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Protein

Urease subunit alpha

Gene

ureC

Organism
Actinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi141 – 1411Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi143 – 1431Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi224 – 2241Nickel 1; via carbamate groupUniRule annotation
Metal bindingi224 – 2241Nickel 2; via carbamate groupUniRule annotation
Binding sitei226 – 2261SubstrateUniRule annotation
Metal bindingi253 – 2531Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi279 – 2791Nickel 2; via tele nitrogenUniRule annotation
Active sitei327 – 3271Proton donorUniRule annotation
Metal bindingi367 – 3671Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
OrganismiActinobacillus pleuropneumoniae (Haemophilus pleuropneumoniae)
Taxonomic identifieri715 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Urease subunit alphaPRO_0000067535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei224 – 2241N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi228399.appser1_17370.

Structurei

3D structure databases

ProteinModelPortaliO54420.
SMRiO54420. Positions 5-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 572437UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALTIPRSQY VATYGPTVGD KVRLGDTDLW ATIEQDFLTK GDECKFGGGK
60 70 80 90 100
SVRDGMAQSS TATRDNPNVL DFALTNVMII DAKLGIIKAD IGIRDGRIVG
110 120 130 140 150
IGQAGNPDTM DNVTPNMIIG ASTEVHNGAH LIATAGGIDT HIHWICPQQA
160 170 180 190 200
QHAIENGITT MIGGGSGPAD GTHATTCTPG KFNIERMFQA CEALPVNIGF
210 220 230 240 250
FGKGNCSMLE PLKEQVVAGA LGLKIHEDWG ATPAVIDAAL KVADEMDVQV
260 270 280 290 300
AIHTDTLNES GFLEDTMKAI NGRVIHTFHT EGAGGGHAPD IIKAAMYPNV
310 320 330 340 350
LPASTNPNRP FTVNTIDEHL DMLMVCHHLD KRVPEDVAFA DSRIRPETIA
360 370 380 390 400
AEDILHDMGV FSIMSSDSQA MGRVGEVVTR TWQTADKMKA QRGALGDEGN
410 420 430 440 450
DNFRIKRYIA KYTINPAIAH GISQYVGSLE VGKLADIVLW KPQFFGVKPE
460 470 480 490 500
FVMKKGFISF AKMGDPNASI PTPQPVFYRP MFGANAKANT ESAVYFVSQA
510 520 530 540 550
SVDANIKAQY GIQKETLAVK GCRDVGKKDL VHNNATPEIT VDTERYEVRV
560 570
DGEHITCEPA TKVPLAQRYF LF
Length:572
Mass (Da):61,836
Last modified:June 1, 1998 - v1
Checksum:iBA1D793849277851
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89957 Genomic DNA. Translation: AAC00060.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89957 Genomic DNA. Translation: AAC00060.1.

3D structure databases

ProteinModelPortaliO54420.
SMRiO54420. Positions 5-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi228399.appser1_17370.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetic and biochemical analyses of Actinobacillus pleuropneumoniae urease."
    Bosse J.T., Macinnes J.I.
    Infect. Immun. 65:4389-4394(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CM5 / Serotype 1.

Entry informationi

Entry nameiURE1_ACTPL
AccessioniPrimary (citable) accession number: O54420
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.