O54479 (PARE_CAUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA topoisomerase 4 subunit B
EC=5.99.1.-

Alternative name(s):
Topoisomerase IV subunit B

Gene names

Name:	parE
Ordered Locus Names:	CC_1974

Organism

Caulobacter crescentus (Caulobacter vibrioides)

Taxonomic identifier

155892 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Caulobacterales › Caulobacteraceae › Caulobacter

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule By similarity.
Subunit structure	Composed of two subunits: parC and parE.
Sequence similarities	Belongs to the type II topoisomerase family. Contains 1 Toprim domain.
Sequence caution	The sequence AAC38044.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF14340.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	DNA topoisomerase 4 subunit B		PRO_0000145426
Regions
	Domain	547 – 588	42	Toprim

Sequences

	Sequence		Length	Mass (Da)
	O54479 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 7738A20961444C4D	FASTA	695	76,164
	10 20 30 40 50 60 MSSSDKIPSL FGDDDALAPV PAAPFKASVE PRVEPTPRPI PPPPPSKTAS APGEYSAADI 70 80 90 100 110 120 EVLEGLEPVR KRPGMYIGGT DERALHHLFA EVLDNSMDEA VAGFAKTIEV KLDADGFLSV 130 140 150 160 170 180 KDDGRGMPVD PHPKYPGKSA LEVIMTVLHA GGKFTGKAYE TSGGLHGVGA SVVNALSERV 190 200 210 220 230 240 EVTVWRDGFE HLQVFSRGKP LGPIQQVAPS KKKGTMVRFK PDDEIFGEGT NFKPARLYRM 250 260 270 280 290 300 ARSKAYLFRG VQIKWSCDPS RIHDQTPPEA TFHFPNGLAD FLAERTKGLT TITPESFAGR 310 320 330 340 350 360 IERQGEAGAV EWAVTWTPQG FGEHDGFMQS YCNTVPTPEG GTHESGFRAA LTRGLKAYAE 370 380 390 400 410 420 LKGEKRGTII TADDVVAQAG ALISVFIKNP EFQGQTKEKL STSEAQRFVE ASLRDPFDLW 430 440 450 460 470 480 LSSSPKNAQA LLEFVIERAE ERLKRRKDKE VSRASATRKL RLPGKLADCA GSAVDGAELF 490 500 510 520 530 540 IVEGDSAGGS AKQARDRKYQ AILPLRGKIL NVASASGEKF TANKELSDLM LALGAQAGAK 550 560 570 580 590 600 YREEDLRYER IIIMTDADVD GAHIASLLIT FFYRTMPELI RGGHLFLALP PLYRLAHGGK 610 620 630 640 650 660 SEYARDDAHK EELLATVFKG KKPEIGRFKG LGEMMASQLK ETTMDPKKRT LARVTLPRHE 670 680 690 ESVEDLVETL MGRKPELRFR FIQENAEFAS ADLDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Ward D.V., Newton A. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 19089 / CB15.
[2]	"Complete genome sequence of Caulobacter crescentus." Nierman W.C., Feldblyum T.V., Laub M.T., Paulsen I.T., Nelson K.E., Eisen J.A., Heidelberg J.F., Alley M.R.K., Ohta N., Maddock J.R., Potocka I., Nelson W.C., Newton A., Stephens C., Phadke N.D., Ely B., DeBoy R.T., Dodson R.J. , Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Smit J., Craven M.B., Khouri H.M., Shetty J., Berry K.J., Utterback T.R., Tran K., Wolf A.M., Vamathevan J.J., Ermolaeva M.D., White O., Salzberg S.L., Venter J.C., Shapiro L., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 98:4136-4141(2001) [PubMed: 11259647] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19089 / CB15.
[3]	"Requirement of topoisomerase IV parC and parE genes for cell cycle progression and developmental regulation in Caulobacter crescentus." Ward D.V., Newton A. Mol. Microbiol. 26:897-910(1997) [PubMed: 9426128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-695. Strain: ATCC 19089 / CB15.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U86303 Genomic DNA. Translation: AAF14340.1. Different initiation. AE005673 Genomic DNA. Translation: AAK23949.1. U94697 Genomic DNA. Translation: AAC38044.1. Different initiation.
PIR	A87494.
RefSeq	NP_420781.1. NC_002696.2.
3D structure databases
ProteinModelPortal	O54479.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	942751.
GenomeReviews	Gene locus CC_1974 in contig AE005673_GR.
KEGG	ccr:CC_1974.
NMPDR	fig|190650.1.peg.1961.
PATRIC	21300960. VBICauCre124313_1991.
TIGR	CC_1974.
Phylogenomic databases
HOGENOM	HBG304889.
OMA	QARDKEF.
ProtClustDB	PRK05559.
Family and domain databases
InterPro	IPR003594. ATPase-like_ATP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. IPR005737. TopoIV_B_Gneg. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
KO	K02622.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01055. ParE_Gneg. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARE_CAUCR

Accession

Primary (citable) accession number: O54479

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	April 27, 2001
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program