Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrogen regulatory protein P-II

Gene

glnB

Organism
Rhizobium etli (strain CFN 42 / ATCC 51251)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P-II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deuridylated and promotes the adenylation of GS. P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4062-MONOMER.
RETL347834:GJJ0-3992-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogen regulatory protein P-II
Gene namesi
Name:glnB
Synonyms:glnK
Ordered Locus Names:RHE_CH03974
OrganismiRhizobium etli (strain CFN 42 / ATCC 51251)
Taxonomic identifieri347834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium
Proteomesi
  • UP000001936 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 112112Nitrogen regulatory protein P-IIPRO_0000139782Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511O-UMP-tyrosinePROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi347834.RHE_CH03974.

Structurei

3D structure databases

ProteinModelPortaliO54053.
SMRiO54053. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P(II) protein family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
KOiK04752.
OMAiSRVTGHG.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O54053-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIVMAIIKP FKLDEVREAL TAIGIQGLTV TEVKGYGRQK GHTEIYRGTE
60 70 80 90 100
YAVSFLPKLK IEIAVASELV DRAVEAIAAS AKTGQIGDGK IFVYSIDHAV
110
RIRTGETDSE AL
Length:112
Mass (Da):12,178
Last modified:September 21, 2011 - v3
Checksum:iDFAE526476C82DA3
GO

Sequence cautioni

The sequence CAA05496 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti50 – 501E → V in CAA05496 (PubMed:9487694).Curated
Sequence conflicti78 – 781A → G in CAA05496 (PubMed:9487694).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002489 Genomic DNA. Translation: CAA05496.1. Different initiation.
CP000133 Genomic DNA. Translation: ABC92719.1.

Genome annotation databases

EnsemblBacteriaiABC92719; ABC92719; RHE_CH03974.
KEGGiret:RHE_CH03974.
PATRICi23089688. VBIRhiEtl108884_4478.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002489 Genomic DNA. Translation: CAA05496.1. Different initiation.
CP000133 Genomic DNA. Translation: ABC92719.1.

3D structure databases

ProteinModelPortaliO54053.
SMRiO54053. Positions 1-112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi347834.RHE_CH03974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC92719; ABC92719; RHE_CH03974.
KEGGiret:RHE_CH03974.
PATRICi23089688. VBIRhiEtl108884_4478.

Phylogenomic databases

eggNOGiENOG4108YZA. Bacteria.
COG0347. LUCA.
HOGENOMiHOG000017847.
KOiK04752.
OMAiSRVTGHG.

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-4062-MONOMER.
RETL347834:GJJ0-3992-MONOMER.

Family and domain databases

Gene3Di3.30.70.120. 1 hit.
InterProiIPR002187. N-reg_PII.
IPR011322. N-reg_PII-like_a/b.
IPR015867. N-reg_PII/ATP_PRibTrfase_C.
IPR017918. N-reg_PII_CS.
[Graphical view]
PfamiPF00543. P-II. 1 hit.
[Graphical view]
PIRSFiPIRSF039144. GlnB. 1 hit.
PRINTSiPR00340. PIIGLNB.
SMARTiSM00938. P-II. 1 hit.
[Graphical view]
SUPFAMiSSF54913. SSF54913. 1 hit.
PROSITEiPS00638. PII_GLNB_CTER. 1 hit.
PS51343. PII_GLNB_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLNB_RHIEC
AccessioniPrimary (citable) accession number: O54053
Secondary accession number(s): Q2K367
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 21, 2011
Last modified: September 7, 2016
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.