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O54728

- PLB1_RAT

UniProt

O54728 - PLB1_RAT

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Protein

Phospholipase B1, membrane-associated

Gene

Plb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids.3 Publications

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei404 – 4041NucleophileBy similarity
Binding sitei478 – 4781Substrate; via amide nitrogenBy similarity
Binding sitei517 – 5171SubstrateBy similarity
Active sitei1351 – 13511By similarity
Active sitei1354 – 13541By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: RGD
  2. phospholipase A2 activity Source: RGD
  3. retinyl-palmitate esterase activity Source: RGD

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. phospholipid metabolic process Source: RGD
  3. retinol metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:Plb1
Synonyms:Phlpb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621565. Plb1.

Subcellular locationi

Apical cell membrane 2 Publications; Single-pass type I membrane protein 2 Publications
Note: Present in the intestinal brush border membranes.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 14221399ExtracellularSequence AnalysisAdd
BLAST
Transmembranei1423 – 144321HelicalSequence AnalysisAdd
BLAST
Topological domaini1444 – 14507CytoplasmicSequence Analysis

GO - Cellular componenti

  1. brush border membrane Source: RGD
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi404 – 4041S → A or C: Loss of activity. 1 Publication
Mutagenesisi414 – 4141S → A: Decrease in activity. 1 Publication
Mutagenesisi429 – 4291S → A: Decrease in activity. 1 Publication
Mutagenesisi459 – 4591S → A: Decrease in activity. 1 Publication
Mutagenesisi518 – 5181D → A or V: Loss of activity. 1 Publication
Mutagenesisi518 – 5181D → E or N: Decrease in activity. 1 Publication
Mutagenesisi659 – 6591H → A: Loss of activity. 1 Publication
Mutagenesisi665 – 6651H → A: Slight effect on activity. 1 Publication
Mutagenesisi686 – 6861H → A: No effect on activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 14501427Phospholipase B1, membrane-associatedPRO_0000324386Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi801 – 8011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1059 – 10591N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54728.
PRIDEiO54728.

PTM databases

PhosphoSiteiO54728.

Expressioni

Tissue specificityi

Expressed in the ileum mucosa, Paneth cells spermatocytes, spermatids and sperm (at protein level). Expressed in the ileum, jejunum, esophagus and testis.2 Publications

Gene expression databases

GenevestigatoriO54728.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000057888.

Structurei

3D structure databases

ProteinModelPortaliO54728.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati41 – 3513111Add
BLAST
Repeati366 – 7113462Add
BLAST
Repeati712 – 10583473Add
BLAST
Repeati1068 – 14073404Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 140713674 X 308-326 AA approximate repeatsAdd
BLAST
Regioni1408 – 145043Necessary for membrane localizationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili590 – 61728Sequence AnalysisAdd
BLAST

Domaini

Repeat 2 contains the catalytic domain.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiO54728.
KOiK14621.
PhylomeDBiO54728.

