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Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei30Proton donor/acceptorBy similarity1
Binding sitei90FADBy similarity1
Binding sitei122SubstrateBy similarity1
Binding sitei192SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 35NADBy similarity6
Nucleotide bindingi61 – 64FADBy similarity4
Nucleotide bindingi61 – 62NADBy similarity2
Nucleotide bindingi120 – 122FADBy similarity3
Nucleotide bindingi140 – 141FADBy similarity2
Nucleotide bindingi165 – 168FADBy similarity4
Nucleotide bindingi174 – 181FADBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902661 – 3075,10-methylenetetrahydrofolate reductaseAdd BLAST307

Proteomic databases

PRIDEiO54235

Structurei

3D structure databases

ProteinModelPortaliO54235
SMRiO54235
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

CDDicd00537 MTHFR, 1 hit
InterProiView protein in InterPro
IPR029041 FAD-linked_oxidoreductase-like
IPR003171 Mehydrof_redctse
IPR004620 MTHF_reductase_bac
PfamiView protein in Pfam
PF02219 MTHFR, 1 hit
SUPFAMiSSF51730 SSF51730, 1 hit
TIGRFAMsiTIGR00676 fadh2, 1 hit

Sequencei

Sequence statusi: Complete.

O54235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGTASTRT DRARTVRDIL ATGKTTYSFE FSAPKTPKGE KNLWSALRRV
60 70 80 90 100
EAVAPDFVSV TYGAGGSTRA GTVRETQQIV ADTTLTPVAH LTAVDHSVAE
110 120 130 140 150
LRNIIGQYAD AGIRNMLAVR GDPPGDPNAD WIAHPEGLTY AAELVRLIKE
160 170 180 190 200
SGDFCVGVAA FPEMHPRSAD WDTDVTNFVD KCRAGADYAI TQMFFQPDSY
210 220 230 240 250
LRLRDRVAAA GCATPVIPEV MPVTSVKMLE RLPKLSNASF PAELKERILT
260 270 280 290 300
AKDDPAAVRS IGIEFATEFC ARLLAEGVPG LHFITLNNST ATLEIYENLG

LHHPPRA
Length:307
Mass (Da):33,268
Last modified:June 1, 1998 - v1
Checksum:i0CA09C336036D8A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001630 Genomic DNA Translation: CAA04885.1

Similar proteinsi

Entry informationi

Entry nameiMETF_STRLI
AccessioniPrimary (citable) accession number: O54235
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

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