Reviewed, UniProtKB/Swiss-Prot O53615 (GLYA2_MYCTU)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	Rv0070, MT0076
ORF Names:	MTV030.13c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 425	425	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000113619
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O53615-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 224D195C1D8BF680	FASTA	425	45,526
	10 20 30 40 50 60 MNTLNDSLTA FDPDIAALID GELRRQESGL EMIASENYAP LAVMQAQGSV LTNKYAEGYP 70 80 90 100 110 120 GRRYYGGCEF VDGVEQLAID RVKALFGAEY ANVQPHSGAT ANAATMHALL NPGDTILGLS 130 140 150 160 170 180 LAHGGHLTHG MRINFSGKLY HATAYEVSKE DYLVDMDAVA EAARTHRPKM IIAGWSAYPR 190 200 210 220 230 240 QLDFARFRAI ADEVDAVLMV DMAHFAGLVA AGVHPSPVPH AHVVTSTTHK TLGGPRGGII 250 260 270 280 290 300 LCNDPAIAKK INSAVFPGQQ GGPLEHVIAA KATAFKMAAQ PEFAQRQQRC LDGARILAGR 310 320 330 340 350 360 LTQPDVAERG IAVLTGGTDV HLVLVDLRDA ELDGQQAEDR LAAVDITVNR NAVPFDPRPP 370 380 390 400 410 420 MITSGLRIGT PALAARGFSH NDFRAVADLI AAALTATNDD QLGPLRAQVQ RLAARYPLYP ELHRT

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842572 Genomic DNA. Translation: CAA16251.1. AE000516 Genomic DNA. Translation: AAK44300.1.
PIR	G70848.
RefSeq	NP_214584.1. NP_334486.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
ModBase	Search...
Genome annotation databases
GeneID	886983. 922815.
GenomeReviews	Gene locus MT0076 in contig AE000516_GR. Gene locus Rv0070 in contig AL123456_GR.
KEGG	mtc:MT0076.
TIGR	MT0076.
Organism-specific databases
TubercuList	Rv0070.
Phylogenomic databases
HOGENOM	O53615.
OMA	ESAYPIQ.
Enzyme and pathway databases
BRENDA	2.1.2.1. 809.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_MYCTU

Accession

Primary (citable) accession number: O53615

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)