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Protein

Polymerase I and transcript release factor

Gene

Ptrf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in caveolae formation and organization. Required for the sequestration of mobile caveolin into immobile caveolae. Termination of transcription by RNA polymerase I involves pausing of transcription by TTF1, and the dissociation of the transcription complex, releasing pre-rRNA and RNA polymerase I from the template. PTRF is required for dissociation of the ternary transcription complex.5 Publications

GO - Molecular functioni

  • poly(A) RNA binding Source: MGI
  • rRNA primary transcript binding Source: UniProtKB

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • termination of RNA polymerase I transcription Source: UniProtKB
  • transcription initiation from RNA polymerase I promoter Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-73863. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase I and transcript release factor
Alternative name(s):
Cav-p60
Cavin-1
Gene namesi
Name:PtrfImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1277968. Ptrf.

Subcellular locationi

GO - Cellular componenti

  • caveola Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • cytosol Source: UniProtKB-SubCell
  • endoplasmic reticulum Source: UniProtKB-SubCell
  • membrane raft Source: MGI
  • mitochondrion Source: UniProtKB
  • nucleus Source: UniProtKB
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show a metabolic phenotype of significantly reduced adipose tissue mass, higher circulating triglyceride levels, glucose intolerance, and hyperinsulinemia, consistent with a lipodystrophy. Cells from various tissues, including lung epithelium, intestinal smooth muscle, skeletal muscle, and endothelial cells showed no detectable caveolae cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000970951 – 392Polymerase I and transcript release factorAdd BLAST392

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei21PhosphoserineBy similarity1
Modified residuei38PhosphoserineCombined sources1
Modified residuei40PhosphothreonineCombined sources1
Modified residuei42PhosphoserineCombined sources1
Modified residuei48PhosphoserineBy similarity1
Cross-linki118Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei120PhosphoserineBy similarity1
Modified residuei158PhosphotyrosineBy similarity1
Cross-linki163Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki163Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei169PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei173PhosphoserineCombined sources1
Modified residuei177PhosphoserineBy similarity1
Modified residuei198PhosphothreonineCombined sources1
Modified residuei204PhosphoserineCombined sources1
Modified residuei205PhosphoserineCombined sources1
Modified residuei302PhosphoserineCombined sources1
Modified residuei304PhosphothreonineBy similarity1
Modified residuei310PhosphotyrosineCombined sources1
Modified residuei367PhosphoserineBy similarity1
Modified residuei368PhosphoserineBy similarity1
Modified residuei381PhosphoserineBy similarity1
Modified residuei389PhosphoserineBy similarity1
Modified residuei391PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated. Present in active and inactive forms. Changes in phosphorylation pattern may alter activity.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO54724.
PaxDbiO54724.
PeptideAtlasiO54724.
PRIDEiO54724.

PTM databases

iPTMnetiO54724.
PhosphoSitePlusiO54724.

Expressioni

Tissue specificityi

Expressed in all tissues tested (lung, heart, testis, kidney, muscle, liver, spleen and brain). Highest levels in lung and heart.2 Publications

Gene expression databases

BgeeiENSMUSG00000004044.
CleanExiMM_PTRF.
GenevisibleiO54724. MM.

Interactioni

Subunit structurei

Interacts with LIPE in the adipocyte cytoplasm (By similarity). Interacts with RNA polymerase I and TTF1. Binds the 3' end of pre-rRNA. Interacts with transcription factor ZNF148.By similarity2 Publications

Protein-protein interaction databases

BioGridi202511. 3 interactors.
DIPiDIP-40506N.
IntActiO54724. 9 interactors.
MINTiMINT-1549738.
STRINGi10090.ENSMUSP00000058321.

Structurei

Secondary structure

1392
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi49 – 116Combined sources68
Helixi119 – 144Combined sources26

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QKVX-ray3.00A/B/C45-155[»]
ProteinModelPortaliO54724.
SMRiO54724.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili201 – 284Sequence analysisAdd BLAST84

Sequence similaritiesi

Belongs to the PTRF/SDPR family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IV8W. Eukaryota.
ENOG410XT47. LUCA.
GeneTreeiENSGT00530000063058.
HOGENOMiHOG000293135.
HOVERGENiHBG056807.
InParanoidiO54724.
KOiK19387.
OMAiHTIYARS.
OrthoDBiEOG091G09Y1.
PhylomeDBiO54724.
TreeFamiTF331031.

