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Protein

E3 SUMO-protein ligase PIAS3

Gene

Pias3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Repressor of STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Repressor of MITF transcriptional activity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1 (By similarity). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (By similarity).By similarity3 Publications

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri312 – 38978SP-RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3232118. SUMOylation of transcription factors.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5696395. Formation of Incision Complex in GG-NER.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 SUMO-protein ligase PIAS3 (EC:6.3.2.-)
Alternative name(s):
Protein inhibitor of activated STAT protein 3
Gene namesi
Name:Pias3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913126. Pias3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • dendrite Source: Ensembl
  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
  • synapse Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi19 – 235LQVLL → AQVAA: Nuclear and cytoplasmic location. 1 Publication
Mutagenesisi141 – 1411L → A: Greatly reduced interaction with STAT3.
Mutagenesisi143 – 1431R → N: Abolishes interaction with STAT3.
Mutagenesisi143 – 1431R → Q: Abolishes interaction with STAT3.
Mutagenesisi242 – 2443INI → SDS: Nuclear and cytoplasmic location. 1 Publication
Mutagenesisi343 – 3486CAHLQS → GADLQG: Nuclear and cytoplasmic location. 1 Publication
Mutagenesisi343 – 3431C → S: Loss of promotion of IRF1 sumoylation, as well as of autosumoylation; partial loss of suppression of IRF1 transcriptional activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 628627E3 SUMO-protein ligase PIAS3PRO_0000218980Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Cross-linki46 – 46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki230 – 230Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei295 – 2951Asymmetric dimethylarginineBy similarity
Cross-linki307 – 307Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei455 – 4551PhosphoserineBy similarity
Modified residuei456 – 4561PhosphoserineBy similarity
Modified residuei472 – 4721PhosphoserineBy similarity
Modified residuei492 – 4921PhosphoserineBy similarity

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO54714.
PaxDbiO54714.
PRIDEiO54714.

PTM databases

PhosphoSiteiO54714.

Expressioni

Tissue specificityi

Expressed in kidney, heart, spleen, brain and cerebellum; weak expression, if any, in liver and lung.2 Publications

Developmental stagei

Expressed as early as 7.6 dpc. Expression remains high through 15.5 dpc.1 Publication

Gene expression databases

BgeeiENSMUSG00000028101.
CleanExiMM_PIAS3.
ExpressionAtlasiO54714. baseline and differential.
GenevisibleiO54714. MM.

Interactioni

Subunit structurei

Monomer. Interacts with PLAG1 and ZFHX3. Interacts with STAT5A; the interaction occurs on stimulation by PRL (By similarity). Binds SUMO1 and UBE2I. Interacts with AR, BCL11A, HMGA2, IRF1 and NCOA2. Interacts with MITF; the interaction inhibits the transcriptional activity of MITF. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3-mediated transcriptional activity. Interacts with MTA1. Interacts with CCAR2 (via N-terminus) (By similarity).By similarity9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Gfi1P703386EBI-927969,EBI-3954754

GO - Molecular functioni

Protein-protein interaction databases

BioGridi230874. 8 interactions.
IntActiO54714. 2 interactions.
MINTiMINT-246603.
STRINGi10090.ENSMUSP00000069259.

Structurei

3D structure databases

ProteinModelPortaliO54714.
SMRiO54714. Positions 1-65, 126-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 4535SAPPROSITE-ProRule annotationAdd
BLAST
Domaini115 – 280166PINITPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 200199Interaction with CCAR2By similarityAdd
BLAST
Regioni450 – 46011SUMO1-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi19 – 235LXXLL motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi81 – 13353Pro-richAdd
BLAST

Domaini

The LXXLL motif is a transcriptional coregulator signature.

Sequence similaritiesi

Belongs to the PIAS family.Curated
Contains 1 PINIT domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation
Contains 1 SP-RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri312 – 38978SP-RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2169. Eukaryota.
ENOG410XQ2E. LUCA.
GeneTreeiENSGT00550000074410.
HOGENOMiHOG000230594.
HOVERGENiHBG053598.
InParanoidiO54714.
KOiK16064.
OMAiGELKHMV.
OrthoDBiEOG091G08G5.
PhylomeDBiO54714.
TreeFamiTF323787.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR027226. PIAS3.
IPR023321. PINIT.
IPR003034. SAP_dom.
IPR004181. Znf_MIZ.
[Graphical view]
PANTHERiPTHR10782:SF10. PTHR10782:SF10. 2 hits.
PfamiPF14324. PINIT. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54714-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA
60 70 80 90 100
PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL
110 120 130 140 150
LTPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST
160 170 180 190 200
SSQRFEEAHF TFALTPQQLQ QILTSREVLP GAKCDYTIQV QLRFCLCETS
210 220 230 240 250
CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL
260 270 280 290 300
SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS
310 320 330 340 350
RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD
360 370 380 390 400
AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILNS CSDCDEIQFM
410 420 430 440 450
EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SAVQEGIQPE SKKRVEVIDL
460 470 480 490 500
TIESSSDEED LPPTKKHCPV TSAAIPALPG SKGALTSGHQ PSSVLRSPAM
510 520 530 540 550
GTLGSDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL
560 570 580 590 600
DEQDTLGHFF QYRGTPSHFL GPLAPTLGSS HRSSTPAPPP GRVSSIVAPG
610 620
SSLREGHGGP LPSGPSLTGC RSDVISLD
Length:628
Mass (Da):68,318
Last modified:December 7, 2004 - v3
Checksum:i2827F52715F0E549
GO
Isoform 2 (identifier: O54714-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: Missing.

