O54666 (O54666_AMYMD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
RifA EMBL AAC01710.1

Submitted name:
Rifamycin polyketide synthase, type 1 EMBL CAA11035.1

Gene names

Name:

rifA EMBL AAC01710.1

Organism

Amycolatopsis mediterranei (Nocardia mediterranei) EMBL AAC01710.1

Taxonomic identifier

33910 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	4735 AA.
Sequence status	Complete.
Protein existence	Predicted

Ontologies

Keywords
Ligand	Phosphopantetheine RuleBase RU000721
Molecular function	Transferase RuleBase RU000693
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: InterPro cofactor binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 631	631	polyketide synthase loading domain EMBL AAC01710.1		PRO_5000053932
	Chain	632 – 2194	1563	3-AHBA CoA ligase module 1 EMBL AAC01710.1		PRO_5000053934
	Chain	632 – 1054	423	ketoacyl synthase EMBL AAC01710.1		PRO_5000053933
	Chain	1147 – 1456	310	methylmalonyl acyltransferase EMBL AAC01710.1		PRO_5000053935
	Chain	1467 – 1622	156	dehydratase EMBL AAC01710.1		PRO_5000053936
	Chain	1829 – 2075	247	ketoreductase EMBL AAC01710.1		PRO_5000053937
	Chain	2104 – 2178	75	acyl carrier protein EMBL AAC01710.1		PRO_5000053938
	Chain	2195 – 3171	977	polyketide synthase module 2 EMBL AAC01710.1		PRO_5000053940
	Chain	2195 – 2618	424	ketoacyl synthase EMBL AAC01710.1		PRO_5000053939
	Chain	2718 – 3022	305	malonyl acyltransferase EMBL AAC01710.1		PRO_5000053941
	Chain	3081 – 3155	75	acyl carrier protein EMBL AAC01710.1		PRO_5000053942
	Chain	3172 – 4735	1564	polyketide synthase module 3 EMBL AAC01710.1		PRO_5000053944
	Chain	3172 – 3596	425	ketoacyl synthase EMBL AAC01710.1		PRO_5000053943
	Chain	3697 – 4011	315	methylmalonyl acyltransferase EMBL AAC01710.1		PRO_5000053945
	Chain	4307 – 4548	242	ketoreductase EMBL AAC01710.1		PRO_5000053946
	Chain	4580 – 4654	75	acyl carrier protein EMBL AAC01710.1		PRO_5000053947

