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O54698

- S29A1_RAT

UniProt

O54698 - S29A1_RAT

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Protein
Equilibrative nucleoside transporter 1
Gene
Slc29a1, Ent1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs).1 Publication

GO - Molecular functioni

  1. nucleoside transmembrane transporter activity Source: RGD

GO - Biological processi

  1. cellular response to glucose stimulus Source: RGD
  2. cellular response to hypoxia Source: RGD
  3. lactation Source: RGD
  4. nucleoside transmembrane transport Source: GOC
  5. nucleoside transport Source: RGD
  6. regulation of excitatory postsynaptic membrane potential Source: RGD
  7. sleep Source: RGD
  8. uridine transport Source: RGD
Complete GO annotation...

Keywords - Biological processi

Transport

Protein family/group databases

TCDBi2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name:
Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene namesi
Name:Slc29a1
Synonyms:Ent1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 9

Organism-specific databases

RGDi61899. Slc29a1.

Subcellular locationi

Basolateral cell membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein
Note: Predominantly localized in the basolateral membrane in polarized MDCK cells By similarity.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei13 – 2917Helical; Reviewed prediction
Add
BLAST
Topological domaini30 – 8253Extracellular Reviewed prediction
Add
BLAST
Transmembranei83 – 10725Helical; Reviewed prediction
Add
BLAST
Topological domaini108 – 1114Cytoplasmic Reviewed prediction
Transmembranei112 – 13019Helical; Reviewed prediction
Add
BLAST
Topological domaini131 – 1388Extracellular Reviewed prediction
Transmembranei139 – 15719Helical; Reviewed prediction
Add
BLAST
Topological domaini158 – 17417Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei175 – 19925Helical; Reviewed prediction
Add
BLAST
Topological domaini200 – 2067Extracellular Reviewed prediction
Transmembranei207 – 22721Helical; Reviewed prediction
Add
BLAST
Topological domaini228 – 29164Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei292 – 31120Helical; Reviewed prediction
Add
BLAST
Topological domaini312 – 32312Extracellular Reviewed prediction
Add
BLAST
Transmembranei324 – 34320Helical; Reviewed prediction
Add
BLAST
Topological domaini344 – 36017Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei361 – 37919Helical; Reviewed prediction
Add
BLAST
Topological domaini380 – 39415Extracellular Reviewed prediction
Add
BLAST
Transmembranei395 – 41420Helical; Reviewed prediction
Add
BLAST
Topological domaini415 – 43218Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei433 – 45321Helical; Reviewed prediction
Add
BLAST
Topological domaini454 – 4574Extracellular Reviewed prediction

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. basolateral plasma membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 457456Equilibrative nucleoside transporter 1
PRO_0000209339Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...) Reviewed prediction
Glycosylationi48 – 481N-linked (GlcNAc...) Reviewed prediction
Glycosylationi54 – 541N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54698.
PRIDEiO54698.

Expressioni

Tissue specificityi

Expressed in jejunum, liver and lung.

Gene expression databases

GenevestigatoriO54698.

Interactioni

Subunit structurei

Identified in a complex with STOM By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026831.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249415.
GeneTreeiENSGT00390000002232.
HOGENOMiHOG000007684.
HOVERGENiHBG074626.
InParanoidiO54698.
KOiK15014.
OMAiSTWERYF.
OrthoDBiEOG7PP56W.
PhylomeDBiO54698.

Family and domain databases

InterProiIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERiPTHR10332. PTHR10332. 1 hit.
PfamiPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFiPIRSF016379. ENT. 1 hit.
PRINTSiPR01130. DERENTRNSPRT.
SUPFAMiSSF103473. SSF103473. 3 hits.
TIGRFAMsiTIGR00939. 2a57. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54698-1 [UniParc]FASTAAdd to Basket

« Hide

MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFITATQYFT SRLNTSQNIS    50
LVTNQSCEST EALADPSVSL PARSSLSAIF NNVMTLCAML PLLIFTCLNS 100
FLHQKVSQSL RILGSLLAIL LVFLVTATLV KVQMDALSFF IITMIKIVLI 150
NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ GLAGFFTSVA MICAVASGSK 200
LSESAFGYFI TACAVVILAI LCYLALPWME FYRHYLQLNL AGPAEQETKL 250
DLISEGEEPR GGREESGVPG PNSLPANRNQ SIKAILKSIW VLALSVCFIF 300
TVTIGLFPAV TAEVESSIAG TSPWKNCYFI PVACFLNFNV FDWLGRSLTA 350
ICMWPGQDSR WLPVLVACRV VFIPLLMLCN VKQHHYLPSL FKHDVWFITF 400
MAAFAFSNGY LASLCMCFGP KKVKPAEAET AGNIMSFFLC LGLALGAVLS 450
FLLRALV 457
Length:457
Mass (Da):50,017
Last modified:January 23, 2007 - v3
Checksum:iA9DDF4F0D7F8D70A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF015304 mRNA. Translation: AAB88049.1.
BC078789 mRNA. Translation: AAH78789.1.
RefSeqiNP_113872.1. NM_031684.2.
XP_006244622.1. XM_006244560.1.
XP_006244623.1. XM_006244561.1.
XP_006244624.1. XM_006244562.1.
XP_006244625.1. XM_006244563.1.
XP_006244626.1. XM_006244564.1.
UniGeneiRn.5814.

Genome annotation databases

EnsembliENSRNOT00000026831; ENSRNOP00000026831; ENSRNOG00000019752.
GeneIDi63997.
KEGGirno:63997.
UCSCiRGD:61899. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF015304 mRNA. Translation: AAB88049.1 .
BC078789 mRNA. Translation: AAH78789.1 .
RefSeqi NP_113872.1. NM_031684.2.
XP_006244622.1. XM_006244560.1.
XP_006244623.1. XM_006244561.1.
XP_006244624.1. XM_006244562.1.
XP_006244625.1. XM_006244563.1.
XP_006244626.1. XM_006244564.1.
UniGenei Rn.5814.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000026831.

Chemistry

BindingDBi O54698.
ChEMBLi CHEMBL4604.

Protein family/group databases

TCDBi 2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

Proteomic databases

PaxDbi O54698.
PRIDEi O54698.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000026831 ; ENSRNOP00000026831 ; ENSRNOG00000019752 .
GeneIDi 63997.
KEGGi rno:63997.
UCSCi RGD:61899. rat.

Organism-specific databases

CTDi 2030.
RGDi 61899. Slc29a1.

Phylogenomic databases

eggNOGi NOG249415.
GeneTreei ENSGT00390000002232.
HOGENOMi HOG000007684.
HOVERGENi HBG074626.
InParanoidi O54698.
KOi K15014.
OMAi STWERYF.
OrthoDBi EOG7PP56W.
PhylomeDBi O54698.

Miscellaneous databases

NextBioi 612562.
PROi O54698.

Gene expression databases

Genevestigatori O54698.

Family and domain databases

InterProi IPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view ]
PANTHERi PTHR10332. PTHR10332. 1 hit.
Pfami PF01733. Nucleoside_tran. 1 hit.
[Graphical view ]
PIRSFi PIRSF016379. ENT. 1 hit.
PRINTSi PR01130. DERENTRNSPRT.
SUPFAMi SSF103473. SSF103473. 3 hits.
TIGRFAMsi TIGR00939. 2a57. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues."
    Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A., Young J.D.
    J. Biol. Chem. 272:28423-28430(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: Sprague-Dawley.
    Tissue: Jejunum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiS29A1_RAT
AccessioniPrimary (citable) accession number: O54698
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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