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O54161

- EABF_STRCO

UniProt

O54161 - EABF_STRCO

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Protein

Extracellular exo-alpha-L-arabinofuranosidase

Gene

abfB

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the degradation of xylan and is a key enzyme in the complete degradation of the plant cell wall. It has a specific arabinofuranose-debranching activity on xylan from gramineae. Acts synergistically with the xylanases and binds specifically to xylan. From small arabinoxylo-oligosides (ranging from arabinoxylotriose to arabinoxylohexaose), it liberates arabinose and, after prolonged incubation, the purified enzyme exhibits some xylanolytic activity as well (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.

Pathwayi

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: UniProtKB-KW

GO - Biological processi

  1. L-arabinose metabolic process Source: InterPro
  2. xylan catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

Keywords - Ligandi

Lectin

Enzyme and pathway databases

UniPathwayiUPA00114.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GH62. Glycoside Hydrolase Family 62.

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular exo-alpha-L-arabinofuranosidase (EC:3.2.1.55)
Short name:
ABF
Alternative name(s):
Arabinosidase
Arabinoxylan arabinofuranohydrolase
Gene namesi
Name:abfB
Ordered Locus Names:SCO5932
ORF Names:SC7H1.02
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence AnalysisAdd
BLAST
Chaini38 – 475438Extracellular exo-alpha-L-arabinofuranosidasePRO_0000008036Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO5932.

Structurei

Secondary structure

1
475
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi184 – 1863Combined sources
Beta strandi198 – 20811Combined sources
Beta strandi211 – 2199Combined sources
Beta strandi224 – 2296Combined sources
Beta strandi232 – 2343Combined sources
Helixi235 – 2406Combined sources
Beta strandi243 – 2453Combined sources
Beta strandi251 – 2588Combined sources
Helixi259 – 2613Combined sources
Beta strandi263 – 28119Combined sources
Beta strandi293 – 2964Combined sources
Beta strandi307 – 3148Combined sources
Beta strandi316 – 3249Combined sources
Beta strandi326 – 33611Combined sources
Helixi337 – 3393Combined sources
Beta strandi349 – 3535Combined sources
Turni356 – 3583Combined sources
Beta strandi361 – 3688Combined sources
Turni369 – 3724Combined sources
Beta strandi374 – 3818Combined sources
Beta strandi383 – 39513Combined sources
Beta strandi401 – 41313Combined sources
Turni414 – 4163Combined sources
Beta strandi426 – 4305Combined sources
Beta strandi432 – 4343Combined sources
Helixi443 – 4453Combined sources
Beta strandi447 – 4526Combined sources
Helixi461 – 4633Combined sources
Beta strandi467 – 4737Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WMYX-ray1.40A38-475[»]
3WMZX-ray1.90A38-475[»]
3WN0X-ray1.90A38-475[»]
3WN1X-ray2.00A38-475[»]
3WN2X-ray2.10A38-475[»]
ProteinModelPortaliO54161.
SMRiO54161. Positions 39-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 166128Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydr olase 62 family.Curated
Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG81570.
HOGENOMiHOG000025219.
InParanoidiO54161.
OMAiTGCIDQT.
OrthoDBiEOG6ZH2FF.
PhylomeDBiO54161.

Family and domain databases

InterProiIPR005193. GH62_arabinosidase.
IPR023296. Glyco_hydro_beta-prop.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF03664. Glyco_hydro_62. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF75005. SSF75005. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O54161-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHRGSLSRGH TSAVLAAVVA ALAALAALLV ATTPAQAAGS GALRGAGSNR
60 70 80 90 100
CLDVLGGSQD DGALLQLYDC WGGTNQQWTS TDTGRLTVYG DKCLDVPGHA
110 120 130 140 150
TAPGTRVQIW SCSGGANQQW RVNSDGTVVG VESGLCLEAA GAGTANGTAV
160 170 180 190 200
QLWTCNGGGN QKWTGLTGTP PTDGTCALPS TYRWSSTGVL AQPKSGWVAL
210 220 230 240 250
KDFTTVTHNG RHLVYGSTSS GSSYGSMVFS PFTNWSDMAS AGQNAMNQAA
260 270 280 290 300
VAPTLFYFAP KNIWVLAYQW GSWPFIYRTS SDPTDPNGWS APQPLFTGSI
310 320 330 340 350
SGSDTGPIDQ TLIADGQNMY LFFAGDNGKI YRASMPIGNF PGNFGSSYTT
360 370 380 390 400
IMSDTKANLF EGVQVYKVQG QNQYLMIVEA MGANGRYFRS FTASSLSGSW
410 420 430 440 450
TPQAASEGNP FAGKANSGAT WTNDISHGDL VRDNPDQTMT VDPCNLQFLY
460 470
QGKSPNAGGD YNSLPWRPGV LTLRR
Length:475
Mass (Da):50,046
Last modified:June 1, 1998 - v1
Checksum:i47E707FE543CA60D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA16189.1.
PIRiT35697.
RefSeqiNP_630049.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAA16189; CAA16189; CAA16189.
GeneIDi1101374.
KEGGisco:SCO5932.
PATRICi23741764. VBIStrCoe124346_6029.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939125 Genomic DNA. Translation: CAA16189.1 .
PIRi T35697.
RefSeqi NP_630049.1. NC_003888.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3WMY X-ray 1.40 A 38-475 [» ]
3WMZ X-ray 1.90 A 38-475 [» ]
3WN0 X-ray 1.90 A 38-475 [» ]
3WN1 X-ray 2.00 A 38-475 [» ]
3WN2 X-ray 2.10 A 38-475 [» ]
ProteinModelPortali O54161.
SMRi O54161. Positions 39-164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO5932.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GH62. Glycoside Hydrolase Family 62.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAA16189 ; CAA16189 ; CAA16189 .
GeneIDi 1101374.
KEGGi sco:SCO5932.
PATRICi 23741764. VBIStrCoe124346_6029.

Phylogenomic databases

eggNOGi NOG81570.
HOGENOMi HOG000025219.
InParanoidi O54161.
OMAi TGCIDQT.
OrthoDBi EOG6ZH2FF.
PhylomeDBi O54161.

Enzyme and pathway databases

UniPathwayi UPA00114 .

Family and domain databases

InterProi IPR005193. GH62_arabinosidase.
IPR023296. Glyco_hydro_beta-prop.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF03664. Glyco_hydro_62. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF75005. SSF75005. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiEABF_STRCO
AccessioniPrimary (citable) accession number: O54161
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3