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O53446 (FUMC_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2
Gene names
Name:fumC
Synonyms:fum
Ordered Locus Names:Rv1098c, MT1130
ORF Names:MTV017.51c
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743

Subunit structure

Homotetramer By similarity. HAMAP MF_00743

Subcellular location

Cytoplasm By similarity HAMAP MF_00743.

Miscellaneous

There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Fumarate hydratase class II HAMAP MF_00743
PRO_0000161289

Regions

Region128 – 1314B site By similarity
Region138 – 1403Substrate binding By similarity

Sites

Binding site1061Substrate By similarity

Secondary structure

1 ................................................... 474
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O53446 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 4052F93F963D3DBB

FASTA47450,141
        10         20         30         40         50         60 
MAVDADSANY RIEHDTMGEV RVPAKALWRA QTQRAVENFP ISGRGLERTQ IRALGLLKGA 

        70         80         90        100        110        120 
CAQVNSDLGL LAPEKADAII AAAAEIADGQ HDDQFPIDVF QTGSGTSSNM NTNEVIASIA 

       130        140        150        160        170        180 
AKGGVTLHPN DDVNMSQSSN DTFPTATHIA ATEAAVAHLI PALQQLHDAL AAKALDWHTV 

       190        200        210        220        230        240 
VKSGRTHLMD AVPVTLGQEF SGYARQIEAG IERVRACLPR LGELAIGGTA VGTGLNAPDD 

       250        260        270        280        290        300 
FGVRVVAVLV AQTGLSELRT AANSFEAQAA RDGLVEASGA LRTIAVSLTK IANDIRWMGS 

       310        320        330        340        350        360 
GPLTGLAEIQ LPDLQPGSSI MPGKVNPVLP EAVTQVAAQV IGNDAAIAWG GANGAFELNV 

       370        380        390        400        410        420 
YIPMMARNIL ESFKLLTNVS RLFAQRCIAG LTANVEHLRR LAESSPSIVT PLNSAIGYEE 

       430        440        450        460        470 
AAAVAKQALK ERKTIRQTVI DRGLIGDRLS IEDLDRRLDV LAMAKAEQLD SDRL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842575 Genomic DNA. Translation: CAA17214.1.
AE000516 Genomic DNA. Translation: AAK45388.1.
PIRH70896.
RefSeqNP_215614.1. NC_000962.2.
NP_335574.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NO9X-ray2.48A/B/C/D1-474[»]
ProteinModelPortalO53446.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000003944; EBMYCP00000003944; EBMYCG00000003942.
EBMYCT00000071797; EBMYCP00000069856; EBMYCG00000071792.
GeneID885651.
924972.
GenomeReviewsGene locus MT1130 in contig AE000516_GR.
Gene locus Rv1098c in contig AL123456_GR.
KEGGmtc:MT1130.
mtu:Rv1098c.
PATRIC18124246. VBIMycTub22151_1243.
TIGRMT1130.

Organism-specific databases

TubercuListRv1098c.

Phylogenomic databases

GeneTreeEBGT00050000016253.
HOGENOMHBG284369.
OMARIEKDTM.
PhylomeDBO53446.
ProtClustDBPRK00485.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-11948.

Family and domain databases

HAMAPMF_00743. FumaraseC.
[Tree]
InterProIPR005677. Fum_hydII.
IPR018951. Fumarase_C_C.
IPR000362. Fumarate_lyase.
IPR020557. Fumarate_lyase_CS.
IPR008948. L-Aspartase-like.
IPR024083. L-Aspartase-like_N.
IPR022761. Lyase1_N.
[Graphical view]
Gene3DG3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KOK01679.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SUPFAMSSF48557. L-Aspartase-like. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFUMC_MYCTU
AccessionPrimary (citable) accession number: O53446
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 1998
Last modified: January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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