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Protein

Carbohydrate sulfotransferase 10

Gene

Chst10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi127 – 1337PAPSBy similarity
Nucleotide bindingi189 – 1979PAPSBy similarity

GO - Molecular functioni

  • HNK-1 sulfotransferase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-RNO-975578. Reactions specific to the complex N-glycan synthesis pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 10 (EC:2.8.2.-)
Alternative name(s):
HNK-1 sulfotransferase
Short name:
HNK-1ST
Short name:
HNK1ST
Short name:
RaHNK-1ST
Short name:
Sul-T
Gene namesi
Name:Chst10
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi621216. Chst10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini28 – 356329LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356Carbohydrate sulfotransferase 10PRO_0000189660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence analysis
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence analysis
Glycosylationi316 – 3161N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54702.
PRIDEiO54702.

Expressioni

Tissue specificityi

In myogenic progenitors, it is ubiquitously expressed.1 Publication

Gene expression databases

ExpressionAtlasiO54702. baseline and differential.
GenevisibleiO54702. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017714.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050951.
InParanoidiO54702.
KOiK09674.
OMAiPGITRYN.
OrthoDBiEOG7B05D8.
PhylomeDBiO54702.

Family and domain databases

InterProiIPR005331. Sulfotransferase.
[Graphical view]
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54702-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHHQWLLLAA CFWVIFMFMV ASKFITLTFK DPDGYSAKQE FVFLTAMPEA
60 70 80 90 100
EKLRGEKHFS EVMKPTGKML SESHPDQPPV YLERLELIRN ACKEEALRNL
110 120 130 140 150
SHTEVSKFVL DRIFVCDKHK ILFCQTPKVG NTQWKKVLIV LNGAFSSIEE
160 170 180 190 200
IPENVVHDHE KNGLPRLSSF SKIGIQKRLK TYFKFFIVRD PFERLISAFK
210 220 230 240 250
DKFVHNPRFE PWYRHEIAPG IIRKYRKNRT ETRGIQFEDF VRYLGDPNRR
260 270 280 290 300
WLDLQFGDHI IHWVTYVKLC APCEIKYSVI GHHETLEADA PYILKEAGID
310 320 330 340 350
HLVSYPTIPP GITMYNRTKV EQYFLGISKR DIRRLYARFE GDFKLFGYQK

PDFLLN
Length:356
Mass (Da):42,027
Last modified:June 1, 1998 - v1
Checksum:i48DDC990973B5B28
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022729 mRNA. Translation: AAB88123.1.
RefSeqiNP_536322.1. NM_080397.1.
XP_006244794.1. XM_006244732.2.
XP_008765207.1. XM_008766985.1.
XP_008765209.1. XM_008766987.1.
UniGeneiRn.11224.

Genome annotation databases

EnsembliENSRNOT00000017714; ENSRNOP00000017714; ENSRNOG00000012815.
GeneIDi140568.
KEGGirno:140568.
UCSCiRGD:621216. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022729 mRNA. Translation: AAB88123.1.
RefSeqiNP_536322.1. NM_080397.1.
XP_006244794.1. XM_006244732.2.
XP_008765207.1. XM_008766985.1.
XP_008765209.1. XM_008766987.1.
UniGeneiRn.11224.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017714.

Proteomic databases

PaxDbiO54702.
PRIDEiO54702.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017714; ENSRNOP00000017714; ENSRNOG00000012815.
GeneIDi140568.
KEGGirno:140568.
UCSCiRGD:621216. rat.

Organism-specific databases

CTDi9486.
RGDi621216. Chst10.

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050951.
InParanoidiO54702.
KOiK09674.
OMAiPGITRYN.
OrthoDBiEOG7B05D8.
PhylomeDBiO54702.

Enzyme and pathway databases

ReactomeiR-RNO-975578. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

PROiO54702.

Gene expression databases

ExpressionAtlasiO54702. baseline and differential.
GenevisibleiO54702. RN.

Family and domain databases

InterProiIPR005331. Sulfotransferase.
[Graphical view]
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression cloning of a cDNA encoding a sulfotransferase involved in the biosynthesis of the HNK-1 carbohydrate epitope."
    Bakker H., Friedmann I., Oka S., Kawasaki T., Nifant'ev N., Schachner M., Mantei N.
    J. Biol. Chem. 272:29942-29946(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ENZYME ACTIVITY.
    Strain: Sprague-Dawley.
    Tissue: Brain cortex.
  2. "Differential expression of two glucuronyltransferases synthesizing HNK-1 carbohydrate epitope in the sublineages of the rat myogenic progenitors."
    Nagase T., Shimoda Y., Sanai Y., Nakamura S., Harii K., Osumi N.
    Mech. Dev. 98:145-149(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHSTA_RAT
AccessioniPrimary (citable) accession number: O54702
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: June 8, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.