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O54728

- PLB1_RAT

UniProt

O54728 - PLB1_RAT

Protein

Phospholipase B1, membrane-associated

Gene

Plb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids.3 Publications

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Inhibited by diisopropyl fluorophosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei404 – 4041NucleophileBy similarity
    Binding sitei478 – 4781Substrate; via amide nitrogenBy similarity
    Binding sitei517 – 5171SubstrateBy similarity
    Active sitei1351 – 13511By similarity
    Active sitei1354 – 13541By similarity

    GO - Molecular functioni

    1. lysophospholipase activity Source: RGD
    2. phospholipase A2 activity Source: RGD
    3. retinyl-palmitate esterase activity Source: RGD

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. phospholipid metabolic process Source: RGD
    3. retinol metabolic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase B1, membrane-associated
    Short name:
    Phospholipase B
    Alternative name(s):
    Phospholipase B/lipase
    Short name:
    PLB/LIP
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:Plb1
    Synonyms:Phlpb
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi621565. Plb1.

    Subcellular locationi

    Apical cell membrane 2 Publications; Single-pass type I membrane protein 2 Publications
    Note: Present in the intestinal brush border membranes.

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. brush border membrane Source: RGD
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi404 – 4041S → A or C: Loss of activity. 1 Publication
    Mutagenesisi414 – 4141S → A: Decrease in activity. 1 Publication
    Mutagenesisi429 – 4291S → A: Decrease in activity. 1 Publication
    Mutagenesisi459 – 4591S → A: Decrease in activity. 1 Publication
    Mutagenesisi518 – 5181D → A or V: Loss of activity. 1 Publication
    Mutagenesisi518 – 5181D → E or N: Decrease in activity. 1 Publication
    Mutagenesisi659 – 6591H → A: Loss of activity. 1 Publication
    Mutagenesisi665 – 6651H → A: Slight effect on activity. 1 Publication
    Mutagenesisi686 – 6861H → A: No effect on activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 14501427Phospholipase B1, membrane-associatedPRO_0000324386Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi1059 – 10591N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes proteolytic cleavage in the ileum.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO54728.
    PRIDEiO54728.

    PTM databases

    PhosphoSiteiO54728.

    Expressioni

    Tissue specificityi

    Expressed in the ileum mucosa, Paneth cells spermatocytes, spermatids and sperm (at protein level). Expressed in the ileum, jejunum, esophagus and testis.2 Publications

    Gene expression databases

    GenevestigatoriO54728.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000057888.

    Structurei

    3D structure databases

    ProteinModelPortaliO54728.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 14221399ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1444 – 14507CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1423 – 144321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati41 – 3513111Add
    BLAST
    Repeati366 – 7113462Add
    BLAST
    Repeati712 – 10583473Add
    BLAST
    Repeati1068 – 14073404Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni41 – 140713674 X 308-326 AA approximate repeatsAdd
    BLAST
    Regioni1408 – 145043Necessary for membrane localizationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili590 – 61728Sequence AnalysisAdd
    BLAST

    Domaini

    Repeat 2 contains the catalytic domain.

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG311176.
    HOGENOMiHOG000115574.
    HOVERGENiHBG108263.
    InParanoidiO54728.
    KOiK14621.
    PhylomeDBiO54728.

    Family and domain databases

    Gene3Di3.40.50.1110. 5 hits.
    InterProiIPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]
    PfamiPF00657. Lipase_GDSL. 3 hits.
    [Graphical view]
    PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O54728-1 [UniParc]FASTAAdd to Basket