Sequences

	Sequence		Length	Mass (Da)
	O54666 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0B717178FB68C39C	FASTA	4,735	495,219
	10 20 30 40 50 60 MRTDLIKPLH VALLENATRF AGKPAFADDH RTVTYGDLEA RTRRLAGHLA GLGVRHGDRV 70 80 90 100 110 120 AICLGNRVST VESYFAILRA GAVGVPLNPG SATAELEHPL TDSGATVVVT DAAQAARLRL 130 140 150 160 170 180 APHVELLVTG DDVPEGAHSY DELALSEPAE PAADDLELDE PAWMFYTSGT TGRPKGVVST 190 200 210 220 230 240 QRNCLWSVAS CYVPFPGLSD QDRVLWPLPL FHSLSHIACV LSATVVGASV RIADGSSADD 250 260 270 280 290 300 VMRLIEAESS TFLAGVPTTY HHLVRAARQR GFSAPSLRIG LAGGAVLGAG LRSEFEETFG 310 320 330 340 350 360 VPLIDAYGST ETCGAITMNP PDGARVEGSC GLAVPGVDVR VVDPDTGLDV PAGEEGEVWV 370 380 390 400 410 420 SGPNVMLGYH NSPEATAAAM RDGWFRTGDL ARRDDAGYFT ICGRIKELII RGGANIHPGE 430 440 450 460 470 480 VEAVLRTVDG VADAAVGGVP HDTLGEVPVA YVIPGPTGFD PAALIEKCRE QLSAYKVPDR 490 500 510 520 530 540 ILEVAHIPRT ASGKIRRGLL TDEPAQLRYA ATEHEEQSRH ADESVAAALR ARLSGLDERA 550 560 570 580 590 600 QCELLEDLVR TQAADVLGQP VPDGRAFRDL GFTSLAIVEL RNRLTEHTGL WLPASAVFDH 610 620 630 640 650 660 PTPAALAARV RAELLGITQA VAEPVVAADP GEPIAIVGMA CRLPGGVASP EDLWRLVAER 670 680 690 700 710 720 VDAVSEFPGD RGWDLDSLID PDRERAGTSY VGQGGFLHDA GEFDAGFFGI SPREAVAMDP 730 740 750 760 770 780 QQRLLLETSW EALENAGVDP IALKGTDTGV FSGLMGQGYG SGAVAPELEG FVTTGVASSV 790 800 810 820 830 840 ASGRVSYVLG LEGPAVTVDT ACSSSLVAMH LAAQALRQGE CSMALAGGVT VMATPGSFVE 850 860 870 880 890 900 FSRQRALAPD GRCKAFAAAA DGTGWSEGVG VVVLERLSVA RERGHRILAV LRGSAVNQDG 910 920 930 940 950 960 ASNGLTAPNG LSQQRVIRRA LAAAGLAPSD VDVVEAHGTG TTLGDPIEAQ ALLATYGQER 970 980 990 1000 1010 1020 KQPLWLGSLK SNIGHAQAAA GVAGVIKMVQ ALRHETLPPT LHVDKPTLEV DWSAGAIELL 1030 1040 1050 1060 1070 1080 TEARAWPRNG RPRRAGVSSF GVSGTNAHLI LEEAPAEEPV AAPELPVVPL VVSARSTESL 1090 1100 1110 1120 1130 1140 SGQAERLASL LEGDVSLTEV AGALVSRRAV LDERAVVVAG SREEAVTGLR ALNTAGSGTP 1150 1160 1170 1180 1190 1200 GKVVWVFPGQ GTQWAGMGRE LLAESPVFAE RIAECAAALA PWIDWSLVDV LRGEGDLGRV 1210 1220 1230 1240 1250 1260 DVLQPACFAV MVGLAAVWES VGVRPDAVVG HSQGEIAAAC VSGALSLEDA AKVVALRSQA 1270 1280 1290 1300 1310 1320 IAAELSGRGG MASVALGEDD VVSRLVDGVE VAAVNGPSSV VIAGDAHALD ATLEILSGEG 1330 1340 1350 1360 1370 1380 IRVRRVAVDY ASHTRHVEDI RDTLAETLAG ISAQAPAVPF YSTVTSEWVR DAGVLDGGYW 1390 1400 1410 1420 1430 1440 YRNLRNQVRF GAAATALLEQ GHTVFVEVSA HPVTVQPLSE LTGDAIGTLR REDGGLRRLL 1450 1460 1470 1480 1490 1500 ASMGELFVRG IDVDWTAMVP AAGWVDLPTY AFEHRHYWLE PAEPASAGDP LLGTVVSTPG 1510 1520 1530 1540 1550 1560 SDRLTAVAQW SRRAQPWAVD GLVPNAALVE AAIRLGDLAG TPVVGELVVD APVVLPRRGS 1570 1580 