Reviewed, UniProtKB/Swiss-Prot O54732 (MMP15_MOUSE)

Names and origin

Protein names

Recommended name:
    Matrix metalloproteinase-15
      Short name=MMP-15
    EC=3.4.24.-
Alternative name(s):
    Membrane-type matrix metalloproteinase 2
      Short name=MT-MMP 2
      Short name=MTMMP2
    Membrane-type-2 matrix metalloproteinase
      Short name=MT2-MMP
      Short name=MT2MMP

Gene names

Name:

Mmp15

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
Cofactor	Binds 1 zinc ion per subunit By similarity. Calcium By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein; Extracellular side Potential.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Post-translational modification	The precursor is cleaved by a furin endopeptidase By similarity.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW proteinaceous extracellular matrix Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Propeptide	37 – 127	91	By similarity		PRO_0000028810
	Chain	128 – 657	530	Matrix metalloproteinase-15		PRO_0000028811
Regions
	Topological domain	128 – 614	487	Extracellular Potential
	Transmembrane	615 – 635	21	Potential
	Topological domain	636 – 657	22	Cytoplasmic Potential
	Domain	370 – 413	44	Hemopexin-like 1
	Domain	415 – 459	45	Hemopexin-like 2
	Domain	462 – 508	47	Hemopexin-like 3
	Domain	510 – 555	46	Hemopexin-like 4
	Motif	105 – 112	8	Cysteine switch By similarity
	Compositional bias	122 – 127	6	Poly-Arg
Sites
	Active site	256	1	By similarity
	Metal binding	107	1	Zinc; in inhibited form By similarity
	Metal binding	255	1	Zinc; catalytic By similarity
	Metal binding	259	1	Zinc; catalytic By similarity
	Metal binding	265	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	146	1	N-linked (GlcNAc...) Potential
	Glycosylation	414	1	N-linked (GlcNAc...) Potential
	Disulfide bond	366 ↔ 555		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54732-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 26803400A6836EF2	FASTA	657	74,666
	10 20 30 40 50 60 MGSDRSALGR PGCTGSCLSS RASLLPLLLV LLDCLGHGTA SKDAEVYAAE NWLRLYGYLP 70 80 90 100 110 120 QPSRHMSTMR SAQILASALA EMQSFYGIPV TGVLDEETKT WMKRPRCGVP DQFGVHVKAN 130 140 150 160 170 180 LRRRRKRYTL TGKAWNNYHL TFSIQNYTEK LGWYNSMEAV RRAFQVWEQV TPLVFQEVSY 190 200 210 220 230 240 DDIRLRRRAE ADIMVLFASG FHGDSSPFDG VGGFLAHAYF PGPGLGGDTH FDADEPWTFS 250 260 270 280 290 300 STDLHGISLF LVAVHELGHA LGLEHSSNPS AIMAPFYQWM DTDNFQLPED DLRGIQQLYG 310 320 330 340 350 360 SPDGKPQPTR PLPTVRPRRP GRPDHQPPRP PQPPHPGGKP ERPPKPGPPP QPRATERPDQ 370 380 390 400 410 420 YGPNICDGNF DTVAVLRGEM FVFKGRWFWR VRHNRVLDNY PMPIGHFWRG LPGNISAAYE 430 440 450 460 470 480 RQDGHFVFFK GNRYWLFREA NLEPGYPQPL SSYGTDIPYD RIDTAIWWEP TGHTFFFQAD 490 500 510 520 530 540 RYWRFNEETQ HGDPGYPKPI SVWQGIPTSP KGAFLSNDAA YTYFYKGTKY WKFNNERLRM 550 560 570 580 590 600 EPGHPKSILR DFMGCQEHVE PRSRWPDVAR PPFNPNGGAE PEADGDSKEE NAGDKDEGSR 610 620 630 640 650 VVVQMEEVVR TVNVVMVLVP LLLLLCILGL AFALVQMQRK GAPRMLLYCK RSLQEWV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a mouse MT2-MMP gene from a lung cDNA library and identification of its product." Tanaka M., Sato H., Takino T., Iwata K., Inoue M., Seiki M. FEBS Lett. 402:219-222(1997) [PubMed: 9037199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NMRI. Tissue: Mammary gland and Olfactory epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86332 mRNA. Translation: BAA23667.1. BC047278 mRNA. Translation: AAH47278.1. BC057952 mRNA. Translation: AAH57952.1.
IPI	IPI00117704.
RefSeq	NP_032635.1.
UniGene	Mm.217116
3D structure databases
HSSP	HSSP built from PDB template 1CXV based on UniProtKB P33435.
ModBase	Search...
Protein family/group databases
MEROPS	M10.015.
PTM databases
PhosphoSite	O54732.
Genome annotation databases
Ensembl	ENSMUSG00000031790. Mus musculus. [Contig view]
GeneID	17388.
KEGG	mmu:17388.
Organism-specific databases
MGI	MGI:109320. Mmp15.
Phylogenomic databases
HOGENOM	O54732.
HOVERGEN	O54732.
OMA	O54732. ADEPWTF.
Gene expression databases
ArrayExpress	O54732.
Bgee	O54732.
CleanEx	MM_MMP15.
GermOnline	ENSMUSG00000031790. Mus musculus.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR001818. Pept_M10A_M12B. IPR016293. Pept_M10A_matrix. IPR006025. Pept_M_Zn_BS. IPR006026. Peptidase_M. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:2.110.10.10. Hemopexin. 1 hit.
Pfam	PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	292004.
SOURCE	Search...

Entry information

Entry name

MMP15_MOUSE

Accession

Primary (citable) accession number: O54732

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)