O52951 (KITH_BACNA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:

tdk

Encoded on

Plasmid pLS32

Organism

Bacillus subtilis subsp. natto

Taxonomic identifier

86029 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis

Protein attributes

Sequence length	195 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00124
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000175057
Regions
	Nucleotide binding	8 – 15	8	ATP By similarity
	Nucleotide binding	86 – 89	4	ATP By similarity
Sites
	Active site	87	1	Proton acceptor Potential
	Metal binding	146	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity
	Metal binding	184	1	Zinc By similarity
	Metal binding	187	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	O52951 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 1ABFA791B1A56B23	FASTA	195	22,221
	10 20 30 40 50 60 MDITFNTGLM GSGKSKKLID DYLIDPKEKV ALSVSLTEDT FSRGKIESRD GRSLRSINLN 70 80 90 100 110 120 RDQFKQNISL LEIIIFMTNT QTIYIDESQF LPKETVEKFV SLSESYHVPI HFYGLDLTFT 130 140 150 160 170 180 GELFDSSSHL LTILPSENIN RISRGCEASK CSKIAQYNAR IVDGKVSRSG ETFVEEKSYY 190 LALCSDHYYN DEKII

References

[1]	"A novel Bacillus natto plasmid pLS32 capable of replication in Bacillus subtilis." Tanaka T., Ogura M. FEBS Lett. 422:243-246(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM 1163.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D49467 Genomic DNA. Translation: BAA24875.1.
RefSeq	YP_004243650.1. NC_015149.1.
3D structure databases
ProteinModelPortal	O52951.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	10227784.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase. Divergent sequence.
InterPro	IPR027417. P-loop_NTPase. IPR001267. Thymidine_kinase. [Graphical view]
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_BACNA

Accession

Primary (citable) accession number: O52951

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	June 1, 1998
Last modified:	May 29, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program