O54075 (PCL_RHOS4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-coumarate--CoA ligase
Short name=4CL
EC=6.2.1.12

Alternative name(s):
4-coumaroyl-CoA synthase

Gene names

Name:

pcl

Organism

Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

Taxonomic identifier

272943 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts p-coumaric acid into p-coumaryl CoA. This is necessary for the activation of the photoactive yellow protein (PYP) chromophore.
Catalytic activity	ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-coumarate-CoA ligase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	4-coumarate--CoA ligase		PRO_0000193045

Sequences

	Sequence		Length	Mass (Da)
	O54075 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 0296253554395F37	FASTA	411	45,008
	10 20 30 40 50 60 MTAEGPLAPE DRVLDREAIG RLCVSLIAAE QQDLLREGRV GHHQMIGARL LTAGHPSPDD 70 80 90 100 110 120 LLIDEDTLGL DSLLMLSLVT RVAGFFHLSD SNTEDYLLVR RRLGEWVDLI DHHHTLMGPK 130 140 150 160 170 180 ARFTFATSGS TAGPKPVTHS AAALLSEGQA IAKILTERPP EVRRVLSCVP AHHIYGFLWS 190 200 210 220 230 240 CLFPSRRGLE AKQLANLSAS GIMRHARSGD LVVGTPFIWE QFADLDYRLP GDVVGVTSGA 250 260 270 280 290 300 PSTAETWRCA SALGPARMLD IYGSTETGGI GWRERRDDPF RTLPDLACFH DTLSRLGRRL 310 320 330 340 350 360 DLQDEIAWDK DGGFTILGRK DEILQVAGSN VSPAAVRDIL LRNPRVRDAA VRLDGRRLKA 370 380 390 400 410 VISVAEGADE AEIEIELRAT AARHLPAPAR PDRFLFATEL PRTGAGKLAD W

References

[1]

"Sequence, chromophore extraction and 3-D model of the photoactive yellow protein from Rhodobacter sphaeroides." Kort R., Phillips-Jones M.K., Van Aalten D.M.F., Haker A., Hoffer S.M., Hellingwerf K.J., Crielaard W. Biochim. Biophys. Acta 1385:1-6(1998) [PubMed: 9630474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ002398 Genomic DNA. Translation: CAA05380.1.
3D structure databases
ProteinModelPortal	O54075.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0365.
Family and domain databases
InterPro	IPR012743. 4_coum_CoA_lig. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 2 hits. [Graphical view]
TIGRFAMs	TIGR02372. 4_coum_CoA_lig. 1 hit.
PROSITE	PS00455. AMP_BINDING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCL_RHOS4

Accession

Primary (citable) accession number: O54075

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	June 1, 1998
Last modified:	November 30, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program