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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37NADPBy similarity1
Binding sitei89NADP; via carbonyl oxygenBy similarity1
Binding sitei141SubstrateBy similarity1
Active sitei154Proton acceptorPROSITE-ProRule annotation1
Binding sitei187NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15NADPBy similarity4
Nucleotide bindingi62 – 63NADPBy similarity2
Nucleotide bindingi154 – 158NADPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:PA2967
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA2967.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000546781 – 2473-oxoacyl-[acyl-carrier-protein] reductase FabGAdd BLAST247

Proteomic databases

PaxDbiO54438.
PRIDEiO54438.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi208964.PA2967.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni2 – 5Combined sources4
Beta strandi7 – 12Combined sources6
Helixi16 – 27Combined sources12
Beta strandi31 – 38Combined sources8
Helixi39 – 50Combined sources12
Turni51 – 53Combined sources3
Beta strandi56 – 60Combined sources5
Helixi66 – 79Combined sources14
Beta strandi85 – 88Combined sources4
Helixi98 – 100Combined sources3
Helixi103 – 105Combined sources3
Helixi106 – 131Combined sources26
Beta strandi134 – 139Combined sources6
Helixi142 – 146Combined sources5
Helixi147 – 172Combined sources26
Helixi173 – 175Combined sources3
Beta strandi177 – 184Combined sources8
Beta strandi186 – 189Combined sources4
Turni190 – 194Combined sources5
Helixi197 – 205Combined sources9
Helixi215 – 226Combined sources12
Helixi228 – 230Combined sources3
Beta strandi237 – 241Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AFNX-ray2.30A/B/C/D1-247[»]
4AG3X-ray1.80A/B/C/D1-247[»]
4BNTX-ray2.30A/B/C/D1-247[»]
4BNUX-ray2.00A/B/C/D1-247[»]
4BNVX-ray2.50A/B/C/D1-247[»]
4BNWX-ray1.60A/B/C/D1-247[»]
4BNXX-ray2.30A/B/C/D1-247[»]
4BNYX-ray1.80A/B/C/D1-247[»]
4BNZX-ray2.50A/B/C/D1-247[»]
4BO0X-ray2.40A/B/C/D1-247[»]
4BO1X-ray2.20A/B/C/D1-247[»]
4BO2X-ray1.90A/B/C/D1-247[»]
4BO3X-ray2.50A/B/C/D1-247[»]
4BO4X-ray2.70A/B/C/D1-247[»]
4BO5X-ray2.60A/B/C/D1-247[»]
4BO6X-ray2.80A/B/C/D1-247[»]
4BO7X-ray2.60A/B/C/D1-247[»]
4BO8X-ray2.70A/B/C/D1-247[»]
4BO9X-ray2.90A/B/C/D1-247[»]
ProteinModelPortaliO54438.
SMRiO54438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
InParanoidiO54438.
KOiK00059.
OMAiMKDEDWN.
PhylomeDBiO54438.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54438-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLQGKVALV TGASRGIGQA IALELGRLGA VVIGTATSAS GAEKIAETLK
60 70 80 90 100
ANGVEGAGLV LDVSSDESVA ATLEHIQQHL GQPLIVVNNA GITRDNLLVR
110 120 130 140 150
MKDDEWFDVV NTNLNSLYRL SKAVLRGMTK ARWGRIINIG SVVGAMGNAG
160 170 180 190 200
QTNYAAAKAG LEGFTRALAR EVGSRAITVN AVAPGFIDTD MTRELPEAQR
210 220 230 240
EALLGQIPLG RLGQAEEIAK VVGFLASDGA AYVTGATVPV NGGMYMS
Length:247
Mass (Da):25,585
Last modified:June 1, 1998 - v1
Checksum:iAB83A2B95027445B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91631 Genomic DNA. Translation: AAB94395.1.
AE004091 Genomic DNA. Translation: AAG06355.1.
PIRiT12020.
RefSeqiNP_251657.1. NC_002516.2.
WP_003091137.1. NZ_ASJY01000489.1.

Genome annotation databases

EnsemblBacteriaiAAG06355; AAG06355; PA2967.
GeneIDi880433.
KEGGipae:PA2967.
PATRICi19840487. VBIPseAer58763_3113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91631 Genomic DNA. Translation: AAB94395.1.
AE004091 Genomic DNA. Translation: AAG06355.1.
PIRiT12020.
RefSeqiNP_251657.1. NC_002516.2.
WP_003091137.1. NZ_ASJY01000489.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AFNX-ray2.30A/B/C/D1-247[»]
4AG3X-ray1.80A/B/C/D1-247[»]
4BNTX-ray2.30A/B/C/D1-247[»]
4BNUX-ray2.00A/B/C/D1-247[»]
4BNVX-ray2.50A/B/C/D1-247[»]
4BNWX-ray1.60A/B/C/D1-247[»]
4BNXX-ray2.30A/B/C/D1-247[»]
4BNYX-ray1.80A/B/C/D1-247[»]
4BNZX-ray2.50A/B/C/D1-247[»]
4BO0X-ray2.40A/B/C/D1-247[»]
4BO1X-ray2.20A/B/C/D1-247[»]
4BO2X-ray1.90A/B/C/D1-247[»]
4BO3X-ray2.50A/B/C/D1-247[»]
4BO4X-ray2.70A/B/C/D1-247[»]
4BO5X-ray2.60A/B/C/D1-247[»]
4BO6X-ray2.80A/B/C/D1-247[»]
4BO7X-ray2.60A/B/C/D1-247[»]
4BO8X-ray2.70A/B/C/D1-247[»]
4BO9X-ray2.90A/B/C/D1-247[»]
ProteinModelPortaliO54438.
SMRiO54438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA2967.

Proteomic databases

PaxDbiO54438.
PRIDEiO54438.

Protocols and materials databases

DNASUi880433.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06355; AAG06355; PA2967.
GeneIDi880433.
KEGGipae:PA2967.
PATRICi19840487. VBIPseAer58763_3113.

Organism-specific databases

PseudoCAPiPA2967.

Phylogenomic databases

eggNOGiENOG4105CHR. Bacteria.
ENOG410XNW1. LUCA.
InParanoidiO54438.
KOiK00059.
OMAiMKDEDWN.
PhylomeDBiO54438.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFABG_PSEAE
AccessioniPrimary (citable) accession number: O54438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.