O53597 (RSMG_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase G
EC=2.1.1.-

Alternative name(s):
16S rRNA 7-methylguanosine methyltransferase
Short name=16S rRNA m7G methyltransferase
Glucose-inhibited division protein B

Gene names

Name:	rsmG
Synonyms:	gidB
Ordered Locus Names:	Rv3919c, MT4038
ORF Names:	MTV028.10c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N7 position of guanine in position 518 of 16S rRNA By similarity. HAMAP-Rule MF_00074
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00074.
Disruption phenotype	Confers low-level streptomycin resistance. Seems to be an important cause of resistance in resistant M.tuberculosis isolates. Ref.4
Sequence similarities	Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 15525680. Source: MTBBASE
Molecular_function	rRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Ribosomal RNA small subunit methyltransferase G HAMAP-Rule MF_00074		PRO_0000184283
Regions
	Region	119 – 120	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	74	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	137	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O53597 [UniParc]. Last modified May 29, 2013. Version 2. Checksum: B56E90FBF91A1DCA	FASTA	224	24,032
	10 20 30 40 50 60 MSPIEPAASA IFGPRLGLAR RYAEALAGPG VERGLVGPRE VGRLWDRHLL NCAVIGELLE 70 80 90 100 110 120 RGDRVVDIGS GAGLPGVPLA IARPDLQVVL LEPLLRRTEF LREMVTDLGV AVEIVRGRAE 130 140 150 160 170 180 ESWVQDQLGG SDAAVSRAVA ALDKLTKWSM PLIRPNGRML AIKGERAHDE VREHRRVMIA 190 200 210 220 SGAVDVRVVT CGANYLRPPA TVVFARRGKQ IARGSARMAS GGTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evaluation of alterations responsible for streptomycin resistance in Mycobacterium tuberculosis." Verma J.S., Routela R.S., Rawat D.S., Das R., Manzoor N., Nair D. Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MP 31.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Loss of a conserved 7-methylguanosine modification in 16S rRNA confers low-level streptomycin resistance in bacteria." Okamoto S., Tamaru A., Nakajima C., Nishimura K., Tanaka Y., Tokuyama S., Suzuki Y., Ochi K. Mol. Microbiol. 63:1096-1106(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HQ611141 Genomic DNA. Translation: ADU15870.1. AL123456 Genomic DNA. Translation: CCP46748.1. AE000516 Genomic DNA. Translation: AAK48404.1.
PIR	G70851.
RefSeq	NP_338590.1. NC_002755.2.
3D structure databases
ProteinModelPortal	O53597.
SMR	O53597. Positions 47-183.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3919c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK48404; AAK48404; MT4038.
GeneID	926664.
KEGG	mtc:MT4038. mtu:Rv3919c.
PATRIC	18130637. VBIMycTub22151_4406.
Organism-specific databases
TubercuList	Rv3919c.
Phylogenomic databases
eggNOG	COG0357.
HOGENOM	HOG000221013.
KO	K03501.
OMA	PRLWSRH.
ProtClustDB	PRK00107.
Family and domain databases
HAMAP	MF_00074. 16SrRNA_methyltr_G.
InterPro	IPR003682. rRNA_ssu_MeTfrase_G. [Graphical view]
Pfam	PF02527. GidB. 1 hit. [Graphical view]
PIRSF	PIRSF003078. GidB. 1 hit.
TIGRFAMs	TIGR00138. gidB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMG_MYCTU

Accession

Primary (citable) accession number: O53597
Secondary accession number(s): E7E4Z6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 29, 2013
Last modified:	May 29, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program