O54196 (NRDJ_STRCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vitamin B12-dependent ribonucleotide reductase
EC=1.17.4.1

Alternative name(s):
Ribonucleoside-diphosphate reductase nrdJ

Gene names

Name:

nrdJ

Organism

Streptomyces clavuligerus

Taxonomic identifier

1901 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	961 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) Probable.
Subunit structure	Homotetramer.
Developmental stage	Expressed during the exponential phase of growth.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 961	961	Vitamin B12-dependent ribonucleotide reductase		PRO_0000229028
Sites
	Active site	160	1	By similarity
	Active site	366	1	By similarity
	Active site	377	1	By similarity
	Active site	954	1	By similarity
	Active site	960	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O54196 [UniParc]. Last modified November 1, 1998. Version 2. Checksum: 9C9D30730047A9F7	FASTA	961	104,791
	10 20 30 40 50 60 MTETTSGPAR GSRTKGTKAT KGLRIERVHT TPGVHPYDEV VWERRDVVMT NWRDGSVNFE 70 80 90 100 110 120 QRGVEFPDFW SVNAVNIVTS KYFRGAVGTP QRETGLKQLI DRIVKTYRKA GEEYKYFASP 130 140 150 160 170 180 ADAEIFEHEL AYALLHQIFS FNSPVWFNVG TPQPQQVSAC FILSVDDSME SILDWYKEEG 190 200 210 220 230 240 MIFKGGSGAG LNLSRIRSSK ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI 250 260 270 280 290 300 LDVDHPDIEG FIETKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDEFMRA 310 320 330 340 350 360 VESGSAFGLR ARMTGEIIEQ VDAKALFRKM AQAAWACADP GIQYDDTINR WHTCPESGRI 370 380 390 400 410 420 NGSNPCSEYM HLDNTSCNLA SLNLMKFLTD DGEGNQSFDV ERFAKVVELV ITAMDISICF 430 440 450 460 470 480 ADFPTQKIGE NTRAFRQLGI GYANLGALLM ATGHAYDSDG GRAIAGAISS LMTGTSYRRS 490 500 510 520 530 540 AELAAVVGPY DGYARNAAPH NQVMRQHADA NDTAVRMDDL DTPIWAAATE TWQDVLRLGE 550 560 570 580 590 600 KNGFRNAQAS VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR 610 620 630 640 650 660 LGYQAEQIEA IVEHIAEHGN VLDAPGLKTE HYKVFDCAMG ERSISAMGHV RMMAAIQPWI 670 680 690 700 710 720 SGALSKTVNM PESATVEEVE EIYFEAWKMG VKALAIYRDN CKVGQPLSAK TKEKEQDGIA 730 740 750 760 770 780 EKTEDTIRAA VEKVIEYRPV RKRLPKGRPG ITTSFTVGGA EGYMTANSYP DDGLGEVFLK 790 800 810 820 830 840 MSKQGSTLAG MMDAFSIAVS VGLQYGVPLE TYVSKFTNMR FEPAGMTDDP DVRMAQSIVD 850 860 870 880 890 900 NIFRRLALDF LPFETRSALG IHSAEERQRH LDTGSYEQVI EEDELDVEGL AQSAPRQQIP 910 920 930 940 950 960 AVPAAPAEIP APKQAHTSAE LVEMQLGISA DAPLCFSCGT KMQRAGSCYI CEGCGSTSGC S

References

[1]

"Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth." Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y. Microbiology 148:391-404(2002) [PubMed: 11832503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224870 Genomic DNA. Translation: CAA12167.1.
3D structure databases
ProteinModelPortal	O54196.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013344. RDP_Rdtase_AdoCbl-dep. IPR000788. Ribncl_red_lg_C. IPR013678. Ribonuc_red_2_N. IPR024434. TSCPD_dom. [Graphical view]
Pfam	PF08471. Ribonuc_red_2_N. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF12637. TSCPD. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02504. NrdJ_Z. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NRDJ_STRCL

Accession

Primary (citable) accession number: O54196

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 21, 2006
Last sequence update:	November 1, 1998
Last modified:	October 19, 2011
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program