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O54235

- METF_STRLI

UniProt

O54235 - METF_STRLI

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Protein

5,10-methylenetetrahydrofolate reductase

Gene
metF
Organism
Streptomyces lividans
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei30 – 301Proton donor/acceptor By similarity
Binding sitei90 – 901FAD By similarity
Binding sitei122 – 1221Substrate By similarity
Binding sitei192 – 1921Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 356NAD By similarity
Nucleotide bindingi61 – 644FAD By similarity
Nucleotide bindingi61 – 622NAD By similarity
Nucleotide bindingi120 – 1223FAD By similarity
Nucleotide bindingi140 – 1412FAD By similarity
Nucleotide bindingi165 – 1684FAD By similarity
Nucleotide bindingi174 – 1818FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3073075,10-methylenetetrahydrofolate reductasePRO_0000190266Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO54235.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

O54235-1 [UniParc]FASTAAdd to Basket

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MALGTASTRT DRARTVRDIL ATGKTTYSFE FSAPKTPKGE KNLWSALRRV    50
EAVAPDFVSV TYGAGGSTRA GTVRETQQIV ADTTLTPVAH LTAVDHSVAE 100
LRNIIGQYAD AGIRNMLAVR GDPPGDPNAD WIAHPEGLTY AAELVRLIKE 150
SGDFCVGVAA FPEMHPRSAD WDTDVTNFVD KCRAGADYAI TQMFFQPDSY 200
LRLRDRVAAA GCATPVIPEV MPVTSVKMLE RLPKLSNASF PAELKERILT 250
AKDDPAAVRS IGIEFATEFC ARLLAEGVPG LHFITLNNST ATLEIYENLG 300
LHHPPRA 307
Length:307
Mass (Da):33,268
Last modified:June 1, 1998 - v1
Checksum:i0CA09C336036D8A9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001630 Genomic DNA. Translation: CAA04885.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ001630 Genomic DNA. Translation: CAA04885.1 .

3D structure databases

ProteinModelPortali O54235.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The folate branch of the methionine biosynthesis pathway in Streptomyces lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene leads to methionine auxotrophy."
    Blanco J., Coque J.R., Martin J.
    J. Bacteriol. 180:1586-1591(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 66 / 1326.

Entry informationi

Entry nameiMETF_STRLI
AccessioniPrimary (citable) accession number: O54235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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