Reviewed, UniProtKB/Swiss-Prot O54068 (UDG_RHIME)

Names and origin

Protein names

Recommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22

Gene names

Name:	rkpK
Ordered Locus Names:	R01082
ORF Names:	SMc02641

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-glucuronate biosynthesis; UDP-glucuronate from UDP-glucose: step 1/1. Capsule biogenesis; capsule polysaccharide biosynthesis.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	UDP-glucose 6-dehydrogenase		PRO_0000074050
Sites
	Active site	265	1	By similarity
Experimental info
	Sequence conflict	109	1	A → S in CAA10918. Ref.1
	Sequence conflict	142	1	A → V in CAA10918. Ref.1
	Sequence conflict	318	1	I → Y in CAA10918. Ref.1
	Sequence conflict	335	1	S → T in CAA10918. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O54068-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 2D458D39A1D8EEC1	FASTA	437	47,122
	10 20 30 40 50 60 MKITMIGAGY VGLVSGVCFA DFGHDVVCVD KDEGKISALK KGQIPIFEPG LDHLVASNVA 70 80 90 100 110 120 SGRLNFTDDL KTAVAASDVV FIAVGTPSRR GDGHADLSYV YAAAREIAAN LQGFTVVVTK 130 140 150 160 170 180 STVPVGTGDE VERIIRETNP AADVTVVSNP EFLREGAAIE DFKRPDRIVI GVDGSDGRAR 190 200 210 220 230 240 EVMTEVYRPL YLNQSPLVFT TRRTSELIKY AGNAFLAMKI TFINEIADLC EKVGANVQDV 250 260 270 280 290 300 ARGIGLDGRI GSKFLHAGPG YGGSCFPKDT LALVKTAQDH DTPVRLVETT VAVNDNRKRA 310 320 330 340 350 360 MGRKVIAAAG GDIRGSKIAV LGLTFKPNTD DMRDSPAIAV VQALQDAGAR VTGYDPEGME 370 380 390 400 410 420 NARKLIEGLD CARDPYEAAA EADALVIITE WNEFRALDFD RLKSTMKTPL LVDLRNIYRK 430 DEVAKHGFRY ASIGRPD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule: the rkpK gene encodes a UDP-glucose dehydrogenase." Kereszt A., Kiss E., Reuhs B.L., Carlson R.W., Kondorosi A., Putnoky P. J. Bacteriol. 180:5426-5431(1998) [PubMed: 9765575] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ222661 Genomic DNA. Translation: CAA10918.1. AL591688 Genomic DNA. Translation: CAC45661.1.
PIR	T46573.
RefSeq	NP_385188.1.
3D structure databases
HSSP	HSSP built from PDB template 1MV8 based on UniProtKB P11759.
ModBase	Search...
Genome annotation databases
GeneID	1232726.
GenomeReviews	Gene locus R01082 in contig AL591688_GR.
KEGG	sme:SMc02641.
NMPDR	fig|266834.1.peg.2376.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	O54068.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC02641-MON.
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017476. Nucleotide_sugar_DH. IPR001100. Pyr_nuc-diS_OxRdtase. IPR014027. UDP-Glc/GDP-Man_DHase_C. IPR014026. UDP-Glc/GDP-Man_DHase_dimer. IPR014028. UDP-Glc/GDP-Man_DHase_dimer-bd. IPR001732. UDP-Glc/GDP-Man_DHase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit. G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHER	PTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
PRINTS	PR00411. PNDRDTASEI.
ProtoNet	Search...

Entry information

Entry name

UDG_RHIME

Accession

Primary (citable) accession number: O54068

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 25, 2008
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)