O54068 (UDG_RHIME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 6-dehydrogenase
Short name=UDP-Glc dehydrogenase
Short name=UDP-GlcDH
Short name=UDPGDH
EC=1.1.1.22

Gene names

Name:	rkpK
Ordered Locus Names:	R01082
ORF Names:	SMc02641

Organism

Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

266834 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › Rhizobium meliloti

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1. Capsule biogenesis; capsule polysaccharide biosynthesis.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	UDP-glucuronate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway capsule polysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	UDP-glucose 6-dehydrogenase		PRO_0000074050
Sites
	Active site	265	1	By similarity
Experimental info
	Sequence conflict	109	1	A → S in CAA10918. Ref.1
	Sequence conflict	142	1	A → V in CAA10918. Ref.1
	Sequence conflict	318	1	I → Y in CAA10918. Ref.1
	Sequence conflict	335	1	S → T in CAA10918. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O54068 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 2D458D39A1D8EEC1	FASTA	437	47,122
	10 20 30 40 50 60 MKITMIGAGY VGLVSGVCFA DFGHDVVCVD KDEGKISALK KGQIPIFEPG LDHLVASNVA 70 80 90 100 110 120 SGRLNFTDDL KTAVAASDVV FIAVGTPSRR GDGHADLSYV YAAAREIAAN LQGFTVVVTK 130 140 150 160 170 180 STVPVGTGDE VERIIRETNP AADVTVVSNP EFLREGAAIE DFKRPDRIVI GVDGSDGRAR 190 200 210 220 230 240 EVMTEVYRPL YLNQSPLVFT TRRTSELIKY AGNAFLAMKI TFINEIADLC EKVGANVQDV 250 260 270 280 290 300 ARGIGLDGRI GSKFLHAGPG YGGSCFPKDT LALVKTAQDH DTPVRLVETT VAVNDNRKRA 310 320 330 340 350 360 MGRKVIAAAG GDIRGSKIAV LGLTFKPNTD DMRDSPAIAV VQALQDAGAR VTGYDPEGME 370 380 390 400 410 420 NARKLIEGLD CARDPYEAAA EADALVIITE WNEFRALDFD RLKSTMKTPL LVDLRNIYRK 430 DEVAKHGFRY ASIGRPD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule: the rkpK gene encodes a UDP-glucose dehydrogenase." Kereszt A., Kiss E., Reuhs B.L., Carlson R.W., Kondorosi A., Putnoky P. J. Bacteriol. 180:5426-5431(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 41.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.
[3]	"The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J. Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ222661 Genomic DNA. Translation: CAA10918.1. AL591688 Genomic DNA. Translation: CAC45661.1.
PIR	T46573.
RefSeq	NP_385188.1. NC_003047.1.
3D structure databases
ProteinModelPortal	O54068.
ModBase	Search...
Protein-protein interaction databases
STRING	266834.SMc02641.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAC45661; CAC45661; SMc02641.
GeneID	1232726.
KEGG	sme:SMc02641.
PATRIC	23631459. VBISinMel96828_2490.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1004.
HOGENOM	HOG000153773.
KO	K00012.
OMA	VRCVERD.
ProtClustDB	CLSK800851.
Enzyme and pathway databases
BioCyc	SMEL266834:GJF6-1107-MONOMER.
UniPathway	UPA00038; UER00491. UPA00934.
Family and domain databases
Gene3D	3.40.50.720. 2 hits.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMART	SM00984. UDPG_MGDP_dh_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UDG_RHIME

Accession

Primary (citable) accession number: O54068

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 100 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program