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Protein

Multidrug efflux ATP-binding/permease protein Rv0194

Gene

Rv0194

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Overexpression in M. smegmatis increases resistance to erythromycin, ampicillin, novobiocin and vancomycin. It also reduces accumulation of ethidium bromide in the cell.1 Publication

Enzyme regulationi

Efflux is inhibited by reserpine.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi367 – 3748ATPPROSITE-ProRule annotation
Nucleotide bindingi976 – 9838ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • efflux transmembrane transporter activity Source: MTBBASE
  • lipid-transporting ATPase activity Source: GO_Central

GO - Biological processi

  • antibiotic transport Source: MTBBASE
  • lipid transport Source: GOC
  • response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMTBRV:RV0194-MONOMER.

Protein family/group databases

TCDBi3.A.1.106.6. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug efflux ATP-binding/permease protein Rv0194Curated (EC:3.6.3.-Curated)
Gene namesi
Ordered Locus Names:Rv0194Imported, RVBD_0194Imported
ORF Names:P425_00203Imported
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv0194.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4021HelicalSequence analysisAdd
BLAST
Transmembranei56 – 7621HelicalSequence analysisAdd
BLAST
Transmembranei130 – 15021HelicalSequence analysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence analysisAdd
BLAST
Transmembranei258 – 27821HelicalSequence analysisAdd
BLAST
Transmembranei279 – 29921HelicalSequence analysisAdd
BLAST
Transmembranei628 – 64821HelicalSequence analysisAdd
BLAST
Transmembranei660 – 68021HelicalSequence analysisAdd
BLAST
Transmembranei743 – 76321HelicalSequence analysisAdd
BLAST
Transmembranei765 – 78521HelicalSequence analysisAdd
BLAST
Transmembranei847 – 86721HelicalSequence analysisAdd
BLAST
Transmembranei878 – 89821HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MTBBASE
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11941194Multidrug efflux ATP-binding/permease protein Rv0194PRO_0000432861Add
BLAST

Proteomic databases

PaxDbiO53645.

Interactioni

Protein-protein interaction databases

STRINGi83332.Rv0194.

Structurei

3D structure databases

ProteinModelPortaliO53645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 301281ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini334 – 568235ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini628 – 910283ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini942 – 1177236ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The ATP-binding domains (NBD) and the transmembrane domains (TMD) are fused.Curated

