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O54705 (NOS2_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:NOS2
Synonyms:NOS
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length1149 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Regulated by calcium/calmodulin.

Subunit structure

Homodimer. Binds SLC9A3R1 By similarity.

Tissue specificity

Expressed in the lung and colon. Not detected in the heart, aorta, liver, kidney, and spleen.

Induction

By lipopolysaccharide (LPS); in kidney, spleen, and colon. Expression is reduced in the presence of LPS in lung.

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11491149Nitric oxide synthase, inducible
PRO_0000170929

Regions

Domain538 – 676139Flavodoxin-like
Domain729 – 969241FAD-binding FR-type
Nucleotide binding622 – 65332FMN By similarity
Nucleotide binding766 – 77712FAD By similarity
Nucleotide binding902 – 91211FAD By similarity
Nucleotide binding977 – 99519NADP By similarity
Nucleotide binding1075 – 109016NADP By similarity
Region508 – 52821Calmodulin-binding Potential

Sites

Metal binding1991Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
O54705 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 66D595A2486DB50E

FASTA1,149130,632
        10         20         30         40         50         60 
MACPWNFLWK LKSSRYDLTE EKDINNNVGK ASHLYSPEIQ DDPKYCSPGK HQNGSSQSLT 

        70         80         90        100        110        120 
GTAKKVPESQ SKPHKPSPTC SQHMKIKNWG NGMILQDTLH TKAKTNFTCK PKSCLGSVMN 

       130        140        150        160        170        180 
PRSMTRGPRD TPIPPDELLP QAIEFVNQYY DSFKEAKIEE YLARVETVTK EIETTGTYQL 

       190        200        210        220        230        240 
TGDELIFATK LAWRNAPRCI GRIQWSNLQV FDARSCHTAQ EMFEHICRHV RYSTNNGNIR 

       250        260        270        280        290        300 
SAITVFPQRT DGKHDFRVWN AQLIRYAGYQ MPDGTIQGDP ANLEFTQLCI DLGWKPRYGR 

       310        320        330        340        350        360 
FDVLPLILQA DGRDPELFEI PPDLVLEVPM EHPKYEWFQD LGLKWYALPA VANMLLEVGG 

       370        380        390        400        410        420 
LEFPACPFNG WYMGTEIGVR DFCDAQRYNI LEEVGRRMGL ETHTLASLWK DRAVTEINVA 

       430        440        450        460        470        480 
VLHSFQKQNV TIMDHHSAAE SFMKHMQNEY RARGGCPADW IWLVPPISGS ITPVFHQEML 

       490        500        510        520        530        540 
NYILSPFYYY QVEAWKTHVW QDETRRPKRR EIPFRVLAKA TLFASLLMRK MMASRVRATI 

       550        560        570        580        590        600 
LFATETGKSE ALAQDLGALF SCAFNPKVLC MDQYQLSSLE EEKLLLVVTS TFGNGDCPGN 

       610        620        630        640        650        660 
GETLKKSLFV LKKLTNTFRY AVFGLGSSMY PRFCAFAHDI DIKLSQLGAS QLTPVGEGDE 

       670        680        690        700        710        720 
LSGQEDAFCT WAVQTFQAAC AAFDVRGRHH ITIPKRYTSS VTWEPYHYRL VQDSQPLDLN 

       730        740        750        760        770        780 
KALSRMHATD VFTMRLKSQK NLQSPKSSRT TLLMELSCDD SRSLAYLPGE HLGVFPCNQP 

       790        800        810        820        830        840 
ALVQGILECV VDNPGPHHTV CLEVLDDSGS YWAKDKRLPP CSLSQALTYF LDITTPPTQL 

       850        860        870        880        890        900 
QLQKLARLAT EQAERLRLES LSQPSEYNKW KFTNSPTFLE VLEEFPSLRV PAAFLLSQLP 

       910        920        930        940        950        960 
ILKPRYYSIS SSLDHTPAEV HLTVAVVTYR TRDGRGPLHH GVCSTWFSGL KPQDPVPCLV 

       970        980        990       1000       1010       1020 
RSVNSFQLPK DPSQPCILIG PGTGIAPFRS FWQQRLHNLK HTGLQGGRMT LLFGCRHPEE 

      1030       1040       1050       1060       1070       1080 
DHIYKEEMQE MVQKGVLHEV HTAYSRLPGK PKAYVQDILR QQLAREVLRV LHEEPGHLYV 

      1090       1100       1110       1120       1130       1140 
CGNVLMAQDV ACTLKQLLAA KLNLNEEQVE DYFFQLKSQK RYHEDIFGAV FPHGVKKDRA 


ERPPGDDKL 

« Hide

References

[1]"Molecular cloning and characterization of Ca2+-dependent inducible nitric oxide synthase from guinea-pig lung."
Shirato M., Sakamoto T., Uchida Y., Nomura A., Ishii Y., Iijima H., Goto Y., Hasegawa S.
Biochem. J. 333:795-799(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Hartley.
Tissue: Lung.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF027180 mRNA. Translation: AAC33177.1.
RefSeqNP_001166455.1. NM_001172984.1.

3D structure databases

ProteinModelPortalO54705.
SMRO54705. Positions 82-501, 702-1129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000017277.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100135576.

Organism-specific databases

CTD4843.

Phylogenomic databases

eggNOGCOG4362.
HOGENOMHOG000220884.
HOVERGENHBG000159.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR004030. NO_synthase_oxygenase_dom.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF000333. NOS. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS2_CAVPO
AccessionPrimary (citable) accession number: O54705
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families