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Protein

Ornithine carbamoyltransferase, catabolic

Gene

arcB

Organism
Lactobacillus sakei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine degradation via ADI pathway

This protein is involved in step 2 of the subpathway that synthesizes carbamoyl phosphate from L-arginine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Arginine deiminase (arcA), Arginine deiminase (arcA), Arginine deiminase (arcA)
  2. Ornithine carbamoyltransferase, catabolic (arcB)
This subpathway is part of the pathway L-arginine degradation via ADI pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from L-arginine, the pathway L-arginine degradation via ADI pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121Carbamoyl phosphateBy similarity
Binding sitei74 – 741Carbamoyl phosphateBy similarity
Binding sitei85 – 851Carbamoyl phosphateBy similarity
Binding sitei109 – 1091Carbamoyl phosphateBy similarity
Binding sitei167 – 1671OrnithineBy similarity
Binding sitei231 – 2311OrnithineBy similarity
Binding sitei299 – 2991Carbamoyl phosphateBy similarity
Binding sitei317 – 3171Carbamoyl phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Arginine metabolism

Enzyme and pathway databases

UniPathwayiUPA00254; UER00365.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase, catabolic (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:arcB
OrganismiLactobacillus sakei
Taxonomic identifieri1599 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Ornithine carbamoyltransferase, catabolicPRO_0000112938Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi314315.LSA0371.

Structurei

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Important for structural integrityBy similarity
Sitei148 – 1481Important for structural integrityBy similarity

3D structure databases

ProteinModelPortaliO53089.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 625Carbamoyl phosphate bindingBy similarity
Regioni135 – 1384Carbamoyl phosphate bindingBy similarity
Regioni235 – 2362Ornithine bindingBy similarity
Regioni271 – 2744Carbamoyl phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O53089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNSVFQGRS LLAEKDFTKS ELEYLIDFSL HLKDLKKKGI PHHYLEGKNI
60 70 80 90 100
ALLFEKNSTR TRAAFTTAAI DLGAHPEFLG KNDIQLGKKE SVEDTAKVLG
110 120 130 140 150
SMFDGIEFRA LAKVVEDLAK YSGVPVWNGL TDEWHPTQMI ADFMTVKENF
160 170 180 190 200
GKLKGVTLTY VGDGRNNMAN SLLVTGSMLG VNIHIVAPDS LQPTQEVRDL
210 220 230 240 250
AEGYAKETGS KNMITSDVDA GVKGSDVLYT DVWVSMGEED KFEERVNLLK
260 270 280 290 300
PYQINMDMVK KTGNENMIIM HCLPAFHDIE TEYGKKIDEQ FGIQEMEITD
310 320 330
EAFRSKYARQ FEEAENRMHS IKAIMAATLG NLFIPQA
Length:337
Mass (Da):37,774
Last modified:June 1, 1998 - v1
Checksum:i1C252EB5D0342D53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001330 Genomic DNA. Translation: CAA04683.1.
PIRiT46742.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001330 Genomic DNA. Translation: CAA04683.1.
PIRiT46742.

3D structure databases

ProteinModelPortaliO53089.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi314315.LSA0371.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.

Enzyme and pathway databases

UniPathwayiUPA00254; UER00365.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Structural and functional analysis of the gene cluster encoding the enzymes of the arginine deiminase pathway of Lactobacillus sakei."
    Zuniga M., Champomier-Verges M.-C., Zagorec M., Perez-Martinez G.
    J. Bacteriol. 180:4154-4159(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiOTCC_LACSK
AccessioniPrimary (citable) accession number: O53089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: June 1, 1998
Last modified: November 11, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.