O53597 (RSMG_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.-

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase
Glucose-inhibited division protein B

Gene names

Name:	rsmG
Synonyms:	gidB
Ordered Locus Names:	Rv3919c, MT4038
ORF Names:	MTV028.10c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA By similarity. HAMAP MF_00074
Subcellular location	Cytoplasm Potential HAMAP MF_00074.
Disruption phenotype	Confers low-level streptomycin resistance. Seems to be an important cause of resistance in resistant M.tuberculosis isolates. Ref.3
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Ribosomal RNA small subunit methyltransferase G HAMAP MF_00074		PRO_0000184283
Experimental info
	Sequence conflict	100	1	S → F in AAK48404. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O53597 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B56E8C8BF06A08CA	FASTA	224	23,972
	10 20 30 40 50 60 MSPIEPAASA IFGPRLGLAR RYAEALAGPG VERGLVGPRE VGRLWDRHLL NCAVIGELLE 70 80 90 100 110 120 RGDRVVDIGS GAGLPGVPLA IARPDLQVVL LEPLLRRTES LREMVTDLGV AVEIVRGRAE 130 140 150 160 170 180 ESWVQDQLGG SDAAVSRAVA ALDKLTKWSM PLIRPNGRML AIKGERAHDE VREHRRVMIA 190 200 210 220 SGAVDVRVVT CGANYLRPPA TVVFARRGKQ IARGSARMAS GGTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria." Okamoto S., Tamaru A., Nakajima C., Nishimura K., Tanaka Y., Tokuyama S., Suzuki Y., Ochi K. Mol. Microbiol. 63:1096-1106(2007) [PubMed: 17238915] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842584 Genomic DNA. Translation: CAA16232.1. AE000516 Genomic DNA. Translation: AAK48404.1.
PIR	G70851.
RefSeq	NP_218436.1. NC_000962.2. NP_338590.1. NC_002755.2.
3D structure databases
ProteinModelPortal	O53597.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000932; EBMYCP00000000932; EBMYCG00000000932. EBMYCT00000069972; EBMYCP00000068031; EBMYCG00000069967.
GeneID	886243. 926664.
GenomeReviews	Gene locus MT4038 in contig AE000516_GR. Gene locus Rv3919c in contig AL123456_GR.
KEGG	mtc:MT4038. mtu:Rv3919c.
PATRIC	18130637. VBIMycTub22151_4406.
TIGR	MT4038.
Organism-specific databases
TubercuList	Rv3919c.
Phylogenomic databases
GeneTree	EBGT00050000016057.
HOGENOM	HBG686577.
OMA	VPRLWER.
PhylomeDB	O53597.
ProtClustDB	PRK00107.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G. [Tree]
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
KO	K03501.
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. GidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_MYCTU

Accession

Primary (citable) accession number: O53597

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program