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Protein

Aconitate hydratase A

Gene

acn

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein which binds to selected IRE-like sequences present within the UTRs (untranslated regions) of 3' trxC and 5' IdeR mRNA (PubMed:17384188). Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate (By similarity).By similarity1 Publication

Catalytic activityi

Citrate = isocitrate.1 Publication
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Kineticsi

  1. KM=0.56 mM for isocitrate1 Publication
  1. Vmax=33.3 µmol/min/mg enzyme with isocitrate as substrate1 Publication

pH dependencei

Optimum pH is 8. It retains a high specific activity over a broad pH range.1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (LH57_04880), 2-methylcitrate dehydratase (prpD), Citrate synthase 1 (gltA2), Putative citrate synthase 2 (citA)
  2. Aconitate hydratase A (acn), 2-methylcitrate synthase (prpC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi479 – 4791Iron-sulfur (4Fe-4S)By similarity
Metal bindingi545 – 5451Iron-sulfur (4Fe-4S)By similarity
Metal bindingi548 – 5481Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

  • 2-methylisocitrate dehydratase activity Source: UniProtKB
  • 4 iron, 4 sulfur cluster binding Source: UniProtKB
  • aconitate hydratase activity Source: MTBBASE
  • iron-responsive element binding Source: MTBBASE
  • metal ion binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: UniProtKB
  • mRNA binding Source: UniProtKB

GO - Biological processi

  • growth Source: MTBBASE
  • propionate metabolic process, methylcitrate cycle Source: UniProtKB
  • response to iron ion Source: MTBBASE
  • tricarboxylic acid cycle Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11945.
UniPathwayiUPA00223; UER00718.
UPA00946.

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase A1 Publication (EC:4.2.1.31 Publication)
Short name:
ACN1 Publication
Short name:
Aconitase1 Publication
Alternative name(s):
(2R,3S)-2-methylisocitrate dehydrataseBy similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydrataseBy similarity
Iron-responsive protein-like1 Publication
Short name:
IRP-like1 Publication
Probable 2-methyl-cis-aconitate hydrataseBy similarity (EC:4.2.1.99By similarity)
RNA-binding protein1 Publication
Gene namesi
Name:acn
Ordered Locus Names:Rv1475c, RVBD_1475c
ORF Names:P425_01532
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1475c.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Aconitate hydratase APRO_0000432979Add
BLAST

Proteomic databases

PaxDbiO53166.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv1475c.