1590 1600 1610 1620 REVQLIVGEP GEQRRRPIEV FSREADEPWT RHAHGTLAPA AAAVPEPAAA GDATDVTVAG 1630 1640 1650 1660 1670 1680 LRDADRYGIH PALLDAAVRT VVGDDLLPSV WTGVSLLASG ATAVTVTPTA TGLRLTDPAG 1690 1700 1710 1720 1730 1740 QPVLTVESVR GTPFVAEQGT TDALFRVDWP EIPLPTAETA DFLPYEATSA EATLSALQAW 1750 1760 1770 1780 1790 1800 LADPAETRLA VVTGDCTEPG AAAIWGLVRS AQSEHPGRIV LADLDDPAVL PAVVASGEPQ 1810 1820 1830 1840 1850 1860 VRVRNGVASV PRLTRVTPRQ DARPLDPEGT VLITGGTGTL GALTARHLVT AHGVRHLVLV 1870 1880 1890 1900 1910 1920 SRRGEAPELQ EELTALGASV AIAACDVADR AQLEAVLRAI PAEHPLTAVI HTAGVLDDGV 1930 1940 1950 1960 1970 1980 VTELTPDRLA TVRRPKVDAA RLLDELTREA DLAAFVLFSS AAGVLGNPGQ AGYAAANAEL 1990 2000 2010 2020 2030 2040 DALARQRNSL DLPAVSIAWG YWATVSGMTE HLGDADLRRN QRIGMSGLPA DEGMALLDAA 2050 2060 2070 2080 2090 2100 IATGGTLVAA KFDVAALRAT AKAGGPVPPL LRGLAPLPRR AAAKTASLTE RLAGLAETEQ 2110 2120 2130 2140 2150 2160 AAALLDLVRR HAAEVLGHSG AESVHSGRTF KDAGFDSLTA VELRNRLAAA TGLTLSPAMI 2170 2180 2190 2200 2210 2220 FDYPKPPALA DHLRAKLFGS AANRPAEIGT AAAEEPIAIV AMACRFPGGV HSPEDLWRLV 2230 2240 2250 2260 2270 2280 ADGADAVTEF PADRGWDTDR LYHEDPDHEG TTYVRHGAFL DDAAGFDAAF FGISPNEALA 2290 2300 2310 2320 2330 2340 MDPQQRLLLE TSWELFERAA IDPTTLAGQD IGVFAGVNSH DYSMRMHRAA GVEGFRLTGG 2350 2360 2370 2380 2390 2400 SASVLSGRVA YHFGVEGPAV TVDTACSSSL VALHMAVQAL QRGECSMALA GGVMVMGTVE 2410 2420 2430 2440 2450 2460 TFVEFSRQRG LAPDGRCKAF ADGADGTGWS EGVGLLLVER LSEAQRRGHQ VLAVVRGSAV 2470 2480 2490 2500 2510 2520 NSDGASNGLT APNGPSQQRV IRKALAAAGL STSDVDAVEA HGTGTTLGDP IEAEALLATY 2530 2540 2550 2560 2570 2580 GQNRETPLWL GSVKSNLGHT QAAAGVAGVI KMVMAMRHGV LPRTLHVDRP SSYVDWSAGA 2590 2600 2610 2620 2630 2640 VELLTEARDW VSNGHPRRAG VSSFGIGGTN AHVVLEEVAA PITTPQPEPA EFLVPVLVSA 2650 2660 2670 2680 2690 2700 RTAAGLRGQA GRLAAFLGDR TDVRVPDAAY ALATTRAQLD HRAVVLASDR AQLCADLAAF 2710 2720 2730 2740 2750 2760 GSGVVTGTPV DGKLAVLFTG QGSQWAGMGR ELAETFPVFR DAFEAACEAV DTHLRERPLR 2770 2780 2790 2800 2810 2820 EVVFDDSALL DQTMYTQGAL FAVETALFRL FESWGVRPGL LAGHSIGELA AAHVSGVLDL 2830 2840 2850 2860 2870 2880 ADAGELVAAR GRLMQALPAG GAMVAVQATE DEVAPLLDGT VCVAAVNGPD SVVLSGTEAA 2890 2900 2910 2920 2930 2940 VLAVADELAG RGRKTRRLAV SHAFHSPLME PMLDDFRAVA ERLTYRAGSL PVVSTLTGEL 2950 2960 2970 2980 2990 3000 AALDSPDYWV GQVRNAVRFS DAVTALGAQG ASTFLELGPG GALAAMALGT LGGPEQSCVA 3010 3020 3030 3040 3050 3060 TLRKNGAEVP DVLTALAELH VRGVGVDWTT VLDEPATAVG TVLPTYAFQH QRFWVDVDET 3070 3080 3090 3100 3110 3120 AAVSVTPPPA EPIVDRPVQD VLELVRESAA VVLGHRDAGS FDLDRSFKDH GFDSLSAVKL 3130 3140 3150 3160 3170 3180 RNRLRDFTGV ELPSTLIFDY