Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O54705

- NOS2_CAVPO

UniProt

O54705 - NOS2_CAVPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 (By similarity).By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Protein has several cofactor binding sites:
  • hemeBy similarity
  • FADBy similarityNote: Binds 1 FAD.By similarity
  • FMNBy similarityNote: Binds 1 FMN.By similarity
  • 5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity

Enzyme regulationi

Regulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991Iron (heme axial ligand)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi622 – 65332FMNPROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi766 – 77712FADBy similarityAdd
BLAST
Nucleotide bindingi902 – 91211FADBy similarityAdd
BLAST
Nucleotide bindingi977 – 99519NADPBy similarityAdd
BLAST
Nucleotide bindingi1075 – 109016NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. defense response to bacterium Source: UniProtKB
  2. nitric oxide biosynthetic process Source: InterPro
  3. peptidyl-cysteine S-nitrosylation Source: UniProtKB
  4. superoxide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
Synonyms:NOS
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11491149Nitric oxide synthase, induciblePRO_0000170929Add
BLAST

Expressioni

Tissue specificityi

Expressed in the lung and colon. Not detected in the heart, aorta, liver, kidney, and spleen.

Inductioni

By lipopolysaccharide (LPS); in kidney, spleen, and colon. Expression is reduced in the presence of LPS in lung.

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000017277.

Structurei

3D structure databases

ProteinModelPortaliO54705.
SMRiO54705. Positions 82-501, 702-1129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini538 – 676139Flavodoxin-likePROSITE-ProRule annotationAdd
BLAST
Domaini729 – 969241FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni508 – 52821Calmodulin-bindingSequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
HOVERGENiHBG000159.
InParanoidiO54705.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54705-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MACPWNFLWK LKSSRYDLTE EKDINNNVGK ASHLYSPEIQ DDPKYCSPGK
60 70 80 90 100
HQNGSSQSLT GTAKKVPESQ SKPHKPSPTC SQHMKIKNWG NGMILQDTLH
110 120 130 140 150
TKAKTNFTCK PKSCLGSVMN PRSMTRGPRD TPIPPDELLP QAIEFVNQYY
160 170 180 190 200
DSFKEAKIEE YLARVETVTK EIETTGTYQL TGDELIFATK LAWRNAPRCI
210 220 230 240 250
GRIQWSNLQV FDARSCHTAQ EMFEHICRHV RYSTNNGNIR SAITVFPQRT
260 270 280 290 300
DGKHDFRVWN AQLIRYAGYQ MPDGTIQGDP ANLEFTQLCI DLGWKPRYGR
310 320 330 340 350
FDVLPLILQA DGRDPELFEI PPDLVLEVPM EHPKYEWFQD LGLKWYALPA
360 370 380 390 400
VANMLLEVGG LEFPACPFNG WYMGTEIGVR DFCDAQRYNI LEEVGRRMGL
410 420 430 440 450
ETHTLASLWK DRAVTEINVA VLHSFQKQNV TIMDHHSAAE SFMKHMQNEY
460 470 480 490 500
RARGGCPADW IWLVPPISGS ITPVFHQEML NYILSPFYYY QVEAWKTHVW
510 520 530 540 550
QDETRRPKRR EIPFRVLAKA TLFASLLMRK MMASRVRATI LFATETGKSE
560 570 580 590 600
ALAQDLGALF SCAFNPKVLC MDQYQLSSLE EEKLLLVVTS TFGNGDCPGN
610 620 630 640 650
GETLKKSLFV LKKLTNTFRY AVFGLGSSMY PRFCAFAHDI DIKLSQLGAS
660 670 680 690 700
QLTPVGEGDE LSGQEDAFCT WAVQTFQAAC AAFDVRGRHH ITIPKRYTSS
710 720 730 740 750
VTWEPYHYRL VQDSQPLDLN KALSRMHATD VFTMRLKSQK NLQSPKSSRT
760 770 780 790 800
TLLMELSCDD SRSLAYLPGE HLGVFPCNQP ALVQGILECV VDNPGPHHTV
810 820 830 840 850
CLEVLDDSGS YWAKDKRLPP CSLSQALTYF LDITTPPTQL QLQKLARLAT
860 870 880 890 900
EQAERLRLES LSQPSEYNKW KFTNSPTFLE VLEEFPSLRV PAAFLLSQLP
910 920 930 940 950
ILKPRYYSIS SSLDHTPAEV HLTVAVVTYR TRDGRGPLHH GVCSTWFSGL
960 970 980 990 1000
KPQDPVPCLV RSVNSFQLPK DPSQPCILIG PGTGIAPFRS FWQQRLHNLK
1010 1020 1030 1040 1050
HTGLQGGRMT LLFGCRHPEE DHIYKEEMQE MVQKGVLHEV HTAYSRLPGK
1060 1070 1080 1090 1100
PKAYVQDILR QQLAREVLRV LHEEPGHLYV CGNVLMAQDV ACTLKQLLAA
1110 1120 1130 1140
KLNLNEEQVE DYFFQLKSQK RYHEDIFGAV FPHGVKKDRA ERPPGDDKL
Length:1,149
Mass (Da):130,632
Last modified:June 1, 1998 - v1
Checksum:i66D595A2486DB50E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027180 mRNA. Translation: AAC33177.1.
RefSeqiNP_001166455.1. NM_001172984.1.

Genome annotation databases

GeneIDi100135576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF027180 mRNA. Translation: AAC33177.1 .
RefSeqi NP_001166455.1. NM_001172984.1.

3D structure databases

ProteinModelPortali O54705.
SMRi O54705. Positions 82-501, 702-1129.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000017277.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100135576.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
HOVERGENi HBG000159.
InParanoidi O54705.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of Ca2+-dependent inducible nitric oxide synthase from guinea-pig lung."
    Shirato M., Sakamoto T., Uchida Y., Nomura A., Ishii Y., Iijima H., Goto Y., Hasegawa S.
    Biochem. J. 333:795-799(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hartley.
    Tissue: Lung.

Entry informationi

Entry nameiNOS2_CAVPO
AccessioniPrimary (citable) accession number: O54705
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3