O53231 (DCUP_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen decarboxylase
Short name=UPD
Short name=URO-D
EC=4.1.1.37

Gene names

Name:	hemE
Ordered Locus Names:	Rv2678c, MT2752
ORF Names:	MTV010.02c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III By similarity. HAMAP MF_00218
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2. HAMAP MF_00218
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4. HAMAP MF_00218
Subunit structure	Homodimer By similarity. HAMAP MF_00218
Subcellular location	Cytoplasm Probable HAMAP MF_00218.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00218
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.
Sequence caution	The sequence AAK47067.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: MTBBASE
Molecular function	uroporphyrinogen decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Uroporphyrinogen decarboxylase HAMAP MF_00218		PRO_0000187616
Regions
	Region	30 – 34	5	Substrate binding By similarity
Sites
	Binding site	79	1	Substrate By similarity
	Binding site	154	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	336	1	Substrate By similarity
	Site	79	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	O53231 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: F5F09F1680BDC7B3	FASTA	357	37,606
	10 20 30 40 50 60 MSTRRDLPQS PYLAAVTGRK PSRVPVWFMR QAGRSLPEYR ALRERYSMLA ACFEPDVACE 70 80 90 100 110 120 ITLQPIRRYD VDAAILFSDI VVPLRAAGVD LDIVADVGPV IADPVRTAAD VAAMKPLDPQ 130 140 150 160 170 180 AIQPVLVAAS LLVAELGDVP LIGFAGAPFT LASYLVEGGP SRHHAHVKAM MLAEPASWHA 190 200 210 220 230 240 LMAKLTDLTI AFLVGQIDAG VDAIQVFDSW AGALSPIDYR QYVLPHSARV FAALGEHGVP 250 260 270 280 290 300 MTHFGVGTAE LLGAMSEAVT AGERPGRGAV VGVDWRTPLT DAAARVVPGT ALQGNLDPAV 310 320 330 340 350 VLAGWPAVER AARAVVDDGR RAVDAGAAGH IFNLGHGVLP ESDPAVLADL VSLVHSL

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842580 Genomic DNA. Translation: CAA16021.1. AE000516 Genomic DNA. Translation: AAK47067.1. Different initiation.
PIR	G70869.
RefSeq	NP_217194.1. NC_000962.2. NP_337253.1. NC_002755.2.
3D structure databases
ProteinModelPortal	O53231.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001204; EBMYCP00000001204; EBMYCG00000001204. EBMYCT00000069254; EBMYCP00000067313; EBMYCG00000069249.
GeneID	888934. 925549.
GenomeReviews	Gene locus MT2752 in contig AE000516_GR. Gene locus Rv2678c in contig AL123456_GR.
KEGG	mtc:MT2752. mtu:Rv2678c.
PATRIC	18127790. VBIMycTub22151_2994.
TIGR	MT2752.
Organism-specific databases
TubercuList	Rv2678c.
Phylogenomic databases
GeneTree	EBGT00050000016469.
HOGENOM	HBG628392.
OMA	PRIHFGV.
PhylomeDB	O53231.
ProtClustDB	PRK00115.
Family and domain databases
HAMAP	MF_00218. URO-D. [Tree]
InterPro	IPR006361. Uroporphyrinogen_deCO2ase_HemE. IPR000257. Uroporphyrinogen_deCOase. [Graphical view]
KO	K01599.
PANTHER	PTHR21091:SF2. HemE. 1 hit.
Pfam	PF01208. URO-D. 1 hit. [Graphical view]
TIGRFAMs	TIGR01464. HemE. 1 hit.
PROSITE	PS00906. UROD_1. 1 hit. PS00907. UROD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCUP_MYCTU

Accession

Primary (citable) accession number: O53231

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program