gland and Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D86332 mRNA. Translation: BAA23667.1. BC047278 mRNA. Translation: AAH47278.1. BC057952 mRNA. Translation: AAH57952.1.
IPI	IPI00117704.
RefSeq	NP_032635.1. NM_008609.3.
UniGene	Mm.217116.
3D structure databases
ProteinModelPortal	O54732.
SMR	O54732. Positions 79-555.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000034243.
Protein family/group databases
MEROPS	M10.015.
PTM databases
PhosphoSite	O54732.
Proteomic databases
PRIDE	O54732.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034243; ENSMUSP00000034243; ENSMUSG00000031790.
GeneID	17388.
KEGG	mmu:17388.
UCSC	uc009myh.1. mouse.
Organism-specific databases
CTD	4324.
MGI	MGI:109320. Mmp15.
Phylogenomic databases
eggNOG	NOG295915.
GeneTree	ENSGT00700000104046.
HOGENOM	HOG000217928.
HOVERGEN	HBG052484.
InParanoid	O54732.
KO	K07995.
OMA	VQMQRKG.
OrthoDB	EOG4W6NVP.
Gene expression databases
ArrayExpress	O54732.
Bgee	O54732.
CleanEx	MM_MMP15.
Genevestigator	O54732.
GermOnline	ENSMUSG00000031790. Mus musculus.
Family and domain databases
Gene3D	2.110.10.10. 1 hit. 3.40.390.10. 1 hit.
InterPro	IPR000585. Hemopexin-like_dom. IPR018487. Hemopexin-like_repeat. IPR018486. Hemopexin_CS. IPR024079. MetalloPept_cat_dom. IPR001818. Pept_M10_metallopeptidase. IPR021190. Pept_M10A. IPR021805. Pept_M10A_metallopeptidase_C. IPR016293. Pept_M10A_Metazoans. IPR021158. Pept_M10A_Zn_BS. IPR006026. Peptidase_Metallo. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Pfam	PF11857. DUF3377. 1 hit. PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
SUPFAM	SSF50923. Hemopexin. 1 hit. SSF47090. PGBD_like. 1 hit.
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	292004.
SOURCE	Search...

Entry information

Entry name

MMP15_MOUSE

Accession

Primary (citable) accession number: O54732

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents