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Protein

Probable inositol 1-monophosphatase ImpA

Gene

impA

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the dephosphorylation of inositol 1-phosphate (I-1-P) to yield free myo-inositol, a key metabolite in mycobacteria.By similarity

Catalytic activityi

Myo-inositol phosphate + H2O = myo-inositol + phosphate.

Cofactori

Mg2+By similarity

Pathwayi: myo-inositol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Probable inositol 1-monophosphatase ImpA (impA), Inositol-1-monophosphatase SuhB (suhB)
This subpathway is part of the pathway myo-inositol biosynthesis, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes myo-inositol from D-glucose 6-phosphate, the pathway myo-inositol biosynthesis and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Magnesium 1By similarity
Binding sitei69 – 691SubstrateBy similarity
Metal bindingi85 – 851Magnesium 1By similarity
Metal bindingi85 – 851Magnesium 2By similarity
Metal bindingi87 – 871Magnesium 1; via carbonyl oxygenBy similarity
Metal bindingi88 – 881Magnesium 2By similarity
Binding sitei187 – 1871SubstrateBy similarity
Metal bindingi216 – 2161Magnesium 2By similarity
Binding sitei216 – 2161SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMTBRV:RV1604-MONOMER.
UniPathwayiUPA00823; UER00788.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable inositol 1-monophosphatase ImpA (EC:3.1.3.25)
Short name:
I-1-Pase
Short name:
IMPase
Short name:
Inositol-1-phosphatase
Gene namesi
Name:impA
Ordered Locus Names:Rv1604
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1604.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Strains lacking this gene show no difference in colony morphology and no differences in levels of phosphatidylinosotol mannosides (PIMs), lipomannan (LM), lipoarabinomannan (LAM) or mycothiol (in the absence of exogenous inositol).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Probable inositol 1-monophosphatase ImpAPRO_0000404321Add
BLAST

Proteomic databases

PaxDbiO53907.
PRIDEiO53907.

Expressioni

Inductioni

When comparing gene expression levels of the four IMPase family genes in exponential cultures of M.tuberculosis, the level of cysQ is the highest, almost equal to sigA; impA and impC are expressed at approximately 40% of this level, while suhB is lowest, at 12% of the cysQ level.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv1604.

Structurei

3D structure databases

ProteinModelPortaliO53907.
SMRiO53907. Positions 28-257.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni87 – 904Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the inositol monophosphatase family.Curated

Phylogenomic databases

eggNOGiENOG4108MWI. Bacteria.
COG0483. LUCA.
HOGENOMiHOG000282238.
InParanoidiO53907.
KOiK01092.
OMAiNGEAHEA.
PhylomeDBiO53907.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O53907-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLDSLVAPL VEQASAILDA ATALFLVGHR ADSAVRKKGN DFATEVDLAI
60 70 80 90 100
ERQVVAALVA ATGIEVHGEE FGGPAVDSRW VWVLDPIDGT INYAAGSPLA
110 120 130 140 150
AILLGLLHDG VPVAGLTWMP FTDPRYTAVA GGPLIKNGVP QPPLADAELA
160 170 180 190 200
NVLVGVGTFS ADSRGQFPGR YRLAVLEKLS RVSSRLRMHG STGIDLVFVA
210 220 230 240 250
DGILGGAISF GGHVWDHAAG VALVRAAGGV VTDLAGQPWT PASRSALAGP
260 270
PRVHAQILEI LGSIGEPEDY
Length:270
Mass (Da):27,979
Last modified:June 1, 1998 - v1
Checksum:iD86E14851E4DC8CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44368.1.
PIRiC70819.
RefSeqiNP_216120.1. NC_000962.3.
WP_003911572.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiCCP44368; CCP44368; Rv1604.
GeneIDi885567.
KEGGimtu:Rv1604.
PATRICi18152107. VBIMycTub87468_1786.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP44368.1.
PIRiC70819.
RefSeqiNP_216120.1. NC_000962.3.
WP_003911572.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliO53907.
SMRiO53907. Positions 28-257.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1604.

Proteomic databases

PaxDbiO53907.
PRIDEiO53907.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP44368; CCP44368; Rv1604.
GeneIDi885567.
KEGGimtu:Rv1604.
PATRICi18152107. VBIMycTub87468_1786.

Organism-specific databases

TubercuListiRv1604.

Phylogenomic databases

eggNOGiENOG4108MWI. Bacteria.
COG0483. LUCA.
HOGENOMiHOG000282238.
InParanoidiO53907.
KOiK01092.
OMAiNGEAHEA.
PhylomeDBiO53907.

Enzyme and pathway databases

UniPathwayiUPA00823; UER00788.
BioCyciMTBRV:RV1604-MONOMER.

Family and domain databases

InterProiIPR000760. Inositol_monophosphatase.
IPR020550. Inositol_monophosphatase_CS.
[Graphical view]
PANTHERiPTHR20854. PTHR20854. 1 hit.
PfamiPF00459. Inositol_P. 1 hit.
[Graphical view]
PRINTSiPR00377. IMPHPHTASES.
PROSITEiPS00630. IMP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMPA_MYCTU
AccessioniPrimary (citable) accession number: O53907
Secondary accession number(s): L0TA49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 8, 2011
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.