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Protein

Phospholipase B1, membrane-associated

Gene

Plb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids.3 Publications

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei404NucleophileBy similarity1
Binding sitei478Substrate; via amide nitrogenBy similarity1
Binding sitei517SubstrateBy similarity1
Active sitei1351By similarity1
Active sitei1354By similarity1

GO - Molecular functioni

  • lysophospholipase activity Source: RGD
  • phospholipase A2 activity Source: RGD
  • retinyl-palmitate esterase activity Source: RGD

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • phospholipid metabolic process Source: RGD
  • retinol metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Chemistry databases

SwissLipidsiSLP:000000624.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Plb1
Synonyms:Phlpb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621565. Plb1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 1422ExtracellularSequence analysisAdd BLAST1399
Transmembranei1423 – 1443HelicalSequence analysisAdd BLAST21
Topological domaini1444 – 1450CytoplasmicSequence analysis7

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB-SubCell
  • brush border membrane Source: RGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi404S → A or C: Loss of activity. 1 Publication1
Mutagenesisi414S → A: Decrease in activity. 1 Publication1
Mutagenesisi429S → A: Decrease in activity. 1 Publication1
Mutagenesisi459S → A: Decrease in activity. 1 Publication1
Mutagenesisi518D → A or V: Loss of activity. 1 Publication1
Mutagenesisi518D → E or N: Decrease in activity. 1 Publication1
Mutagenesisi659H → A: Loss of activity. 1 Publication1
Mutagenesisi665H → A: Slight effect on activity. 1 Publication1
Mutagenesisi686H → A: No effect on activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000032438624 – 1450Phospholipase B1, membrane-associatedAdd BLAST1427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi801N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1059N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54728.
PRIDEiO54728.

PTM databases

iPTMnetiO54728.
PhosphoSitePlusiO54728.

Expressioni

Tissue specificityi

Expressed in the ileum mucosa, Paneth cells spermatocytes, spermatids and sperm (at protein level). Expressed in the ileum, jejunum, esophagus and testis.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057888.

Structurei

3D structure databases

ProteinModelPortaliO54728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati41 – 3511Add BLAST311
Repeati366 – 7112Add BLAST346
Repeati712 – 10583Add BLAST347
Repeati1068 – 14074Add BLAST340

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni41 – 14074 X 308-326 AA approximate repeatsAdd BLAST1367
Regioni1408 – 1450Necessary for membrane localizationAdd BLAST43

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili590 – 617Sequence analysisAdd BLAST28

