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Protein

Sodium/potassium/calcium exchanger 2

Gene

Slc24a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Critical component of the visual transduction cascade, controlling the calcium concentration of outer segments during light and darkness. Light causes a rapid lowering of cytosolic free calcium in the outer segment of both retinal rod and cone photoreceptors and the light-induced lowering of calcium is caused by extrusion via this protein which plays a key role in the process of light adaptation. Transports 1 Ca2+ and 1 K+ in exchange for 4 Na+.

GO - Molecular functioni

  • cadmium ion binding Source: RGD
  • calcium, potassium:sodium antiporter activity Source: RGD
  • calcium channel activity Source: GO_Central
  • calcium ion binding Source: RGD
  • manganese ion binding Source: RGD
  • nickel cation binding Source: RGD
  • potassium ion binding Source: RGD
  • protein dimerization activity Source: RGD
  • sodium ion binding Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antiport, Calcium transport, Ion transport, Potassium transport, Sensory transduction, Sodium transport, Symport, Transport, Vision

Keywords - Ligandi

Calcium, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-425561. Sodium/Calcium exchangers.

Protein family/group databases

TCDBi2.A.19.4.2. the ca(2+):cation antiporter (caca) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium/calcium exchanger 2
Alternative name(s):
Na(+)/K(+)/Ca(2+)-exchange protein 2
Retinal cone Na-Ca+K exchanger
Solute carrier family 24 member 2
Gene namesi
Name:Slc24a2
Synonyms:Nckx2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi628650. Slc24a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 38CytoplasmicSequence analysisAdd BLAST38
Transmembranei39 – 59HelicalSequence analysisAdd BLAST21
Topological domaini60 – 133ExtracellularSequence analysisAdd BLAST74
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Topological domaini155 – 179CytoplasmicSequence analysisAdd BLAST25
Transmembranei180 – 200HelicalSequence analysisAdd BLAST21
Topological domaini201 – 205ExtracellularSequence analysis5
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21
Topological domaini227 – 244CytoplasmicSequence analysisAdd BLAST18
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266ExtracellularSequence analysis1
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 506CytoplasmicSequence analysisAdd BLAST219
Transmembranei507 – 527HelicalSequence analysisAdd BLAST21
Topological domaini528 – 542ExtracellularSequence analysisAdd BLAST15
Transmembranei543 – 563HelicalSequence analysisAdd BLAST21
Topological domaini564 – 578CytoplasmicSequence analysisAdd BLAST15
Transmembranei579 – 599HelicalSequence analysisAdd BLAST21
Topological domaini600 – 611ExtracellularSequence analysisAdd BLAST12
Transmembranei612 – 632HelicalSequence analysisAdd BLAST21
Topological domaini633 – 639CytoplasmicSequence analysis7
Transmembranei640 – 660HelicalSequence analysisAdd BLAST21
Topological domaini661 – 670ExtracellularSequence analysis10

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intrinsic component of plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000193681 – 670Sodium/potassium/calcium exchanger 2Add BLAST670

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Modified residuei337PhosphoserineCombined sources1
Modified residuei341PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO54701.
PRIDEiO54701.

PTM databases

iPTMnetiO54701.
PhosphoSitePlusiO54701.

Expressioni

Tissue specificityi

Expressed abundantly in all regions of the brain and weakly in the eye, large intestine and adrenal tissue.

Gene expression databases

BgeeiENSRNOG00000008169.
ExpressionAtlasiO54701. baseline and differential.
GenevisibleiO54701. RN.

Interactioni

GO - Molecular functioni

  • protein dimerization activity Source: RGD

Protein-protein interaction databases

BioGridi250001. 1 interactor.
STRINGi10116.ENSRNOP00000010827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati175 – 215Alpha-1Add BLAST41
Repeati550 – 581Alpha-2Add BLAST32

