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O54705

- NOS2_CAVPO

UniProt

O54705 - NOS2_CAVPO

Protein

Nitric oxide synthase, inducible

Gene

NOS2

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.By similarity

    Catalytic activityi

    2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

    Cofactori

    Heme group.By similarity
    Binds 1 FAD.By similarity
    Binds 1 FMN.By similarity
    Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

    Enzyme regulationi

    Regulated by calcium/calmodulin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi199 – 1991Iron (heme axial ligand)By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi622 – 65332FMNPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi766 – 77712FADBy similarityAdd
    BLAST
    Nucleotide bindingi902 – 91211FADBy similarityAdd
    BLAST
    Nucleotide bindingi977 – 99519NADPBy similarityAdd
    BLAST
    Nucleotide bindingi1075 – 109016NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro
    5. NADP binding Source: InterPro
    6. nitric-oxide synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response to bacterium Source: UniProtKB
    2. nitric oxide biosynthetic process Source: InterPro
    3. peptidyl-cysteine S-nitrosylation Source: UniProtKB
    4. superoxide metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitric oxide synthase, inducible (EC:1.14.13.39)
    Alternative name(s):
    Inducible NO synthase
    Short name:
    Inducible NOS
    Short name:
    iNOS
    NOS type II
    Peptidyl-cysteine S-nitrosylase NOS2
    Gene namesi
    Name:NOS2
    Synonyms:NOS
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11491149Nitric oxide synthase, induciblePRO_0000170929Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in the lung and colon. Not detected in the heart, aorta, liver, kidney, and spleen.

    Inductioni

    By lipopolysaccharide (LPS); in kidney, spleen, and colon. Expression is reduced in the presence of LPS in lung.

    Interactioni

    Subunit structurei

    Homodimer. Binds SLC9A3R1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000017277.

    Structurei

    3D structure databases

    ProteinModelPortaliO54705.
    SMRiO54705. Positions 82-501, 702-1129.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini538 – 676139Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini729 – 969241FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni508 – 52821Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NOS family.Curated
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG4362.
    HOGENOMiHOG000220884.
    HOVERGENiHBG000159.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    3.90.340.10. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000333. NOS. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54705-1 [UniParc]FASTAAdd to Basket

    « Hide

    MACPWNFLWK LKSSRYDLTE EKDINNNVGK ASHLYSPEIQ DDPKYCSPGK     50
    HQNGSSQSLT GTAKKVPESQ SKPHKPSPTC SQHMKIKNWG NGMILQDTLH 100
    TKAKTNFTCK PKSCLGSVMN PRSMTRGPRD TPIPPDELLP QAIEFVNQYY 150
    DSFKEAKIEE YLARVETVTK EIETTGTYQL TGDELIFATK LAWRNAPRCI 200
    GRIQWSNLQV FDARSCHTAQ EMFEHICRHV RYSTNNGNIR SAITVFPQRT 250
    DGKHDFRVWN AQLIRYAGYQ MPDGTIQGDP ANLEFTQLCI DLGWKPRYGR 300
    FDVLPLILQA DGRDPELFEI PPDLVLEVPM EHPKYEWFQD LGLKWYALPA 350
    VANMLLEVGG LEFPACPFNG WYMGTEIGVR DFCDAQRYNI LEEVGRRMGL 400
    ETHTLASLWK DRAVTEINVA VLHSFQKQNV TIMDHHSAAE SFMKHMQNEY 450
    RARGGCPADW IWLVPPISGS ITPVFHQEML NYILSPFYYY QVEAWKTHVW 500
    QDETRRPKRR EIPFRVLAKA TLFASLLMRK MMASRVRATI LFATETGKSE 550
    ALAQDLGALF SCAFNPKVLC MDQYQLSSLE EEKLLLVVTS TFGNGDCPGN 600
    GETLKKSLFV LKKLTNTFRY AVFGLGSSMY PRFCAFAHDI DIKLSQLGAS 650
    QLTPVGEGDE LSGQEDAFCT WAVQTFQAAC AAFDVRGRHH ITIPKRYTSS 700
    VTWEPYHYRL VQDSQPLDLN KALSRMHATD VFTMRLKSQK NLQSPKSSRT 750
    TLLMELSCDD SRSLAYLPGE HLGVFPCNQP ALVQGILECV VDNPGPHHTV 800
    CLEVLDDSGS YWAKDKRLPP CSLSQALTYF LDITTPPTQL QLQKLARLAT 850
    EQAERLRLES LSQPSEYNKW KFTNSPTFLE VLEEFPSLRV PAAFLLSQLP 900
    ILKPRYYSIS SSLDHTPAEV HLTVAVVTYR TRDGRGPLHH GVCSTWFSGL 950
    KPQDPVPCLV RSVNSFQLPK DPSQPCILIG PGTGIAPFRS FWQQRLHNLK 1000
    HTGLQGGRMT LLFGCRHPEE DHIYKEEMQE MVQKGVLHEV HTAYSRLPGK 1050
    PKAYVQDILR QQLAREVLRV LHEEPGHLYV CGNVLMAQDV ACTLKQLLAA 1100
    KLNLNEEQVE DYFFQLKSQK RYHEDIFGAV FPHGVKKDRA ERPPGDDKL 1149
    Length:1,149
    Mass (Da):130,632
    Last modified:June 1, 1998 - v1
    Checksum:i66D595A2486DB50E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF027180 mRNA. Translation: AAC33177.1.
    RefSeqiNP_001166455.1. NM_001172984.1.

    Genome annotation databases

    GeneIDi100135576.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF027180 mRNA. Translation: AAC33177.1 .
    RefSeqi NP_001166455.1. NM_001172984.1.

    3D structure databases

    ProteinModelPortali O54705.
    SMRi O54705. Positions 82-501, 702-1129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000017277.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100135576.

    Organism-specific databases

    CTDi 4843.

    Phylogenomic databases

    eggNOGi COG4362.
    HOGENOMi HOG000220884.
    HOVERGENi HBG000159.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    3.90.340.10. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR012144. NOS_euk.
    IPR004030. NOS_N.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    PF02898. NO_synthase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000333. NOS. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF56512. SSF56512. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    PS60001. NOS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of Ca2+-dependent inducible nitric oxide synthase from guinea-pig lung."
      Shirato M., Sakamoto T., Uchida Y., Nomura A., Ishii Y., Iijima H., Goto Y., Hasegawa S.
      Biochem. J. 333:795-799(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Hartley.
      Tissue: Lung.

    Entry informationi

    Entry nameiNOS2_CAVPO
    AccessioniPrimary (citable) accession number: O54705
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3