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
KOiK18894.
OMAiMAVLTYY.
OrthoDBiEOG6T7N3V.
PhylomeDBiO53645.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O53645-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTNCWWRLS GYVMRHRRDL LLGFGAALAG TVIAVLVPLV TKRVIDDAIA
60 70 80 90 100
ADHRPLAPWA VVLVAAAGAT YLLMYVRRYY GGRIAHLVQH DLRMDAFQAL
110 120 130 140 150
LRWDGRQQDR WSSGQLIVRT TNDLQLVQAL LFDVPNVLRH VLTLLLGVAV
160 170 180 190 200
MTWLSVPLAL LAVLLVPVIG LIAHRSRRLL AAATHCAQEH KAAVTGVVDA
210 220 230 240 250
AVCGIRVVKA FGQEERETVK LVTASRALYA AQLRVARLNA HFGPLLQTLP
260 270 280 290 300
ALGQMAVFAL GGWMAAQGSI TVGTFVAFWA CLTLLARPAC DLAGMLTIAQ
310 320 330 340 350
QARAGAVRVL ELIDSRPTLV DGTKPLSPEA RLSLEFQRVS FGYVADRPVL
360 370 380 390 400
REISLSVRAG ETLAVVGAPG SGKSTLASLA TRCYDVTQGA VRIGGQDVRE
410 420 430 440 450
LTLDSLRSAI GLVPEDAVLF SGTIGANIAY GRPDATPEQI ATAARAAHIE
460 470 480 490 500
EFVNTLPDGY QTAVGARGLT LSGGQRQRIA LARALLHQPR LLIMDDPTSA
510 520 530 540 550
VDAVIECGIQ EVLREAIADR TAVIFTRRRS MLTLADRVAV LDSGRLLDVG
560 570 580 590 600
TPDEVWERCP RYRELLSPAP DLADDLVVAE RSPVCRPVAG LGTKAAQHTN
610 620 630 640 650
VHNPGPHDHP PGPDPLRRLL REFRGPLALS LLLVAVQTCA GLLPPLLIRH
660 670 680 690 700
GIDVGIRRHV LSALWWAALA GTATVVIRWV VQWGSAMVAG YTGEQVLFRL
710 720 730 740 750
RSVVFAHAQR LGLDAFEDDG DAQIVTAVTA DVEAIVAFLR TGLVVAVISV
760 770 780 790 800
VTLVGILVAL LAIRARLVLL IFTTMPVLAL ATWQFRRASN WTYRRARHRL
810 820 830 840 850
GTVTATLREY AAGLRIAQAF RAEYRGLQSY FAHSDDYRRL GVRGQRLLAL
860 870 880 890 900
YYPFVALLCS LATTLVLLDG AREVRAGVIS VGALVTYLLY IELLYTPIGE
910 920 930 940 950
LAQMFDDYQR AAVAAGRIRS LLSTRTPSSP AARPVGTLRG EVVFDAVHYS
960 970 980 990 1000
YRTREVPALA GINLRIPAGQ TVVFVGSTGS GKSTLIKLVA RFYDPTHGTV
1010 1020 1030 1040 1050
RVDGCDLREF DVDGYRNRLG IVTQEQYVFA GTVRDAIAYG RPDATDAQVE
1060 1070 1080 1090 1100
RAAREVGAHP MITALDNGYL HQVTAGGRNL SAGQLQLLAL ARARLVDPDI
1110 1120 1130 1140 1150
LLLDEATVAL DPATEAVVQR ATLTLAARRT TLIVAHGLAI AEHADRIVVL
1160 1170 1180 1190
EHGTVVEDGA HTELLAAGGH YSRLWAAHTR LCSPEITQLQ CIDA
Length:1,194
Mass (Da):129,251
Last modified:June 1, 1998 - v1
Checksum:i079950FB1BEB581E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42922.1.
CP003248 Genomic DNA. Translation: AFN48045.1.
JLDD01000001 Genomic DNA. Translation: KBJ41303.1.
RefSeqiNP_214708.1. NC_000962.3.
WP_003911097.1. NZ_KK339370.1.

Genome annotation databases

EnsemblBacteriaiAFN48045; AFN48045; RVBD_0194.
CCP42922; CCP42922; Rv0194.
KBJ41303; KBJ41303; P425_00203.
GeneIDi886790.
KEGGimtu:Rv0194.
mtv:RVBD_0194.
PATRICi18148934. VBIMycTub87468_0220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP42922.1.
CP003248 Genomic DNA. Translation: AFN48045.1.
JLDD01000001 Genomic DNA. Translation: KBJ41303.1.
RefSeqiNP_214708.1. NC_000962.3.
WP_003911097.1. NZ_KK339370.1.

3D structure databases

ProteinModelPortaliO53645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv0194.

Protein family/group databases

TCDBi3.A.1.106.6. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiO53645.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFN48045; AFN48045; RVBD_0194.
CCP42922; CCP42922; Rv0194.
KBJ41303; KBJ41303; P425_00203.
GeneIDi886790.
KEGGimtu:Rv0194.
mtv:RVBD_0194.
PATRICi18148934. VBIMycTub87468_0220.

Organism-specific databases

TubercuListiRv0194.

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
KOiK18894.
OMAiMAVLTYY.
OrthoDBiEOG6T7N3V.
PhylomeDBiO53645.

Enzyme and pathway databases

BioCyciMTBRV:RV0194-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25618 / H37Rv.
  4. "Identification of a novel multidrug efflux pump of Mycobacterium tuberculosis."
    Danilchanka O., Mailaender C., Niederweis M.
    Antimicrob. Agents Chemother. 52:2503-2511(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ANTIBIOTIC RESISTANCE, ENZYME REGULATION.
    Strain: H37Rv.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ATCC 25618 / H37Rv.

Entry informationi

Entry nameiMDREP_MYCTU
AccessioniPrimary (citable) accession number: O53645
Secondary accession number(s): I6Y740, L0T5S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2015
Last sequence update: June 1, 1998
Last modified: February 17, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.