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O54161

- EABF_STRCO

UniProt

O54161 - EABF_STRCO

Protein

Extracellular exo-alpha-L-arabinofuranosidase

Gene

abfB

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Involved in the degradation of xylan and is a key enzyme in the complete degradation of the plant cell wall. It has a specific arabinofuranose-debranching activity on xylan from gramineae. Acts synergistically with the xylanases and binds specifically to xylan. From small arabinoxylo-oligosides (ranging from arabinoxylotriose to arabinoxylohexaose), it liberates arabinose and, after prolonged incubation, the purified enzyme exhibits some xylanolytic activity as well By similarity.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

    Pathwayi

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. L-arabinose metabolic process Source: InterPro
    2. xylan catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Keywords - Ligandi

    Lectin

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GH62. Glycoside Hydrolase Family 62.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular exo-alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Short name:
    ABF
    Alternative name(s):
    Arabinosidase
    Arabinoxylan arabinofuranohydrolase
    Gene namesi
    Name:abfB
    Ordered Locus Names:SCO5932
    ORF Names:SC7H1.02
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3737Sequence AnalysisAdd
    BLAST
    Chaini38 – 475438Extracellular exo-alpha-L-arabinofuranosidasePRO_0000008036Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO5932.

    Structurei

    Secondary structure

    1
    475
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi184 – 1863
    Beta strandi198 – 20811
    Beta strandi211 – 2199
    Beta strandi224 – 2296
    Beta strandi232 – 2343
    Helixi235 – 2406
    Beta strandi243 – 2453
    Beta strandi251 – 2588
    Helixi259 – 2613
    Beta strandi263 – 28119
    Beta strandi293 – 2964
    Beta strandi307 – 3148
    Beta strandi316 – 3249
    Beta strandi326 – 33611
    Helixi337 – 3393
    Beta strandi349 – 3535
    Turni356 – 3583
    Beta strandi361 – 3688
    Turni369 – 3724
    Beta strandi374 – 3818
    Beta strandi383 – 39513
    Beta strandi401 – 41313
    Turni414 – 4163
    Beta strandi426 – 4305
    Beta strandi432 – 4343
    Helixi443 – 4453
    Beta strandi447 – 4526
    Helixi461 – 4633
    Beta strandi467 – 4737

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3WMYX-ray1.40A38-475[»]
    3WMZX-ray1.90A38-475[»]
    3WN0X-ray1.90A38-475[»]
    3WN1X-ray2.00A38-475[»]
    3WN2X-ray2.10A38-475[»]
    ProteinModelPortaliO54161.
    SMRiO54161. Positions 39-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 166128Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydr olase 62 family.Curated
    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG81570.
    HOGENOMiHOG000025219.
    OMAiTGCIDQT.
    OrthoDBiEOG6ZH2FF.
    PhylomeDBiO54161.

    Family and domain databases

    InterProiIPR005193. GH62_arabinosidase.
    IPR023296. Glyco_hydro_beta-prop.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF03664. Glyco_hydro_62. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O54161-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHRGSLSRGH TSAVLAAVVA ALAALAALLV ATTPAQAAGS GALRGAGSNR    50
    CLDVLGGSQD DGALLQLYDC WGGTNQQWTS TDTGRLTVYG DKCLDVPGHA 100
    TAPGTRVQIW SCSGGANQQW RVNSDGTVVG VESGLCLEAA GAGTANGTAV 150
    QLWTCNGGGN QKWTGLTGTP PTDGTCALPS TYRWSSTGVL AQPKSGWVAL 200
    KDFTTVTHNG RHLVYGSTSS GSSYGSMVFS PFTNWSDMAS AGQNAMNQAA 250
    VAPTLFYFAP KNIWVLAYQW GSWPFIYRTS SDPTDPNGWS APQPLFTGSI 300
    SGSDTGPIDQ TLIADGQNMY LFFAGDNGKI YRASMPIGNF PGNFGSSYTT 350
    IMSDTKANLF EGVQVYKVQG QNQYLMIVEA MGANGRYFRS FTASSLSGSW 400
    TPQAASEGNP FAGKANSGAT WTNDISHGDL VRDNPDQTMT VDPCNLQFLY 450
    QGKSPNAGGD YNSLPWRPGV LTLRR 475
    Length:475
    Mass (Da):50,046
    Last modified:June 1, 1998 - v1
    Checksum:i47E707FE543CA60D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939125 Genomic DNA. Translation: CAA16189.1.
    PIRiT35697.
    RefSeqiNP_630049.1. NC_003888.3.
    WP_011030541.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAA16189; CAA16189; CAA16189.
    GeneIDi1101374.
    KEGGisco:SCO5932.
    PATRICi23741764. VBIStrCoe124346_6029.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939125 Genomic DNA. Translation: CAA16189.1 .
    PIRi T35697.
    RefSeqi NP_630049.1. NC_003888.3.
    WP_011030541.1. NC_003888.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3WMY X-ray 1.40 A 38-475 [» ]
    3WMZ X-ray 1.90 A 38-475 [» ]
    3WN0 X-ray 1.90 A 38-475 [» ]
    3WN1 X-ray 2.00 A 38-475 [» ]
    3WN2 X-ray 2.10 A 38-475 [» ]
    ProteinModelPortali O54161.
    SMRi O54161. Positions 39-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO5932.

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GH62. Glycoside Hydrolase Family 62.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAA16189 ; CAA16189 ; CAA16189 .
    GeneIDi 1101374.
    KEGGi sco:SCO5932.
    PATRICi 23741764. VBIStrCoe124346_6029.

    Phylogenomic databases

    eggNOGi NOG81570.
    HOGENOMi HOG000025219.
    OMAi TGCIDQT.
    OrthoDBi EOG6ZH2FF.
    PhylomeDBi O54161.

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    InterProi IPR005193. GH62_arabinosidase.
    IPR023296. Glyco_hydro_beta-prop.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF03664. Glyco_hydro_62. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF75005. SSF75005. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiEABF_STRCO
    AccessioniPrimary (citable) accession number: O54161
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3