O53770 (PNCB2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate phosphoribosyltransferase pncB2
Short name=NAPRTase pncB2
EC=2.4.2.11

Gene names

Name:	pncB2
Ordered Locus Names:	Rv0573c, MT0601

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) By similarity.
Catalytic activity	Beta-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.
Induction	A member of the dormancy regulon. Induced in response to reduced oxygen tension (hypoxia), low levels of nitric oxide (NO) and carbon monoxide (CO). It is hoped that this regulon will give insight into the latent, or dormant phase of infection. Ref.3 Ref.4 Ref.5
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NAD salvage Inferred from mutant phenotype PubMed 18490451. Source: MTBBASE nicotinate nucleotide salvage Inferred from electronic annotation. Source: InterPro response to host Inferred from expression pattern PubMed 18490451. Source: MTBBASE response to hypoxia Inferred from expression pattern PubMed 18490451. Source: MTBBASE
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay PubMed 15525680. Source: MTBBASE
Molecular_function	nicotinamide phosphoribosyltransferase activity Inferred from direct assay PubMed 18490451. Source: MTBBASE nicotinate phosphoribosyltransferase activity Inferred from direct assay PubMed 18490451. Source: MTBBASE nicotinate-nucleotide diphosphorylase (carboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Nicotinate phosphoribosyltransferase pncB2		PRO_0000392685

Sequences

	Sequence		Length	Mass (Da)
	O53770 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9D0B8745F869533A	FASTA	463	50,509
	10 20 30 40 50 60 MAIRQHVGAL FTDLYEVTMA QAYWAERMSG TAVFEIFFRK LPPGRSYIMA AGLADVVEFL 70 80 90 100 110 120 EAFRFDEQDL RYLRGLGQFS DEFLRWLAGV RFTGDVWAAP EGTVIFPNEP AVQLIAPIIE 130 140 150 160 170 180 AQLVETFVLN QIHLQSVLAS KAARVVAAAR GRPVVDFGAR RAHGTDAACK VARTSYLAGA 190 200 210 220 230 240 AGTSNLLAAR QYGIPTFGTM AHSFVQAFDS EVAAFEAFAR LYPATMLLVD TYDTLRGVDH 250 260 270 280 290 300 VIELAKRLGN RFDVRAVRLD SGDLDELSKA TRARLDTAGL EQVEIFASSG LDENRIAALL 310 320 330 340 350 360 AARCPIDGFG VGTQLVVAQD APALDMAYKL VAYDGSGRTK FSSGKVIYPG RKQVFRKLEH 370 380 390 400 410 420 GVFCGDTLGE HGENLPGDPL LVPIMTNGRR IRQHAPTLDG ARDWARQQID ALPPELRSLE 430 440 450 460 DTGYSYPVAV SDRIVGELAR LRHADTAEAH PGSNVVGAKA KRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program." Voskuil M.I., Schnappinger D., Visconti K.C., Harrell M.I., Dolganov G.M., Sherman D.R., Schoolnik G.K. J. Exp. Med. 198:705-713(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY NITRIC OXIDE (NO) AND BY HYPOXIA, DORMANCY REGULON. Strain: 1254, ATCC 25618 / H37Rv and CDC 1551 / Oshkosh.
[4]	"Mycobacterium tuberculosis senses host-derived carbon monoxide during macrophage infection." Shiloh M.U., Manzanillo P., Cox J.S. Cell Host Microbe 3:323-330(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY CARBON MONOXIDE (CO). Strain: ATCC 35801 / TMC 107 / Erdman.
[5]	"Heme oxygenase-1-derived carbon monoxide induces the Mycobacterium tuberculosis dormancy regulon." Kumar A., Deshane J.S., Crossman D.K., Bolisetty S., Yan B.S., Kramnik I., Agarwal A., Steyn A.J. J. Biol. Chem. 283:18032-18039(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY CARBON MONOXIDE (CO), DORMANCY REGULON. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000516 Genomic DNA. Translation: AAK44824.1. BX842573 Genomic DNA. Translation: CAA17444.1. AL123456 Genomic DNA. Translation: CCP43311.1.
PIR	D70933.
RefSeq	NP_215087.1. NC_000962.3. NP_335010.1. NC_002755.2. YP_006513907.1. NC_018143.1.
3D structure databases
ProteinModelPortal	O53770.
SMR	O53770. Positions 10-431.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv0573c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK44824; AAK44824; MT0601.
GeneID	13318449. 887716. 924994.
KEGG	mtc:MT0601. mtu:Rv0573c. mtv:RVBD_0573c.
PATRIC	18123022. VBIMycTub22151_0644.
Organism-specific databases
TubercuList	Rv0573c.
Phylogenomic databases
eggNOG	COG1488.
HOGENOM	HOG000001113.
KO	K00763.
OMA	PDNGSFV.
ProtClustDB	PRK12484.
Enzyme and pathway databases
UniPathway	UPA00253; UER00457.
Family and domain databases
InterPro	IPR015977. Nic_PRibTrfase-like. IPR007229. Nic_PRibTrfase-rel. IPR006405. Nic_PRibTrfase_put. IPR002638. Quinolinate_PRibosylTrfase_C. [Graphical view]
PANTHER	PTHR11098:SF1. PTHR11098:SF1. 1 hit.
Pfam	PF04095. NAPRTase. 1 hit. [Graphical view]
PIRSF	PIRSF000484. NAPRT. 1 hit.
SUPFAM	SSF51690. Q_phspho_trans. 1 hit.
TIGRFAMs	TIGR01513. NAPRTase_put. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PNCB2_MYCTU

Accession

Primary (citable) accession number: O53770
Secondary accession number(s): L0T470, Q7D9M0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 23, 2010
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program