Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O54698

- S29A1_RAT

UniProt

O54698 - S29A1_RAT

Protein

Equilibrative nucleoside transporter 1

Gene

Slc29a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).1 Publication

    GO - Molecular functioni

    1. nucleoside transmembrane transporter activity Source: RGD

    GO - Biological processi

    1. cellular response to glucose stimulus Source: RGD
    2. cellular response to hypoxia Source: RGD
    3. lactation Source: RGD
    4. nucleoside transmembrane transport Source: GOC
    5. nucleoside transport Source: RGD
    6. regulation of excitatory postsynaptic membrane potential Source: RGD
    7. sleep Source: RGD
    8. uridine transport Source: RGD

    Keywords - Biological processi

    Transport

    Protein family/group databases

    TCDBi2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Equilibrative nucleoside transporter 1
    Alternative name(s):
    Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
    Short name:
    Equilibrative NBMPR-sensitive nucleoside transporter
    Nucleoside transporter, es-type
    Solute carrier family 29 member 1
    Gene namesi
    Name:Slc29a1
    Synonyms:Ent1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi61899. Slc29a1.

    Subcellular locationi

    Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity. Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Predominantly localized in the basolateral membrane in polarized MDCK cells.By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. basolateral plasma membrane Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 457456Equilibrative nucleoside transporter 1PRO_0000209339Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi48 – 481N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO54698.
    PRIDEiO54698.

    Expressioni

    Tissue specificityi

    Expressed in jejunum, liver and lung.

    Gene expression databases

    GenevestigatoriO54698.

    Interactioni

    Subunit structurei

    Identified in a complex with STOM.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000026831.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 1211CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini30 – 8253ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini108 – 1114CytoplasmicSequence Analysis
    Topological domaini131 – 1388ExtracellularSequence Analysis
    Topological domaini158 – 17417CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini200 – 2067ExtracellularSequence Analysis
    Topological domaini228 – 29164CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini312 – 32312ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini344 – 36017CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini380 – 39415ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini415 – 43218CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini454 – 4574ExtracellularSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 2917HelicalSequence AnalysisAdd
    BLAST
    Transmembranei83 – 10725HelicalSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei139 – 15719HelicalSequence AnalysisAdd
    BLAST
    Transmembranei175 – 19925HelicalSequence AnalysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 31120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei324 – 34320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei361 – 37919HelicalSequence AnalysisAdd
    BLAST
    Transmembranei395 – 41420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei433 – 45321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249415.
    GeneTreeiENSGT00390000002232.
    HOGENOMiHOG000007684.
    HOVERGENiHBG074626.
    InParanoidiO54698.
    KOiK15014.
    OMAiSTWERYF.
    OrthoDBiEOG7PP56W.
    PhylomeDBiO54698.

    Family and domain databases

    InterProiIPR002259. Eqnu_transpt.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view]
    PANTHERiPTHR10332. PTHR10332. 1 hit.
    PfamiPF01733. Nucleoside_tran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016379. ENT. 1 hit.
    PRINTSiPR01130. DERENTRNSPRT.
    SUPFAMiSSF103473. SSF103473. 3 hits.
    TIGRFAMsiTIGR00939. 2a57. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O54698-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFITATQYFT SRLNTSQNIS    50
    LVTNQSCEST EALADPSVSL PARSSLSAIF NNVMTLCAML PLLIFTCLNS 100
    FLHQKVSQSL RILGSLLAIL LVFLVTATLV KVQMDALSFF IITMIKIVLI 150
    NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAVASGSK 200
    LSESAFGYFI TACAVVILAI LCYLALPWME FYRHYLQLNL AGPAEQETKL 250
    DLISEGEEPR GGREESGVPG PNSLPANRNQ SIKAILKSIW VLALSVCFIF 300
    TVTIGLFPAV TAEVESSIAG TSPWKNCYFI PVACFLNFNV FDWLGRSLTA 350
    ICMWPGQDSR WLPVLVACRV VFIPLLMLCN VKQHHYLPSL FKHDVWFITF 400
    MAAFAFSNGY LASLCMCFGP KKVKPAEAET AGNIMSFFLC LGLALGAVLS 450
    FLLRALV 457
    Length:457
    Mass (Da):50,017
    Last modified:January 23, 2007 - v3
    Checksum:iA9DDF4F0D7F8D70A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF015304 mRNA. Translation: AAB88049.1.
    BC078789 mRNA. Translation: AAH78789.1.
    RefSeqiNP_113872.1. NM_031684.2.
    XP_006244622.1. XM_006244560.1.
    XP_006244623.1. XM_006244561.1.
    XP_006244624.1. XM_006244562.1.
    XP_006244625.1. XM_006244563.1.
    XP_006244626.1. XM_006244564.1.
    UniGeneiRn.5814.

    Genome annotation databases

    EnsembliENSRNOT00000026831; ENSRNOP00000026831; ENSRNOG00000019752.
    GeneIDi63997.
    KEGGirno:63997.
    UCSCiRGD:61899. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF015304 mRNA. Translation: AAB88049.1 .
    BC078789 mRNA. Translation: AAH78789.1 .
    RefSeqi NP_113872.1. NM_031684.2.
    XP_006244622.1. XM_006244560.1.
    XP_006244623.1. XM_006244561.1.
    XP_006244624.1. XM_006244562.1.
    XP_006244625.1. XM_006244563.1.
    XP_006244626.1. XM_006244564.1.
    UniGenei Rn.5814.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000026831.

    Chemistry

    BindingDBi O54698.
    ChEMBLi CHEMBL4604.

    Protein family/group databases

    TCDBi 2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

    Proteomic databases

    PaxDbi O54698.
    PRIDEi O54698.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000026831 ; ENSRNOP00000026831 ; ENSRNOG00000019752 .
    GeneIDi 63997.
    KEGGi rno:63997.
    UCSCi RGD:61899. rat.

    Organism-specific databases

    CTDi 2030.
    RGDi 61899. Slc29a1.

    Phylogenomic databases

    eggNOGi NOG249415.
    GeneTreei ENSGT00390000002232.
    HOGENOMi HOG000007684.
    HOVERGENi HBG074626.
    InParanoidi O54698.
    KOi K15014.
    OMAi STWERYF.
    OrthoDBi EOG7PP56W.
    PhylomeDBi O54698.

    Miscellaneous databases

    NextBioi 612562.
    PROi O54698.

    Gene expression databases

    Genevestigatori O54698.

    Family and domain databases

    InterProi IPR002259. Eqnu_transpt.
    IPR016196. MFS_dom_general_subst_transpt.
    [Graphical view ]
    PANTHERi PTHR10332. PTHR10332. 1 hit.
    Pfami PF01733. Nucleoside_tran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016379. ENT. 1 hit.
    PRINTSi PR01130. DERENTRNSPRT.
    SUPFAMi SSF103473. SSF103473. 3 hits.
    TIGRFAMsi TIGR00939. 2a57. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues."
      Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A., Young J.D.
      J. Biol. Chem. 272:28423-28430(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: Sprague-Dawley.
      Tissue: Jejunum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiS29A1_RAT
    AccessioniPrimary (citable) accession number: O54698
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 102 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3