Reviewed, UniProtKB/Swiss-Prot O54571 (RSMG_STRCO)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA small subunit methyltransferase G
    EC=2.1.1.-
Alternative name(s):
    16S rRNA 7-methylguanosine methyltransferase
      Short name=16S rRNA m7G methyltransferase
    Glucose-inhibited division protein B

Gene names

Name:	rsmG
Synonyms:	gidB
Ordered Locus Names:	SCO3885
ORF Names:	STH24.07

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA. Ref.4
Subcellular location	Cytoplasm Potential.
Disruption phenotype	Confers low-level streptomycin resistance and a greater ability to produce the antibiotic actinorhodin. Ref.4
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: InterPro rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribosomal RNA small subunit methyltransferase G HAMAP MF_00074		PRO_0000184349

Sequences

	Sequence		Length	Mass (Da)
	O54571-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2297957AB2C8C011	FASTA	239	25,647
	10 20 30 40 50 60 MSEAAELPPV PEQARDVFGD RYADAVRYAE LLAEAGVKRG LIGPREVPRL WERHLLNCAV 70 80 90 100 110 120 LSEVVPEGVT VCDVGSGAGL PGIPLALVRE DLKITLLEPL LRRTNFLTEV VELLGLDHVT 130 140 150 160 170 180 VVRGRAEEVM GKLPPVHVVT ARAVAPLDRL ATWGIPLLRP YGEMLALKGD TAEEELKAAT 190 200 210 220 230 AALSKLGAEQ TSILHVGEGV VSPLSTVVRV EVGESPGGVR FAAKRAKAAR TGRTRRRRG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene organization in the trxB/A-oriC region of the Streptomyces coelicolor 3A(2) chromosome and comparison with other bacteria." Gal-Mor O., Borovok I., Av-Gay Y., Cohen G., Aharonowitz Y. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"Partitioning of the linear chromosome during sporulation of Streptomyces coelicolor A3(2) involves an oriC-linked parAB locus." Kim H.-J., Calcutt M.J., Schmidt F.J., Chater K.F. J. Bacteriol. 182:1313-1320(2000) [PubMed: 10671452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A3(2).
[3]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[4]	"Mutations in rsmG, encoding a 16S rRNA methyltransferase, result in low-level streptomycin resistance and antibiotic overproduction in Streptomyces coelicolor A3(2)." Nishimura K., Hosaka T., Tokuyama S., Okamoto S., Ochi K. J. Bacteriol. 189:3876-3883(2007) [PubMed: 17384192] [Abstract] Cited for: FUNCTION AS A M7G METHYLTRANSFERASE, DISRUPTION PHENOTYPE. Strain: A3(2) / 1147.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF031590 Genomic DNA. Translation: AAC03485.1. AF187159 Genomic DNA. Translation: AAF16006.1. Different initiation. AL939118 Genomic DNA. Translation: CAB42703.1.
PIR	T36572.
RefSeq	NP_628071.1.
3D structure databases
HSSP	HSSP built from PDB template 1JSX based on UniProtKB P17113.
ModBase	Search...
Genome annotation databases
GeneID	1099321.
GenomeReviews	Gene locus SCO3885 in contig AL645882_GR.
KEGG	sco:SCO3885.
NMPDR	fig|100226.1.peg.3842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O54571.
OMA	DRHILNC.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO3885-MON.
Family and domain databases
HAMAP	MF_00074. [Tree]
InterPro	IPR003682. GidB. [Graphical view]
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
ProDom	PD004441. GidB. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00138. gidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_STRCO

Accession

Primary (citable) accession number: O54571
Secondary accession number(s): Q9RFM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)