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O54408

- RELA_BACSU

UniProt

O54408 - RELA_BACSU

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Protein
GTP pyrophosphokinase
Gene
relA, BSU27600
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity.

Catalytic activityi

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. GTP diphosphokinase activity Source: UniProtKB-EC
  4. amino acid binding Source: InterPro
  5. kinase activity Source: UniProtKB-KW
  6. metal ion binding Source: InterPro
  7. phosphoric diester hydrolase activity Source: InterPro

GO - Biological processi

  1. guanosine tetraphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU27600-MONOMER.
UniPathwayiUPA00908; UER00884.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP pyrophosphokinase (EC:2.7.6.5)
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene namesi
Name:relA
Ordered Locus Names:BSU27600
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU27600. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734GTP pyrophosphokinase
PRO_0000166544Add
BLAST

Proteomic databases

PaxDbiO54408.

Interactioni

Protein-protein interaction databases

IntActiO54408. 1 interaction.
STRINGi224308.BSU27600.

Structurei

3D structure databases

ProteinModelPortaliO54408.
SMRiO54408. Positions 6-455, 656-734.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini50 – 149100HD
Add
BLAST
Domaini660 – 73475ACT
Add
BLAST

Sequence similaritiesi

Belongs to the RelA/SpoT family.
Contains 1 ACT domain.
Contains 1 HD domain.

Phylogenomic databases

eggNOGiCOG0317.
HOGENOMiHOG000018299.
KOiK00951.
OrthoDBiEOG6SV551.
PhylomeDBiO54408.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMiSSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54408-1 [UniParc]FASTAAdd to Basket

« Hide

MANEQVLTAE QVIDKARSYL SDEHIAFVEK AYLYAEDAHR EQYRKSGEPY    50
IIHPIQVAGI LVDLEMDPST IAGGFLHDVV EDTDVTLDDL KEAFSEEVAM 100
LVDGVTKLGK IKYKSQEEQQ AENHRKMFVA MAQDIRVILI KLADRLHNMR 150
TLKHLPQEKQ RRISNETLEI FAPLAHRLGI SKIKWELEDT ALRYLNPQQY 200
YRIVNLMKKK RAERELYVDE VVNEVKKRVE EVNIKADFSG RPKHIYSIYR 250
KMVLQNKQFN EIYDLLAVRI LVNSIKDCYA VLGIIHTCWK PMPGRFKDYI 300
AMPKPNMYQS LHTTVIGPKG DPLEVQIRTF EMHEIAEYGV AAHWAYKEGK 350
AANEGATFEK KLSWFREILE FQNESTDAEE FMESLKIDLF SDMVYVFTPK 400
GDVIELPSGS VPIDFSYRIH SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE 450
ILTSKHSYGP SQDWVKLAQT SQAKHKIRQF FKKQRREENV EKGRELVEKE 500
IKNLDFELKD VLTPENIQKV ADKFNFSNEE DMYAAVGYNG ITALQVANRL 550
TEKERKQRDQ EEQEKIVQEV TGEPKPYPQG RKREAGVRVK GIDNLLVRLS 600
KCCNPVPGDD IVGFITKGRG VSVHREDCPN VKTNEAQERL IPVEWEHESQ 650
VQKRKEYNVE IEILGYDRRG LLNEVLQAVN ETKTNISSVS GKSDRNKVAT 700
IHMAIFIQNI NHLHKVVERI KQIRDIYSVR RVMN 734
Length:734
Mass (Da):84,824
Last modified:July 7, 2009 - v3
Checksum:i6F06A3E0B9FFAB36
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86377 Genomic DNA. Translation: AAC46041.1.
AL009126 Genomic DNA. Translation: CAB14719.2.
PIRiC69691.
RefSeqiNP_390638.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB14719; CAB14719; BSU27600.
GeneIDi936753.
KEGGibsu:BSU27600.
PATRICi18977384. VBIBacSub10457_2881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U86377 Genomic DNA. Translation: AAC46041.1 .
AL009126 Genomic DNA. Translation: CAB14719.2 .
PIRi C69691.
RefSeqi NP_390638.2. NC_000964.3.

3D structure databases

ProteinModelPortali O54408.
SMRi O54408. Positions 6-455, 656-734.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O54408. 1 interaction.
STRINGi 224308.BSU27600.

Proteomic databases

PaxDbi O54408.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB14719 ; CAB14719 ; BSU27600 .
GeneIDi 936753.
KEGGi bsu:BSU27600.
PATRICi 18977384. VBIBacSub10457_2881.

Organism-specific databases

GenoListi BSU27600. [Micado ]

Phylogenomic databases

eggNOGi COG0317.
HOGENOMi HOG000018299.
KOi K00951.
OrthoDBi EOG6SV551.
PhylomeDBi O54408.

Enzyme and pathway databases

UniPathwayi UPA00908 ; UER00884 .
BioCyci BSUB:BSU27600-MONOMER.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view ]
SUPFAMi SSF81271. SSF81271. 1 hit.
TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a relA/spoT homologue from Bacillus subtilis."
    Wendrich T.M., Marahiel M.A.
    Mol. Microbiol. 26:65-79(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 320.

Entry informationi

Entry nameiRELA_BACSU
AccessioniPrimary (citable) accession number: O54408
Secondary accession number(s): O32043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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