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O54196 (NRDJ_STRC2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Vitamin B12-dependent ribonucleotide reductase

EC=1.17.4.1
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene names
Name:nrdJ
OrganismStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifier443255 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length961 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

Catalytic activity

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactor

5'-deoxyadenosylcobalamine (coenzyme B12) Probable.

Subunit structure

Homotetramer.

Developmental stage

Expressed during the exponential phase of growth.

Sequence similarities

Belongs to the ribonucleoside diphosphate reductase class-2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 961961Vitamin B12-dependent ribonucleotide reductase
PRO_0000229028

Regions

Region159 – 1602Substrate binding By similarity
Region364 – 3685Substrate binding By similarity
Region554 – 5585Substrate binding By similarity

Sites

Active site3641Proton acceptor By similarity
Active site3661Cysteine radical intermediate By similarity
Active site3681Proton acceptor By similarity
Binding site1431Substrate By similarity
Binding site1881Substrate; via amide nitrogen By similarity

Amino acid modifications

Disulfide bond160 ↔ 377Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
O54196 [UniParc].

Last modified November 1, 1998. Version 2.
Checksum: 9C9D30730047A9F7

FASTA961104,791
        10         20         30         40         50         60 
MTETTSGPAR GSRTKGTKAT KGLRIERVHT TPGVHPYDEV VWERRDVVMT NWRDGSVNFE 

        70         80         90        100        110        120 
QRGVEFPDFW SVNAVNIVTS KYFRGAVGTP QRETGLKQLI DRIVKTYRKA GEEYKYFASP 

       130        140        150        160        170        180 
ADAEIFEHEL AYALLHQIFS FNSPVWFNVG TPQPQQVSAC FILSVDDSME SILDWYKEEG 

       190        200        210        220        230        240 
MIFKGGSGAG LNLSRIRSSK ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI 

       250        260        270        280        290        300 
LDVDHPDIEG FIETKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDEFMRA 

       310        320        330        340        350        360 
VESGSAFGLR ARMTGEIIEQ VDAKALFRKM AQAAWACADP GIQYDDTINR WHTCPESGRI 

       370        380        390        400        410        420 
NGSNPCSEYM HLDNTSCNLA SLNLMKFLTD DGEGNQSFDV ERFAKVVELV ITAMDISICF 

       430        440        450        460        470        480 
ADFPTQKIGE NTRAFRQLGI GYANLGALLM ATGHAYDSDG GRAIAGAISS LMTGTSYRRS 

       490        500        510        520        530        540 
AELAAVVGPY DGYARNAAPH NQVMRQHADA NDTAVRMDDL DTPIWAAATE TWQDVLRLGE 

       550        560        570        580        590        600 
KNGFRNAQAS VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR 

       610        620        630        640        650        660 
LGYQAEQIEA IVEHIAEHGN VLDAPGLKTE HYKVFDCAMG ERSISAMGHV RMMAAIQPWI 

       670        680        690        700        710        720 
SGALSKTVNM PESATVEEVE EIYFEAWKMG VKALAIYRDN CKVGQPLSAK TKEKEQDGIA 

       730        740        750        760        770        780 
EKTEDTIRAA VEKVIEYRPV RKRLPKGRPG ITTSFTVGGA EGYMTANSYP DDGLGEVFLK 

       790        800        810        820        830        840 
MSKQGSTLAG MMDAFSIAVS VGLQYGVPLE TYVSKFTNMR FEPAGMTDDP DVRMAQSIVD 

       850        860        870        880        890        900 
NIFRRLALDF LPFETRSALG IHSAEERQRH LDTGSYEQVI EEDELDVEGL AQSAPRQQIP 

       910        920        930        940        950        960 
AVPAAPAEIP APKQAHTSAE LVEMQLGISA DAPLCFSCGT KMQRAGSCYI CEGCGSTSGC 


S 

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References

[1]"Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y.
Microbiology 148:391-404(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ224870 Genomic DNA. Translation: CAA12167.1.

3D structure databases

ProteinModelPortalO54196.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSPR01183. RIBORDTASEM1.
TIGRFAMsTIGR02504. NrdJ_Z. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNRDJ_STRC2
AccessionPrimary (citable) accession number: O54196
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1998
Last modified: April 16, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families