Reviewed, UniProtKB/Swiss-Prot O54439 (ACP1_PSEAE)

Names and origin

Protein names

Recommended name:
    Acyl carrier protein 1
      Short name=ACP 1

Gene names

Name:	acpP1
Synonyms:	acpP
Ordered Locus Names:	PA2966

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	78 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Carrier of the growing fatty acid chain in fatty acid biosynthesis By similarity. HAMAP MF_01217
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01217
Subcellular location	Cytoplasm By similarity HAMAP MF_01217.
Post-translational modification	4'-phosphopantetheine is transferred from CoA to a specific serine of apo-ACP by acpS. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group By similarity. HAMAP MF_01217
Sequence similarities	Contains 1 acyl carrier domain.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Phosphopantetheine
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl carrier activity Inferred from electronic annotation. Source: HAMAP cofactor binding Inferred from electronic annotation. Source: InterPro phosphopantetheine binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 78

77

Acyl carrier protein 1 HAMAP MF_01217

PRO_0000180167

Amino acid modifications

Modified residue

37

1

O-(pantetheine 4'-phosphoryl)serine By similarity

Experimental info

Sequence conflict

76

1

H → P in AAB94392. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

78

Helix Strand Turn

Details...

Helix

4 – 14

11

Helix

36 – 50

15

Helix

56 – 59

4

Helix

66 – 75

10

Sequences

	Sequence		Length	Mass (Da)
	O54439-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 77F95B04F25E1348	FASTA	78	8,741
	10 20 30 40 50 60 MSTIEERVKK IVAEQLGVKE EEVTNSASFV EDLGADSLDT VELVMALEEE FETEIPDEKA 70 EKITTVQEAI DYIVAHQQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (fabD)." Kutchma A.J., Hoang T.T., Schweizer H.P. J. Bacteriol. 181:5498-5504(1999) [PubMed: 10464226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U91631 Genomic DNA. Translation: AAB94392.1.
AE004091 Genomic DNA. Translation: AAG06354.1.

PIR

A83276.
T12021.

RefSeq

NP_251656.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OP7	model	-	A	1-78	[»]

SMR

O54439. Positions 2-77.

ModBase

Search...

Genome annotation databases

GeneID

879895.

GenomeReviews

Gene locus PA2966 in contig AE004091_GR.

KEGG

pae:PA2966.

Organism-specific databases

PseudoCAP

PA2966.

CMR

Search...

Phylogenomic databases

HOGENOM

HBG755218.

OMA

IDYITEH.

Enzyme and pathway databases

BioCyc

PAER208964:PA2966-MONOMER.

Family and domain databases

HAMAP

MF_01217. Acyl_carrier.
[Tree]

InterPro

IPR003231. Acyl_carrier.
IPR009081. Acyl_carrier_prot-like.
IPR006163. Phsphopanteth_bd.
IPR006162. PPantetheine_attach_site.
[Graphical view]

Pfam

PF00550. PP-binding. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00517. acyl_carrier. 1 hit.

PROSITE

PS50075. ACP_DOMAIN. 1 hit.
PS00012. PHOSPHOPANTETHEINE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ACP1_PSEAE

Accession

Primary (citable) accession number: O54439

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 80 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)