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Protein

GTP pyrophosphokinase

Gene

relA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp (By similarity).By similarity

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway:ippGpp biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ppGpp from GTP.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GTP pyrophosphokinase YwaC (ywaC), GTP pyrophosphokinase (relA), GTP pyrophosphokinase YjbM (yjbM)
  2. no protein annotated in this organism
This subpathway is part of the pathway ppGpp biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ppGpp from GTP, the pathway ppGpp biosynthesis and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU27600-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
Ordered Locus Names:BSU27600
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU27600. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734GTP pyrophosphokinasePRO_0000166544Add
BLAST

Proteomic databases

PaxDbiO54408.

Interactioni

Protein-protein interaction databases

IntActiO54408. 1 interaction.
STRINGi224308.Bsubs1_010100015086.

Structurei

3D structure databases

ProteinModelPortaliO54408.
SMRiO54408. Positions 6-455, 656-734.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 149100HDAdd
BLAST
Domaini660 – 73475ACTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.Curated
Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
InParanoidiO54408.
KOiK00951.
OMAiGRPKHYY.
OrthoDBiEOG6SV551.
PhylomeDBiO54408.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANEQVLTAE QVIDKARSYL SDEHIAFVEK AYLYAEDAHR EQYRKSGEPY
60 70 80 90 100
IIHPIQVAGI LVDLEMDPST IAGGFLHDVV EDTDVTLDDL KEAFSEEVAM
110 120 130 140 150
LVDGVTKLGK IKYKSQEEQQ AENHRKMFVA MAQDIRVILI KLADRLHNMR
160 170 180 190 200
TLKHLPQEKQ RRISNETLEI FAPLAHRLGI SKIKWELEDT ALRYLNPQQY
210 220 230 240 250
YRIVNLMKKK RAERELYVDE VVNEVKKRVE EVNIKADFSG RPKHIYSIYR
260 270 280 290 300
KMVLQNKQFN EIYDLLAVRI LVNSIKDCYA VLGIIHTCWK PMPGRFKDYI
310 320 330 340 350
AMPKPNMYQS LHTTVIGPKG DPLEVQIRTF EMHEIAEYGV AAHWAYKEGK
360 370 380 390 400
AANEGATFEK KLSWFREILE FQNESTDAEE FMESLKIDLF SDMVYVFTPK
410 420 430 440 450
GDVIELPSGS VPIDFSYRIH SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE
460 470 480 490 500
ILTSKHSYGP SQDWVKLAQT SQAKHKIRQF FKKQRREENV EKGRELVEKE
510 520 530 540 550
IKNLDFELKD VLTPENIQKV ADKFNFSNEE DMYAAVGYNG ITALQVANRL
560 570 580 590 600
TEKERKQRDQ EEQEKIVQEV TGEPKPYPQG RKREAGVRVK GIDNLLVRLS
610 620 630 640 650
KCCNPVPGDD IVGFITKGRG VSVHREDCPN VKTNEAQERL IPVEWEHESQ
660 670 680 690 700
VQKRKEYNVE IEILGYDRRG LLNEVLQAVN ETKTNISSVS GKSDRNKVAT
710 720 730
IHMAIFIQNI NHLHKVVERI KQIRDIYSVR RVMN
Length:734
Mass (Da):84,824
Last modified:July 7, 2009 - v3
Checksum:i6F06A3E0B9FFAB36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86377 Genomic DNA. Translation: AAC46041.1.
AL009126 Genomic DNA. Translation: CAB14719.2.
PIRiC69691.
RefSeqiNP_390638.2. NC_000964.3.
WP_003229747.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14719; CAB14719; BSU27600.
GeneIDi936753.
KEGGibsu:BSU27600.
PATRICi18977384. VBIBacSub10457_2881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U86377 Genomic DNA. Translation: AAC46041.1.
AL009126 Genomic DNA. Translation: CAB14719.2.
PIRiC69691.
RefSeqiNP_390638.2. NC_000964.3.
WP_003229747.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliO54408.
SMRiO54408. Positions 6-455, 656-734.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO54408. 1 interaction.
STRINGi224308.Bsubs1_010100015086.

Proteomic databases

PaxDbiO54408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14719; CAB14719; BSU27600.
GeneIDi936753.
KEGGibsu:BSU27600.
PATRICi18977384. VBIBacSub10457_2881.

Organism-specific databases

GenoListiBSU27600. [Micado]

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
InParanoidiO54408.
KOiK00951.
OMAiGRPKHYY.
OrthoDBiEOG6SV551.
PhylomeDBiO54408.

Enzyme and pathway databases

UniPathwayiUPA00908; UER00884.
BioCyciBSUB:BSU27600-MONOMER.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a relA/spoT homologue from Bacillus subtilis."
    Wendrich T.M., Marahiel M.A.
    Mol. Microbiol. 26:65-79(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 320.

Entry informationi

Entry nameiRELA_BACSU
AccessioniPrimary (citable) accession number: O54408
Secondary accession number(s): O32043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 7, 2009
Last modified: July 22, 2015
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.