Family and domain databases

Gene3Di3.40.50.1110. 5 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54728-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESWPGVSLV GLLLLLLLGQ GPSQIHGSSG ENTSQPQQVF RTLKNFSFPC
60 70 80 90 100
KPKKLELSVL SKSVHSLRPS DIKLVAAIGN LETPPAPGSG VVNMEKPQSL
110 120 130 140 150
ESELQNVCIG IMTALSDIIR HFNPSVLMPT CSPGKGTAGH TTIAEDLWIQ
160 170 180 190 200
AKELVRHLKD NPELDFEKDW KLITVLFSNT SQCHLCSSDQ QKRHLMKHME
210 220 230 240 250
MLSGVLDYLH REVPRAFVNL VDLSEVLTMA QQHQETGFSP APEICKCSEE
260 270 280 290 300
ITKLSKAVMQ WSYQEAWEDL LASSKFNKHE TFAVVFQSFF SEVELPLERP
310 320 330 340 350
SPQDSTTLAL RIWNSMMEPV GRKDGTLNEA ERKTMKCPSQ ESPYLFTYRN
360 370 380 390 400
SNYQARQLKP IGKFQMKEGT KFTCPDKDPS DSIPTTVHRL RPADIKVIGA
410 420 430 440 450
MGDSLTAGNG AGSSPGNVLD VLTQYRGLSW SVGGDETIET VTTLANILRE
460 470 480 490 500
FNPSLKGFSV GTGKENTPRA SFNQAVAGAK SDGLAAQAKK LVSLMKDDKT
510 520 530 540 550
INFQEDWKII TVFIGGNDLC GSCNNLARFS PQTFTDNIKT ALDILHAEVP
560 570 580 590 600
RAFVNMVSVI EITPLRELFN EPKVSCPRMI LRSLCPCVLN LGENSAELAQ
610 620 630 640 650
LVERNRQYQE ETGKLIESGR YDTRDDFTVV LQPMFENVVM PRTLEGLPDS
660 670 680 690 700
SFFAPDCFHF NVKTHARSAI ALWKNMLEPV GRKTRHQNFE IKVPIMCPNQ
710 720 730 740 750
TSPFLSTTKN SNLGHGTSMS CEEKAPSASP PTSVHTLRPA DIQVVAALGD
760 770 780 790 800
SVTAGNGISS QEGDLADVTT QYRGLSYSAG GDKFLENVTT LPNILREFNG
810 820 830 840 850
NLTGYSVGTG DVNSASAFLN QAVPGAKAEN LASQVQTLIQ KMKNDTRVNF
860 870 880 890 900
HQDWKVITVM IGASDLCDFC KDSNRYSAAN FSDHLRNALD ILHKEVPRAL
910 920 930 940 950
VNLVDFMNPS IIRQVFLKNP DKCPVNQTSV LCNCVLTPGE DSHELARLEA
960 970 980 990 1000
FTKSYQSSML QLVESGRYDT REDFSVVLQP FLFNIRLPIL ENGNPDTSFF
1010 1020 1030 1040 1050
APDCILLSQK FHTQLARALW ANMLEPLGKK MDTLDPKELI ALACPTKDKP
1060 1070 1080 1090 1100
FLRTFRNSNY TYPIKPAIEN WGSDFLCTEQ SPSSKVPTSV HELRPSDIKV
1110 1120 1130 1140 1150
VAAMGDFLTT ATGARPSESS SLDTPWRGLS WSIGGDGTLE THTTLPNILK
1160 1170 1180 1190 1200
KFNPSILGFS TGTLENTAGL NVAEEGARAQ DMPAQAQALV KKMKSTPTIN
1210 1220 1230 1240 1250
IQEDWKLITL LIGNNDLCLY CEDPENYSTR EYVKYIQHAL DIFYEELPRV
1260 1270 1280 1290 1300
FINVVEVMEL SGLLHDQGGK CAMPLAVQKN CSCLKRSQNL MAMQELKKVN
1310 1320 1330 1340 1350
GNLQSALSEL SYWHRYMQRE DFAVTVQPFF RNTFVPLDER GGLDLTFFSE
1360 1370 1380 1390 1400
DCFHFSVRGH AEMAIALWNN MLEPVGKKTT SNNFTYNRTK LKCPSPENPF
1410 1420 1430 1440 1450
LYTVRNSQIL LDKAKENSNT LYWAVPVAAV GGLVVGILGM MLWRTVRLVQ
Length:1,450
Mass (Da):161,089
Last modified:June 1, 1998 - v1
Checksum:i4555898C8FD91F45
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63648 mRNA. Translation: BAA23813.1.
RefSeqiNP_620253.1. NM_138898.1.
UniGeneiRn.91079.

Genome annotation databases

GeneIDi192259.
KEGGirno:192259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D63648 mRNA. Translation: BAA23813.1 .
RefSeqi NP_620253.1. NM_138898.1.
UniGenei Rn.91079.

3D structure databases

ProteinModelPortali O54728.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000057888.

PTM databases

PhosphoSitei O54728.

Proteomic databases

PaxDbi O54728.
PRIDEi O54728.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 192259.
KEGGi rno:192259.

Organism-specific databases

CTDi 151056.
RGDi 621565. Plb1.

Phylogenomic databases

eggNOGi NOG311176.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi O54728.
KOi K14621.
PhylomeDBi O54728.

Miscellaneous databases

NextBioi 622930.
PROi O54728.

Gene expression databases

Genevestigatori O54728.

Family and domain databases

Gene3Di 3.40.50.1110. 5 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of functional domains of rat intestinal phospholipase B/lipase. Its cDNA cloning, expression, and tissue distribution."
    Takemori H., Zolotaryov F.N., Ting L., Urbain T., Komatsubara T., Hatano O., Okamoto M., Tojo H.
    J. Biol. Chem. 273:2222-2231(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PROTEOLYTIC PROCESSING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Ileal mucosa.
  2. "Purification and characterization of a catalytic domain of rat intestinal phospholipase B/lipase associated with brush border membranes."
    Tojo H., Ichida T., Okamoto M.
    J. Biol. Chem. 273:2214-2221(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 368-380 AND 529-559, FUNCTION, SUBCELLULAR LOCATION.
  3. "Further characterization of a novel phospholipase B (phospholipase A2-lysophospholipase) from intestinal brush-border membranes."
    Pind S., Kuksis A.
    Biochem. Cell Biol. 69:346-357(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. "Identification of essential residues for catalysis of rat intestinal phospholipase B/lipase."
    Lu T., Ito M., Tchoua U., Takemori H., Okamoto M., Tojo H.
    Biochemistry 40:7133-7139(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-404; SER-414; SER-429; SER-459; ASP-518; HIS-659; HIS-665 AND HIS-686.

Entry informationi

Entry nameiPLB1_RAT
AccessioniPrimary (citable) accession number: O54728
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3