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Protein

Urease subunit alpha

Gene

ureC

Organism
Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit gamma (ureA), Urease subunit alpha (ureC), Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi141Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi143Nickel 1; via tele nitrogenUniRule annotation1
Metal bindingi224Nickel 1; via carbamate groupUniRule annotation1
Metal bindingi224Nickel 2; via carbamate groupUniRule annotation1
Binding sitei226SubstrateUniRule annotation1
Metal bindingi253Nickel 2; via pros nitrogenUniRule annotation1
Metal bindingi279Nickel 2; via tele nitrogenUniRule annotation1
Active sitei327Proton donorUniRule annotation1
Metal bindingi367Nickel 1UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370

Protein family/group databases

MEROPSiM38.982

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
OrganismiActinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Taxonomic identifieri715 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000675351 – 572Urease subunit alphaAdd BLAST572

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PRIDEiO54420

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO54420
SMRiO54420
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 572UreaseUniRule annotationAdd BLAST437

Sequence similaritiesi

Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM Bacteria
COG0804 LUCA

Family and domain databases

CDDicd00375 Urease_alpha, 1 hit
Gene3Di2.30.40.10, 2 hits
HAMAPiMF_01953 Urease_alpha, 1 hit
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
IPR011612 Urease_alpha_N_dom
IPR017950 Urease_AS
IPR005848 Urease_asu
IPR017951 Urease_asu_c
IPR029754 Urease_Ni-bd
PANTHERiPTHR43440 PTHR43440, 1 hit
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
PF00449 Urease_alpha, 1 hit
PRINTSiPR01752 UREASE
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 2 hits
TIGRFAMsiTIGR01792 urease_alph, 1 hit
PROSITEiView protein in PROSITE
PS01120 UREASE_1, 1 hit
PS00145 UREASE_2, 1 hit
PS51368 UREASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

O54420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTIPRSQY VATYGPTVGD KVRLGDTDLW ATIEQDFLTK GDECKFGGGK
60 70 80 90 100
SVRDGMAQSS TATRDNPNVL DFALTNVMII DAKLGIIKAD IGIRDGRIVG
110 120 130 140 150
IGQAGNPDTM DNVTPNMIIG ASTEVHNGAH LIATAGGIDT HIHWICPQQA
160 170 180 190 200
QHAIENGITT MIGGGSGPAD GTHATTCTPG KFNIERMFQA CEALPVNIGF
210 220 230 240 250
FGKGNCSMLE PLKEQVVAGA LGLKIHEDWG ATPAVIDAAL KVADEMDVQV
260 270 280 290 300
AIHTDTLNES GFLEDTMKAI NGRVIHTFHT EGAGGGHAPD IIKAAMYPNV
310 320 330 340 350
LPASTNPNRP FTVNTIDEHL DMLMVCHHLD KRVPEDVAFA DSRIRPETIA
360 370 380 390 400
AEDILHDMGV FSIMSSDSQA MGRVGEVVTR TWQTADKMKA QRGALGDEGN
410 420 430 440 450
DNFRIKRYIA KYTINPAIAH GISQYVGSLE VGKLADIVLW KPQFFGVKPE
460 470 480 490 500
FVMKKGFISF AKMGDPNASI PTPQPVFYRP MFGANAKANT ESAVYFVSQA
510 520 530 540 550
SVDANIKAQY GIQKETLAVK GCRDVGKKDL VHNNATPEIT VDTERYEVRV
560 570
DGEHITCEPA TKVPLAQRYF LF
Length:572
Mass (Da):61,836
Last modified:June 1, 1998 - v1
Checksum:iBA1D793849277851
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89957 Genomic DNA Translation: AAC00060.1

Similar proteinsi

Entry informationi

Entry nameiURE1_ACTPL
AccessioniPrimary (citable) accession number: O54420
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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