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O53258 (GATA_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit A
Short name=Glu-ADT subunit A
EC=6.3.5.-
Gene names
Name:gatA
Ordered Locus Names:Rv3011c, MT3091
ORF Names:MTV012.25c
OrganismMycobacterium tuberculosis [Reference proteome] [HAMAP]
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln) By similarity. HAMAP-Rule MF_00120

Catalytic activity

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate. HAMAP-Rule MF_00120

Subunit structure

Heterotrimer of A, B and C subunits By similarity.

Sequence similarities

Belongs to the amidase family. GatA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processgrowth

Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE

translation

Inferred from electronic annotation. Source: HAMAP

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494Glutamyl-tRNA(Gln) amidotransferase subunit A HAMAP-Rule MF_00120
PRO_0000105181

Sites

Active site811Charge relay system By similarity
Active site1561Charge relay system By similarity
Active site1801Acyl-ester intermediate By similarity

Experimental info

Sequence conflict4201M → L in AAK47420. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O53258 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 99A8824ABC436CA6

FASTA49451,438
        10         20         30         40         50         60 
MTDIIRSDAA TLAAKIAIKE VSSAEITRAC LDQIEATDET YHAFLHVAAD EALAAAAAID 

        70         80         90        100        110        120 
KQVAAGEPLP SALAGVPLAL KDVFTTSDMP TTCGSKILEG WRSPYDATLT ARLRAAGIPI 

       130        140        150        160        170        180 
LGKTNMDEFA MGSSTENSAY GPTRNPWNLD RVPGGSGGGS AAALAAFQAP LAIGSDTGGS 

       190        200        210        220        230        240 
IRQPAALTAT VGVKPTYGTV SRYGLVACAS SLDQGGPCAR TVLDTALLHQ VIAGHDPRDS 

       250        260        270        280        290        300 
TSVDAEVPDV VGAARAGAVG DLRGVRVGVV RQLHGGEGYQ PGVLASFEAA VEQLTALGAE 

       310        320        330        340        350        360 
VSEVDCPHFD HALAAYYLIL PSEVSSNLAR FDAMRYGLRV GDDGTRSAEE VMAMTRAAGF 

       370        380        390        400        410        420 
GPEVKRRIMI GTYALSAGYY DAYYNQAQKV RTLIARDLDA AYRSVDVLVS PTTPTTAFRM 

       430        440        450        460        470        480 
GEKVDDPLAM YLFDLCTLPL NLAGHCGMSV PSGLSPDDGL PVGLQIMAPA LADDRLYRVG 

       490 
AAYEAARGPL LSAI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX842581 Genomic DNA. Translation: CAA16096.1.
AE000516 Genomic DNA. Translation: AAK47420.1.
PIRF70856.
RefSeqNP_337606.1. NC_002755.2.

3D structure databases

ProteinModelPortalO53258.
SMRO53258. Positions 9-486.
ModBaseSearch...

Protein-protein interaction databases

STRING83332.Rv3011c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK47420; AAK47420; MT3091.
GeneID923318.
KEGGmtc:MT3091.
mtu:Rv3011c.
PATRIC18128562. VBIMycTub22151_3379.

Organism-specific databases

TubercuListRv3011c.

Phylogenomic databases

eggNOGCOG0154.
HOGENOMHOG000116699.
KOK02433.
OMARYDGVKY.
ProtClustDBPRK00012.

Family and domain databases

Gene3D3.90.1300.10. 1 hit.
HAMAPMF_00120. GatA.
InterProIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERPTHR11895. PTHR11895. 1 hit.
PfamPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMSSF75304. Amidase_sig_enz. 1 hit.
TIGRFAMsTIGR00132. gatA. 1 hit.
PROSITEPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGATA_MYCTU
AccessionPrimary (citable) accession number: O53258
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: May 1, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

SIMILARITY comments

Index of protein domains and families

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