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O54692 (ZW10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere/kinetochore protein zw10 homolog
Gene names
Name:Zw10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum By similarity.

Subunit structure

Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1 and ZWINT By similarity.

Subcellular location

Cytoplasm By similarity. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Chromosomecentromerekinetochore By similarity. Cytoplasmcytoskeletonspindle By similarity. Note: Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm By similarity. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone By similarity.

Sequence similarities

Belongs to the ZW10 family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
ER-Golgi transport
Mitosis
Protein transport
Transport
   Cellular componentCentromere
Chromosome
Cytoplasm
Cytoskeleton
Endoplasmic reticulum
Kinetochore
Membrane
   DomainCoiled coil
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processER to Golgi vesicle-mediated transport

Inferred from sequence or structural similarity. Source: HGNC

establishment of mitotic spindle orientation

Inferred from electronic annotation. Source: Ensembl

mitotic cell cycle checkpoint

Inferred from sequence or structural similarity. Source: HGNC

mitotic metaphase plate congression

Inferred from electronic annotation. Source: Ensembl

mitotic sister chromatid segregation

Inferred from sequence or structural similarity. Source: HGNC

protein complex assembly

Inferred from sequence or structural similarity. Source: HGNC

protein localization to kinetochore

Inferred from electronic annotation. Source: Ensembl

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of exit from mitosis

Inferred from sequence or structural similarity. Source: HGNC

   Cellular_componentcondensed chromosome kinetochore

Inferred from electronic annotation. Source: UniProtKB-SubCell

endoplasmic reticulum

Inferred from sequence or structural similarity. Source: HGNC

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

kinetochore

Inferred from sequence or structural similarity. Source: HGNC

kinetochore microtubule

Inferred from sequence or structural similarity. Source: HGNC

nucleus

Inferred from sequence or structural similarity. Source: HGNC

spindle pole

Inferred from sequence or structural similarity. Source: HGNC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 779778Centromere/kinetochore protein zw10 homolog
PRO_0000184958

Regions

Region2 – 317316Interaction with RINT1 By similarity
Coiled coil14 – 130117 Potential

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue7771N6-acetyllysine By similarity

Experimental info

Sequence conflict1431E → A in BAE26337. Ref.1
Sequence conflict2811T → A in BAE26337. Ref.1
Sequence conflict2811T → A in BAE39941. Ref.1
Sequence conflict2921A → T in BAE39941. Ref.1
Sequence conflict4491T → K in BAE26337. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O54692 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5C02CF77FDF5B13B

FASTA77988,063
        10         20         30         40         50         60 
MASFVTEVLA HSGSLEKEDL GTRISRLTRR VEEIKGEVCN MISKKYSEFL PTMQSAQALV 

        70         80         90        100        110        120 
TQVDTLSNDI DQLKSRIETE VCRDLHISTV EFTNLKQQLE RDSVVLTLLK QLQEFSSAIE 

       130        140        150        160        170        180 
EYNSALAEKK YIPAARHLEE AQECLKLLKS RKCFDLKMLK SLSMELTVQK QNILYHLGED 

       190        200        210        220        230        240 
WQKLVVWKFP PAKDTSSLES CLQTELHLCT EQPEKEDMTP LPSISSVLLA FSILGELPTK 

       250        260        270        280        290        300 
LKSFGQMLLK YILKPLVTCP SLHAVIERQP SSVSICFESL TTDLEHPSPP EAFAKIRLVL 

       310        320        330        340        350        360 
EVLQKQLLDL PLDADLEIGK VPGIVLAEML GEGIWEDLSE CLIRNCLVYS IPTNSSKLQE 

       370        380        390        400        410        420 
YEEIIQSTEE FEKFLKEMRF LKGDTTDLLK YARNINSHFA NKKCQDVIVA ARNLMTSEIH 

       430        440        450        460        470        480 
NTVKIGPDCK EALPDLPSPD ADHKLQVQTV CKAQFTDAGN LEPETSLDPQ SFSLPTCRIS 

       490        500        510        520        530        540 
EAVKKLMELA YQTLLEATTS SDQCAVQLFY SVRNIFHLFH DVVPTYHKEN LRKLPQLAAI 

       550        560        570        580        590        600 
HHNNCMYIAH HLLTLGHQFR LRLAPILCDG TTTFVDLVPG FRRLGTECFL AQMQAQKGEL 

       610        620        630        640        650        660 
LERLSSARSF ANMDDEENYS AASKAVRQVL HQLRRLGIVW QDVLPVNIYC KAMGTLLNTA 

       670        680        690        700        710        720 
IAEMMSRITA LEDISTEDGD RLYSLCKTVM DEGPQVFAPL SDENKNKKYQ EEVPVYVSKW 

       730        740        750        760        770 
MPFKELMIML QASLQEIGDR WADGKGPLAT AFPSSEVKAL IRALFQNTER RAAALAKIK 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and DBA/2.
Tissue: Kidney and Mammary gland.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
[3]"Conservation of the centromere/kinetochore protein ZW10."
Starr D.A., Williams B.C., Li Z., Etemad-Moghadam B., Dawe R.K., Goldberg M.L.
J. Cell Biol. 138:1289-1301(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 522-779.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK145270 mRNA. Translation: BAE26337.1.
AK167251 mRNA. Translation: BAE39372.1.
AK167937 mRNA. Translation: BAE39941.1.
AK168479 mRNA. Translation: BAE40368.1.
BC012435 mRNA. Translation: AAH12435.1.
AF003951 mRNA. Translation: AAB88256.1.
RefSeqNP_036169.1. NM_012039.2.
UniGeneMm.24791.

3D structure databases

ProteinModelPortalO54692.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid205086. 8 interactions.
IntActO54692. 9 interactions.

PTM databases

PhosphoSiteO54692.

Proteomic databases

PaxDbO54692.
PRIDEO54692.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034803; ENSMUSP00000034803; ENSMUSG00000032264.
GeneID26951.
KEGGmmu:26951.
UCSCuc009piu.2. mouse.

Organism-specific databases

CTD9183.
MGIMGI:1349478. Zw10.

Phylogenomic databases

eggNOGNOG299329.
GeneTreeENSGT00390000016427.
HOGENOMHOG000007982.
HOVERGENHBG004603.
InParanoidO54692.
KOK11578.
OMAEHPSPSE.
OrthoDBEOG718KBR.
PhylomeDBO54692.
TreeFamTF105966.

Gene expression databases

BgeeO54692.
GenevestigatorO54692.

Family and domain databases

InterProIPR009361. RZZ-complex_Zw10.
[Graphical view]
PfamPF06248. Zw10. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304895.
PROO54692.
SOURCESearch...

Entry information

Entry nameZW10_MOUSE
AccessionPrimary (citable) accession number: O54692
Secondary accession number(s): Q3TIA5, Q3ULW1, Q921H3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot