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Protein

N-acetylated-alpha-linked acidic dipeptidase-like protein

Gene

Naaladl1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has no NAAG hydrolyzing activity (By similarity). Exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.By similarity

Catalytic activityi

Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi373 – 3731Zinc 1Sequence analysis
Metal bindingi383 – 3831Zinc 1By similarity
Metal bindingi383 – 3831Zinc 2By similarity
Active sitei421 – 4211Nucleophile; for NAALADase activityBy similarity
Metal bindingi422 – 4221Zinc 2By similarity
Metal bindingi450 – 4501Zinc 1By similarity
Metal bindingi550 – 5501Zinc 2By similarity
Active sitei622 – 6221Charge relay systemSequence analysis
Active sitei662 – 6621Charge relay systemSequence analysis
Active sitei685 – 6851Charge relay systemSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Dipeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM28.011.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylated-alpha-linked acidic dipeptidase-like protein (EC:3.4.17.21)
Short name:
NAALADase L
Alternative name(s):
100 kDa ileum brush border membrane protein
Short name:
I100
Ileal dipeptidylpeptidase
Gene namesi
Name:Naaladl1
Synonyms:Naaladl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620987. Naaladl1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence analysis
Transmembranei7 – 2822Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini29 – 745717ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745N-acetylated-alpha-linked acidic dipeptidase-like proteinPRO_0000174125Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence analysis
Glycosylationi141 – 1411N-linked (GlcNAc...)Sequence analysis
Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence analysis
Glycosylationi279 – 2791N-linked (GlcNAc...)Sequence analysis
Glycosylationi304 – 3041N-linked (GlcNAc...)Sequence analysis
Glycosylationi350 – 3501N-linked (GlcNAc...)Sequence analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence analysis
Glycosylationi497 – 4971N-linked (GlcNAc...)Sequence analysis
Glycosylationi593 – 5931N-linked (GlcNAc...)Sequence analysis
Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO54697.
PRIDEiO54697.

PTM databases

iPTMnetiO54697.
PhosphoSiteiO54697.

Expressioni

Tissue specificityi

Mainly expressed in the distal small intestine.

Gene expression databases

BgeeiENSRNOG00000021000.
GenevisibleiO54697. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051719.

Structurei

3D structure databases

ProteinModelPortaliO54697.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni268 – 584317NAALADaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi635 – 6395Poly-Ala

Sequence similaritiesi

Belongs to the peptidase M28 family. M28B subfamily.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
GeneTreeiENSGT00550000074421.
HOGENOMiHOG000211921.
HOVERGENiHBG051639.
InParanoidiO54697.
KOiK01301.
OMAiTSMDLAY.
OrthoDBiEOG091G02ZM.
PhylomeDBiO54697.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR003137. PA_domain.
IPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.

Sequencei

Sequence statusi: Complete.

O54697-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWAKILGVG IGAAALLGLG IILGHFAIPK ATEPLASSVS DSQDLDLAIL
60 70 80 90 100
DSVMGQLDAS RIRENLRELS KEPHVATSAR DEALVQLLLG RWKDSASGLD
110 120 130 140 150
TAKTYEYTVL LSFPSTEQPN SVEVVGPNGT VFHSFQPFEK NLTGEQAEPN
160 170 180 190 200
VLQPYAAYAP PGTPKGPLVY ANRGSEDDFK KLEAEGINLK GTIALTRYGS
210 220 230 240 250
VGRGAKAINA ARHGVVGVLV YTDPGDINDG KSLPNETFPN SWGLPPSGVE
260 270 280 290 300
RGSYYEYFGD PLTPYLPAHP VSFRLDPHNI SGFPPIPTQP IGFEDAKNLL
310 320 330 340 350
CNLNGTSAPD SWQGALGCEY KLGPGFEPNG NFPAGSEVKV SVYNRLELRN
360 370 380 390 400
SSNVLGIIQG AVEPDRYVIY GNHRDSWVHG AVDPSSGTAV LLEISRVLGT
410 420 430 440 450
LLKKGTWRPR RSIIFASWGA EEFGLIGSTE FTEEFLSKLQ ERTVTYINVD
460 470 480 490 500
ISVFSNATLR AQGTPPVQSV IFSATKEISA PGSSGLSIYD NWIRYTNRSS
510 520 530 540 550
PVYGLVPSMG TLGAGSDYAS FIHFLGITSM DLAYTYDRSK TSARIYPTYH
560 570 580 590 600
TAFDTFDYVE KFLDPGFSSH QAVARTAGSV LLRLSDSLFL PLNVSDYSET
610 620 630 640 650
LQSFLQAAQE NLGALLESHN ISLGPLVTAV EKFKAAAAAL NQHILTLQKS
660 670 680 690 700
SPDPLQVRMV NDQLMLLERA FLNPRAFPEE RYYSHVLWAP NTASVATFPG
710 720 730 740
LANAYARAQE INSGAEAWAE VERQLSIAVM ALEGAAATLQ PVTDL
Length:745
Mass (Da):80,641
Last modified:June 1, 1998 - v1
Checksum:iA59C2EFD23BE36B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009921 mRNA. Translation: AAB87644.1.
RefSeqiNP_113947.1. NM_031759.1.
UniGeneiRn.10856.

Genome annotation databases

EnsembliENSRNOT00000054835; ENSRNOP00000051719; ENSRNOG00000021000.
GeneIDi83568.
KEGGirno:83568.
UCSCiRGD:620987. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009921 mRNA. Translation: AAB87644.1.
RefSeqiNP_113947.1. NM_031759.1.
UniGeneiRn.10856.

3D structure databases

ProteinModelPortaliO54697.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051719.

Protein family/group databases

MEROPSiM28.011.

PTM databases

iPTMnetiO54697.
PhosphoSiteiO54697.

Proteomic databases

PaxDbiO54697.
PRIDEiO54697.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000054835; ENSRNOP00000051719; ENSRNOG00000021000.
GeneIDi83568.
KEGGirno:83568.
UCSCiRGD:620987. rat.

Organism-specific databases

CTDi10004.
RGDi620987. Naaladl1.

Phylogenomic databases

eggNOGiKOG2195. Eukaryota.
COG2234. LUCA.
GeneTreeiENSGT00550000074421.
HOGENOMiHOG000211921.
HOVERGENiHBG051639.
InParanoidiO54697.
KOiK01301.
OMAiTSMDLAY.
OrthoDBiEOG091G02ZM.
PhylomeDBiO54697.

Miscellaneous databases

PROiO54697.

Gene expression databases

BgeeiENSRNOG00000021000.
GenevisibleiO54697. RN.

Family and domain databases

Gene3Di1.20.930.40. 1 hit.
InterProiIPR003137. PA_domain.
IPR007484. Peptidase_M28.
IPR007365. TFR-like_dimer_dom.
[Graphical view]
PfamiPF02225. PA. 1 hit.
PF04389. Peptidase_M28. 1 hit.
PF04253. TFR_dimer. 1 hit.
[Graphical view]
SUPFAMiSSF47672. SSF47672. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNALDL_RAT
AccessioniPrimary (citable) accession number: O54697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.