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O54315

- BGAL2_THESP

UniProt

O54315 - BGAL2_THESP

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Protein
Beta-galactosidase BgaA
Gene
bgaA
Organism
Thermus sp.
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), o-nitrophenyl-beta-D-galactopyranoside (ONPG) and lactose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Kineticsi

  1. KM=1.6 mM for ONPG (at 70 degrees Celsius and pH 7.0)1 Publication

Vmax=191000 nmol/min/mg enzyme with ONPG as substrate (at 70 degrees Celsius and pH 7.0)

Vmax=13000 nmol/min/mg enzyme with lactose as substrate (at 70 degrees Celsius and pH 7.0)

Vmax=435000 nmol/min/mg enzyme with ONPG as substrate (at 70 degrees Celsius and pH 5.0)

pH dependencei

Optimum pH is 5.0 with ONPG as substrate.

Temperature dependencei

Optimum temperature is 70 degrees Celsius with ONPG as substrate. Stable for several months at 4 or -20 degrees Celsius in the presence of 10% glycerol. Activity increases continuously from 30 to 90 degrees Celsius and drops dramatically at higher temperatures. Retains 50% of its initial activity after 1 hour incubation at 70 degrees Celsius. Retains 90% and 50% of activity after 30 minutes of incubation at 70 and 80 degrees Celsius, respectively. The residual activity of the enzyme decreases to an undetectable level after heating for 30 minutes at 90 degrees Celsius. The enzyme still maintains significant activity at 30 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021Substrate By similarity
Metal bindingi106 – 1061Zinc By similarity
Binding sitei140 – 1401Substrate By similarity
Active sitei141 – 1411Proton donor By similarity
Metal bindingi150 – 1501Zinc By similarity
Metal bindingi152 – 1521Zinc By similarity
Metal bindingi155 – 1551Zinc By similarity
Active sitei312 – 3121Nucleophile By similarity
Binding sitei320 – 3201Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaA (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:bgaA
OrganismiThermus sp.
Taxonomic identifieri275 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Beta-galactosidase BgaA
PRO_0000407696Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO54315.
SMRiO54315. Positions 1-644.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 3634Substrate binding

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

O54315-1 [UniParc]FASTAAdd to Basket

« Hide

MLGVCYYPEH WPEERWEEDF KAMRALGLRY VRLGEFAWSA LEPTPGALRW    50
GWLDRVLDLA QKEGLAVVLG TPTATPPKWL VDRYPEILPV DREGRRRNFG 100
GRRHYCFSSP AYREETARIV ALLAERYGRH PAVVGFQVDN EFGCHGTVRC 150
YCPNCREAFR GWLRAKYGTI DALNAAWGTV FWSQTYRDFG EVELPHLTVA 200
EANPSHLLDY YRFASDQVRA YNRFQVDLLR DNAPGRFITH NFMGFFTDLD 250
PFALAEDLDF AAWDSYPLGF TDLMPLPQEE KVQWARTGHP DVAAFHHDLY 300
RGVGRGRFWV MEQQPGPVNW APHNPSPAPG MVRLWTWEAI AHGAEVVSYF 350
RWRQAPFAQE QMQAGFNRPD FQPEVAFFEV QRVAEELSAL PLPPAGCAPV 400
ALVYDSEAAW VFEIQPQGAE WKYLTLVFSF YSVFRRLGLE VDIFKPGAEL 450
GGYGLVVVPS LPIVRKEALE ALSQADGLVI VGPRSGSKTE KFQIPPEIPP 500
GALQALLPLK VVRVESLPPG LLEEAEGPWG RFAFGVWREW VETDLPPLLR 550
FTDGGGILFR RGRYLYLAAW PSPELLFALC QSLAEEAGLH PRFLPEGLRL 600
RRRGPLVFAF NYGPEVVEAP APPGVRFLLG DRRIPPHDLA VWEET 645
Length:645
Mass (Da):73,221
Last modified:June 1, 1998 - v1
Checksum:i7A2AC9EAB6BAC944
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 32LG → ST AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z93773 Genomic DNA. Translation: CAB07810.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z93773 Genomic DNA. Translation: CAB07810.1 .

3D structure databases

ProteinModelPortali O54315.
SMRi O54315. Positions 1-644.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Structure of the beta-galactosidase gene from Thermus sp. strain T2: expression in Escherichia coli and purification in a single step of an active fusion protein."
    Vian A., Carrascosa A.V., Garcia J.L., Cortes E.
    Appl. Environ. Microbiol. 64:2187-2191(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 27737 / T2.

Entry informationi

Entry nameiBGAL2_THESP
AccessioniPrimary (citable) accession number: O54315
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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