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    MESWPGVSLV GLLLLLLLGQ GPSQIHGSSG ENTSQPQQVF RTLKNFSFPC     50
    KPKKLELSVL SKSVHSLRPS DIKLVAAIGN LETPPAPGSG VVNMEKPQSL 100
    ESELQNVCIG IMTALSDIIR HFNPSVLMPT CSPGKGTAGH TTIAEDLWIQ 150
    AKELVRHLKD NPELDFEKDW KLITVLFSNT SQCHLCSSDQ QKRHLMKHME 200
    MLSGVLDYLH REVPRAFVNL VDLSEVLTMA QQHQETGFSP APEICKCSEE 250
    ITKLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQSFF SEVELPLERP 300
    SPQDSTTLAL RIWNSMMEPV GRKDGTLNEA ERKTMKCPSQ ESPYLFTYRN 350
    SNYQARQLKP IGKFQMKEGT KFTCPDKDPS DSIPTTVHRL RPADIKVIGA 400
    MGDSLTAGNG AGSSPGNVLD VLTQYRGLSW SVGGDETIET VTTLANILRE 450
    FNPSLKGFSV GTGKENTPRA SFNQAVAGAK SDGLAAQAKK LVSLMKDDKT 500
    INFQEDWKII TVFIGGNDLC GSCNNLARFS PQTFTDNIKT ALDILHAEVP 550
    RAFVNMVSVI EITPLRELFN EPKVSCPRMI LRSLCPCVLN LGENSAELAQ 600
    LVERNRQYQE ETGKLIESGR YDTRDDFTVV LQPMFENVVM PRTLEGLPDS 650
    SFFAPDCFHF NVKTHARSAI ALWKNMLEPV GRKTRHQNFE IKVPIMCPNQ 700
    TSPFLSTTKN SNLGHGTSMS CEEKAPSASP PTSVHTLRPA DIQVVAALGD 750
    SVTAGNGISS QEGDLADVTT QYRGLSYSAG GDKFLENVTT LPNILREFNG 800
    NLTGYSVGTG DVNSASAFLN QAVPGAKAEN LASQVQTLIQ KMKNDTRVNF 850
    HQDWKVITVM IGASDLCDFC KDSNRYSAAN FSDHLRNALD ILHKEVPRAL 900
    VNLVDFMNPS IIRQVFLKNP DKCPVNQTSV LCNCVLTPGE DSHELARLEA 950
    FTKSYQSSML QLVESGRYDT REDFSVVLQP FLFNIRLPIL ENGNPDTSFF 1000
    APDCILLSQK FHTQLARALW ANMLEPLGKK MDTLDPKELI ALACPTKDKP 1050
    FLRTFRNSNY TYPIKPAIEN WGSDFLCTEQ SPSSKVPTSV HELRPSDIKV 1100
    VAAMGDFLTT ATGARPSESS SLDTPWRGLS WSIGGDGTLE THTTLPNILK 1150
    KFNPSILGFS TGTLENTAGL NVAEEGARAQ DMPAQAQALV KKMKSTPTIN 1200
    IQEDWKLITL LIGNNDLCLY CEDPENYSTR EYVKYIQHAL DIFYEELPRV 1250
    FINVVEVMEL SGLLHDQGGK CAMPLAVQKN CSCLKRSQNL MAMQELKKVN 1300
    GNLQSALSEL SYWHRYMQRE DFAVTVQPFF RNTFVPLDER GGLDLTFFSE 1350
    DCFHFSVRGH AEMAIALWNN MLEPVGKKTT SNNFTYNRTK LKCPSPENPF 1400
    LYTVRNSQIL LDKAKENSNT LYWAVPVAAV GGLVVGILGM MLWRTVRLVQ 1450
    Length:1,450
    Mass (Da):161,089
    Last modified:June 1, 1998 - v1
    Checksum:i4555898C8FD91F45
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63648 mRNA. Translation: BAA23813.1.
    RefSeqiNP_620253.1. NM_138898.1.
    UniGeneiRn.91079.

    Genome annotation databases

    GeneIDi192259.
    KEGGirno:192259.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63648 mRNA. Translation: BAA23813.1 .
    RefSeqi NP_620253.1. NM_138898.1.
    UniGenei Rn.91079.

    3D structure databases

    ProteinModelPortali O54728.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000057888.

    PTM databases

    PhosphoSitei O54728.

    Proteomic databases

    PaxDbi O54728.
    PRIDEi O54728.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 192259.
    KEGGi rno:192259.

    Organism-specific databases

    CTDi 151056.
    RGDi 621565. Plb1.

    Phylogenomic databases

    eggNOGi NOG311176.
    HOGENOMi HOG000115574.
    HOVERGENi HBG108263.
    InParanoidi O54728.
    KOi K14621.
    PhylomeDBi O54728.

    Miscellaneous databases

    NextBioi 622930.
    PROi O54728.

    Gene expression databases

    Genevestigatori O54728.

    Family and domain databases

    Gene3Di 3.40.50.1110. 5 hits.
    InterProi IPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    Pfami PF00657. Lipase_GDSL. 3 hits.
    [Graphical view ]
    PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of functional domains of rat intestinal phospholipase B/lipase. Its cDNA cloning, expression, and tissue distribution."
      Takemori H., Zolotaryov F.N., Ting L., Urbain T., Komatsubara T., Hatano O., Okamoto M., Tojo H.
      J. Biol. Chem. 273:2222-2231(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Ileal mucosa.
    2. "Purification and characterization of a catalytic domain of rat intestinal phospholipase B/lipase associated with brush border membranes."
      Tojo H., Ichida T., Okamoto M.
      J. Biol. Chem. 273:2214-2221(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 368-380 AND 529-559, FUNCTION, SUBCELLULAR LOCATION.
    3. "Further characterization of a novel phospholipase B (phospholipase A2-lysophospholipase) from intestinal brush-border membranes."
      Pind S., Kuksis A.
      Biochem. Cell Biol. 69:346-357(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. "Identification of essential residues for catalysis of rat intestinal phospholipase B/lipase."
      Lu T., Ito M., Tchoua U., Takemori H., Okamoto M., Tojo H.
      Biochemistry 40:7133-7139(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF SER-404; SER-414; SER-429; SER-459; ASP-518; HIS-659; HIS-665 AND HIS-686.

    Entry informationi

    Entry nameiPLB1_RAT
    AccessioniPrimary (citable) accession number: O54728
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3