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O54196

- NRDJ_STRC2

UniProt

O54196 - NRDJ_STRC2

Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (01 Nov 1998)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

    Cofactori

    5'-deoxyadenosylcobalamine (coenzyme B12).Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei143 – 1431SubstrateBy similarity
    Binding sitei188 – 1881Substrate; via amide nitrogenBy similarity
    Active sitei364 – 3641Proton acceptorBy similarity
    Active sitei366 – 3661Cysteine radical intermediateBy similarity
    Active sitei368 – 3681Proton acceptorBy similarity

    GO - Molecular functioni

    1. cobalamin binding Source: UniProtKB-KW
    2. cobalt ion binding Source: InterPro
    3. nucleotide binding Source: UniProtKB-KW
    4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    Cobalamin, Cobalt, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleoside-diphosphate reductase NrdJ
    Gene namesi
    Name:nrdJ
    OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
    Taxonomic identifieri443255 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 961961Vitamin B12-dependent ribonucleotide reductasePRO_0000229028Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi160 ↔ 377Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Developmental stagei

    Expressed during the exponential phase of growth.

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    3D structure databases

    ProteinModelPortaliO54196.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni159 – 1602Substrate bindingBy similarity
    Regioni364 – 3685Substrate bindingBy similarity
    Regioni554 – 5585Substrate bindingBy similarity

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    [Graphical view]
    PfamiPF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF12637. TSCPD. 1 hit.
    [Graphical view]
    PRINTSiPR01183. RIBORDTASEM1.
    TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O54196-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTETTSGPAR GSRTKGTKAT KGLRIERVHT TPGVHPYDEV VWERRDVVMT    50
    NWRDGSVNFE QRGVEFPDFW SVNAVNIVTS KYFRGAVGTP QRETGLKQLI 100
    DRIVKTYRKA GEEYKYFASP ADAEIFEHEL AYALLHQIFS FNSPVWFNVG 150
    TPQPQQVSAC FILSVDDSME SILDWYKEEG MIFKGGSGAG LNLSRIRSSK 200
    ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI LDVDHPDIEG 250
    FIETKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDEFMRA 300
    VESGSAFGLR ARMTGEIIEQ VDAKALFRKM AQAAWACADP GIQYDDTINR 350
    WHTCPESGRI NGSNPCSEYM HLDNTSCNLA SLNLMKFLTD DGEGNQSFDV 400
    ERFAKVVELV ITAMDISICF ADFPTQKIGE NTRAFRQLGI GYANLGALLM 450
    ATGHAYDSDG GRAIAGAISS LMTGTSYRRS AELAAVVGPY DGYARNAAPH 500
    NQVMRQHADA NDTAVRMDDL DTPIWAAATE TWQDVLRLGE KNGFRNAQAS 550
    VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR 600
    LGYQAEQIEA IVEHIAEHGN VLDAPGLKTE HYKVFDCAMG ERSISAMGHV 650
    RMMAAIQPWI SGALSKTVNM PESATVEEVE EIYFEAWKMG VKALAIYRDN 700
    CKVGQPLSAK TKEKEQDGIA EKTEDTIRAA VEKVIEYRPV RKRLPKGRPG 750
    ITTSFTVGGA EGYMTANSYP DDGLGEVFLK MSKQGSTLAG MMDAFSIAVS 800
    VGLQYGVPLE TYVSKFTNMR FEPAGMTDDP DVRMAQSIVD NIFRRLALDF 850
    LPFETRSALG IHSAEERQRH LDTGSYEQVI EEDELDVEGL AQSAPRQQIP 900
    AVPAAPAEIP APKQAHTSAE LVEMQLGISA DAPLCFSCGT KMQRAGSCYI 950
    CEGCGSTSGC S 961
    Length:961
    Mass (Da):104,791
    Last modified:November 1, 1998 - v2
    Checksum:i9C9D30730047A9F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ224870 Genomic DNA. Translation: CAA12167.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ224870 Genomic DNA. Translation: CAA12167.1 .

    3D structure databases

    ProteinModelPortali O54196.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR013678. RNR_2_N.
    IPR000788. RNR_lg_C.
    IPR013344. RNR_NrdJ/NrdZ.
    IPR024434. TSCPD_dom.
    [Graphical view ]
    Pfami PF08471. Ribonuc_red_2_N. 1 hit.
    PF02867. Ribonuc_red_lgC. 1 hit.
    PF12637. TSCPD. 1 hit.
    [Graphical view ]
    PRINTSi PR01183. RIBORDTASEM1.
    TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
      Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y.
      Microbiology 148:391-404(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

    Entry informationi

    Entry nameiNRDJ_STRC2
    AccessioniPrimary (citable) accession number: O54196
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: November 1, 1998
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3