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O54694

- SPTC2_CRIGR

UniProt

O54694 - SPTC2_CRIGR

Protein

Serine palmitoyltransferase 2

Gene

SPTLC2

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate By similarity.By similarity

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. sphingolipid metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 2 (EC:2.3.1.50)
    Alternative name(s):
    Long chain base biosynthesis protein 2
    Short name:
    LCB 2
    Long chain base biosynthesis protein 2a
    Short name:
    LCB2a
    Serine-palmitoyl-CoA transferase 2
    Short name:
    SPT 2
    Gene namesi
    Name:SPTLC2
    Synonyms:LCB2
    OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
    Taxonomic identifieri10029 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Serine palmitoyltransferase 2PRO_0000163857Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei377 – 3771N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiO54694.

    Interactioni

    Subunit structurei

    Heterodimer with SPTLC1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1/SPTLC1, LCB2 (SPTLC2 or SPTLC3) and ssPT (SPTSSA or SPTSSB) By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO54694.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei65 – 8521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG002230.
    KOiK00654.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54694-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRPEPGGCCC RRPLRANGCV KNGEVRNGYV RSSTATAAAA GQIHHVTENG    50
    GLYKRPFNEV FEETPMLVAV LTYVGYGVLT LFGYLRDFLR HWRIEKCHHA 100
    TEREEQKDFV SLYQDFENFY TRNLYMRIRD NWNRPICSVP GARVDIMERQ 150
    SHDYNWSFKY TGNIIKGVIN MGSYNYLGFA RNTGSCQEAA AEVLKEYGAG 200
    VCSTRQEIGN LDKHEELEKL VARFLGVEAA MTYGMGFATN SMNIPALVGK 250
    GCLILSDELN HASLVLGARL SGATIRIFKH NNMQSLEKLL KDAIVYGQPR 300
    TRRPWKKILI LVEGIYSMEG SIVRLPEVIA LKKKYKAYLY LDEAHSIGAL 350
    GPSGRGVVDY FGLDPEDVDV MMGTFTKSFG ASGGYIGGKK ALIDYLRTHS 400
    HSAVYATSMS PPVMEQIITS MKCIMGQDGT SLGKECVQQL AENTKYFRRR 450
    LKEMGFIIYG NEDSPVVPLM LYMPAKIGAF GREMLKRNVG VVVVGFPATP 500
    IIESRARFCL SAAHTKEILD TALKEIDEVG DLLQLKYSRR RLVPLLDRPF 550
    DETTYEETED 560
    Length:560
    Mass (Da):62,882
    Last modified:June 1, 1998 - v1
    Checksum:iC835A5E0244878E6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004830 mRNA. Translation: AAC53504.1.
    RefSeqiNP_001233609.1. NM_001246680.1.

    Genome annotation databases

    GeneIDi100689415.
    KEGGicge:100689415.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004830 mRNA. Translation: AAC53504.1 .
    RefSeqi NP_001233609.1. NM_001246680.1.

    3D structure databases

    ProteinModelPortali O54694.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi O54694.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100689415.
    KEGGi cge:100689415.

    Organism-specific databases

    CTDi 9517.

    Phylogenomic databases

    HOVERGENi HBG002230.
    KOi K00654.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A mammalian homolog of the yeast LCB1 encodes a component of serine palmitoyltransferase, the enzyme catalyzing the first step in sphingolipid synthesis."
      Hanada K., Hara T., Nishijima M., Kuge O., Dickson R.C., Nagiec M.M.
      J. Biol. Chem. 272:32108-32114(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiSPTC2_CRIGR
    AccessioniPrimary (citable) accession number: O54694
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3