Structurei

3D structure databases

ProteinModelPortaliO53166.
SMRiO53166. Positions 13-941.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4107QM5. Bacteria.
COG1048. LUCA.
HOGENOMiHOG000025703.
KOiK01681.
OMAiENLAKWG.
PhylomeDBiO53166.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 2 hits.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O53166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKSVNSFG AHDTLKVGEK SYQIYRLDAV PNTAKLPYSL KVLAENLLRN
60 70 80 90 100
EDGSNITKDH IEAIANWDPK AEPSIEIQYT PARVVMQDFT GVPCIVDLAT
110 120 130 140 150
MREAIADLGG NPDKVNPLAP ADLVIDHSVI ADLFGRADAF ERNVEIEYQR
160 170 180 190 200
NGERYQFLRW GQGAFDDFKV VPPGTGIVHQ VNIEYLASVV MTRDGVAYPD
210 220 230 240 250
TCVGTDSHTT MVNGLGVLGW GVGGIEAEAA MLGQPVSMLI PRVVGFRLTG
260 270 280 290 300
EIQPGVTATD VVLTVTEMLR QHGVVGKFVE FYGEGVAEVP LANRATLGNM
310 320 330 340 350
SPEFGSTAAI FPIDEETIKY LRFTGRTPEQ VALVEAYAKA QGMWHDPKHE
360 370 380 390 400
PEFSEYLELN LSDVVPSIAG PKRPQDRIAL AQAKSTFREQ IYHYVGNGSP
410 420 430 440 450
DSPHDPHSKL DEVVEETFPA SDPGQLTFAN DDVATDETVH SAAAHADGRV
460 470 480 490 500
SNPVRVKSDE LGEFVLDHGA VVIAAITSCT NTSNPEVMLG AALLARNAVE
510 520 530 540 550
KGLTSKPWVK TTIAPGSQVV NDYYDRSGLW PYLEKLGFYL VGYGCTTCIG
560 570 580 590 600
NSGPLPEEIS KAVNDNDLSV TAVLSGNRNF EGRINPDVKM NYLASPPLVI
610 620 630 640 650
AYALAGTMDF DFQTQPLGQD KDGKNVFLRD IWPSQQDVSD TIAAAINQEM
660 670 680 690 700
FTRNYADVFK GDDRWRNLPT PSGNTFEWDP NSTYVRKPPY FEGMTAKPEP
710 720 730 740 750
VGNISGARVL ALLGDSVTTD HISPAGAIKP GTPAARYLDE HGVDRKDYNS
760 770 780 790 800
FGSRRGNHEV MIRGTFANIR LRNQLLDDVS GGYTRDFTQP GGPQAFIYDA
810 820 830 840 850
AQNYAAQHIP LVVFGGKEYG SGSSRDWAAK GTLLLGVRAV IAESFERIHR
860 870 880 890 900
SNLIGMGVIP LQFPEGKSAS SLGLDGTEVF DITGIDVLND GKTPKTVCVQ
910 920 930 940
ATKGDGATIE FDAVVRIDTP GEADYYRNGG ILQYVLRNIL KSG
Length:943
Mass (Da):102,449
Last modified:June 1, 1998 - v1
Checksum:i2403292221124D1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003248 Genomic DNA. Translation: AFN49388.1.
AL123456 Genomic DNA. Translation: CCP44235.1.
JLDD01000018 Genomic DNA. Translation: KBJ34833.1.
RefSeqiNP_215991.1. NC_000962.3.
WP_003898889.1. NC_000962.3.

Genome annotation databases

EnsemblBacteriaiAFN49388; AFN49388; RVBD_1475c.
CCP44235; CCP44235; Rv1475c.
KBJ34833; KBJ34833; P425_01532.
GeneIDi886545.
KEGGimtu:Rv1475c.
mtv:RVBD_1475c.
PATRICi18125112. VBIMycTub22151_1671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003248 Genomic DNA. Translation: AFN49388.1.
AL123456 Genomic DNA. Translation: CCP44235.1.
JLDD01000018 Genomic DNA. Translation: KBJ34833.1.
RefSeqiNP_215991.1. NC_000962.3.
WP_003898889.1. NC_000962.3.

3D structure databases

ProteinModelPortaliO53166.
SMRiO53166. Positions 13-941.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi83332.Rv1475c.

Proteomic databases

PaxDbiO53166.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFN49388; AFN49388; RVBD_1475c.
CCP44235; CCP44235; Rv1475c.
KBJ34833; KBJ34833; P425_01532.
GeneIDi886545.
KEGGimtu:Rv1475c.
mtv:RVBD_1475c.
PATRICi18125112. VBIMycTub22151_1671.

Organism-specific databases

TubercuListiRv1475c.

Phylogenomic databases

eggNOGiENOG4107QM5. Bacteria.
COG1048. LUCA.
HOGENOMiHOG000025703.
KOiK01681.
OMAiENLAKWG.
PhylomeDBiO53166.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00718.
UPA00946.
BioCyciMetaCyc:MONOMER-11945.

Family and domain databases

Gene3Di3.20.19.10. 1 hit.
3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
InterProiIPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015928. Aconitase/3IPM_dehydase_swvl.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
IPR000573. AconitaseA/IPMdHydase_ssu_swvl.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 4 hits.
PfamiPF00330. Aconitase. 1 hit.
PF00694. Aconitase_C. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF52016. SSF52016. 1 hit.
SSF53732. SSF53732. 2 hits.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACNA_MYCTU
AccessioniPrimary (citable) accession number: O53166
Secondary accession number(s): F2GES4, I6XY12, Q7D8D8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2015
Last sequence update: June 1, 1998
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.