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O54699 (S29A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Equilibrative nucleoside transporter 2
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter
Short name=Equilibrative NBMPR-insensitive nucleoside transporter
Nucleoside transporter, ei-type
Solute carrier family 29 member 2
Gene names
Name:Slc29a2
Synonyms:Ent2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length456 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is insensitive (EI) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. Specific for nucleosides, but may also transport hypoxanthine. May also play a role in the efflux of inosine and hypoxanthine from muscle cells during the net degradation of purine nucleotides that occurs during strenuous exercise and/or in the reuptake of these purines during the recovery process By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Miscellaneous

Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).

Sequence similarities

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 456456Equilibrative nucleoside transporter 2
PRO_0000209342

Regions

Topological domain1 – 1212Cytoplasmic Potential
Transmembrane13 – 2917Helical; Potential
Topological domain30 – 6839Extracellular Potential
Transmembrane69 – 9325Helical; Potential
Topological domain94 – 974Cytoplasmic Potential
Transmembrane98 – 11619Helical; Potential
Topological domain117 – 1248Extracellular Potential
Transmembrane125 – 14319Helical; Potential
Topological domain144 – 16017Cytoplasmic Potential
Transmembrane161 – 18525Helical; Potential
Topological domain186 – 1927Extracellular Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 29178Cytoplasmic Potential
Transmembrane292 – 31120Helical; Potential
Topological domain312 – 32312Extracellular Potential
Transmembrane324 – 34219Helical; Potential
Topological domain343 – 35917Cytoplasmic Potential
Transmembrane360 – 37819Helical; Potential
Topological domain379 – 39315Extracellular Potential
Transmembrane394 – 41320Helical; Potential
Topological domain414 – 43118Cytoplasmic Potential
Transmembrane432 – 45221Helical; Potential
Topological domain453 – 4564Extracellular Potential

Amino acid modifications

Modified residue2511Phosphoserine By similarity
Glycosylation471N-linked (GlcNAc...) Potential
Glycosylation561N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O54699 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: DA97C2C578E1EE9D

FASTA45650,265
        10         20         30         40         50         60 
MAHGNAPRDS YHLVGISFFI LGLGTLLPWN FFITAIPYFQ GRLAGTNSSA ETPSTNHTSP 

        70         80         90        100        110        120 
TDTFNFNNWV TLLSQLPLLL FTLLNSFLYQ CIPESVRILG SLLAILLLFA LTAALVKVDL 

       130        140        150        160        170        180 
SPGLFFSITM ASVWFINSFC AVLQGSLFGQ LGTMPSTYST LFLSGQGLAG IFAALAMLTS 

       190        200        210        220        230        240 
LASGVDPQTS ALGYFITPCV GILLSIICYL SLPHLKFARY YLTKKPQAPV QELETKAELL 

       250        260        270        280        290        300 
GADEKNGIPV SPQQAGPTLD LDPEKELELG LEEPQKPGKP SVFVVFRKIW LTALCLVLVF 

       310        320        330        340        350        360 
TVTLSVFPAI TAMVTTSSNS PGKWSQFFNP ICCFLLFNVM DWLGRSLTSY FLWPDEDSQL 

       370        380        390        400        410        420 
LPLLVCLRFL FVPLFMLCHV PQRARLPIIF WQDAYFITFM LLFAISNGYF VSLTMCLAPR 

       430        440        450 
QVLPHEREVA GALMTFFLAL GLSCGASLSF LFKALL 

« Hide

References

[1]"Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues."
Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A., Young J.D.
J. Biol. Chem. 272:28423-28430(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Jejunum.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF015305 mRNA. Translation: AAB88050.1.
RefSeqNP_113926.1. NM_031738.1.
XP_003749083.1. XM_003749035.2.
UniGeneRn.37517.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000027178.

Chemistry

ChEMBLCHEMBL1287614.

Protein family/group databases

TCDB2.A.57.1.4. the equilibrative nucleoside transporter (ent) family.

Proteomic databases

PRIDEO54699.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027178; ENSRNOP00000027178; ENSRNOG00000020025.
ENSRNOT00000071891; ENSRNOP00000065813; ENSRNOG00000050201.
GeneID100911721.
65194.
KEGGrno:100911721.
rno:65194.
UCSCRGD:69296. rat.

Organism-specific databases

CTD3177.
RGD69296. Slc29a2.

Phylogenomic databases

eggNOGNOG249415.
GeneTreeENSGT00390000002232.
HOGENOMHOG000007684.
HOVERGENHBG074626.
InParanoidO54699.
KOK15014.
OMAINSFCAI.
OrthoDBEOG7PP56W.
PhylomeDBO54699.
TreeFamTF313950.

Gene expression databases

GenevestigatorO54699.

Family and domain databases

InterProIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERPTHR10332. PTHR10332. 1 hit.
PfamPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFPIRSF016379. ENT. 1 hit.
PRINTSPR01130. DERENTRNSPRT.
SUPFAMSSF103473. SSF103473. 2 hits.
TIGRFAMsTIGR00939. 2a57. 1 hit.
ProtoNetSearch...

Other

NextBio614124.
PROO54699.

Entry information

Entry nameS29A2_RAT
AccessionPrimary (citable) accession number: O54699
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families