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O54408 (RELA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
ppGpp synthase I
Gene names
Name:relA
Ordered Locus Names:BSU27600
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which is then hydrolyzed to form ppGpp By similarity.

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Pathway

Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step 1/2.

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 734734GTP pyrophosphokinase
PRO_0000166544

Regions

Domain50 – 149100HD
Domain660 – 73475ACT

Sequences

Sequence LengthMass (Da)Tools
O54408 [UniParc].

Last modified July 7, 2009. Version 3.
Checksum: 6F06A3E0B9FFAB36

FASTA73484,824
        10         20         30         40         50         60 
MANEQVLTAE QVIDKARSYL SDEHIAFVEK AYLYAEDAHR EQYRKSGEPY IIHPIQVAGI 

        70         80         90        100        110        120 
LVDLEMDPST IAGGFLHDVV EDTDVTLDDL KEAFSEEVAM LVDGVTKLGK IKYKSQEEQQ 

       130        140        150        160        170        180 
AENHRKMFVA MAQDIRVILI KLADRLHNMR TLKHLPQEKQ RRISNETLEI FAPLAHRLGI 

       190        200        210        220        230        240 
SKIKWELEDT ALRYLNPQQY YRIVNLMKKK RAERELYVDE VVNEVKKRVE EVNIKADFSG 

       250        260        270        280        290        300 
RPKHIYSIYR KMVLQNKQFN EIYDLLAVRI LVNSIKDCYA VLGIIHTCWK PMPGRFKDYI 

       310        320        330        340        350        360 
AMPKPNMYQS LHTTVIGPKG DPLEVQIRTF EMHEIAEYGV AAHWAYKEGK AANEGATFEK 

       370        380        390        400        410        420 
KLSWFREILE FQNESTDAEE FMESLKIDLF SDMVYVFTPK GDVIELPSGS VPIDFSYRIH 

       430        440        450        460        470        480 
SEIGNKTIGA KVNGKMVTLD HKLRTGDIVE ILTSKHSYGP SQDWVKLAQT SQAKHKIRQF 

       490        500        510        520        530        540 
FKKQRREENV EKGRELVEKE IKNLDFELKD VLTPENIQKV ADKFNFSNEE DMYAAVGYNG 

       550        560        570        580        590        600 
ITALQVANRL TEKERKQRDQ EEQEKIVQEV TGEPKPYPQG RKREAGVRVK GIDNLLVRLS 

       610        620        630        640        650        660 
KCCNPVPGDD IVGFITKGRG VSVHREDCPN VKTNEAQERL IPVEWEHESQ VQKRKEYNVE 

       670        680        690        700        710        720 
IEILGYDRRG LLNEVLQAVN ETKTNISSVS GKSDRNKVAT IHMAIFIQNI NHLHKVVERI 

       730 
KQIRDIYSVR RVMN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a relA/spoT homologue from Bacillus subtilis."
Wendrich T.M., Marahiel M.A.
Mol. Microbiol. 26:65-79(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 320.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U86377 Genomic DNA. Translation: AAC46041.1.
AL009126 Genomic DNA. Translation: CAB14719.2.
PIRC69691.
RefSeqNP_390638.2. NC_000964.3.

3D structure databases

ProteinModelPortalO54408.
SMRO54408. Positions 6-455, 656-734.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO54408. 1 interaction.
STRING224308.BSU27600.

Proteomic databases

PaxDbO54408.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB14719; CAB14719; BSU27600.
GeneID936753.
KEGGbsu:BSU27600.
PATRIC18977384. VBIBacSub10457_2881.

Organism-specific databases

GenoListBSU27600. [Micado]

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018299.
KOK00951.
OrthoDBEOG6SV551.
PhylomeDBO54408.

Enzyme and pathway databases

BioCycBSUB:BSU27600-MONOMER.
UniPathwayUPA00908; UER00884.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PfamPF01842. ACT. 1 hit.
PF01966. HD. 1 hit.
PF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRELA_BACSU
AccessionPrimary (citable) accession number: O54408
Secondary accession number(s): O32043
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 7, 2009
Last modified: July 9, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList