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O54695

- SPTC1_CRIGR

UniProt

O54695 - SPTC1_CRIGR

Protein

Serine palmitoyltransferase 1

Gene

SPTLC1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs By similarity.By similarity

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. sphingomyelin biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 1 (EC:2.3.1.50)
    Alternative name(s):
    Long chain base biosynthesis protein 1
    Short name:
    LCB 1
    Serine-palmitoyl-CoA transferase 1
    Short name:
    SPT 1
    Short name:
    SPT1
    Gene namesi
    Name:SPTLC1
    Synonyms:LCB1
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. serine C-palmitoyltransferase complex Source: MGI
    3. SPOTS complex Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Serine palmitoyltransferase 1PRO_0000163852Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei164 – 1641Phosphotyrosine; by ABLBy similarity

    Post-translational modificationi

    Phosphorylation at Tyr-164 inhibits activity and promotes cell survival.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiO54695.

    Interactioni

    Subunit structurei

    Heterodimer with SPTLC2 or SPTLC3. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. Interacts with SPTSSA and SPTSSB; the interaction is direct. Interacts with ORMDL3 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO54695.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515LumenalSequence AnalysisAdd
    BLAST
    Topological domaini37 – 473437CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG003992.
    KOiK00654.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O54695-1 [UniParc]FASTAAdd to Basket

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    MAMAAEQWVL VEMVQALYEA PAYHLILEGI LILWIIRLVF SKTYKLQERS    50
    DLTAKEKEEL IEEWQPEPLV PPVSKNHPAL NYNIVSGPPT HNIVVNGKEC 100
    VNFASFNFLG LLANPRVKAA ALASLKKYGV GTCGPRGFYG TFDVHLDLEE 150
    RLAKFMRTEE AIIYSYGFST IASAIPAYSK RGDIVFVDSA ACFAIQKGLQ 200
    ASRSDIKLFK HNDVADLERL LKEQEIEDQK NPRKARVTRR FIVVEGLYMN 250
    TGTVCPLPEL VKLKYKYKAR IFLEESLSFG VLGEHGRGVT EHYGISIDDI 300
    DLISANMENA LASVGGFCCG RSFVVDHQRL SGQGYCFSAS LPPLLAAAAI 350
    EALNIMEENP GIFAVLKKKC QHIHKSLQGI SGLKVVGESL SPALHLQLEE 400
    STGSREKDVQ LLQEMVIHCM NEGIALTQAR YLDKEEKCLP PPSIRVVVTV 450
    EQTEEELERA ASTIREAAQA VLL 473
    Length:473
    Mass (Da):52,520
    Last modified:June 1, 1998 - v1
    Checksum:i1ABED2B60EC854B4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004831 mRNA. Translation: AAC53505.1.
    RefSeqiNP_001233688.1. NM_001246759.1.

    Genome annotation databases

    GeneIDi100689326.
    KEGGicge:100689326.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004831 mRNA. Translation: AAC53505.1 .
    RefSeqi NP_001233688.1. NM_001246759.1.

    3D structure databases

    ProteinModelPortali O54695.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O54695.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689326.
    KEGGi cge:100689326.

    Organism-specific databases

    CTDi 10558.

    Phylogenomic databases

    HOVERGENi HBG003992.
    KOi K00654.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis."
      Hanada K., Hara T., Nishijima M., Kuge O., Dickson R.C., Nagiec M.M.
      J. Biol. Chem. 272:32108-32114(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.
    2. "Localization, topology, and function of the LCB1 subunit of serine palmitoyltransferase in mammalian cells."
      Yasuda S., Nishijima M., Hanada K.
      J. Biol. Chem. 278:4176-4183(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MEMBRANE TOPOLOGY.

    Entry informationi

    Entry nameiSPTC1_CRIGR
    AccessioniPrimary (citable) accession number: O54695
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3