PNPAVLADHL RAELLGERPA APAPVTRDVS DEPIAIVGMS 3190 3200 3210 3220 3230 3240 TRLPGGADSP EELWKLVAEG RDAVSGFPVD RGWDLDGLYH PDPAHAGTSY TRSGGFLHDA 3250 3260 3270 3280 3290 3300 AQFDAGLFGI SPREALAMDP QQRLLLETSW EALERAGVDP LSARGSDVGV FTGIVHHDYV 3310 3320 3330 3340 3350 3360 TRLREVPEDV QGYTMTGTAS SVASGRVAYV FGFEGPAVTV DTACSSSLVA MHLAAQALRQ 3370 3380 3390 3400 3410 3420 GECSMALAGG ATVMASPDAF LEFSRQRGLS ADGRCKAYAE GADGTGWAEG VGVVVLERLS 3430 3440 3450 3460 3470 3480 VARERGHRVL AVLRGSAVNQ DGASNGLTAP NGPSQQRVIR GALASAGLAP SDVDVVEGHG 3490 3500 3510 3520 3530 3540 TGTALGDPIE VQALLATYGQ EREQPLWLGS LKSNLGHTQA AAGVVGVIKM IMAMRHGVMP 3550 3560 3570 3580 3590 3600 ATLHVDERTS QVDWSAGAIE VLTEAREWPR TGRPRRAGVS SFGASGTNAH LIIEEGPAEE 3610 3620 3630 3640 3650 3660 AVDEEVASVV PLVVSARSAG SLAGQAGRLA AVLENESLAG VAGALVSGRA TLNERAVVIA 3670 3680 3690 3700 3710 3720 GSRDEAQDGL QALARGENAP GVVTGTAGKP GKVVWVFPGQ GSQWMGMGRD LLDSSPVFAA 3730 3740 3750 3760 3770 3780 RIKECAAALE QWTDWSLLDV LRGDADLLDR VDVVQPASFA MMVGLAAVWT SLGVTPDAVL 3790 3800 3810 3820 3830 3840 GHSQGEIAAA CVSGALSLDD AAKVVALRSQ AIAGELAGRG GMASVALSEE DAVARLTPWA 3850 3860 3870 3880 3890 3900 NRVEVAAVNS PSSVVIAGDA QALDEALEAL AGDGVRVRRV AVDYASHTRH VEAIAETLAK 3910 3920 3930 3940 3950 3960 TLAGIDARVP AIPFYSTVLG TWIEQAVVDA GYWYRNLRQQ VRFGPSVADL AGLGHTVFVE 3970 3980 3990 4000 4010 4020 ISAHPVLVQP LSEISDDAVV TGSLRRDDGG LRRLLASAAE LYVRGVAVDW TAAVPAAGWV 4030 4040 4050 4060 4070 4080 DLPTYAFDRR HFWLHEAETA EAAEGMDGEF WTAIEQSDVD SLAELLELVP EQRGALSTVV 4090 4100 4110 4120 4130 4140 PVLAQWRDRR RERSTAEKLR YQVTWQPLER EAAGVPGGRW LAVVPAGTTD ALLKELTGQG 4150 4160 4170 4180 4190 4200 LDIVRLEIEE ASRAQLAEQL RNVLAEHDLT GVLSLLALDG GPADAAEITA STLALVQALG 4210 4220 4230 4240 4250 4260 DTTTSAPLWC LTSGAVNIGI QDAVTAPAQA AVWGLGRAVA LERLDRWGGL VDLPAAIDAR 4270 4280 4290 4300 4310 4320 TAQALLGVLN GAAGEDQLAV RRSGVYRRRL VRKPVPESAT SRWEPRGTVL VTGGAEGLGR 4330 4340 4350 4360 4370 4380 HASVWLAQSG AERLIVTGTD GVDELTAELA EFGTTVEFCA DTDRDAIAQL VADSEVTAVV 4390 4400 4410 4420 4430 4440 HAADIAQTSS VDDTGVADLD EVFAAKVTTA VWLDQLFEDT PLDAFVVFSS IAGIWGGGGQ 4450 4460 4470 4480 4490 4500 GPAGAANAVL DALVEWRRAR GLKATSIAWG ALDQIGIGMD EAALAQLRRR GVIPMAPPLA 4510 4520 4530 4540 4550 4560 VTAMVQAVAG NEKAVAVADM DWAAFIPAFT SVRPSPLFAD LPEAKAILRA AQDDGEDGDT 4570 4580 4590 4600 4610 4620 ASSLADSLRA VPDAEQNRIL LKLVRGHAST VLGHSGAEGI GPRQAFQEVG FDSLAAVNLR 4630 4640 4650 4660 4670 4680 NSLHAATGLR LPATLIFDYP TPEALVGYLR VELLREADDG LDGREDDLRR VLAAVPFARF 4690 4700 4710 4720 4730 KEAGVLDTLL GLADTGTEPG TDAETTEAAP AADDAELIDA LDISGLVQRA LGQTS

