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O54315

- BGAL2_THESP

UniProt

O54315 - BGAL2_THESP

Protein

Beta-galactosidase BgaA

Gene

bgaA

Organism
Thermus sp.
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Hydrolyzes chromogen 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal), o-nitrophenyl-beta-D-galactopyranoside (ONPG) and lactose.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

    Kineticsi

    1. KM=1.6 mM for ONPG (at 70 degrees Celsius and pH 7.0)1 Publication

    Vmax=191000 nmol/min/mg enzyme with ONPG as substrate (at 70 degrees Celsius and pH 7.0)1 Publication

    Vmax=13000 nmol/min/mg enzyme with lactose as substrate (at 70 degrees Celsius and pH 7.0)1 Publication

    Vmax=435000 nmol/min/mg enzyme with ONPG as substrate (at 70 degrees Celsius and pH 5.0)1 Publication

    pH dependencei

    Optimum pH is 5.0 with ONPG as substrate.1 Publication

    Temperature dependencei

    Optimum temperature is 70 degrees Celsius with ONPG as substrate. Stable for several months at 4 or -20 degrees Celsius in the presence of 10% glycerol. Activity increases continuously from 30 to 90 degrees Celsius and drops dramatically at higher temperatures. Retains 50% of its initial activity after 1 hour incubation at 70 degrees Celsius. Retains 90% and 50% of activity after 30 minutes of incubation at 70 and 80 degrees Celsius, respectively. The residual activity of the enzyme decreases to an undetectable level after heating for 30 minutes at 90 degrees Celsius. The enzyme still maintains significant activity at 30 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei102 – 1021SubstrateBy similarity
    Metal bindingi106 – 1061ZincBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Active sitei141 – 1411Proton donorBy similarity
    Metal bindingi150 – 1501ZincBy similarity
    Metal bindingi152 – 1521ZincBy similarity
    Metal bindingi155 – 1551ZincBy similarity
    Active sitei312 – 3121NucleophileBy similarity
    Binding sitei320 – 3201SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. galactose metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    CAZyiGH42. Glycoside Hydrolase Family 42.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase BgaA (EC:3.2.1.23)
    Short name:
    Beta-galBy similarity
    Gene namesi
    Name:bgaA1 Publication
    OrganismiThermus sp.
    Taxonomic identifieri275 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 645645Beta-galactosidase BgaAPRO_0000407696Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliO54315.
    SMRiO54315. Positions 1-644.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni360 – 3634Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 42 family.Sequence Analysis

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProiIPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR013780. Glyco_hydro_13_b.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001084. B-galactosidase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O54315-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLGVCYYPEH WPEERWEEDF KAMRALGLRY VRLGEFAWSA LEPTPGALRW    50
    GWLDRVLDLA QKEGLAVVLG TPTATPPKWL VDRYPEILPV DREGRRRNFG 100
    GRRHYCFSSP AYREETARIV ALLAERYGRH PAVVGFQVDN EFGCHGTVRC 150
    YCPNCREAFR GWLRAKYGTI DALNAAWGTV FWSQTYRDFG EVELPHLTVA 200
    EANPSHLLDY YRFASDQVRA YNRFQVDLLR DNAPGRFITH NFMGFFTDLD 250
    PFALAEDLDF AAWDSYPLGF TDLMPLPQEE KVQWARTGHP DVAAFHHDLY 300
    RGVGRGRFWV MEQQPGPVNW APHNPSPAPG MVRLWTWEAI AHGAEVVSYF 350
    RWRQAPFAQE QMQAGFNRPD FQPEVAFFEV QRVAEELSAL PLPPAGCAPV 400
    ALVYDSEAAW VFEIQPQGAE WKYLTLVFSF YSVFRRLGLE VDIFKPGAEL 450
    GGYGLVVVPS LPIVRKEALE ALSQADGLVI VGPRSGSKTE KFQIPPEIPP 500
    GALQALLPLK VVRVESLPPG LLEEAEGPWG RFAFGVWREW VETDLPPLLR 550
    FTDGGGILFR RGRYLYLAAW PSPELLFALC QSLAEEAGLH PRFLPEGLRL 600
    RRRGPLVFAF NYGPEVVEAP APPGVRFLLG DRRIPPHDLA VWEET 645
    Length:645
    Mass (Da):73,221
    Last modified:June 1, 1998 - v1
    Checksum:i7A2AC9EAB6BAC944
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 32LG → ST AA sequence (PubMed:9603833)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z93773 Genomic DNA. Translation: CAB07810.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z93773 Genomic DNA. Translation: CAB07810.1 .

    3D structure databases

    ProteinModelPortali O54315.
    SMRi O54315. Positions 1-644.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH42. Glycoside Hydrolase Family 42.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    3.40.50.880. 1 hit.
    InterProi IPR013739. Beta_galactosidase_C.
    IPR013738. Beta_galactosidase_Trimer.
    IPR029062. Class_I_gatase-like.
    IPR013780. Glyco_hydro_13_b.
    IPR003476. Glyco_hydro_42.
    IPR013529. Glyco_hydro_42_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02449. Glyco_hydro_42. 1 hit.
    PF08533. Glyco_hydro_42C. 1 hit.
    PF08532. Glyco_hydro_42M. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001084. B-galactosidase. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF52317. SSF52317. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the beta-galactosidase gene from Thermus sp. strain T2: expression in Escherichia coli and purification in a single step of an active fusion protein."
      Vian A., Carrascosa A.V., Garcia J.L., Cortes E.
      Appl. Environ. Microbiol. 64:2187-2191(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: ATCC 27737 / T2Imported.

    Entry informationi

    Entry nameiBGAL2_THESP
    AccessioniPrimary (citable) accession number: O54315
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 3, 2011
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3