Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Equilibrative nucleoside transporter 2

Gene

Slc29a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is insensitive (EI) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. Specific for nucleosides, but may also transport hypoxanthine. May also play a role in the efflux of inosine and hypoxanthine from muscle cells during the net degradation of purine nucleotides that occurs during strenuous exercise and/or in the reuptake of these purines during the recovery process (By similarity).By similarity

GO - Molecular functioni

  1. nucleoside transmembrane transporter activity Source: RGD

GO - Biological processi

  1. adenine transport Source: RGD
  2. cellular response to insulin stimulus Source: RGD
  3. guanine transport Source: RGD
  4. hypoxanthine transport Source: RGD
  5. lactation Source: RGD
  6. nucleoside transmembrane transport Source: GOC
  7. nucleoside transport Source: RGD
  8. thymine transport Source: RGD
  9. uridine transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_238520. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Protein family/group databases

TCDBi2.A.57.1.4. the equilibrative nucleoside transporter (ent) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 2
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter
Short name:
Equilibrative NBMPR-insensitive nucleoside transporter
Nucleoside transporter, ei-type
Solute carrier family 29 member 2
Gene namesi
Name:Slc29a2
Synonyms:Ent2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi69296. Slc29a2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 2917HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 6839ExtracellularSequence AnalysisAdd
BLAST
Transmembranei69 – 9325HelicalSequence AnalysisAdd
BLAST
Topological domaini94 – 974CytoplasmicSequence Analysis
Transmembranei98 – 11619HelicalSequence AnalysisAdd
BLAST
Topological domaini117 – 1248ExtracellularSequence Analysis
Transmembranei125 – 14319HelicalSequence AnalysisAdd
BLAST
Topological domaini144 – 16017CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei161 – 18525HelicalSequence AnalysisAdd
BLAST
Topological domaini186 – 1927ExtracellularSequence Analysis
Transmembranei193 – 21321HelicalSequence AnalysisAdd
BLAST
Topological domaini214 – 29178CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31120HelicalSequence AnalysisAdd
BLAST
Topological domaini312 – 32312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei324 – 34219HelicalSequence AnalysisAdd
BLAST
Topological domaini343 – 35917CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei360 – 37819HelicalSequence AnalysisAdd
BLAST
Topological domaini379 – 39315ExtracellularSequence AnalysisAdd
BLAST
Transmembranei394 – 41320HelicalSequence AnalysisAdd
BLAST
Topological domaini414 – 43118CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei432 – 45221HelicalSequence AnalysisAdd
BLAST
Topological domaini453 – 4564ExtracellularSequence Analysis

GO - Cellular componenti

  1. integral component of plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Equilibrative nucleoside transporter 2PRO_0000209342Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Modified residuei251 – 2511PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiO54699.

Expressioni

Gene expression databases

GenevestigatoriO54699.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027178.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiO54699.
KOiK15014.
OMAiYELETRA.
OrthoDBiEOG7PP56W.
PhylomeDBiO54699.
TreeFamiTF313950.

Family and domain databases

InterProiIPR030197. ENT2.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF8. PTHR10332:SF8. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequencei

Sequence statusi: Complete.

O54699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHGNAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ GRLAGTNSSA
60 70 80 90 100
ETPSTNHTSP TDTFNFNNWV TLLSQLPLLL FTLLNSFLYQ CIPESVRILG
110 120 130 140 150
SLLAILLLFA LTAALVKVDL SPGLFFSITM ASVWFINSFC AVLQGSLFGQ
160 170 180 190 200
LGTMPSTYST LFLSGQGLAG IFAALAMLTS LASGVDPQTS ALGYFITPCV
210 220 230 240 250
GILLSIICYL SLPHLKFARY YLTKKPQAPV QELETKAELL GADEKNGIPV
260 270 280 290 300
SPQQAGPTLD LDPEKELELG LEEPQKPGKP SVFVVFRKIW LTALCLVLVF
310 320 330 340 350
TVTLSVFPAI TAMVTTSSNS PGKWSQFFNP ICCFLLFNVM DWLGRSLTSY
360 370 380 390 400
FLWPDEDSQL LPLLVCLRFL FVPLFMLCHV PQRARLPIIF WQDAYFITFM
410 420 430 440 450
LLFAISNGYF VSLTMCLAPR QVLPHEREVA GALMTFFLAL GLSCGASLSF

LFKALL
Length:456
Mass (Da):50,265
Last modified:June 1, 1998 - v1
Checksum:iDA97C2C578E1EE9D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015305 mRNA. Translation: AAB88050.1.
RefSeqiNP_113926.1. NM_031738.1.
XP_003749083.1. XM_003749035.3.
UniGeneiRn.37517.

Genome annotation databases

EnsembliENSRNOT00000027178; ENSRNOP00000027178; ENSRNOG00000020025.
ENSRNOT00000071891; ENSRNOP00000065813; ENSRNOG00000050201.
GeneIDi100911721.
65194.
KEGGirno:100911721.
rno:65194.
UCSCiRGD:69296. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015305 mRNA. Translation: AAB88050.1.
RefSeqiNP_113926.1. NM_031738.1.
XP_003749083.1. XM_003749035.3.
UniGeneiRn.37517.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000027178.

Chemistry

ChEMBLiCHEMBL1287614.

Protein family/group databases

TCDBi2.A.57.1.4. the equilibrative nucleoside transporter (ent) family.

Proteomic databases

PRIDEiO54699.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000027178; ENSRNOP00000027178; ENSRNOG00000020025.
ENSRNOT00000071891; ENSRNOP00000065813; ENSRNOG00000050201.
GeneIDi100911721.
65194.
KEGGirno:100911721.
rno:65194.
UCSCiRGD:69296. rat.

Organism-specific databases

CTDi3177.
RGDi69296. Slc29a2.

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiO54699.
KOiK15014.
OMAiYELETRA.
OrthoDBiEOG7PP56W.
PhylomeDBiO54699.
TreeFamiTF313950.

Enzyme and pathway databases

ReactomeiREACT_238520. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

NextBioi614124.
PROiO54699.

Gene expression databases

GenevestigatoriO54699.

Family and domain databases

InterProiIPR030197. ENT2.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF8. PTHR10332:SF8. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues."
    Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A., Young J.D.
    J. Biol. Chem. 272:28423-28430(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Jejunum.

Entry informationi

Entry nameiS29A2_RAT
AccessioniPrimary (citable) accession number: O54699
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: March 4, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.