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Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).1 Publication

GO - Molecular functioni

  1. nucleoside transmembrane transporter activity Source: RGD

GO - Biological processi

  1. cellular response to glucose stimulus Source: RGD
  2. cellular response to hypoxia Source: RGD
  3. lactation Source: RGD
  4. nucleoside transmembrane transport Source: GOC
  5. nucleoside transport Source: RGD
  6. regulation of excitatory postsynaptic membrane potential Source: RGD
  7. sleep Source: RGD
  8. uridine transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_238520. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Protein family/group databases

TCDBi2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi61899. Slc29a1.

Subcellular locationi

Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity. Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 2917HelicalSequence AnalysisAdd
BLAST
Topological domaini30 – 8253ExtracellularSequence AnalysisAdd
BLAST
Transmembranei83 – 10725HelicalSequence AnalysisAdd
BLAST
Topological domaini108 – 1114CytoplasmicSequence Analysis
Transmembranei112 – 13019HelicalSequence AnalysisAdd
BLAST
Topological domaini131 – 1388ExtracellularSequence Analysis
Transmembranei139 – 15719HelicalSequence AnalysisAdd
BLAST
Topological domaini158 – 17417CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei175 – 19925HelicalSequence AnalysisAdd
BLAST
Topological domaini200 – 2067ExtracellularSequence Analysis
Transmembranei207 – 22721HelicalSequence AnalysisAdd
BLAST
Topological domaini228 – 29164CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei292 – 31120HelicalSequence AnalysisAdd
BLAST
Topological domaini312 – 32312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei324 – 34320HelicalSequence AnalysisAdd
BLAST
Topological domaini344 – 36017CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei361 – 37919HelicalSequence AnalysisAdd
BLAST
Topological domaini380 – 39415ExtracellularSequence AnalysisAdd
BLAST
Transmembranei395 – 41420HelicalSequence AnalysisAdd
BLAST
Topological domaini415 – 43218CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei433 – 45321HelicalSequence AnalysisAdd
BLAST
Topological domaini454 – 4574ExtracellularSequence Analysis

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basolateral plasma membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 457456Equilibrative nucleoside transporter 1PRO_0000209339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54698.
PRIDEiO54698.

Expressioni

Tissue specificityi

Expressed in jejunum, liver and lung.

Gene expression databases

GenevestigatoriO54698.

Interactioni

Subunit structurei

Identified in a complex with STOM.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026831.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiO54698.
KOiK15014.
OMAiSTWERYF.
OrthoDBiEOG7PP56W.
PhylomeDBiO54698.

Family and domain databases

InterProiIPR030195. ENT1.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF9. PTHR10332:SF9. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54698-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFITATQYFT SRLNTSQNIS
60 70 80 90 100
LVTNQSCEST EALADPSVSL PARSSLSAIF NNVMTLCAML PLLIFTCLNS
110 120 130 140 150
FLHQKVSQSL RILGSLLAIL LVFLVTATLV KVQMDALSFF IITMIKIVLI
160 170 180 190 200
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAVASGSK
210 220 230 240 250
LSESAFGYFI TACAVVILAI LCYLALPWME FYRHYLQLNL AGPAEQETKL
260 270 280 290 300
DLISEGEEPR GGREESGVPG PNSLPANRNQ SIKAILKSIW VLALSVCFIF
310 320 330 340 350
TVTIGLFPAV TAEVESSIAG TSPWKNCYFI PVACFLNFNV FDWLGRSLTA
360 370 380 390 400
ICMWPGQDSR WLPVLVACRV VFIPLLMLCN VKQHHYLPSL FKHDVWFITF
410 420 430 440 450
MAAFAFSNGY LASLCMCFGP KKVKPAEAET AGNIMSFFLC LGLALGAVLS

FLLRALV
Length:457
Mass (Da):50,017
Last modified:January 23, 2007 - v3
Checksum:iA9DDF4F0D7F8D70A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015304 mRNA. Translation: AAB88049.1.
BC078789 mRNA. Translation: AAH78789.1.
RefSeqiNP_113872.1. NM_031684.2.
XP_006244622.1. XM_006244560.1.
XP_006244623.1. XM_006244561.2.
XP_006244624.1. XM_006244562.2.
XP_006244625.1. XM_006244563.2.
XP_008765101.1. XM_008766879.1.
UniGeneiRn.5814.

Genome annotation databases

EnsembliENSRNOT00000026831; ENSRNOP00000026831; ENSRNOG00000019752.
GeneIDi63997.
KEGGirno:63997.
UCSCiRGD:61899. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF015304 mRNA. Translation: AAB88049.1.
BC078789 mRNA. Translation: AAH78789.1.
RefSeqiNP_113872.1. NM_031684.2.
XP_006244622.1. XM_006244560.1.
XP_006244623.1. XM_006244561.2.
XP_006244624.1. XM_006244562.2.
XP_006244625.1. XM_006244563.2.
XP_008765101.1. XM_008766879.1.
UniGeneiRn.5814.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026831.

Chemistry

BindingDBiO54698.
ChEMBLiCHEMBL4604.

Protein family/group databases

TCDBi2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

Proteomic databases

PaxDbiO54698.
PRIDEiO54698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026831; ENSRNOP00000026831; ENSRNOG00000019752.
GeneIDi63997.
KEGGirno:63997.
UCSCiRGD:61899. rat.

Organism-specific databases

CTDi2030.
RGDi61899. Slc29a1.

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiO54698.
KOiK15014.
OMAiSTWERYF.
OrthoDBiEOG7PP56W.
PhylomeDBiO54698.

Enzyme and pathway databases

ReactomeiREACT_238520. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

NextBioi612562.
PROiO54698.

Gene expression databases

GenevestigatoriO54698.

Family and domain databases

InterProiIPR030195. ENT1.
IPR002259. Eqnu_transpt.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PTHR10332:SF9. PTHR10332:SF9. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues."
    Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A., Young J.D.
    J. Biol. Chem. 272:28423-28430(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiS29A1_RAT
AccessioniPrimary (citable) accession number: O54698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.