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O54705

- NOS2_CAVPO

UniProt

O54705 - NOS2_CAVPO

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Protein

Nitric oxide synthase, inducible

Gene
NOS2, NOS
Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulationi

Regulated by calcium/calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi199 – 1991Iron (heme axial ligand) By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi622 – 65332FMN By similarityAdd
BLAST
Nucleotide bindingi766 – 77712FAD By similarityAdd
BLAST
Nucleotide bindingi902 – 91211FAD By similarityAdd
BLAST
Nucleotide bindingi977 – 99519NADP By similarityAdd
BLAST
Nucleotide bindingi1075 – 109016NADP By similarityAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro
  5. NADP binding Source: InterPro
  6. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. defense response to bacterium Source: UniProtKB
  2. nitric oxide biosynthetic process Source: InterPro
  3. peptidyl-cysteine S-nitrosylation Source: UniProtKB
  4. superoxide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
Synonyms:NOS
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11491149Nitric oxide synthase, induciblePRO_0000170929Add
BLAST

Expressioni

Tissue specificityi

Expressed in the lung and colon. Not detected in the heart, aorta, liver, kidney, and spleen.

Inductioni

By lipopolysaccharide (LPS); in kidney, spleen, and colon. Expression is reduced in the presence of LPS in lung.

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 By similarity.

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000017277.

Structurei

3D structure databases

ProteinModelPortaliO54705.
SMRiO54705. Positions 82-501, 702-1129.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini538 – 676139Flavodoxin-likeAdd
BLAST
Domaini729 – 969241FAD-binding FR-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni508 – 52821Calmodulin-binding Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Phylogenomic databases

eggNOGiCOG4362.
HOGENOMiHOG000220884.
HOVERGENiHBG000159.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000333. NOS. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54705-1 [UniParc]FASTAAdd to Basket

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MACPWNFLWK LKSSRYDLTE EKDINNNVGK ASHLYSPEIQ DDPKYCSPGK     50
HQNGSSQSLT GTAKKVPESQ SKPHKPSPTC SQHMKIKNWG NGMILQDTLH 100
TKAKTNFTCK PKSCLGSVMN PRSMTRGPRD TPIPPDELLP QAIEFVNQYY 150
DSFKEAKIEE YLARVETVTK EIETTGTYQL TGDELIFATK LAWRNAPRCI 200
GRIQWSNLQV FDARSCHTAQ EMFEHICRHV RYSTNNGNIR SAITVFPQRT 250
DGKHDFRVWN AQLIRYAGYQ MPDGTIQGDP ANLEFTQLCI DLGWKPRYGR 300
FDVLPLILQA DGRDPELFEI PPDLVLEVPM EHPKYEWFQD LGLKWYALPA 350
VANMLLEVGG LEFPACPFNG WYMGTEIGVR DFCDAQRYNI LEEVGRRMGL 400
ETHTLASLWK DRAVTEINVA VLHSFQKQNV TIMDHHSAAE SFMKHMQNEY 450
RARGGCPADW IWLVPPISGS ITPVFHQEML NYILSPFYYY QVEAWKTHVW 500
QDETRRPKRR EIPFRVLAKA TLFASLLMRK MMASRVRATI LFATETGKSE 550
ALAQDLGALF SCAFNPKVLC MDQYQLSSLE EEKLLLVVTS TFGNGDCPGN 600
GETLKKSLFV LKKLTNTFRY AVFGLGSSMY PRFCAFAHDI DIKLSQLGAS 650
QLTPVGEGDE LSGQEDAFCT WAVQTFQAAC AAFDVRGRHH ITIPKRYTSS 700
VTWEPYHYRL VQDSQPLDLN KALSRMHATD VFTMRLKSQK NLQSPKSSRT 750
TLLMELSCDD SRSLAYLPGE HLGVFPCNQP ALVQGILECV VDNPGPHHTV 800
CLEVLDDSGS YWAKDKRLPP CSLSQALTYF LDITTPPTQL QLQKLARLAT 850
EQAERLRLES LSQPSEYNKW KFTNSPTFLE VLEEFPSLRV PAAFLLSQLP 900
ILKPRYYSIS SSLDHTPAEV HLTVAVVTYR TRDGRGPLHH GVCSTWFSGL 950
KPQDPVPCLV RSVNSFQLPK DPSQPCILIG PGTGIAPFRS FWQQRLHNLK 1000
HTGLQGGRMT LLFGCRHPEE DHIYKEEMQE MVQKGVLHEV HTAYSRLPGK 1050
PKAYVQDILR QQLAREVLRV LHEEPGHLYV CGNVLMAQDV ACTLKQLLAA 1100
KLNLNEEQVE DYFFQLKSQK RYHEDIFGAV FPHGVKKDRA ERPPGDDKL 1149
Length:1,149
Mass (Da):130,632
Last modified:June 1, 1998 - v1
Checksum:i66D595A2486DB50E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027180 mRNA. Translation: AAC33177.1.
RefSeqiNP_001166455.1. NM_001172984.1.

Genome annotation databases

GeneIDi100135576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF027180 mRNA. Translation: AAC33177.1 .
RefSeqi NP_001166455.1. NM_001172984.1.

3D structure databases

ProteinModelPortali O54705.
SMRi O54705. Positions 82-501, 702-1129.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000017277.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100135576.

Organism-specific databases

CTDi 4843.

Phylogenomic databases

eggNOGi COG4362.
HOGENOMi HOG000220884.
HOVERGENi HBG000159.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
3.90.340.10. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR004030. NOS_N.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF02898. NO_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000333. NOS. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF56512. SSF56512. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
PS60001. NOS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and characterization of Ca2+-dependent inducible nitric oxide synthase from guinea-pig lung."
    Shirato M., Sakamoto T., Uchida Y., Nomura A., Ishii Y., Iijima H., Goto Y., Hasegawa S.
    Biochem. J. 333:795-799(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hartley.
    Tissue: Lung.

Entry informationi

Entry nameiNOS2_CAVPO
AccessioniPrimary (citable) accession number: O54705
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: September 3, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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