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O54235 (METF_STRLI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:metF
OrganismStreptomyces lividans
Taxonomic identifier1916 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Methionine biosynthesis
   LigandFAD
Flavoprotein
NAD
NADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processmethionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmethylenetetrahydrofolate reductase (NAD(P)H) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3073075,10-methylenetetrahydrofolate reductase
PRO_0000190266

Regions

Nucleotide binding30 – 356NAD By similarity
Nucleotide binding61 – 644FAD By similarity
Nucleotide binding61 – 622NAD By similarity
Nucleotide binding120 – 1223FAD By similarity
Nucleotide binding140 – 1412FAD By similarity
Nucleotide binding165 – 1684FAD By similarity
Nucleotide binding174 – 1818FAD By similarity

Sites

Active site301Proton donor/acceptor By similarity
Binding site901FAD By similarity
Binding site1221Substrate By similarity
Binding site1921Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
O54235 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 0CA09C336036D8A9

FASTA30733,268
        10         20         30         40         50         60 
MALGTASTRT DRARTVRDIL ATGKTTYSFE FSAPKTPKGE KNLWSALRRV EAVAPDFVSV 

        70         80         90        100        110        120 
TYGAGGSTRA GTVRETQQIV ADTTLTPVAH LTAVDHSVAE LRNIIGQYAD AGIRNMLAVR 

       130        140        150        160        170        180 
GDPPGDPNAD WIAHPEGLTY AAELVRLIKE SGDFCVGVAA FPEMHPRSAD WDTDVTNFVD 

       190        200        210        220        230        240 
KCRAGADYAI TQMFFQPDSY LRLRDRVAAA GCATPVIPEV MPVTSVKMLE RLPKLSNASF 

       250        260        270        280        290        300 
PAELKERILT AKDDPAAVRS IGIEFATEFC ARLLAEGVPG LHFITLNNST ATLEIYENLG 


LHHPPRA 

« Hide

References

[1]"The folate branch of the methionine biosynthesis pathway in Streptomyces lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene leads to methionine auxotrophy."
Blanco J., Coque J.R., Martin J.
J. Bacteriol. 180:1586-1591(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 66 / 1326.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001630 Genomic DNA. Translation: CAA04885.1.

3D structure databases

ProteinModelPortalO54235.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

InterProIPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00676. fadh2. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETF_STRLI
AccessionPrimary (citable) accession number: O54235
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: December 11, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways