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O54438

- FABG_PSEAE

UniProt

O54438 - FABG_PSEAE

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Protein

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Gene

fabG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.By similarity

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371NADPBy similarity
Binding sitei89 – 891NADP; via carbonyl oxygenBy similarity
Binding sitei141 – 1411SubstrateBy similarity
Active sitei154 – 1541Proton acceptorPROSITE-ProRule annotation
Binding sitei187 – 1871NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 154NADPBy similarity
Nucleotide bindingi62 – 632NADPBy similarity
Nucleotide bindingi154 – 1585NADPBy similarity

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB
  2. NAD binding Source: InterPro
  3. NADP binding Source: UniProtKB

GO - Biological processi

  1. fatty acid elongation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase FabG (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Beta-Ketoacyl-acyl carrier protein reductase
Beta-ketoacyl-ACP reductase
Gene namesi
Name:fabG
Ordered Locus Names:PA2967
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA2967.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2472473-oxoacyl-[acyl-carrier-protein] reductase FabGPRO_0000054678Add
BLAST

Proteomic databases

PRIDEiO54438.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi208964.PA2967.

Structurei

Secondary structure

1
247
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni2 – 54Combined sources
Beta strandi7 – 126Combined sources
Helixi16 – 2712Combined sources
Beta strandi31 – 388Combined sources
Helixi39 – 5012Combined sources
Turni51 – 533Combined sources
Beta strandi56 – 605Combined sources
Helixi66 – 7914Combined sources
Beta strandi85 – 884Combined sources
Helixi98 – 1003Combined sources
Helixi103 – 1053Combined sources
Helixi106 – 13126Combined sources
Beta strandi134 – 1396Combined sources
Helixi142 – 1465Combined sources
Helixi147 – 17226Combined sources
Helixi173 – 1753Combined sources
Beta strandi177 – 1848Combined sources
Beta strandi186 – 1894Combined sources
Turni190 – 1945Combined sources
Helixi197 – 2059Combined sources
Helixi215 – 22612Combined sources
Helixi228 – 2303Combined sources
Beta strandi237 – 2415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4AFNX-ray2.30A/B/C/D1-247[»]
4AG3X-ray1.80A/B/C/D1-247[»]
4BNTX-ray2.30A/B/C/D1-247[»]
4BNUX-ray2.00A/B/C/D1-247[»]
4BNVX-ray2.50A/B/C/D1-247[»]
4BNWX-ray1.60A/B/C/D1-247[»]
4BNXX-ray2.30A/B/C/D1-247[»]
4BNYX-ray1.80A/B/C/D1-247[»]
4BNZX-ray2.50A/B/C/D1-247[»]
4BO0X-ray2.40A/B/C/D1-247[»]
4BO1X-ray2.20A/B/C/D1-247[»]
4BO2X-ray1.90A/B/C/D1-247[»]
4BO3X-ray2.50A/B/C/D1-247[»]
4BO4X-ray2.70A/B/C/D1-247[»]
4BO5X-ray2.60A/B/C/D1-247[»]
4BO6X-ray2.80A/B/C/D1-247[»]
4BO7X-ray2.60A/B/C/D1-247[»]
4BO8X-ray2.70A/B/C/D1-247[»]
4BO9X-ray2.90A/B/C/D1-247[»]
ProteinModelPortaliO54438.
SMRiO54438. Positions 1-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiO54438.
KOiK00059.
OMAiDEFGAID.
OrthoDBiEOG6N3CR8.
PhylomeDBiO54438.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54438-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLQGKVALV TGASRGIGQA IALELGRLGA VVIGTATSAS GAEKIAETLK
60 70 80 90 100
ANGVEGAGLV LDVSSDESVA ATLEHIQQHL GQPLIVVNNA GITRDNLLVR
110 120 130 140 150
MKDDEWFDVV NTNLNSLYRL SKAVLRGMTK ARWGRIINIG SVVGAMGNAG
160 170 180 190 200
QTNYAAAKAG LEGFTRALAR EVGSRAITVN AVAPGFIDTD MTRELPEAQR
210 220 230 240
EALLGQIPLG RLGQAEEIAK VVGFLASDGA AYVTGATVPV NGGMYMS
Length:247
Mass (Da):25,585
Last modified:June 1, 1998 - v1
Checksum:iAB83A2B95027445B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91631 Genomic DNA. Translation: AAB94395.1.
AE004091 Genomic DNA. Translation: AAG06355.1.
PIRiT12020.
RefSeqiNP_251657.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06355; AAG06355; PA2967.
GeneIDi880433.
KEGGipae:PA2967.
PATRICi19840487. VBIPseAer58763_3113.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91631 Genomic DNA. Translation: AAB94395.1 .
AE004091 Genomic DNA. Translation: AAG06355.1 .
PIRi T12020.
RefSeqi NP_251657.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4AFN X-ray 2.30 A/B/C/D 1-247 [» ]
4AG3 X-ray 1.80 A/B/C/D 1-247 [» ]
4BNT X-ray 2.30 A/B/C/D 1-247 [» ]
4BNU X-ray 2.00 A/B/C/D 1-247 [» ]
4BNV X-ray 2.50 A/B/C/D 1-247 [» ]
4BNW X-ray 1.60 A/B/C/D 1-247 [» ]
4BNX X-ray 2.30 A/B/C/D 1-247 [» ]
4BNY X-ray 1.80 A/B/C/D 1-247 [» ]
4BNZ X-ray 2.50 A/B/C/D 1-247 [» ]
4BO0 X-ray 2.40 A/B/C/D 1-247 [» ]
4BO1 X-ray 2.20 A/B/C/D 1-247 [» ]
4BO2 X-ray 1.90 A/B/C/D 1-247 [» ]
4BO3 X-ray 2.50 A/B/C/D 1-247 [» ]
4BO4 X-ray 2.70 A/B/C/D 1-247 [» ]
4BO5 X-ray 2.60 A/B/C/D 1-247 [» ]
4BO6 X-ray 2.80 A/B/C/D 1-247 [» ]
4BO7 X-ray 2.60 A/B/C/D 1-247 [» ]
4BO8 X-ray 2.70 A/B/C/D 1-247 [» ]
4BO9 X-ray 2.90 A/B/C/D 1-247 [» ]
ProteinModelPortali O54438.
SMRi O54438. Positions 1-247.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA2967.

Proteomic databases

PRIDEi O54438.

Protocols and materials databases

DNASUi 880433.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG06355 ; AAG06355 ; PA2967 .
GeneIDi 880433.
KEGGi pae:PA2967.
PATRICi 19840487. VBIPseAer58763_3113.

Organism-specific databases

PseudoCAPi PA2967.

Phylogenomic databases

eggNOGi COG1028.
InParanoidi O54438.
KOi K00059.
OMAi DEFGAID.
OrthoDBi EOG6N3CR8.
PhylomeDBi O54438.

Enzyme and pathway databases

UniPathwayi UPA00094 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsi TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (fabD)."
    Kutchma A.J., Hoang T.T., Schweizer H.P.
    J. Bacteriol. 181:5498-5504(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiFABG_PSEAE
AccessioniPrimary (citable) accession number: O54438
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3