Domaini

Repeat 2 contains the catalytic domain.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3670. Eukaryota.
ENOG410ZM9P. LUCA.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiO54728.
KOiK14621.
PhylomeDBiO54728.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 7 hits.
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54728-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESWPGVSLV GLLLLLLLGQ GPSQIHGSSG ENTSQPQQVF RTLKNFSFPC
60 70 80 90 100
KPKKLELSVL SKSVHSLRPS DIKLVAAIGN LETPPAPGSG VVNMEKPQSL
110 120 130 140 150
ESELQNVCIG IMTALSDIIR HFNPSVLMPT CSPGKGTAGH TTIAEDLWIQ
160 170 180 190 200
AKELVRHLKD NPELDFEKDW KLITVLFSNT SQCHLCSSDQ QKRHLMKHME
210 220 230 240 250
MLSGVLDYLH REVPRAFVNL VDLSEVLTMA QQHQETGFSP APEICKCSEE
260 270 280 290 300
ITKLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQSFF SEVELPLERP
310 320 330 340 350
SPQDSTTLAL RIWNSMMEPV GRKDGTLNEA ERKTMKCPSQ ESPYLFTYRN
360 370 380 390 400
SNYQARQLKP IGKFQMKEGT KFTCPDKDPS DSIPTTVHRL RPADIKVIGA
410 420 430 440 450
MGDSLTAGNG AGSSPGNVLD VLTQYRGLSW SVGGDETIET VTTLANILRE
460 470 480 490 500
FNPSLKGFSV GTGKENTPRA SFNQAVAGAK SDGLAAQAKK LVSLMKDDKT
510 520 530 540 550
INFQEDWKII TVFIGGNDLC GSCNNLARFS PQTFTDNIKT ALDILHAEVP
560 570 580 590 600
RAFVNMVSVI EITPLRELFN EPKVSCPRMI LRSLCPCVLN LGENSAELAQ
610 620 630 640 650
LVERNRQYQE ETGKLIESGR YDTRDDFTVV LQPMFENVVM PRTLEGLPDS
660 670 680 690 700
SFFAPDCFHF NVKTHARSAI ALWKNMLEPV GRKTRHQNFE IKVPIMCPNQ
710 720 730 740 750
TSPFLSTTKN SNLGHGTSMS CEEKAPSASP PTSVHTLRPA DIQVVAALGD
760 770 780 790 800
SVTAGNGISS QEGDLADVTT QYRGLSYSAG GDKFLENVTT LPNILREFNG
810 820 830 840 850
NLTGYSVGTG DVNSASAFLN QAVPGAKAEN LASQVQTLIQ KMKNDTRVNF
860 870 880 890 900
HQDWKVITVM IGASDLCDFC KDSNRYSAAN FSDHLRNALD ILHKEVPRAL
910 920 930 940 950
VNLVDFMNPS IIRQVFLKNP DKCPVNQTSV LCNCVLTPGE DSHELARLEA
960 970 980 990 1000
FTKSYQSSML QLVESGRYDT REDFSVVLQP FLFNIRLPIL ENGNPDTSFF
1010 1020 1030 1040 1050
APDCILLSQK FHTQLARALW ANMLEPLGKK MDTLDPKELI ALACPTKDKP
1060 1070 1080 1090 1100
FLRTFRNSNY TYPIKPAIEN WGSDFLCTEQ SPSSKVPTSV HELRPSDIKV
1110 1120 1130 1140 1150
VAAMGDFLTT ATGARPSESS SLDTPWRGLS WSIGGDGTLE THTTLPNILK
1160 1170 1180 1190 1200
KFNPSILGFS TGTLENTAGL NVAEEGARAQ DMPAQAQALV KKMKSTPTIN
1210 1220 1230 1240 1250
IQEDWKLITL LIGNNDLCLY CEDPENYSTR EYVKYIQHAL DIFYEELPRV
1260 1270 1280 1290 1300
FINVVEVMEL SGLLHDQGGK CAMPLAVQKN CSCLKRSQNL MAMQELKKVN
1310 1320 1330 1340 1350
GNLQSALSEL SYWHRYMQRE DFAVTVQPFF RNTFVPLDER GGLDLTFFSE
1360 1370 1380 1390 1400
DCFHFSVRGH AEMAIALWNN MLEPVGKKTT SNNFTYNRTK LKCPSPENPF
1410 1420 1430 1440 1450
LYTVRNSQIL LDKAKENSNT LYWAVPVAAV GGLVVGILGM MLWRTVRLVQ
Length:1,450
Mass (Da):161,089
Last modified:June 1, 1998 - v1
Checksum:i4555898C8FD91F45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63648 mRNA. Translation: BAA23813.1.
RefSeqiNP_620253.1. NM_138898.1.
UniGeneiRn.91079.

Genome annotation databases

GeneIDi192259.
KEGGirno:192259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63648 mRNA. Translation: BAA23813.1.
RefSeqiNP_620253.1. NM_138898.1.
UniGeneiRn.91079.

3D structure databases

ProteinModelPortaliO54728.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057888.

Chemistry databases

SwissLipidsiSLP:000000624.

PTM databases

iPTMnetiO54728.
PhosphoSitePlusiO54728.

Proteomic databases

PaxDbiO54728.
PRIDEiO54728.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192259.
KEGGirno:192259.

Organism-specific databases

CTDi151056.
RGDi621565. Plb1.

Phylogenomic databases

eggNOGiKOG3670. Eukaryota.
ENOG410ZM9P. LUCA.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiO54728.
KOiK14621.
PhylomeDBiO54728.

Miscellaneous databases

PROiO54728.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013830. SGNH_hydro.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
SUPFAMiSSF52266. SSF52266. 7 hits.
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLB1_RAT
AccessioniPrimary (citable) accession number: O54728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.