Family and domain databases

InterProiIPR026752. Cavin_fam.
IPR033297. PTRF.
[Graphical view]
PANTHERiPTHR15240. PTHR15240. 2 hits.
PTHR15240:SF3. PTHR15240:SF3. 2 hits.
PfamiPF15237. PTRF_SDPR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDVTLHIVE RPYSGFPDAS SEGPEPTQGE ARATEEPSGT GSDELIKSDQ
60 70 80 90 100
VNGVLVLSLL DKIIGAVDQI QLTQAQLEER QAEMEGAVQS IQGELSKLGK
110 120 130 140 150
AHATTSNTVS KLLEKVRKVS VNVKTVRGSL ERQAGQIKKL EVNEAELLRR
160 170 180 190 200
RNFKVMIYQD EVKLPAKLSV SKSLKESEAL PEKEGDELGE GERPEDDTAA
210 220 230 240 250
IELSSDEAVE VEEVIEESRA ERIKRSGLRR VDDFKKAFSK EKMEKTKVRT
260 270 280 290 300
RENLEKTRLK TKENLEKTRH TLEKRMNKLG TRLVPVERRE KLKTSRDKLR
310 320 330 340 350
KSFTPDHVVY ARSKTAVYKV PPFTFHVKKI REGEVEVLKA TEMVEVGPED
360 370 380 390
DEVGAERGEA TDLLRGSSPD VHTLLEITEE SDAVLVDKSD SD
Length:392
Mass (Da):43,954
Last modified:June 1, 1998 - v1
Checksum:i6871CBC06DCF5C35
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036249 mRNA. Translation: AAC53588.1.
AF458959 Genomic DNA. Translation: AAL60240.1.
AL591466 Genomic DNA. Translation: CAM19459.1.
BC110698 mRNA. Translation: AAI10699.1.
BC117527 mRNA. Translation: AAI17528.1.
BC116701 mRNA. Translation: AAI16702.1.
CCDSiCCDS25442.1.
RefSeqiNP_033012.1. NM_008986.2.
UniGeneiMm.21864.

Genome annotation databases

EnsembliENSMUST00000060792; ENSMUSP00000058321; ENSMUSG00000004044.
GeneIDi19285.
KEGGimmu:19285.
UCSCiuc007lmr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036249 mRNA. Translation: AAC53588.1.
AF458959 Genomic DNA. Translation: AAL60240.1.
AL591466 Genomic DNA. Translation: CAM19459.1.
BC110698 mRNA. Translation: AAI10699.1.
BC117527 mRNA. Translation: AAI17528.1.
BC116701 mRNA. Translation: AAI16702.1.
CCDSiCCDS25442.1.
RefSeqiNP_033012.1. NM_008986.2.
UniGeneiMm.21864.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4QKVX-ray3.00A/B/C45-155[»]
ProteinModelPortaliO54724.
SMRiO54724.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202511. 3 interactors.
DIPiDIP-40506N.
IntActiO54724. 9 interactors.
MINTiMINT-1549738.
STRINGi10090.ENSMUSP00000058321.

PTM databases

iPTMnetiO54724.
PhosphoSitePlusiO54724.

Proteomic databases

MaxQBiO54724.
PaxDbiO54724.
PeptideAtlasiO54724.
PRIDEiO54724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060792; ENSMUSP00000058321; ENSMUSG00000004044.
GeneIDi19285.
KEGGimmu:19285.
UCSCiuc007lmr.2. mouse.

Organism-specific databases

CTDi284119.
MGIiMGI:1277968. Ptrf.

Phylogenomic databases

eggNOGiENOG410IV8W. Eukaryota.
ENOG410XT47. LUCA.
GeneTreeiENSGT00530000063058.
HOGENOMiHOG000293135.
HOVERGENiHBG056807.
InParanoidiO54724.
KOiK19387.
OMAiHTIYARS.
OrthoDBiEOG091G09Y1.
PhylomeDBiO54724.
TreeFamiTF331031.

Enzyme and pathway databases

ReactomeiR-MMU-73863. RNA Polymerase I Transcription Termination.

Miscellaneous databases

ChiTaRSiPtrf. mouse.
PROiO54724.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004044.
CleanExiMM_PTRF.
GenevisibleiO54724. MM.

Family and domain databases

InterProiIPR026752. Cavin_fam.
IPR033297. PTRF.
[Graphical view]
PANTHERiPTHR15240. PTHR15240. 2 hits.
PTHR15240:SF3. PTHR15240:SF3. 2 hits.
PfamiPF15237. PTRF_SDPR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTRF_MOUSE
AccessioniPrimary (citable) accession number: O54724
Secondary accession number(s): Q2TAW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.