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O54698 (S29A1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Equilibrative nucleoside transporter 1
Alternative name(s):
Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter
Short name=Equilibrative NBMPR-sensitive nucleoside transporter
Nucleoside transporter, es-type
Solute carrier family 29 member 1
Gene names
Name:Slc29a1
Synonyms:Ent1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Resistant to dipyridamole and dilazep inhibition (anticancer chemotherapeutics drugs). Ref.1

Subunit structure

Identified in a complex with STOM By similarity.

Subcellular location

Basolateral cell membrane; Multi-pass membrane protein By similarity. Apical cell membrane; Multi-pass membrane protein By similarity. Cell membrane; Multi-pass membrane protein. Note: Predominantly localized in the basolateral membrane in polarized MDCK cells By similarity. Ref.1

Tissue specificity

Expressed in jejunum, liver and lung.

Post-translational modification

Glycosylated.

Sequence similarities

Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 457456Equilibrative nucleoside transporter 1
PRO_0000209339

Regions

Topological domain2 – 1211Cytoplasmic Potential
Transmembrane13 – 2917Helical; Potential
Topological domain30 – 8253Extracellular Potential
Transmembrane83 – 10725Helical; Potential
Topological domain108 – 1114Cytoplasmic Potential
Transmembrane112 – 13019Helical; Potential
Topological domain131 – 1388Extracellular Potential
Transmembrane139 – 15719Helical; Potential
Topological domain158 – 17417Cytoplasmic Potential
Transmembrane175 – 19925Helical; Potential
Topological domain200 – 2067Extracellular Potential
Transmembrane207 – 22721Helical; Potential
Topological domain228 – 29164Cytoplasmic Potential
Transmembrane292 – 31120Helical; Potential
Topological domain312 – 32312Extracellular Potential
Transmembrane324 – 34320Helical; Potential
Topological domain344 – 36017Cytoplasmic Potential
Transmembrane361 – 37919Helical; Potential
Topological domain380 – 39415Extracellular Potential
Transmembrane395 – 41420Helical; Potential
Topological domain415 – 43218Cytoplasmic Potential
Transmembrane433 – 45321Helical; Potential
Topological domain454 – 4574Extracellular Potential

Amino acid modifications

Glycosylation441N-linked (GlcNAc...) Potential
Glycosylation481N-linked (GlcNAc...) Potential
Glycosylation541N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O54698 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A9DDF4F0D7F8D70A

FASTA45750,017
        10         20         30         40         50         60 
MTTSHQPQDR YKAVWLIFFV LGLGTLLPWN FFITATQYFT SRLNTSQNIS LVTNQSCEST 

        70         80         90        100        110        120 
EALADPSVSL PARSSLSAIF NNVMTLCAML PLLIFTCLNS FLHQKVSQSL RILGSLLAIL 

       130        140        150        160        170        180 
LVFLVTATLV KVQMDALSFF IITMIKIVLI NSFGAILQAS LFGLAGVLPA NYTAPIMSGQ 

       190        200        210        220        230        240 
GLAGFFTSVA MICAVASGSK LSESAFGYFI TACAVVILAI LCYLALPWME FYRHYLQLNL 

       250        260        270        280        290        300 
AGPAEQETKL DLISEGEEPR GGREESGVPG PNSLPANRNQ SIKAILKSIW VLALSVCFIF 

       310        320        330        340        350        360 
TVTIGLFPAV TAEVESSIAG TSPWKNCYFI PVACFLNFNV FDWLGRSLTA ICMWPGQDSR 

       370        380        390        400        410        420 
WLPVLVACRV VFIPLLMLCN VKQHHYLPSL FKHDVWFITF MAAFAFSNGY LASLCMCFGP 

       430        440        450 
KKVKPAEAET AGNIMSFFLC LGLALGAVLS FLLRALV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues."
Yao S.Y.M., Ng A.M.L., Muzyka W.R., Griffiths M., Cass C.E., Baldwin S.A., Young J.D.
J. Biol. Chem. 272:28423-28430(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION.
Strain: Sprague-Dawley.
Tissue: Jejunum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF015304 mRNA. Translation: AAB88049.1.
BC078789 mRNA. Translation: AAH78789.1.
RefSeqNP_113872.1. NM_031684.2.
XP_006244622.1. XM_006244560.1.
XP_006244623.1. XM_006244561.1.
XP_006244624.1. XM_006244562.1.
XP_006244625.1. XM_006244563.1.
XP_006244626.1. XM_006244564.1.
UniGeneRn.5814.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000026831.

Chemistry

BindingDBO54698.
ChEMBLCHEMBL4604.

Protein family/group databases

TCDB2.A.57.1.3. the equilibrative nucleoside transporter (ent) family.

Proteomic databases

PaxDbO54698.
PRIDEO54698.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000026831; ENSRNOP00000026831; ENSRNOG00000019752.
GeneID63997.
KEGGrno:63997.
UCSCRGD:61899. rat.

Organism-specific databases

CTD2030.
RGD61899. Slc29a1.

Phylogenomic databases

eggNOGNOG249415.
GeneTreeENSGT00390000002232.
HOGENOMHOG000007684.
HOVERGENHBG074626.
InParanoidO54698.
KOK15014.
OMASTWERYF.
OrthoDBEOG7PP56W.
PhylomeDBO54698.

Gene expression databases

GenevestigatorO54698.

Family and domain databases

InterProIPR002259. Eqnu_transpt.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PANTHERPTHR10332. PTHR10332. 1 hit.
PfamPF01733. Nucleoside_tran. 1 hit.
[Graphical view]
PIRSFPIRSF016379. ENT. 1 hit.
PRINTSPR01130. DERENTRNSPRT.
SUPFAMSSF103473. SSF103473. 3 hits.
TIGRFAMsTIGR00939. 2a57. 1 hit.
ProtoNetSearch...

Other

NextBio612562.
PROO54698.

Entry information

Entry nameS29A1_RAT
AccessionPrimary (citable) accession number: O54698
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 101 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families