Reviewed, UniProtKB/Swiss-Prot O53163 (ECH12_MYCTU)

Names and origin

Protein names

Recommended name:
    Probable enoyl-CoA hydratase echA12
    EC=4.2.1.17

Gene names

Name:	echA12
Synonyms:	fadB2
Ordered Locus Names:	Rv1472, MT1518
ORF Names:	MTV007.19

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could possibly oxidize fatty acids using specific components By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	enoyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Probable enoyl-CoA hydratase echA12		PRO_0000109341

Sequences

	Sequence		Length	Mass (Da)
	O53163-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 6542FC79E7164F1C	FASTA	285	30,575
	10 20 30 40 50 60 MPHRCAAQVV AGYRSTVSLV LVEHPRPEIA QITLNRPERM NSMAFDVMVP LKEALAQVSY 70 80 90 100 110 120 DNSVRVVVLT GAGRGFSPGA DHKSAGVVPH VENLTRPTYA LRSMELLDDV ILMLRRLHQP 130 140 150 160 170 180 VIAAVNGPAI GGGLCLALAA DIRVASSSAY FRAAGINNGL TASELGLSYL LPRAIGSSRA 190 200 210 220 230 240 FEIMLTGRDV SAEEAERIGL VSRQVPDEQL LDACYAIAAR MAGFSRPGIE LTKRTLWSGL 250 260 270 280 DAASLEAHMQ AEGLGQLFVR LLTANFEEAV AARAEQRAPV FTDDT

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842576 Genomic DNA. Translation: CAA16000.1. AE000516 Genomic DNA. Translation: AAK45783.1.
PIR	C70873.
RefSeq	NP_215988.1. NP_335969.1.
3D structure databases
SMR	O53163. Positions 7-284.
ModBase	Search...
Genome annotation databases
GeneID	886547. 924454.
GenomeReviews	Gene locus MT1518 in contig AE000516_GR.
KEGG	mtc:MT1518. mtu:Rv1472.
TIGR	MT1518.
Organism-specific databases
TubercuList	Rv1472.
Phylogenomic databases
HOGENOM	HBG748731.
OMA	LEGHMQA.
Enzyme and pathway databases
BRENDA	4.2.1.17. 809.
Family and domain databases
InterPro	IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ECH12_MYCTU

Accession

Primary (citable) accession number: O53163

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)