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Protein

Aconitate hydratase A

Gene

acn

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. The apo form of AcnA functions as a RNA-binding regulatory protein which binds to selected IRE-like sequences present within the UTRs (untranslated regions) of 3' trxC and 5' IdeR mRNA (PubMed:17384188). Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate (By similarity).By similarity1 Publication

Catalytic activityi

Citrate = isocitrate.1 Publication
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Kineticsi

  1. KM=0.56 mM for isocitrate1 Publication
  1. Vmax=33.3 µmol/min/mg enzyme with isocitrate as substrate1 Publication

pH dependencei

Optimum pH is 8. It retains a high specific activity over a broad pH range.1 Publication

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.1 Publication
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (LH57_04880), 2-methylcitrate dehydratase (prpD), Citrate synthase 1 (gltA2), Putative citrate synthase 2 (citA)
  2. Aconitate hydratase A (acn), 2-methylcitrate synthase (prpC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi479Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi545Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi548Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

  • 2-methylisocitrate dehydratase activity Source: UniProtKB
  • 4 iron, 4 sulfur cluster binding Source: UniProtKB
  • aconitate hydratase activity Source: MTBBASE
  • iron-responsive element binding Source: MTBBASE
  • metal ion binding Source: UniProtKB-KW
  • mRNA 3'-UTR binding Source: UniProtKB
  • mRNA binding Source: UniProtKB

GO - Biological processi

  • growth Source: MTBBASE
  • propionate metabolic process, methylcitrate cycle Source: UniProtKB
  • response to iron ion Source: MTBBASE
  • tricarboxylic acid cycle Source: UniProtKB

Keywordsi

Molecular functionLyase, RNA-binding
Biological processTricarboxylic acid cycle
LigandIron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:G185E-5659-MONOMER
UniPathwayiUPA00223; UER00718
UPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase A1 Publication (EC:4.2.1.31 Publication)
Short name:
ACN1 Publication
Short name:
Aconitase1 Publication
Alternative name(s):
(2R,3S)-2-methylisocitrate dehydrataseBy similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydrataseBy similarity
Iron-responsive protein-like1 Publication
Short name:
IRP-like1 Publication
Probable 2-methyl-cis-aconitate hydrataseBy similarity (EC:4.2.1.99By similarity)
RNA-binding protein1 Publication
Gene namesi
Name:acn
Ordered Locus Names:Rv1475c, RVBD_1475c
ORF Names:P425_01532
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv1475c

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: MTBBASE
  • cytosol Source: MTBBASE
  • extracellular region Source: MTBBASE
  • plasma membrane Source: MTBBASE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004329791 – 943Aconitate hydratase AAdd BLAST943

Proteomic databases

PaxDbiO53166

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi83332.Rv1475c

Structurei

3D structure databases

ProteinModelPortaliO53166
SMRiO53166
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4107QM5 Bacteria
COG1048 LUCA
HOGENOMiHOG000025703
KOiK01681
OMAiMRIIPPG
PhylomeDBiO53166

Family and domain databases

Gene3Di3.20.19.10, 1 hit
InterProiView protein in InterPro
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR006249 Aconitase/IRP2
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PANTHERiPTHR11670 PTHR11670, 1 hit
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 2 hits
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

O53166-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSKSVNSFG AHDTLKVGEK SYQIYRLDAV PNTAKLPYSL KVLAENLLRN
60 70 80 90 100
EDGSNITKDH IEAIANWDPK AEPSIEIQYT PARVVMQDFT GVPCIVDLAT
110 120 130 140 150
MREAIADLGG NPDKVNPLAP ADLVIDHSVI ADLFGRADAF ERNVEIEYQR
160 170 180 190 200
NGERYQFLRW GQGAFDDFKV VPPGTGIVHQ VNIEYLASVV MTRDGVAYPD
210 220 230 240 250
TCVGTDSHTT MVNGLGVLGW GVGGIEAEAA MLGQPVSMLI PRVVGFRLTG
260 270 280 290 300
EIQPGVTATD VVLTVTEMLR QHGVVGKFVE FYGEGVAEVP LANRATLGNM
310 320 330 340 350
SPEFGSTAAI FPIDEETIKY LRFTGRTPEQ VALVEAYAKA QGMWHDPKHE
360 370 380 390 400
PEFSEYLELN LSDVVPSIAG PKRPQDRIAL AQAKSTFREQ IYHYVGNGSP
410 420 430 440 450
DSPHDPHSKL DEVVEETFPA SDPGQLTFAN DDVATDETVH SAAAHADGRV
460 470 480 490 500
SNPVRVKSDE LGEFVLDHGA VVIAAITSCT NTSNPEVMLG AALLARNAVE
510 520 530 540 550
KGLTSKPWVK TTIAPGSQVV NDYYDRSGLW PYLEKLGFYL VGYGCTTCIG
560 570 580 590 600
NSGPLPEEIS KAVNDNDLSV TAVLSGNRNF EGRINPDVKM NYLASPPLVI
610 620 630 640 650
AYALAGTMDF DFQTQPLGQD KDGKNVFLRD IWPSQQDVSD TIAAAINQEM
660 670 680 690 700
FTRNYADVFK GDDRWRNLPT PSGNTFEWDP NSTYVRKPPY FEGMTAKPEP
710 720 730 740 750
VGNISGARVL ALLGDSVTTD HISPAGAIKP GTPAARYLDE HGVDRKDYNS
760 770 780 790 800
FGSRRGNHEV MIRGTFANIR LRNQLLDDVS GGYTRDFTQP GGPQAFIYDA
810 820 830 840 850
AQNYAAQHIP LVVFGGKEYG SGSSRDWAAK GTLLLGVRAV IAESFERIHR
860 870 880 890 900
SNLIGMGVIP LQFPEGKSAS SLGLDGTEVF DITGIDVLND GKTPKTVCVQ
910 920 930 940
ATKGDGATIE FDAVVRIDTP GEADYYRNGG ILQYVLRNIL KSG
Length:943
Mass (Da):102,449
Last modified:June 1, 1998 - v1
Checksum:i2403292221124D1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003248 Genomic DNA Translation: AFN49388.1
AL123456 Genomic DNA Translation: CCP44235.1
JLDD01000018 Genomic DNA Translation: KBJ34833.1
RefSeqiNP_215991.1, NC_000962.3
WP_003898889.1, NZ_KK339370.1

Genome annotation databases

EnsemblBacteriaiAFN49388; AFN49388; RVBD_1475c
CCP44235; CCP44235; Rv1475c
KBJ34833; KBJ34833; P425_01532
GeneIDi886545
KEGGimtu:Rv1475c
mtv:RVBD_1475c
PATRICifig|83332.111.peg.1642

Similar proteinsi

Entry informationi

Entry nameiACNA_MYCTU
AccessioniPrimary (citable) accession number: O53166
Secondary accession number(s): F2GES4, I6XY12, Q7D8D8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2015
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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