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O53442 (DESA2_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative acyl-[acyl-carrier-protein] desaturase desA2
Short name=DES
EC=1.14.19.-
Alternative name(s):
Putative acyl-ACP desaturase desA2
Gene names
Name:desA2
Ordered Locus Names:Rv1094, MT1126
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Could be involved in mycobacterial fatty acid biosynthesis. Ref.3

Cofactor

Iron.

Pathway

Lipid metabolism; fatty acid metabolism.

Subunit structure

Homodimer. Ref.6

Miscellaneous

Was identified as a high-confidence drug target.

Sequence similarities

Belongs to the fatty acid desaturase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Putative acyl-[acyl-carrier-protein] desaturase desA2
PRO_0000392676

Sites

Metal binding1071Iron
Metal binding1591Iron
Metal binding1891Iron
Metal binding1921Iron

Amino acid modifications

Cross-link145Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Secondary structure

1 ............................. 275
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O53442 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 448D136940150E99

FASTA27531,359
        10         20         30         40         50         60 
MAQKPVADAL TLELEPVVEA NMTRHLDTED IWFAHDYVPF DQGENFAFLG GRDWDPSQST 

        70         80         90        100        110        120 
LPRTITDACE ILLILKDNLA GHHRELVEHF ILEDWWGRWL GRWTAEEHLH AIALREYLVV 

       130        140        150        160        170        180 
TREVDPVANE DVRVQHVMKG YRAEKYTQVE TLVYMAFYER CGAVFCRNLA AQIEEPILAG 

       190        200        210        220        230        240 
LIDRIARDEV RHEEFFANLV THCLDYTRDE TIAAIAARAA DLDVLGADIE AYRDKLQNVA 

       250        260        270 
DAGIFGKPQL RQLISDRITA WGLAGEPSLK QFVTG

« Hide

References

« Hide 'large scale' references
[1]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[2]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[3]"Unique mechanism of action of the thiourea drug isoxyl on Mycobacterium tuberculosis."
Phetsuksiri B., Jackson M., Scherman H., McNeil M., Besra G.S., Baulard A.R., Slayden R.A., DeBarber A.E., Barry C.E. III, Baird M.S., Crick D.C., Brennan P.J.
J. Biol. Chem. 278:53123-53130(2003) [PubMed: 14559907] [Abstract]
Cited for: FUNCTION AS A DESATURASE.
Strain: ATCC 25618 / H37Rv.
[4]"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis."
Raman K., Yeturu K., Chandra N.
BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract]
Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
[5]"Prokayrotic ubiquitin-like protein (Pup) proteome of Mycobacterium tuberculosis."
Festa R.A., McAllister F., Pearce M.J., Mintseris J., Burns K.E., Gygi S.P., Darwin K.H.
PLoS ONE 5:E8589-E8589(2010) [PubMed: 20066036] [Abstract]
Cited for: PUPYLATION AT LYS-145, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: ATCC 25618 / H37Rv.
[6]"X-ray structure of putative acyl-ACP desaturase DesA2 from Mycobacterium tuberculosis H37Rv."
Dyer D.H., Lyle K.S., Rayment I., Fox B.G.
Protein Sci. 14:1508-1517(2005) [PubMed: 15929999] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH IRON, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000516 Genomic DNA. Translation: AAK45384.1.
BX842575 Genomic DNA. Translation: CAA17210.1.
PIRD70896.
RefSeqNP_215610.1. NC_000962.2.
NP_335570.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZA0X-ray2.00A1-275[»]
ProteinModelPortalO53442.
SMRO53442. Positions 8-274.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000003982; EBMYCP00000003982; EBMYCG00000003980.
EBMYCT00000071227; EBMYCP00000069286; EBMYCG00000071222.
GeneID885339.
924977.
GenomeReviewsGene locus MT1126 in contig AE000516_GR.
Gene locus Rv1094 in contig AL123456_GR.
KEGGmtc:MT1126.
mtu:Rv1094.
PATRIC18124238. VBIMycTub22151_1239.
TIGRMT1126.

Organism-specific databases

TubercuListRv1094.

Phylogenomic databases

GeneTreeEBGT00050000016016.
HOGENOMHBG566522.
OMAGYRADTY.
ProtClustDBCLSK790944.

Enzyme and pathway databases

BRENDA1.14.19.2. 3445.

Family and domain databases

InterProIPR005067. Fatty_acid_desaturase-2.
IPR009078. Ferritin/RR-like.
IPR012348. Ribncl_red-rel.
[Graphical view]
Gene3DG3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
KOK03922.
PfamPF03405. FA_desaturase_2. 1 hit.
[Graphical view]
PIRSFPIRSF000346. Dlt9_acylACP_des. 1 hit.
SUPFAMSSF47240. Ferritin/RR_like. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDESA2_MYCTU
AccessionPrimary (citable) accession number: O53442
Secondary accession number(s): Q7D8V3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: June 1, 1998
Last modified: January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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