O54438 (FABG_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG
EC=1.1.1.100

Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase

Gene names

Name:	fabG
Ordered Locus Names:	PA2967

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis By similarity.
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	3-oxoacyl-[acyl-carrier-protein] reductase FabG		PRO_0000054678
Regions
	Nucleotide binding	12 – 15	4	NADP By similarity
	Nucleotide binding	62 – 63	2	NADP By similarity
	Nucleotide binding	154 – 158	5	NADP By similarity
Sites
	Active site	154	1	Proton acceptor By similarity
	Binding site	37	1	NADP By similarity
	Binding site	89	1	NADP; via carbonyl oxygen By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	187	1	NADP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54438 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: AB83A2B95027445B	FASTA	247	25,585
	10 20 30 40 50 60 MSLQGKVALV TGASRGIGQA IALELGRLGA VVIGTATSAS GAEKIAETLK ANGVEGAGLV 70 80 90 100 110 120 LDVSSDESVA ATLEHIQQHL GQPLIVVNNA GITRDNLLVR MKDDEWFDVV NTNLNSLYRL 130 140 150 160 170 180 SKAVLRGMTK ARWGRIINIG SVVGAMGNAG QTNYAAAKAG LEGFTRALAR EVGSRAITVN 190 200 210 220 230 240 AVAPGFIDTD MTRELPEAQR EALLGQIPLG RLGQAEEIAK VVGFLASDGA AYVTGATVPV NGGMYMS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (fabD)." Kutchma A.J., Hoang T.T., Schweizer H.P. J. Bacteriol. 181:5498-5504(1999) [PubMed: 10464226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U91631 Genomic DNA. Translation: AAB94395.1. AE004091 Genomic DNA. Translation: AAG06355.1.
PIR	T12020.
RefSeq	NP_251657.1. NC_002516.2.
3D structure databases
ProteinModelPortal	O54438.
SMR	O54438. Positions 2-246.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880433.
GenomeReviews	Gene locus PA2967 in contig AE004091_GR.
KEGG	pae:PA2967.
PATRIC	19840487. VBIPseAer58763_3113.
Organism-specific databases
PseudoCAP	PA2967.
Phylogenomic databases
HOGENOM	HBG750976.
OMA	DNLMLRM.
ProtClustDB	PRK05653.
Enzyme and pathway databases
BioCyc	PAER208964:PA2967-MONOMER.
Family and domain databases
InterPro	IPR011284. 3oxo_ACP_reduc. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00059.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG_PSEAE

Accession

Primary (citable) accession number: O54438

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program