References

[1]	"Biosynthesis of the ansamycin antibiotic rifamycin: deductions from the molecular analysis of the rif biosynthetic gene cluster of Amycolatopsis mediterranei S699." August P.R., Tang L., Yoon Y.J., Ning S., Muller R., Yu T.W., Taylor M., Hoffmann D., Kim C.G., Zhang X., Hutchinson C.R., Floss H.G. Chem. Biol. 5:69-79(1998) [PubMed: 9512878] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01710.1.
[2]	"Mutational analysis and reconstituted expression of the biosynthetic genes involved in the formation of 3-amino-5-hydroxybenzoic acid, the starter unit of rifamycin biosynthesis in amycolatopsis Mediterranei S699." Yu T.W., Muller R., Muller M., Zhang X., Draeger G., Kim C.G., Leistner E., Floss H.G. J. Biol. Chem. 276:12546-12555(2001) [PubMed: 11278540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01710.1.
[3]	"3-amino-5-hydroxybenzoic acid synthase, the terminal enzyme in the formation of the precursor of mC7N units in rifamycin and related antibiotics." Kim C.G., Yu T.W., Fryhle C.B., Handa S., Floss H.G. J. Biol. Chem. 273:6030-6040(1998) [PubMed: 9497318] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01710.1.
[4]	"Rifamycin insusceptibility: exploring the rif gene cluster of Amycolatopsis mediterranei S699." Yu T.-W., Pogosova-Agadjanyan E.L., Kuan L.-Y., Bai L., Tin A.M., Adman E., Floss H.G. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01710.1.
[5]	"Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei." Schupp T., Toupet C., Engel N., Goff S. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: LBG A3136 EMBL CAA11035.1.
[6]	Yu T., August P.R., Tang L., Yoon Y.J., Ning S., Mueller R., Taylor M., Kim C., Zhang X., Pogosova-Agadjanyan E.L., Tin A.M., Hutchinson C.R., Floss H.G. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: S699 EMBL AAC01710.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF040570 Genomic DNA. Translation: AAC01710.1. AJ223012 Genomic DNA. Translation: CAA11035.1.
PIR	T17463.
3D structure databases
HSSP	HSSP built from PDB template 1LCI based on UniProtKB P08659.
ProteinModelPortal	O54666.
SMR	O54666. Positions 626-1480, 3168-4035, 4562-4653.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001227. Ac_transferase_dom. IPR009081. Acyl_carrier_prot-like. IPR014043. Acyl_transferase. IPR016035. Acyl_Trfase/lysoPLipase. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. IPR000794. Beta-ketoacyl_synthase. IPR018201. Ketoacyl_synth_AS. IPR014031. Ketoacyl_synth_C. IPR014030. Ketoacyl_synth_N. IPR016036. Malonyl_transacylase_ACP-bd. IPR016040. NAD(P)-bd_dom. IPR006163. Phsphopanteth-bd. IPR020842. PKS/FAS_KR. IPR020801. PKS_acyl_transferase. IPR020841. PKS_Beta-ketoAc_synthase_dom. IPR020807. PKS_dehydratase. IPR013968. PKS_KR. IPR020806. PKS_PP-bd. IPR006162. PPantetheine_attach_site. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.366.10. Ac_transferase_reg. 6 hits. G3DSA:1.10.1200.10. ACP_like. 4 hits. G3DSA:3.40.50.720. NAD(P)-bd. 2 hits. G3DSA:3.40.47.10. Thiolase-like_subgr. 6 hits.
PANTHER	PTHR11712. Ketoacyl_synth. 1 hit.
Pfam	PF00698. Acyl_transf_1. 3 hits. PF00501. AMP-binding. 1 hit. PF00109. ketoacyl-synt. 3 hits. PF02801. Ketoacyl-synt_C. 3 hits. PF08659. KR. 2 hits. PF00550. PP-binding. 4 hits. [Graphical view]
SMART	SM00827. PKS_AT. 3 hits. SM00826. PKS_DH. 1 hit. SM00822. PKS_KR. 2 hits. SM00825. PKS_KS. 3 hits. SM00823. PKS_PP. 4 hits. [Graphical view]
SUPFAM	SSF47336. ACP_like. 4 hits. SSF52151. Acyl_Trfase/lysoPlipase. 3 hits. SSF55048. Malonyl_transacylase_ACP-bd. 3 hits. SSF53901. Thiolase-like. 3 hits.
PROSITE	PS50075. ACP_DOMAIN. 4 hits. PS00455. AMP_BINDING. 1 hit. PS00606. B_KETOACYL_SYNTHASE. 3 hits. PS00012. PHOSPHOPANTETHEINE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O54666_AMYMD

Accession

Primary (citable) accession number: O54666

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	December 14, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)