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Protein

Type 4 prepilin-like proteins leader peptide-processing enzyme

Gene

orf2

Organism
Escherichia coli
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue.UniRule annotation

Catalytic activityi

Typically cleaves a -Gly-|-Phe- bond to release an N-terminal, basic peptide of 5-8 residues from type IV prepilin, and then N-methylates the new N-terminal amino group, the methyl donor being S-adenosyl-L-methionine.UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, MethyltransferaseUniRule annotation, ProteaseUniRule annotation, Transferase

Protein family/group databases

MEROPSiA24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Type 4 prepilin-like proteins leader peptide-processing enzymeUniRule annotation (EC:2.1.1.-UniRule annotation, EC:3.4.23.43UniRule annotation)
Gene namesi
Name:orf2Imported
OrganismiEscherichia coliImported
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei91 – 110HelicalSequence analysisAdd BLAST20
Transmembranei116 – 132HelicalSequence analysisAdd BLAST17
Transmembranei144 – 162HelicalSequence analysisAdd BLAST19
Transmembranei182 – 209HelicalSequence analysisAdd BLAST28

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Interactioni

Protein-protein interaction databases

STRINGi155864.L7043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 88DiS_P_DiSInterPro annotationAdd BLAST75
Domaini99 – 206Peptidase_A24InterPro annotationAdd BLAST108

Sequence similaritiesi

Belongs to the peptidase A24 family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixSequence analysis

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.

Sequencei

Sequence statusi: Complete.

O53004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLERRWRREA LTLLAHSVPE SGPAFNLMIP QSHCPCCFHP LGIRDNIPLL
60 70 80 90 100
SYLFLKGKSR CCGERIPVYY PLVEITNSVL FILAASRFPP GLTLAAAWLF
110 120 130 140 150
ISMLLVLAVI DCHTALLPDV LTLPLLWLGL LFSLQRSVVT LEEAVVGAVS
160 170 180 190 200
GYLCLWGLYW LFRFATGREG LGYGDFKLAA ALGAWMGWQA LPSILLFASV
210 220 230
SGLIVTVLLR IVTATDFTRP LPFGPWLALS GVCHFLLM
Length:238
Mass (Da):26,318
Last modified:June 1, 1998 - v1
Checksum:i6F2226714AB89181
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07545 Genomic DNA. Translation: CAA68830.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y07545 Genomic DNA. Translation: CAA68830.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.L7043.

Protein family/group databases

MEROPSiA24.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105EHH. Bacteria.
COG1989. LUCA.

Family and domain databases

InterProiIPR010627. Pept_A24A_N.
IPR014032. Peptidase_A24A_bac.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06750. DiS_P_DiS. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
PRINTSiPR00864. PREPILNPTASE.
ProtoNetiSearch...

Entry informationi

Entry nameiO53004_ECOLX
AccessioniPrimary (citable) accession number: O53004
Entry historyi
Integrated into UniProtKB/TrEMBL: June 1, 1998
Last sequence update: June 1, 1998
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Multifunctional enzymeUniRule annotation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.