Show »
Length:619
Mass (Da):67,309
Checksum:iF368B209502A47F3
GO
Isoform 3 (identifier: O54714-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     87-121: Missing.

Show »
Length:593
Mass (Da):64,741
Checksum:i651B188168468F47
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 99Missing in isoform 2. 2 PublicationsVSP_012203
Alternative sequencei87 – 12135Missing in isoform 3. 1 PublicationVSP_012204Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040619 mRNA. Translation: BAC30647.1.
AK077551 mRNA. Translation: BAC36858.1.
BC023128 mRNA. Translation: AAH23128.1.
BC051252 mRNA. Translation: AAH51252.1.
AF034080 mRNA. Translation: AAB88902.3.
CCDSiCCDS17645.2. [O54714-3]
CCDS38559.1. [O54714-2]
CCDS51007.1. [O54714-1]
RefSeqiNP_001159421.1. NM_001165949.1. [O54714-1]
NP_061282.2. NM_018812.2. [O54714-3]
NP_666247.1. NM_146135.2. [O54714-2]
XP_006501456.1. XM_006501393.1. [O54714-2]
UniGeneiMm.1635.

Genome annotation databases

EnsembliENSMUST00000064900; ENSMUSP00000069259; ENSMUSG00000028101. [O54714-1]
ENSMUST00000107076; ENSMUSP00000102691; ENSMUSG00000028101. [O54714-2]
ENSMUST00000107077; ENSMUSP00000102692; ENSMUSG00000028101. [O54714-3]
GeneIDi229615.
KEGGimmu:229615.
UCSCiuc008qnt.2. mouse. [O54714-1]
uc008qnu.2. mouse. [O54714-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK040619 mRNA. Translation: BAC30647.1.
AK077551 mRNA. Translation: BAC36858.1.
BC023128 mRNA. Translation: AAH23128.1.
BC051252 mRNA. Translation: AAH51252.1.
AF034080 mRNA. Translation: AAB88902.3.
CCDSiCCDS17645.2. [O54714-3]
CCDS38559.1. [O54714-2]
CCDS51007.1. [O54714-1]
RefSeqiNP_001159421.1. NM_001165949.1. [O54714-1]
NP_061282.2. NM_018812.2. [O54714-3]
NP_666247.1. NM_146135.2. [O54714-2]
XP_006501456.1. XM_006501393.1. [O54714-2]
UniGeneiMm.1635.

3D structure databases

ProteinModelPortaliO54714.
SMRiO54714. Positions 1-65, 126-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230874. 8 interactions.
IntActiO54714. 2 interactions.
MINTiMINT-246603.
STRINGi10090.ENSMUSP00000069259.

PTM databases

PhosphoSiteiO54714.

Proteomic databases

MaxQBiO54714.
PaxDbiO54714.
PRIDEiO54714.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064900; ENSMUSP00000069259; ENSMUSG00000028101. [O54714-1]
ENSMUST00000107076; ENSMUSP00000102691; ENSMUSG00000028101. [O54714-2]
ENSMUST00000107077; ENSMUSP00000102692; ENSMUSG00000028101. [O54714-3]
GeneIDi229615.
KEGGimmu:229615.
UCSCiuc008qnt.2. mouse. [O54714-1]
uc008qnu.2. mouse. [O54714-3]

Organism-specific databases

CTDi10401.
MGIiMGI:1913126. Pias3.

Phylogenomic databases

eggNOGiKOG2169. Eukaryota.
ENOG410XQ2E. LUCA.
GeneTreeiENSGT00550000074410.
HOGENOMiHOG000230594.
HOVERGENiHBG053598.
InParanoidiO54714.
KOiK16064.
OMAiGELKHMV.
OrthoDBiEOG091G08G5.
PhylomeDBiO54714.
TreeFamiTF323787.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiR-MMU-3232118. SUMOylation of transcription factors.
R-MMU-4615885. SUMOylation of DNA replication proteins.
R-MMU-5696395. Formation of Incision Complex in GG-NER.

Miscellaneous databases

ChiTaRSiPias3. mouse.
PROiO54714.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028101.
CleanExiMM_PIAS3.
ExpressionAtlasiO54714. baseline and differential.
GenevisibleiO54714. MM.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
InterProiIPR027226. PIAS3.
IPR023321. PINIT.
IPR003034. SAP_dom.
IPR004181. Znf_MIZ.
[Graphical view]
PANTHERiPTHR10782:SF10. PTHR10782:SF10. 2 hits.
PfamiPF14324. PINIT. 1 hit.
PF02891. zf-MIZ. 1 hit.
[Graphical view]
SMARTiSM00513. SAP. 1 hit.
[Graphical view]
PROSITEiPS51466. PINIT. 1 hit.
PS50800. SAP. 1 hit.
PS51044. ZF_SP_RING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIAS3_MOUSE
AccessioniPrimary (citable) accession number: O54714
Secondary accession number(s): Q80WF8, Q8R598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: December 7, 2004
Last modified: September 7, 2016
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.