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG054881.
InParanoidiO54701.
KOiK13750.
OMAiSSNTWCH.
OrthoDBiEOG091G0M5C.
PhylomeDBiO54701.
TreeFamiTF318759.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00367. TIGR00367. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O54701-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDLHQSATVR LLQEWCSHES PSGCRRHYNT RKKLKLIRVI GLVMGLVAVS
60 70 80 90 100
TVPFSISAFT ETYSQNNRGE ASDVTGPRAA PGHRQRTLLD LNDKIRDYTP
110 120 130 140 150
QPPASQEDRS ENGTDHAQGD YPKDVFSLEE RRKGAIILHV IGMIYMFIAL
160 170 180 190 200
AIVCDEFFVP SLTVITEKLG ISDDVAGATF MAAGGSAPEL FTSLIGVFIA
210 220 230 240 250
HSNVGIGTIV GSAVFNILFV IGMCALFSRE ILNLTWWPLF RDVSFYIVDL
260 270 280 290 300
IMLIIFFLDN VIMWWESLLL LTAYFAYVVF MKFNVQVERW VKQMINRNKV
310 320 330 340 350
VKVTVSEAQA KASTAGDKEE PTLPNKPRLQ RGGSSASLHN SLMRNSIFQL
360 370 380 390 400
MIHTLDPLAE ELGSYGKLKY YDTMTEEGRF REKASILHKI AKKKCQVDEN
410 420 430 440 450
ERQNGAANHV DYAAEKIELP NSTSTEVEMT PSSEASEPVQ NGNLSHSIEA
460 470 480 490 500
ADAPQATETA EEDDDQPLSL SWPSNTRKQI TFLIVLPIVF PLWITLPDVR
510 520 530 540 550
KPASKKFFPI TFFGSITWIA VFSYLMVWWA HQVGETIGIS EEIMGLTILA
560 570 580 590 600
AGTSIPDLIT SVIVARKGLG DMAVSSSVGS NIFDITVGLP LPWLLYTIIH
610 620 630 640 650
RFKPVTVSSN GLFCAIVLLF IMLIFVILSI ALCKWRMNKI LGFIMFGLYF
660 670
AFLVVSVLLE DKVLECPVSI
Length:670
Mass (Da):74,657
Last modified:June 1, 1998 - v1
Checksum:i4E855A467FC6679E
GO
Isoform 2 (identifier: O54701-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-376: Missing.

Show »
Length:653
Mass (Da):72,647
Checksum:i510AD46CF3884AF5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309Q → R in AAC19404 (PubMed:9461611).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006165360 – 376Missing in isoform 2. 1 PublicationAdd BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021923 mRNA. Translation: AAC19405.1.
AF027506 mRNA. Translation: AAC19404.1.
RefSeqiNP_113931.1. NM_031743.2. [O54701-1]
UniGeneiRn.208755.

Genome annotation databases

EnsembliENSRNOT00000010827; ENSRNOP00000010827; ENSRNOG00000008169. [O54701-1]
ENSRNOT00000083373; ENSRNOP00000074180; ENSRNOG00000008169. [O54701-1]
GeneIDi84550.
KEGGirno:84550.
UCSCiRGD:628650. rat. [O54701-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF021923 mRNA. Translation: AAC19405.1.
AF027506 mRNA. Translation: AAC19404.1.
RefSeqiNP_113931.1. NM_031743.2. [O54701-1]
UniGeneiRn.208755.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250001. 1 interactor.
STRINGi10116.ENSRNOP00000010827.

Protein family/group databases

TCDBi2.A.19.4.2. the ca(2+):cation antiporter (caca) family.

PTM databases

iPTMnetiO54701.
PhosphoSitePlusiO54701.

Proteomic databases

PaxDbiO54701.
PRIDEiO54701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010827; ENSRNOP00000010827; ENSRNOG00000008169. [O54701-1]
ENSRNOT00000083373; ENSRNOP00000074180; ENSRNOG00000008169. [O54701-1]
GeneIDi84550.
KEGGirno:84550.
UCSCiRGD:628650. rat. [O54701-1]

Organism-specific databases

CTDi25769.
RGDi628650. Slc24a2.

Phylogenomic databases

eggNOGiKOG1307. Eukaryota.
ENOG410Y9YY. LUCA.
GeneTreeiENSGT00760000119209.
HOGENOMiHOG000231933.
HOVERGENiHBG054881.
InParanoidiO54701.
KOiK13750.
OMAiSSNTWCH.
OrthoDBiEOG091G0M5C.
PhylomeDBiO54701.
TreeFamiTF318759.

Enzyme and pathway databases

ReactomeiR-RNO-425561. Sodium/Calcium exchangers.

Miscellaneous databases

PROiO54701.

Gene expression databases

BgeeiENSRNOG00000008169.
ExpressionAtlasiO54701. baseline and differential.
GenevisibleiO54701. RN.

Family and domain databases

InterProiIPR004481. K/Na/Ca-exchanger.
IPR004837. NaCa_Exmemb.
[Graphical view]
PANTHERiPTHR10846. PTHR10846. 1 hit.
PfamiPF01699. Na_Ca_ex. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00367. TIGR00367. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCKX2_RAT
AccessioniPrimary (citable) accession number: O54701
Secondary accession number(s): O54706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.