O53871 (Y0859_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative acyltransferase Rv0859
EC=2.3.1.-

Gene names

Name:	fadA
Ordered Locus Names:	Rv0859

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the thiolase family.

Ontologies

Keywords
Molecular function	Acyltransferase Transferase
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 403	403	Putative acyltransferase Rv0859		PRO_0000395883
Sites
	Active site	92	1	Acyl-thioester intermediate By similarity
	Active site	359	1	Proton acceptor By similarity
	Active site	389	1	Proton acceptor By similarity
Amino acid modifications
	Cross-link	189		Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein pup)

Sequences

	Sequence		Length	Mass (Da)
	O53871 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 840EABC43EB7FE94	FASTA	403	42,414
	10 20 30 40 50 60 MSEEAFIYEA IRTPRGKQKN GSLHEVKPLS LVVGLIDELR KRHPDLDENL ISDVILGCVS 70 80 90 100 110 120 PVGDQGGDIA RAAVLASGMP VTSGGVQLNR FCASGLEAVN TAAQKVRSGW DDLVLAGGVE 130 140 150 160 170 180 SMSRVPMGSD GGAMGLDPAT NYDVMFVPQS IGADLIATIE GFSREDVDAY ALRSQQKAAE 190 200 210 220 230 240 AWSGGYFAKS VVPVRDQNGL LILDHDEHMR PDTTKEGLAK LKPAFEGLAA LGGFDDVALQ 250 260 270 280 290 300 KYHWVEKINH VHTGGNSSGI VDGAALVMIG SAAAGKLQGL TPRARIVATA TSGADPVIML 310 320 330 340 350 360 TGPTPATRKV LDRAGLTVDD IDLFELNEAF ASVVLKFQKD LNIPDEKLNV NGGAIAMGHP 370 380 390 400 LGATGAMILG TMVDELERRN ARRALITLCI GGGMGVATII ERV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis." Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H. PLoS ONE 5:E8589-E8589(2010) [PubMed: 20066036] [Abstract] Cited for: PUPYLATION AT LYS-189, IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 25618 / H37Rv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842574 Genomic DNA. Translation: CAA17665.1.
PIR	C70815.
RefSeq	NP_215374.1. NC_000962.2.
3D structure databases
HSSP	HSSP built from PDB template 1AFW based on UniProtKB P27796.
ProteinModelPortal	O53871.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000002324; EBMYCP00000002324; EBMYCG00000002322.
GeneID	885774.
GenomeReviews	Gene locus Rv0859 in contig AL123456_GR.
KEGG	mtu:Rv0859.
PATRIC	18150435. VBIMycTub87468_0960.
Organism-specific databases
TubercuList	Rv0859.
Phylogenomic databases
GeneTree	EBGT00070000031748.
HOGENOM	HBG370930.
OMA	QAFEIDK.
PhylomeDB	O53871.
ProtClustDB	PRK08242.
Family and domain databases
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K00626.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y0859_MYCTU

Accession

Primary (citable) accession number: O53871

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 10, 2010
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program