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O54438

- FABG_PSEAE

UniProt

O54438 - FABG_PSEAE

Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

    Catalytic activityi

    (3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei37 – 371NADPBy similarity
    Binding sitei89 – 891NADP; via carbonyl oxygenBy similarity
    Binding sitei141 – 1411SubstrateBy similarity
    Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation
    Binding sitei187 – 1871NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 154NADPBy similarity
    Nucleotide bindingi62 – 632NADPBy similarity
    Nucleotide bindingi154 – 1585NADPBy similarity

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
    2. NAD binding Source: InterPro
    3. NADP binding Source: UniProtKB

    GO - Biological processi

    1. fatty acid elongation Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
    Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase
    Beta-Ketoacyl-acyl carrier protein reductase
    Beta-ketoacyl-ACP reductase
    Gene namesi
    Name:fabG
    Ordered Locus Names:PA2967
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA2967.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2472473-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054678Add
    BLAST

    Proteomic databases

    PRIDEiO54438.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi208964.PA2967.

    Structurei

    Secondary structure

    1
    247
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni2 – 54
    Beta strandi7 – 126
    Helixi16 – 2712
    Beta strandi31 – 388
    Helixi39 – 5012
    Turni51 – 533
    Beta strandi56 – 605
    Helixi66 – 7914
    Beta strandi85 – 884
    Helixi98 – 1003
    Helixi103 – 1053
    Helixi106 – 13126
    Beta strandi134 – 1396
    Helixi142 – 1465
    Helixi147 – 17226
    Helixi173 – 1753
    Beta strandi177 – 1848
    Beta strandi186 – 1894
    Turni190 – 1945
    Helixi197 – 2059
    Helixi215 – 22612
    Helixi228 – 2303
    Beta strandi237 – 2415

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AFNX-ray2.30A/B/C/D1-247[»]
    4AG3X-ray1.80A/B/C/D1-247[»]
    4BNTX-ray2.30A/B/C/D1-247[»]
    4BNUX-ray2.00A/B/C/D1-247[»]
    4BNVX-ray2.50A/B/C/D1-247[»]
    4BNWX-ray1.60A/B/C/D1-247[»]
    4BNXX-ray2.30A/B/C/D1-247[»]
    4BNYX-ray1.80A/B/C/D1-247[»]
    4BNZX-ray2.50A/B/C/D1-247[»]
    4BO0X-ray2.40A/B/C/D1-247[»]
    4BO1X-ray2.20A/B/C/D1-247[»]
    4BO2X-ray1.90A/B/C/D1-247[»]
    4BO3X-ray2.50A/B/C/D1-247[»]
    4BO4X-ray2.70A/B/C/D1-247[»]
    4BO5X-ray2.60A/B/C/D1-247[»]
    4BO6X-ray2.80A/B/C/D1-247[»]
    4BO7X-ray2.60A/B/C/D1-247[»]
    4BO8X-ray2.70A/B/C/D1-247[»]
    4BO9X-ray2.90A/B/C/D1-247[»]
    ProteinModelPortaliO54438.
    SMRiO54438. Positions 1-247.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1028.
    KOiK00059.
    OMAiDEFGAID.
    OrthoDBiEOG6N3CR8.
    PhylomeDBiO54438.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view]
    PfamiPF00106. adh_short. 1 hit.
    [Graphical view]
    PRINTSiPR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEiPS00061. ADH_SHORT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O54438-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLQGKVALV TGASRGIGQA IALELGRLGA VVIGTATSAS GAEKIAETLK    50
    ANGVEGAGLV LDVSSDESVA ATLEHIQQHL GQPLIVVNNA GITRDNLLVR 100
    MKDDEWFDVV NTNLNSLYRL SKAVLRGMTK ARWGRIINIG SVVGAMGNAG 150
    QTNYAAAKAG LEGFTRALAR EVGSRAITVN AVAPGFIDTD MTRELPEAQR 200
    EALLGQIPLG RLGQAEEIAK VVGFLASDGA AYVTGATVPV NGGMYMS 247
    Length:247
    Mass (Da):25,585
    Last modified:June 1, 1998 - v1
    Checksum:iAB83A2B95027445B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U91631 Genomic DNA. Translation: AAB94395.1.
    AE004091 Genomic DNA. Translation: AAG06355.1.
    PIRiT12020.
    RefSeqiNP_251657.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG06355; AAG06355; PA2967.
    GeneIDi880433.
    KEGGipae:PA2967.
    PATRICi19840487. VBIPseAer58763_3113.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U91631 Genomic DNA. Translation: AAB94395.1 .
    AE004091 Genomic DNA. Translation: AAG06355.1 .
    PIRi T12020.
    RefSeqi NP_251657.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AFN X-ray 2.30 A/B/C/D 1-247 [» ]
    4AG3 X-ray 1.80 A/B/C/D 1-247 [» ]
    4BNT X-ray 2.30 A/B/C/D 1-247 [» ]
    4BNU X-ray 2.00 A/B/C/D 1-247 [» ]
    4BNV X-ray 2.50 A/B/C/D 1-247 [» ]
    4BNW X-ray 1.60 A/B/C/D 1-247 [» ]
    4BNX X-ray 2.30 A/B/C/D 1-247 [» ]
    4BNY X-ray 1.80 A/B/C/D 1-247 [» ]
    4BNZ X-ray 2.50 A/B/C/D 1-247 [» ]
    4BO0 X-ray 2.40 A/B/C/D 1-247 [» ]
    4BO1 X-ray 2.20 A/B/C/D 1-247 [» ]
    4BO2 X-ray 1.90 A/B/C/D 1-247 [» ]
    4BO3 X-ray 2.50 A/B/C/D 1-247 [» ]
    4BO4 X-ray 2.70 A/B/C/D 1-247 [» ]
    4BO5 X-ray 2.60 A/B/C/D 1-247 [» ]
    4BO6 X-ray 2.80 A/B/C/D 1-247 [» ]
    4BO7 X-ray 2.60 A/B/C/D 1-247 [» ]
    4BO8 X-ray 2.70 A/B/C/D 1-247 [» ]
    4BO9 X-ray 2.90 A/B/C/D 1-247 [» ]
    ProteinModelPortali O54438.
    SMRi O54438. Positions 1-247.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA2967.

    Proteomic databases

    PRIDEi O54438.

    Protocols and materials databases

    DNASUi 880433.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG06355 ; AAG06355 ; PA2967 .
    GeneIDi 880433.
    KEGGi pae:PA2967.
    PATRICi 19840487. VBIPseAer58763_3113.

    Organism-specific databases

    PseudoCAPi PA2967.

    Phylogenomic databases

    eggNOGi COG1028.
    KOi K00059.
    OMAi DEFGAID.
    OrthoDBi EOG6N3CR8.
    PhylomeDBi O54438.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR011284. 3oxo_ACP_reduc.
    IPR002198. DH_sc/Rdtase_SDR.
    IPR002347. Glc/ribitol_DH.
    IPR016040. NAD(P)-bd_dom.
    IPR020904. Sc_DH/Rdtase_CS.
    [Graphical view ]
    Pfami PF00106. adh_short. 1 hit.
    [Graphical view ]
    PRINTSi PR00081. GDHRDH.
    PR00080. SDRFAMILY.
    TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
    PROSITEi PS00061. ADH_SHORT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (fabD)."
      Kutchma A.J., Hoang T.T., Schweizer H.P.
      J. Bacteriol. 181:5498-5504(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiFABG_PSEAE
    AccessioniPrimary (citable) accession number: O54438
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3