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O54196

- NRDJ_STRC2

UniProt

O54196 - NRDJ_STRC2

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Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

5'-deoxyadenosylcobalamine (coenzyme B12).Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431SubstrateBy similarity
Binding sitei188 – 1881Substrate; via amide nitrogenBy similarity
Active sitei364 – 3641Proton acceptorBy similarity
Active sitei366 – 3661Cysteine radical intermediateBy similarity
Active sitei368 – 3681Proton acceptorBy similarity

GO - Molecular functioni

  1. cobalamin binding Source: UniProtKB-KW
  2. cobalt ion binding Source: InterPro
  3. nucleotide binding Source: UniProtKB-KW
  4. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
OrganismiStreptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Taxonomic identifieri443255 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 961961Vitamin B12-dependent ribonucleotide reductasePRO_0000229028Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi160 ↔ 377Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Expressioni

Developmental stagei

Expressed during the exponential phase of growth.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliO54196.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni159 – 1602Substrate bindingBy similarity
Regioni364 – 3685Substrate bindingBy similarity
Regioni554 – 5585Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view]
PfamiPF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02504. NrdJ_Z. 1 hit.

Sequencei

Sequence statusi: Complete.

O54196-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTETTSGPAR GSRTKGTKAT KGLRIERVHT TPGVHPYDEV VWERRDVVMT
60 70 80 90 100
NWRDGSVNFE QRGVEFPDFW SVNAVNIVTS KYFRGAVGTP QRETGLKQLI
110 120 130 140 150
DRIVKTYRKA GEEYKYFASP ADAEIFEHEL AYALLHQIFS FNSPVWFNVG
160 170 180 190 200
TPQPQQVSAC FILSVDDSME SILDWYKEEG MIFKGGSGAG LNLSRIRSSK
210 220 230 240 250
ELLSSGGNAS GPVSFMRGAD ASAGTIKSGG ATRRAAKMVI LDVDHPDIEG
260 270 280 290 300
FIETKVKEEE KIRALRDAGF DMDLGGDDIT SVQYQNANNS VRVNDEFMRA
310 320 330 340 350
VESGSAFGLR ARMTGEIIEQ VDAKALFRKM AQAAWACADP GIQYDDTINR
360 370 380 390 400
WHTCPESGRI NGSNPCSEYM HLDNTSCNLA SLNLMKFLTD DGEGNQSFDV
410 420 430 440 450
ERFAKVVELV ITAMDISICF ADFPTQKIGE NTRAFRQLGI GYANLGALLM
460 470 480 490 500
ATGHAYDSDG GRAIAGAISS LMTGTSYRRS AELAAVVGPY DGYARNAAPH
510 520 530 540 550
NQVMRQHADA NDTAVRMDDL DTPIWAAATE TWQDVLRLGE KNGFRNAQAS
560 570 580 590 600
VIAPTGTIGL AMSCDTTGLE PDLALVKFKK LVGGGSMQIV NGTVPQALRR
610 620 630 640 650
LGYQAEQIEA IVEHIAEHGN VLDAPGLKTE HYKVFDCAMG ERSISAMGHV
660 670 680 690 700
RMMAAIQPWI SGALSKTVNM PESATVEEVE EIYFEAWKMG VKALAIYRDN
710 720 730 740 750
CKVGQPLSAK TKEKEQDGIA EKTEDTIRAA VEKVIEYRPV RKRLPKGRPG
760 770 780 790 800
ITTSFTVGGA EGYMTANSYP DDGLGEVFLK MSKQGSTLAG MMDAFSIAVS
810 820 830 840 850
VGLQYGVPLE TYVSKFTNMR FEPAGMTDDP DVRMAQSIVD NIFRRLALDF
860 870 880 890 900
LPFETRSALG IHSAEERQRH LDTGSYEQVI EEDELDVEGL AQSAPRQQIP
910 920 930 940 950
AVPAAPAEIP APKQAHTSAE LVEMQLGISA DAPLCFSCGT KMQRAGSCYI
960
CEGCGSTSGC S
Length:961
Mass (Da):104,791
Last modified:November 1, 1998 - v2
Checksum:i9C9D30730047A9F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ224870 Genomic DNA. Translation: CAA12167.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ224870 Genomic DNA. Translation: CAA12167.1 .

3D structure databases

ProteinModelPortali O54196.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR013678. RNR_2_N.
IPR000788. RNR_lg_C.
IPR013344. RNR_NrdJ/NrdZ.
IPR024434. TSCPD_dom.
[Graphical view ]
Pfami PF08471. Ribonuc_red_2_N. 1 hit.
PF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02504. NrdJ_Z. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Streptomyces spp. contain class Ia and class II ribonucleotide reductases: expression analysis of the genes in vegetative growth."
    Borovok I., Kreisberg-Zakarin R., Yanko M., Schreiber R., Myslovati M., Aaslund F., Holmgren A., Cohen G., Aharonowitz Y.
    Microbiology 148:391-404(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602.

Entry informationi

Entry nameiNRDJ_STRC2
AccessioniPrimary (citable) accession number: O54196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: November 1, 1998
Last modified: October 1, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3