Reviewed, UniProtKB/Swiss-Prot O54235 (METF_STRLI)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:

metF

Organism

Streptomyces lividans

Taxonomic identifier

1916 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	5,10-methylenetetrahydrofolate reductase		PRO_0000190266

Sequences

	Sequence		Length	Mass (Da)
	O54235-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0CA09C336036D8A9	FASTA	307	33,268
	10 20 30 40 50 60 MALGTASTRT DRARTVRDIL ATGKTTYSFE FSAPKTPKGE KNLWSALRRV EAVAPDFVSV 70 80 90 100 110 120 TYGAGGSTRA GTVRETQQIV ADTTLTPVAH LTAVDHSVAE LRNIIGQYAD AGIRNMLAVR 130 140 150 160 170 180 GDPPGDPNAD WIAHPEGLTY AAELVRLIKE SGDFCVGVAA FPEMHPRSAD WDTDVTNFVD 190 200 210 220 230 240 KCRAGADYAI TQMFFQPDSY LRLRDRVAAA GCATPVIPEV MPVTSVKMLE RLPKLSNASF 250 260 270 280 290 300 PAELKERILT AKDDPAAVRS IGIEFATEFC ARLLAEGVPG LHFITLNNST ATLEIYENLG LHHPPRA

References

[1]

"The folate branch of the methionine biosynthesis pathway in Streptomyces lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene leads to methionine auxotrophy." Blanco J., Coque J.R., Martin J. J. Bacteriol. 180:1586-1591(1998) [PubMed: 9515933] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 66 / 1326.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ001630 Genomic DNA. Translation: CAA04885.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.20. 15238.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_STRLI

Accession

Primary (citable) accession number: O54235

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)