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Protein

Trehalose 2-sulfotransferase

Gene

stf0

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the sulfuryl group transfer from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to trehalose, leading to trehalose-2-sulfate (T2S). The sulfation of trehalose is the first step in the biosynthesis of sulfolipid-1 (SL-1), a major cell wall glycolipid and the most abundant sulfated metabolite found in Mycobacterium tuberculosis, that is a potential virulence factor thought to mediate host-pathogen interactions.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + alpha,alpha-trehalose = adenosine 3',5'-bisphosphate + 2-O-sulfo-alpha,alpha-trehalose.1 Publication

Pathwayi: Glycolipid metabolism

This protein is involved in Glycolipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in Glycolipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei14alpha,alpha-trehaloseBy similarity1
Active sitei36Proton acceptorBy similarity1
Binding sitei48alpha,alpha-trehalose; via carbonyl oxygenBy similarity1
Binding sitei53alpha,alpha-trehaloseBy similarity1

GO - Molecular functioni

  • 3'-phosphoadenosine 5'-phosphosulfate binding Source: MTBBASE
  • protein homodimerization activity Source: MTBBASE
  • sulfotransferase activity Source: MTBBASE

GO - Biological processi

  • 3'-phosphoadenosine 5'-phosphosulfate metabolic process Source: MTBBASE
  • sulfolipid biosynthetic process Source: MTBBASE
  • trehalose metabolic process Source: MTBBASE

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciMetaCyc:G185E-4418-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose 2-sulfotransferase1 Publication (EC:2.8.2.371 Publication)
Gene namesi
Name:stf01 Publication
Ordered Locus Names:Rv0295cImported, LH57_01610Imported
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome
  • UP000031768 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0295c.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • plasma membrane Source: MTBBASE

Pathology & Biotechi

Disruption phenotypei

Loss of T2S and SL-1 formation. Unsulfated acylated precursors of SL-1 are not detected, which indicates that Stf0 acts before lipid addition to trehalose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004347821 – 267Trehalose 2-sulfotransferaseAdd BLAST267

Proteomic databases

PaxDbiO53699.
PRIDEiO53699.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MTBBASE

Protein-protein interaction databases

STRINGi83332.Rv0295c.

Structurei

3D structure databases

ProteinModelPortaliO53699.
SMRiO53699.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 39alpha,alpha-trehalose bindingBy similarity7

Sequence similaritiesi

Belongs to the Stf0 sulfotransferase family.Curated

Phylogenomic databases

eggNOGiENOG4105WW7. Bacteria.
COG4424. LUCA.
HOGENOMiHOG000070040.
KOiK21014.
OMAiAVSMWRA.
PhylomeDBiO53699.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR015124. Stf0.
IPR024628. Sulfotransferase_Stf0_dom.
PfamiView protein in Pfam
PF09037. Sulphotransf. 1 hit.
PIRSFiPIRSF021497. Sulphotransferase_Stf0. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

O53699-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRAVRPYLV LATQRSGSTL LVESLRATGC AGEPQEFFQY LPSTGMAPQP
60 70 80 90 100
REWFAGVDDD TILQLLDPLD PGTPDTATPV AWREHVRTSG RTPNGVWGGK
110 120 130 140 150
LMWNQTALLQ QRAAQLPDRS GDGLRAAIRD VIGNEPVFVH VHRPDVVSQA
160 170 180 190 200
VSFWRAVQTQ VWRGHPDPKR DSQAVYHAGA IAHIIRNLRD QENGWRAWFA
210 220 230 240 250
EEGIDPIDIA YPVLWRNLTA IVASVLDAIG QDPKLAPAPM LERQANQRSD
260
EWVDRYRAEA PRLGLPT
Length:267
Mass (Da):29,775
Last modified:June 1, 1998 - v1
Checksum:iE60286DC61C5267C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP43025.1.
CP009480 Genomic DNA. Translation: AIR13018.1.
RefSeqiNP_214809.1. NC_000962.3.
WP_003401540.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiAIR13018; AIR13018; LH57_01610.
CCP43025; CCP43025; Rv0295c.
GeneIDi886596.
KEGGimtu:Rv0295c.
mtv:RVBD_0295c.
PATRICifig|83332.111.peg.331.

Similar proteinsi

Entry informationi

Entry nameiSTF0_MYCTU
AccessioniPrimary (citable) accession number: O53699
Secondary accession number(s): F2GM99, I6Y7G4, Q7DA29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 11, 2015
Last sequence update: June 1, 1998
Last modified: June 7, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families