ID   METF_STRLI              Reviewed;         307 AA.
AC   O54235;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   16-JUN-2009, entry version 40.
DE   RecName: Full=5,10-methylenetetrahydrofolate reductase;
DE            EC=1.5.1.20;
GN   Name=metF;
OS   Streptomyces lividans.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=1916;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=66 / 1326;
RX   MEDLINE=98175688; PubMed=9515933;
RA   Blanco J., Coque J.R., Martin J.;
RT   "The folate branch of the methionine biosynthesis pathway in
RT   Streptomyces lividans: disruption of the 5,10-
RT   methylenetetrahydrofolate reductase gene leads to methionine
RT   auxotrophy.";
RL   J. Bacteriol. 180:1586-1591(1998).
CC   -!- CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)(+) = 5,10-
CC       methylenetetrahydrofolate + NAD(P)H.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate pathway.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase
CC       family.
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DR   EMBL; AJ001630; CAA04885.1; -; Genomic_DNA.
DR   HSSP; P00394; 1B5T.
DR   BRENDA; 1.5.1.20; 15238.
DR   GO; GO:0004489; F:methylenetetrahydrofolate reductase (NADPH)...; IEA:EC.
DR   GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR003171; Mehydrof_redctse.
DR   InterPro; IPR004620; MTHF_reductase_bac.
DR   Pfam; PF02219; MTHFR; 1.
DR   TIGRFAMs; TIGR00676; fadh2; 1.
PE   3: Inferred from homology;
KW   Amino-acid biosynthesis; FAD; Flavoprotein; Methionine biosynthesis;
KW   NAD; NADP; Oxidoreductase.
FT   CHAIN         1    307       5,10-methylenetetrahydrofolate reductase.
FT                                /FTId=PRO_0000190266.
SQ   SEQUENCE   307 AA;  33268 MW;  0CA09C336036D8A9 CRC64;
     MALGTASTRT DRARTVRDIL ATGKTTYSFE FSAPKTPKGE KNLWSALRRV EAVAPDFVSV
     TYGAGGSTRA GTVRETQQIV ADTTLTPVAH LTAVDHSVAE LRNIIGQYAD AGIRNMLAVR
     GDPPGDPNAD WIAHPEGLTY AAELVRLIKE SGDFCVGVAA FPEMHPRSAD WDTDVTNFVD
     KCRAGADYAI TQMFFQPDSY LRLRDRVAAA GCATPVIPEV MPVTSVKMLE RLPKLSNASF
     PAELKERILT AKDDPAAVRS IGIEFATEFC ARLLAEGVPG LHFITLNNST ATLEIYENLG
     LHHPPRA
//